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Institute for Bioengineering and Biosciences
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Fed-batch Production of L-Asparaginase by Recombinant Pichia pastoris

Fed-batch Production of L-Asparaginase by Recombinant Pichia pastoris | iBB | Scoop.it

L-Asparaginase (ASNase) used in the treatment of acute lymphoblastic leukemia is produced from bacterial sources only. BERG-iBB researchers working with colleagues from U. São Paulo, Kings College London, U. Génova and U. Campinas developed a recombinant Pichia pastoris to synthesise ASNase II from Saccharomyces cerevisiae. A multistage fed-batch cultivation divided in four distinct phases was applied to produce ASNase II and determine fermentation parameters.Results showed that the best strategy employed for ASNase II production uses glycerol fed-batch phase with pseudo exponential feeding plus induction with continuous methanol feeding. The study was published in Frontiers in Bioengineering and Biotechnology.

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Ana Pfluck Defends PhD Thesis in Biotechnology and Biosciences

Ana Pfluck Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it
Ana Carolina Dolvitsch Pfluck will be defending her Ph. D. thesis in Biotechnology and Biosciences at Instituto Superior Técnico, friday the 15th december 2017 (10:00 H, room PA3). During the last years, and under the supervision of Luís Fonseca from BERG-iBB, Ana studied the enzymatic synthesis of polyesters. The title of Ana's thesis is ”Polyester Synthesis Using Enzyme: A Case Study of Enzymatic Stability, Biocatalysis and Selection of the Reaction Media”.
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Rui Carvalho Defends PhD Thesis in Biotechnology and Biosciences

Rui Carvalho Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it
Rui Nuno Leitão de Carvalho will be defending his Ph D thesis in Biotechnology and Biosciences at Instituto Superior Técnico, thursday the 20th july 2017 (9:30 H, salão nobre). During the last years, and under the supervision of Luís Fonseca from BERG-iBB, Rui focused his efforts on the development of ion jellly-based materials for bioenergy applications. The title of Rui's thesis is “Design of Ionic liquid-based biomaterials for development of a new generation of Biofuel Cells”.
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Fátima Pinto Defends PhD Thesis in Bioengineering

Fátima Pinto Defends PhD Thesis in Bioengineering | iBB | Scoop.it

Fátima Pinto will be defending her PhD thesis in Bioengineering at Instituto Superior Técnico, wednesday the 10th October 2018 (15:30 H, room PA3). During the last years, and under the supervision of Luís Fonseca and Dragana Barros from BERG-iBB, Fátima focused her attention on the development and characterization of biodegradable nanocarriers to be incorporated in pharmaceutical, nutraceutical or cosmeceutical formulations for the targeted release of bioactive substances. The title of her thesis is "Design and Characterization of Lipid Nanoparticles for an Effective Topical Administration of Active Compounds”.

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Enzymatic Synthesis of Alkyl Esters in Miniemulsions

Enzymatic Synthesis of Alkyl Esters in Miniemulsions | iBB | Scoop.it

Oil-in-water miniemulsions display potential for application as a “green” system in the enzymatic alkyl ester synthesis. BERG-iBB researchers led by Luís Fonseca combined miniemulsion systems with medium engineering to increase the yields and produtivity of the cutinase-catalysed synthesis of medium-chain alkyl esters. Enzyme stability and reutilization were tuned by operating in a fed-batch mode, which allowed long-term utilization of the free enzyme. Click on title to learn more.


Photo details: 3D structure of cutinase from F. Solani pisi with catalytic triad in blue (PDB code: 1AGY).

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Use of Silk Fibroin Functionalized with CuSO4 in Microwave-Assisted Knoevenagel Condensation

Use of Silk Fibroin Functionalized with CuSO4 in Microwave-Assisted Knoevenagel Condensation | iBB | Scoop.it

Conventional organic synthesis has greatly contributed to the production of a huge number of molecular precursors of essential materials in modern life. However, these methods are characterized by a strong negative impact when evaluated in terms of sustainability. In the last decade, microwave radiation techniques have been able to perform many of these organic chemistry reactions generally faster, with better yields, and according to the principles of Green Chemistry. In a paper recently published by Luis Fonseca from BERG-iBB and colleagues from university of S. Paulo, silk fibroin fibers functionalized with CuSO4 were tested as a renewable catalyst in combination with microwave radiation to assist Knoveneganel condensation reactions due to its great potential for forming carbon-carbon bonds from a wide range of compounds. Click on title to learn more.

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