iBB
58.2K views | +0 today
Follow
iBB
Institute for Bioengineering and Biosciences
Curated by iBB
Your new post is loading...
Your new post is loading...
Scoop.it!

Extracellular Vesicles and Infection

Extracellular Vesicles and Infection | iBB | Scoop.it

Extracellular vesicles (EVs) are small membrane bound structures released by cells into the extracellular space. They have been shown to transport different molecules such as proteins, nucleic acids and lipids to other cells, serving as vehicles of intracellular communication. EVs have also been shown to play important roles during viral and bacterial infection. Viruses can hijack the biogenesis of EVs to promote viral spreading. Additionally, these EVs are also important mediators in inflammation and immune responses during both bacterial and viral infections. A recent publication in the journal Pharmaceutics, by Diogo Gonçalves, Sandra Pinto and Fábio Fernandes (from iBB/IST), reviews these mechanisms and described the impact of bacterial EVs in regulating immune. Finally, the review also focuses on the potential and challenges of using EVs to tackle infectious diseases.

iBB's insight:

Check full paper here

No comment yet.
Scoop.it!

The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death

The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death | iBB | Scoop.it

The bacterial protein azurin shows an unexpected therapeutic effect against various types of cancer. This property seems to result from its unique structural and surface features. A 28-residue peptide (named p28) derived from the middle part of azurin has been subjected to various studies and reached two clinical trials phase I in US. In a recent paper published in Biomedicines, a iBB team (Ana Rita Garizo, Lígia Coelho, Sandra Pinto, Tiago Dias, Fábio Fernandes, Nuno Bernardes and Arsénio M Fialho) were able to identified another anticancer bioactive peptide (CT-p19LC) derived from the C-terminal of azurin. CT-p19LC proved to interact preferentially with cancer cells, causing a significative inhibition of cell proliferation in a dose dependent manner. Moreover, it is proposed that the mode of action of CT-p19LC involves perturbation or disruption of cancer cell membranes. Overall this study highlights the relevance of azurin as a source of bioactive peptides with potential application in cancer therapies.

No comment yet.
Scoop.it!

Yeast Response to Acetic Acid Involves Pdr18-mediated Ergosterol Transport at the Membrane

Yeast Response to Acetic Acid Involves Pdr18-mediated Ergosterol Transport at the Membrane | iBB | Scoop.it

The ability of Saccharomyces cerevisiae to overcome the stress induced by cytotoxic compounds depends on the activity of plasma membrane transporters of the ABC superfamily, presumably through the questionable unspecific efflux of multiple drugs and xenobiotic compounds. A recent paper by iBB researchers provides new insights into the biological role of the ABC transporter of the pleiotropic drug resistance family of putative drug efflux pumps Pdr18, proposed to mediate ergosterol incorporation in plasma membrane. Pdr18 expression was found to help cells to counteract acetic acid-induced decrease of plasma membrane lipid order, increase the non-specific membrane permeability and decrease the transmembrane electrochemical potential. Results support the notion that Pdr18-mediated multistress resistance is linked to the status of plasma membrane lipid environment related with ergosterol content and the associated plasma membrane properties. The paper, published in Scientific Reports, results from the PhD project of Cláudia Godinho, advised by Prof. Isabel Sá-Correia from BSRG-iBB in collaboration with Fábio Fernandes and Sandra Pinto from BSIRG-iBB).

No comment yet.
Scoop.it!

Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement

Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement | iBB | Scoop.it

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is crucial to many cellular processes in eukaryotes, including membrane trafficking, signal transduction, ion channel function  and cytoskeleton dynamics. This function multiplicity is partially achieved through a dynamic spatiotemporal organization of PI(4,5)P2 within the membrane. In a recent paper published in IJMS, an IBB team (Maria J. Sarmento, Luís Borges-Araújo, Sandra N.Pinto, Nuno Bernardes, Joana Ricardo, Ana Coutinho, Manuel Prieto and Fábio Fernandes) was able to quantify PI(4,5)P2 confinement in living cells making use of FRET imaging measurements. PI(4,5)P2 was found to be significantly compartmentalized at the plasma membrane of HeLa cells. These PI(4,5)P2 enriched domains were shown to not depend on cholesterol content, ruling out an association with lipid rafts. On the other hand, upon inhibition of actin polymerization, compartmentalization of PI(4,5)P2 was almost entirely eliminated, confirming that the cytoskeleton network is the critical component responsible for the formation of nanoscale PI(4,5)P2 domains.

No comment yet.
Scoop.it!

Azurin Perturbs Lipid Rafts’ Organization and Enhances Sensitivity to Anti-cancer Drugs

Azurin Perturbs Lipid Rafts’ Organization and Enhances Sensitivity to Anti-cancer Drugs | iBB | Scoop.it

A new determinant of the interaction of the bacterial anti-cancer protein azurin with the lipid rafts present in the membrane of cancer cells has been uncovered by a team led by Arsénio Fialho from BSRG-iBB, in collaboration with Sandra Pinto and Fábio Fernandes from CQFM-IN and iBB. The lipid rafts in these cells contribute to increase membrane order, rigidity and resistance to anti-cancer drugs. As a consequence of the interaction with lipid rafts, we demonstrate that treating cells with azurin increases membrane fluidity, and ultimately benefits the action of other drugs, probably by facilitating its entry in cancer cells. The work was recently published in the journal Cell Cycle.

No comment yet.