iBB
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iBB
Institute for Bioengineering and Biosciences
Curated by iBB
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Rapid Formation of Mineralized Bone Tissue Based on Stem Cell-mediated Osteogenesis

Rapid Formation of Mineralized Bone Tissue Based on Stem Cell-mediated Osteogenesis | iBB | Scoop.it

Bone regeneration, following fracture, relies on autologous and allogenic bone grafts. However, majority of fracture population consists of older individuals with poor quality bone associated with loss and/or modification of matrix proteins critical for bone formation and mineralization. Allografts sufer from same limitations and carry the risk of delayed healing, infection, immune rejection and eventual fracture. Researchers from SCERG-iBB, working in collaboration with colleagues from the Rensselaer Polytechnic Institute in the US, have applied a synergistic biomimetic strategy to develop matrices that rapidly form bone tissue. Collagen matrices, enhanced with osteocalcin and/or osteopontin, increased the rate and quantity of synthesized bone matrix by increasing mesenchymal stem/stromal (MSC) cell proliferation, accelerating osteogenic diferentiation and enhancing angiogenesis The work was published in Scientific Reports.

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Expansion of Human Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors

Expansion of Human Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors | iBB | Scoop.it

The successful use of Human induced Pluripotent Stem Cells (hiPSC) for disease modelling, drug discovery and, ultimately, for regenerative therapies depends on the development of robust bioprocesses capable of generating large numbers of hiPSC and derivatives. SCERG-iBB researchers developed a bioprocess for the scalable generation of hiPSC in a microcarrier-based system using, for the first time, single-use Vertical-Wheel bioreactors. hiPSC culture was performed in working volumes up to 300 mL, maintaining the pluripotency and genomic integrity of the cells, providing an important tool for the successful manufacturing of hiPSC-based products.  The work was published in the Journal of Chemical Technology and Biotechnology.

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Structural Maturation of Human Cardiomyocytes Derived from Pluripotent Stem Cells

Structural Maturation of Human Cardiomyocytes Derived from Pluripotent Stem Cells | iBB | Scoop.it

Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) have an enormous potential for in vitro modeling of cardiac diseases and for testing the effect and toxicity of new drugs. However, when compared to adult CMs, hiPSC-CMs are very immature, exhibiting low structural development and functionality. SCERG-iBB researchers developed a novel methodological framework to quantify structural aspects of hiPSC-CMs during long-term culture. hiPSC-CMs showed significant progression in several structural characteristics namely cardiomyocyte fiber density and length. Importantly, this methodology contributes to set new metrics to develop applications for drug screening and disease modeling for hiPSC-CMs. The research has been published on Biochemical and Biophysical Research Communications.

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Engineering Human MSC with VEGF-encoding Minicircles for Angiogenic Gene Therapy

Engineering Human MSC with VEGF-encoding Minicircles for Angiogenic Gene Therapy | iBB | Scoop.it

Peripheral artery disease (PAD) is a debilitating condition characterized by the blockage of arteries, which leads to limb amputation in more severe cases. Researchers from SCERG- and BERG-iBB propose the use of human bone marrow (BM) MSC transiently transfected with minicircles encoding for VEGF as an ex vivo gene therapy strategy to enhance angiogenesis in PAD patients. The data shows that VEGF overexpression improved the angiogenic potential of MSC in vitro, as confirmed by endothelial cell tube formation and cell migration assays.These results suggest that minicircle-mediated VEGF gene delivery, combined with the unique properties of human MSC, could represent a promising ex vivo gene therapy approach to improve angiogenesis in the context of PAD. The work was published in Human Gene Therapy.

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Neural Induction of Human Induced Pluripotent Stem Cells for Neurodevelopmental Toxicity Studies

Neural Induction of Human Induced Pluripotent Stem Cells for Neurodevelopmental Toxicity Studies | iBB | Scoop.it

The ability to differentiate neural progenitors (NP) from human induced pluripotent stem cells (hiPSCs) provides an opportunity to develop new applications for cellular therapy, disease modelling and drug screening. SCERG-iBB researchers developed a platform that can be applied towards the study of the effect of neurotoxic molecules that impair normal embryonic development, such as the antiepileptic drug valproic acid (VPA). It was verified that exposure to VPA led to a prevalence of NP structures over neuronal differentiation, confirmed by analysis of the expression of neural cell adhesion molecule, and neural rosette number and morphology. This methodology can potentially complement current toxicity tests for the detection of teratogenic compounds that can interfere with normal embryonic development. The work was published in Toxicology Letters.

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