Increasing Yields of Cytochrome B5 in Bioreactors | iBB |

The production of recombinant proteins is gaining increasing importance as several applications request high quality proteins. However, several process parameters affect both the growth of cells and product yields. In a paper recently published in the journal Molecules, Ricardo FS Pereira and Carla CCR de Carvalho (iBB-BERG) used high throughput systems and statistical methods to assess the influence of fermentation conditions in lab-scale bioreactors. Using partial least squares, it was found that the height-to-diameter ratio of the bioreactor, aeration rate, and PID controller parameters contributed significantly to the final biomass and cytochrome concentrations. This information was used to fine-tune the process parameters, which increased cytochrome production and yield several-fold.