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Institute for Bioengineering and Biosciences
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Continuous Aqueous Two-phase Extraction

Continuous Aqueous Two-phase Extraction | iBB | Scoop.it

Aqueous two-phase extraction (ATPE) can bring numerous benefits to the downstream processing of biological products, including high recovery yields, high selectivity and relatively low cost. With biomanufacturing entering an era where continuous processing is regarded as the key solution to increase flexibility and decrease costs, the intrinsic characteristics of ATPE and high degree of integration, makes it highly suitable for continuous processing. In a recent paper published in Fluid Phase Equilibria, Diogo Faria and colleagues from BERG-IBB make a brief review of the history of continuous ATPE, analyse the main devices used in continuous extraction, and discuss the key and future challenges in the development of continuous processes.

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1st CODOBIO Consortium Meeting

1st CODOBIO Consortium Meeting | iBB | Scoop.it

The first consortium meeting of the EU-funded ITN CODOBIO: Continuous Downstream Processing of Biologics was held on the 22nd October at IST, Lisbon. The consortium involves 20 participants from Academia, Industry and Regulatory Agencies. Thirty two members were present in the meeting including the 15 Early Stage Researchers. Amin Javidanbardan and Malik Wahab from BERG-iBB had the opportunity to present their PhD projects, which focus on the miniaturization of continuous bioprocesses using microfluidics.

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Effect of Fluorescent Tags on Rapid Screening of IgG Partition in PEG-salt ATPS

Effect of Fluorescent Tags on Rapid Screening of IgG Partition in PEG-salt ATPS | iBB | Scoop.it

Rapid empirical optimization/screening of the partition of biomolecules in aqueous two-phase systems (ATPS) can be performed in microfluidic devices coupled with fluorescence microscopy. However, the effect of fluorescent tags on partition must be carefully evaluated to assess the validity of extrapolating the results to a situation where the molecules are unlabelled. To evaluate this influence, researchers from BERG-iBB and INESC-MN (Mariana São Pedro, Ana Azevedo, Maria Raquel Aires-Barros and Ruben Soares) carried out an ATPS study using both microtubes (batch) and microfluidic devices (continuous). IgG molecules were labelled with three fluorescent tags with different global charge and reactivity, namely BODIPY™ FL maleimide, Alexa Fluor® 430 NHS ester and BODIPY™ FL NHS ester. The thiol reactive BODIPY™ FL maleimide coupled with a reducing agent provided minimal bias relative to the native molecule in PEG-phosphate systems. The work was recently published in the Biotechnology Journal.

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Daniel Silva Defends PhD Thesis in Biotechnology and Biosciences

Daniel Silva Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Daniel Filipe Camarneiro Silva will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, wednesday the 12th December 2018 (10:30 H, room PA3). During the last years, and under the supervision of Raquel Aires Barros from BERG-iBB and Virginia Chu from INESC-MN, Daniel focused on the development of aqueous two phase separation of monoclonal antibodies in microfluidic devices. The title of his thesis is "Microfluidic separation using aqueous two phase systems”.

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Inês Pinto Defends PhD Thesis in Biotechnology and Biosciences

Inês Pinto Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Inês Pinto will be defending her PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, monday the 24th September 2018 (9:30 H, room PA3). During the last years, and under the supervision of Profs. Ana Azevedo and Raquel Barros from BERG-iBB, and João Pedro Conde from INESC-MN, Inês developed a microfluidic platform for the rapid screening of the chromatographic purification of monoclonal antibodies. The title of her thesis is "Screening of multimodal ligands for the capture and polishing of antibody streams: A microfluidic approach for rapid optimization of chromatographic operating conditions”.

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Inês Pinto Wins ESBES 2018 Award

Inês Pinto Wins ESBES 2018 Award | iBB | Scoop.it

Inês Pinto from BERG-iBB just won the ESBES Award at the ESBES 2018 conference held in Lisbon (8-12 September 2018) for her work “Optimizing the performance of chromatographic separations using integrated microfluidic devices: adsorption/elution kinetic studies and multiplexed analysis”. The work is part of Inês' PhD project on Biotechnology and is co-authored by Raquel Aires Barros, Viginia Chu, João Pedro Conde and Ana Azevedo. Diogo Pinto from SCERG was one of the runnerups of the competition. The ESBES Award is granted biannually by the European Society of Biochemical Engineering Sciences and aims at recognizing outstanding PhD thesis or scientific publications by students or early carrier researchers in the field of biochemical engineering.

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Sub-minute Detection of Multiple Food Contaminants With a Lab-on-a-chip Device

Sub-minute Detection of Multiple Food Contaminants With a Lab-on-a-chip Device | iBB | Scoop.it

The 3-fold increase in global population within the last 70 years implies a demand for an equally fast paced global food trade. Thus, to assure high food safety standards and protect the consumers, simple, rapid and effective means of screening food contaminants are required to adequately enforce regulations. Towards this goal, researchers at BERG-iBB and INESC MN recently developed a lab-on-a-chip microfluidic device with integrated photodetectors, allowing the simultaneous immunodetection of three harmful fungal toxins in a single step, in less than one minute and at concentrations in the parts-per-billion range. The work was published in the Royal Society of Chemistry journal Lab on a Chip.

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Studies on the Purification of Antibody Fragments

Studies on the Purification of Antibody Fragments | iBB | Scoop.it
Antigen-binding fragments (Fabs) are becoming increasingly prevalent as an alternative to full-length monoclonal antibodies. In this work, researchers from BERG-iBB (Ana Azevedo and Raquel Aires-Barros) in collaboration INESC-MN (João Pedro conde and Virginia Chu), studied the recovery of the Fab fragments through different downstream purification schemes using a miniaturized platform anchored on a microfluidic chromatography lab-on-a-chip. This work is part of André Nascimento´s PhD thesis in Biotechnology and Biosciences. Click on title to learn more in the journal Separation and Purification Technology.
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Lisbon Hosts ESBES 2018

Lisbon Hosts ESBES 2018 | iBB | Scoop.it

The 12th Symposium of the European Symposium on Biochemical Engineering Sciences will be held in Lisbon, Portugal from 9 – 12 September 2018. ESBES 2018 will provide a platform for bioengineers and others to discuss the latest in research and industrial application of all aspects of biochemical engineering. The event is organized by BERG-iBB members Raquel Aires-Barros and Ana Azevedo. The deadline for abstract submissions is 16th february 2018. Click on title to learn more.

iBB's insight:
An excellent opportunity to present iBB´s research in the field!
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Process Simulation and Economic Analysis of mAbs Purification

Process Simulation and Economic Analysis of mAbs Purification | iBB | Scoop.it

Monoclonal antibodies are expensive to produce in part due to their purification process, which is centered in protein-A affinity chromatography. A recent publication by Ana Azevedo and co-workers from BERG-iBB highlights a novel purification strategy for mAbs comprising phenylboronic acid multimodal chromatography for capture followed by polishing by ion exchange monolithic chromatography and packed bed hydrophobic interaction chromatography. Process simulation and economical analysis show that the new process is worth investing in and could present a viable alternative to the platform process used by most industrial players. Click on title to learn more.

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A Microfluidic Toolbox for the Optimization of Aqueous Two Phase Antibody Extraction

A Microfluidic Toolbox for the Optimization of Aqueous Two Phase Antibody Extraction | iBB | Scoop.it
The use of aqueous two-phase extraction (ATPE) is a viable option for the purification of antibodies and other vluable proteins, but these systems are difficult to model and optimize. In a publication in the Journal of Chmoratography A, researchers at BERG-iBB and INESC-MN describe a methodology for the rapid screening of antibody extraction conditions that relies on a microfluidic channel-based toolbox. A first microfluidic structure allows a simple negative-pressure driven rapid screening of up to 8 extraction conditions simultaneously, using less than 20 μL of each phase-forming solution per experiment, while a second microfluidic structure allows the integration of multi-step extraction protocols based on the results obtained with the first device. Click on title to learn more.
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BERG Research Presented at the ACS Spring Meeting 2017

BERG Research Presented at the ACS Spring Meeting 2017 | iBB | Scoop.it

BERG research in the Downstream Processing and Bioprocess Miniaturization topics is being presented at the ACS spring meeting 2017 in S. Francisco (2-6 April 2017), Division of Biochemical Technology. The talks by Raquel Aires-Barros and Ana Azevedo are entitled "Rapid determination of partition coefficients of biomolecules in a microfluidic ATPS platform using fluorescence microscopy" and "Rapid optimization of chromatographic separations using a nano-liter scale column in a microfluidic platform", respectively. (Click on title to access the conference site).

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Protein Microarray-on-a-chip Coupled to Micro Photodetectors for Point-of-use Mycotoxin Detection

Protein Microarray-on-a-chip Coupled to Micro Photodetectors for Point-of-use Mycotoxin Detection | iBB | Scoop.it
Researchers at INESC MN and BERG-iBB recently published the development of a microfluidic device to simultaneously screen a panel of toxins in multiple samples. This interdisciplinary work combines biochemistry, physics and materials engineering to (1) generate a reproducible array of 9 discrete immunosensing regions in an area of 0.12 mm2, (2) achieve the required sensitivities and (3) integrate an array of microfabricated Si photoconductors to acquire the signal of each discrete region. This type of device holds promise towards the rapid screening of key harmful mycotoxins in foods and feeds, assuring an effective enforcement of EU regulations. Click on title to learn more.
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Oscillatory Reactor for Continuous ATPS Presented at ICB IV

Oscillatory Reactor for Continuous ATPS Presented at ICB IV | iBB | Scoop.it

Diogo Faria, PhD candidate at BIOTECnico Programme in IBB-BERG, gave a poster presentation at the conference "Integrated Continuous Biomanufacturing IV", organized by ECI (Engineering Conferences International), held in Cape Cod, Massachusetts, in October 6-9. The poster was about Continuous Aqueous Two-Phase System Extraction using Oscillatory Flow Reactor. The work was carried out under the supervision of Prof. Ana Azevedo (IBB-BERG) and Prof. Raquel Aires Barros (iBB-BERG), with colaboration of António Ferreira (FEUP).

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Optimizing the Performance of Chromatographic Separations Using Microfluidics

Optimizing the Performance of Chromatographic Separations Using Microfluidics | iBB | Scoop.it

The establishment of novel, simpler and improved purification processes is extremely important to make antibody-based treatment options more accessible and with a lower economic burden to healthcare systems and patients. In this context, the development of microfluidic tools for the optimization of chromatography operating conditions can offer advantages such as low consumption of molecules, fast generation of results and ability to automate and multiplex the assays. In a collaboration between INESC-MN and iBB, researchers have recently reported a novel microfluidics-based methodology to perform rapid and multiplexed screening of various chromatography ligands relative to their ability to bind different target molecules in solution. The work was published in the Biotechnology Journal and is expected to contribute to the fields of separation sciences and analytical chemistry and inspire new developments within the purification of biopharmaceuticals or other molecules.

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Maria João Jacinto to Defend PhD Thesis in Bioengineering

Maria João Jacinto to Defend PhD Thesis in Bioengineering | iBB | Scoop.it

Maria João Jacinto will be defending her PhD thesis in Bioengineering at Instituto Superior Técnico, thursday the 7th March 2019 (10:30 H, room PA3). During the last years, and under the supervision of Raquel Barros and Ana Azevedo from BERG-iBB and Richard Willson from the University of Houston, Maria João focused her efforts on the development of platforms for the downstream processing and detection of VLPs and bacteriophages. The title of her thesis is "Integrative platforms for downstream processing and detection of viral particles”.

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Rúben Soares Defends PhD Thesis in Biotechnology and Biosciences

Rúben Soares Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Rúben Soares will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, monday the 15th October 2018 (10:00 H, room PA3). During the last years, and under the supervision of Profs. João Pedro Conde from INESC-MN and Raquel Barros from BERG-iBB, Rúben worked on the development of several miniaturized modules (analyte preparation, immunodetection, optical transduction and portable eçlectronics) of a portable point-of-need mycotoxin screening chip. The title of his thesis is "Development of integrated microfluidic mycotoxin screening chip for point-of-need food safety applications”.

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ESBES 2018: Lisbon at the Forefront of Biochemical Engineering

ESBES 2018: Lisbon at the Forefront of Biochemical Engineering | iBB | Scoop.it

The 12th European Symposium on Biochemical Engineering Sciences was held in Lisbon from 9 to 12 of September of 2018 at the Calouste Gulbenkian Foundation. This year, the European Society of Biochemical Engineering Sciences (ESBES) organized the event with the collaboration of iBB/IST, SPBT and Dechema. Raquel Aires-Barros, the event chair, and Ana Azevedo, co-chair, mobilized a team of 15 PhD and MSc students from IST to receive 324 participants (75% non-portuguese). As a complement to scientific lectures, the event featured workshops on Modelling, Monitoring, Measurement and Control (M3C), Scientific Writing and Bioengineering Education. The last topic was extensively discussed during the Symposium as a possible emergent section of ESBES to address and promote an active cooperation between European academia and industry. Click on the title for more information on ESBES 2018.

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iBB Integrates ITN Awarded on "Continuous Downstream Processing of Biologics"

iBB Integrates ITN Awarded on "Continuous Downstream Processing of Biologics" | iBB | Scoop.it

The European Project CODOBIO: Continuous Downstream Processing of Biologics has been recommended for funding by the European Commission under a Marie Sklodowska Curie Action – ITN - Innovative Training Network. The consortium involves 20 participants form Academia, Industry and Regulatory Agencies. The goal is to develop a new generation of multidisciplinary young researchers fit to work in academia and industry in the field of continuous manufacturing of biologics with special emphasis on continuous downstream processing. The research and training program will cover all technical and management skills required for the implementation of continuous manufacturing in biotechnological industry. The IST team is composed by Ana Azevedo, Raquel Aires-Barros, Miguel Prazeres and Guilherme Ferreira from BERG-iBB.

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LYTAG-driven Purification Strategies for Monoclonal Antibodies

LYTAG-driven Purification Strategies for Monoclonal Antibodies | iBB | Scoop.it

Monoclonal antibodies are becoming a leading class of biopharmaceuticals but to increase their accessibility to the general population, new production processes must be developed in particular for the downstream processing. In this publication, researchers from BERG-iBB (Ana Azevedo and Raquel Aires-Barros) in collaboration with the biotechnology companies Biomedal S.L. (Seville, Spain) and ChiPro GmbH (Bremen, Germany) propose an alternative and innovative affinity chromatographic purification method using quaternary amine anion-exchange matrices. Separation is driven by the dual affinity ligand LYTAG-Z, composed of a choline binding polypeptide (LYTAG) and the synthetic antibody binding Z domain. Click on title to learn more.

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Purification of M13 Bacteriophage With Ionic Liquids

Purification of M13 Bacteriophage With Ionic Liquids | iBB | Scoop.it

Bacteriophage M13 is widely used in phage display technology and bionanotechnology, and is considered a possible antibacterial therapeutic agent, among other applications. However, conventional phage purification involves many steps, with high costs and significant product loss (~ 60%). Researchers at BERG-iBB, in collaboration with Isabel Marrucho from Centro de Química Estrutural and João Gonçalves from iMed, propose a scalable M13 bacteriophage purification process using a novel stationary phase based on a polymeric ionic liquid (PIL) with a positively charged backbone structure. The work is part of Maria João Jacinto´s PhD thesis in Bioengineering. Click on title to learn more in Journal of Chromatography A.

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Chromatography-on-a-Chip

Chromatography-on-a-Chip | iBB | Scoop.it

Optimization of chromatography is a lengthy and demanding task that requires large volumes of reagents and costly equipment. Ana Azevedo and colleagues from BERG-iBB, in collaboration with João Pedro Conde from INESC-MN, describe an integrated, reusable and portable microfluidic platform for the rapid screening of chromatographic conditions. The platform was tested for the capture of fluorophore-labeled monoclonal antibodies from cell culture supernatants using a multimodal ligand (Capto MMC). The work is part of Inês Pinto´s PhD thesis in Biotechnology and Biosciences. Click on title to leran more in the journal Sensors and Actuators B.

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Ana Azevedo Receives Outstanding Poster Award at Integrated Continuous Biomanufacturing Conference

Ana Azevedo Receives Outstanding Poster Award at Integrated Continuous Biomanufacturing Conference | iBB | Scoop.it
Ana Azevedo has received the outstanding poster award at the Integrated Continuous Biomanufacturing III for her communication entitled "Continuous Extraction Strategies For Monoclonal Antibodies: From Macro- To Micro-scale ". This project arises from a collaboration between Ana Azevedo and Raquel Barros from BERG-iBB and João Pedro Conde from INESC-MN. The ECI Conference took place in Cascais, the 17-21 September 2017.
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Sample Preparation With Aqueous Two-Phase Systems for On-site Immunodetection of Mycotoxins

Sample Preparation With Aqueous Two-Phase Systems for On-site Immunodetection of Mycotoxins | iBB | Scoop.it
Researchers at BERG-iBB and INESC-MN recently published a simple and versatile sample preparation methodology based on a green aqueous two-phase system for the simultaneous extraction and concentration of mycotoxins in multiple feeds, which are currently among the most relevant and widespread contaminants in the food and feed industry. This method was tailored for the subsequent rapid detection of ochratoxin A, aflatoxin B1 and deoxynivalenol at the relevant limits within the ng/g range, using a microfluidic multiplexed chemiluminescent immunoassay. Overall, sample preparation and detection were achieved in about 25 min using a “point-of-need” compatible process. Click on title to learn more.
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iBB-BERG Represented at ISPPP 2016

iBB-BERG Represented at ISPPP 2016 | iBB | Scoop.it
iBB was recently represented at the International Symposium ISPPP 2016 by researchers from BERG's Bioseparation Engineering Lab, who presented 3 oral communications (Raquel Aires-Barrros, Ana Azevedo and Emanuel Capela) and 2 poster communications (Sara Rosa and Inês Pinto).
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