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Topical Collection "Microbial Virulence Factors"

Topical Collection "Microbial Virulence Factors" | iBB | Scoop.it

Jorge H. Leitão is the editor of a topical collection entitled Microbial Virulence Factors for the journal International Journal of Molecular Sciences (IJMS), after the success guest-edition of 2 special issues in 2019 and 2020 on the same topic. IJMS (ISSN 1422-0067; CODEN: IJMCFK; ISSN 1661-6596 for printed edition) is an international, peer-reviewed, open access journal providing an advanced forum for biochemistry, molecular and cell biology, molecular biophysics, molecular medicine, and all aspects of molecular research in chemistry, and is published semimonthly online by MDPI.  The topical collection is open to submissions. Research papers, up-to-date review articles, and commentaries are all welcome.

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Comparative Genomics and Evolutionary Analysis of RNA-Binding Proteins of Burkholderia cenocepacia J2315

Comparative Genomics and Evolutionary Analysis of RNA-Binding Proteins of Burkholderia cenocepacia J2315 | iBB | Scoop.it

RNA-binding proteins (RBPs) are important regulators of cellular functions, playing critical roles on the survival of bacteria and in the case of pathogens, on their interaction with the host. RBPs are involved in transcriptional, post-transcriptional, and translational processes. However, except for model organisms like Escherichia coli, there is little information about the identification or characterization of RBPs in other bacteria, namely in members of the Burkholderia cepacia complex (Bcc). Bcc is a group of bacterial species associated with a poor clinical prognosis in cystic fibrosis patients. These species have some of the largest bacterial genomes, and except for the presence of two-distinct Hfq-like proteins, their RBP repertoire has not been analyzed so far. Using in silico approaches, we identified 186 conventional putative RBPs in Burkholderia cenocepacia J2315, an epidemic and multidrug resistant pathogen of cystic fibrosis patients. In a work recently published in the journal GENES, Joana Feliciano, António Seixas, Tiago Pita and Jorge H. Leitão described the comparative genomics and phylogenetic analysis of RBPs present in multiple copies and predicted to play a role in transcription, protein synthesis, and RNA decay in Bcc bacteria. The analysis suggest the existence, in B. cenocepacia and in other Bcc bacteria, of some extra and unexplored functions for the mentioned RBPs, as well as of alternative mechanisms involved in RNA regulation and metabolism in these bacteria.

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Antimicrobial Activity of Silver Camphorimine Complexes against Candida Strains

Antimicrobial Activity of Silver Camphorimine Complexes against Candida Strains | iBB | Scoop.it

The synthesis of hydroxide [Ag(OH)L] (L =I VL, VL, VIL, VIIL), oxide [{AgL}2}(μ-O)] (L = IL, IIL, IIIL, VL, VIL) or chloride [AgIIL]Cl, [Ag(VIL)2]Cl complexes, obtained from reactions of mono- or bicamphorimine derivatives with Ag(OAc) or AgCl, appeared in a recent publication on the journal Antibiotics, resulting from a collaborative research involving the iBB members Jorge H. Leitão, Nuno Mira, Ana Rego and Sílvia Sousa; M. Fernanda Carvalho from CQE; and Fernanda Marques from C2TN. The new complexes were characterized by spectroscopic (NMR, FTIR) and elemental analysis. X-ray photoelectron spectroscopy (XPS), ESI mass spectra and conductivity measurements were undertaken to corroborate formulations. The antimicrobial activity of complexes and some ligands were evaluated towards Candida albicans and Candida glabrata, and strains of the bacterial species Escherichia coli, Burkholderia contaminans, Pseudomonas aeruginosa and Staphylococcus aureus based on the Minimum Inhibitory Concentrations (MIC). A significant feature of these redesigned complexes is their ability to sensitize C. albicans, a trait that was not found for the previously investigated [Ag(NO3)L] complexes. The MIC values of the complexes towards bacteria were in the range of those of [Ag(NO3)L] and well above those of the precursors Ag(OAc) or AgCl. The activity of the complexes towards normal fibroblasts V79 was evaluated by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Results showed that the complexes have a significant cytotoxicity.

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Ag(I) Camphor Complexes: Antimicrobial Activity by Design

Ag(I) Camphor Complexes: Antimicrobial Activity by Design | iBB | Scoop.it

The synthesis, characterization and antibacterial activity of eleven new complexes of general formula [Ag(NO3)(L-Y)2] corresponding to Ag(I) camphorimine complexes and a camphor sulfonylimine complex were reported in a recent publication in Journal of Inorganic Biochemistry, resulting from collaborative work of JH Leitão from BSRG and MF Carvalho from CQE. The study was performed to gain insights into structure/antimicrobial activity relationships. The lipophilicity and polarity which are important parameters concerning the biological activity of the complexes are also high dependent of the characteristics of the camphor ligands. The redox properties of the complexes studied by cyclic voltammetry showed that their reduction potentials are essentially independent of their electronic and steric properties. The antibacterial activity of all the complexes, against Gram-positive (S. aureus Newman) and Gram-negative (Escherichia coli ATCC25922, Pseudomonas aeruginosa 477, Burkholderia contaminans IST408) strains was evaluated through calculation of MIC values. Results show that complexes with camphor imine ligands that combine high lipophilicity with low dipolar moment exhibit enhanced antibacterial activity. The ability to establish hydrogen bonding emerged as an important contribution to the antibacterial activity of the camphor sulphonylimine complex.

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Small Noncoding Regulatory RNAs from Pseudomonas aeruginosa and Burkholderia cepacia Complex

Small Noncoding Regulatory RNAs from Pseudomonas aeruginosa and Burkholderia cepacia Complex | iBB | Scoop.it

Cystic fibrosis (CF) is the most life-limiting autosomal recessive disorder in Caucasians, with chronic bacterial airway infections representing the major cause of early decease. Pseudomonas aeruginosa and bacteria from the Burkholderia cepacia complex (Bcc) are the leading pathogens of CF patients’ airways. A wide array of virulence factors is responsible for infections caused by these bacteria, which have tightly regulated responses to the host environment. Small noncoding RNAs (sRNAs) are major regulatory molecules in these bacteria. Several approaches have been developed to study P. aeruginosa sRNAs, many of which are involved in the virulence. On the other hand, the knowledge on Bcc sRNAs remains far behind. In a review published on International Journal of Molecular Sciences, Tiago Pita, Joana Feliciano and Jorge H. Leitão from BSRG-iBB updated the knowledge on characterized sRNAs involved in P. aeruginosa virulence, compiled data so far achieved on sRNAs from the Bcc and discuss their possible roles on bacteria virulence.

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Advancing the Development of Vaccines Against Bacteria of the Burkholderia cepacia complex

Advancing the Development of Vaccines Against Bacteria of the Burkholderia cepacia complex | iBB | Scoop.it

 Bacteria of the Burkholderia cepacia complex (Bcc) remain an important cause of morbidity and mortality among patients suffering from cystic fibrosis. Eradication of these pathogens by antimicrobial therapy often fails, highlighting the need to develop novel strategies to eradicate infections. Vaccines are attractive since they can confer protection to particularly vulnerable patients, as is the case of cystic fibrosis patients. Several studies have identified specific virulence factors and proteins as potential subunit vaccine candidates. So far, no vaccine is available to protect from Bcc infections. In a recent publication in the journal Vaccines, iBB-BSRG researchers Sílvia Sousa, António M. Seixas and Jorge H. Leitão review the most promising postgenomic approaches and selected web tools available to speed up the identification of immunogenic proteins with the potential of conferring protection against Bcc infections.

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The International Microorganism Day was a Success!

The International Microorganism Day was a Success! | iBB | Scoop.it
The International Microorganism Day, organized and sponsored by The Portuguese Society of Microbiology (SPM), the "Ordem dos Biólogos", UNESCO and Ciência Viva, was celebrated on the 17th September 2017, at Pavilhão do Conhecimento, Lisbon. The Department of Bioengineering of Instituto Superior Técnico and the Biological Sciences Research Group (BSRG) of iBB played an key role in the initiative. Organized by Jorge Leitão, BSRG’s contribution included the promotion of 3 activities that attracted the curiosity of around 1000 visitors of all ages: “Artisanal Beer”, “Gellan, a microbial gum” and “Bioinformatics”. Beer production was highlighted by Margarida Palma and “Clube de Cervejeiros do IST”. BSRG’s partner company “Cerveja artesanal d´Ourique” offered its beers for degustation. The use of bacterial gums in the food, pharma and cosmetic industries, and the analysis of DNA and protein sequences by bioinformatic tools were also on display with the aid of students from Núcleo de Engenharia Biológica of IST. Prof. Isabel Sá-Correia, SPM President, was also present at the event.
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Joana Feliciano Defends PhD Thesis in Biotechnology and Biosciences

Joana Feliciano Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it
Joana Rita Rodrigues Feliciano will be defending her Ph D thesis in Biotechnology and Biosciences at Instituto Superior Técnico, monday the 27th march 2017 (10:30 H, room PA1). During the last years, and under the supervision of Jorge Leitão from BSRG-IBB, Joana focused her efforts on the study of the virulence mechanisms of the Burkholderia cepacia complex. The title of Joana's thesis is “Towards the unveiling of the multifarious virulence mechanisms of the Burkholderia cepacia complex: exopolysaccharide biosynthesis, RNA chaperones, and development of an animal infection model”.
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Burkholderia cepacia Complex Virulence Gene Expression Regulation: A Review

Burkholderia cepacia Complex Virulence Gene Expression Regulation: A Review | iBB | Scoop.it

Burkholderia cepacia complex (Bcc) bacteria emerged as opportunistic pathogens in cystic fibrosis and immunocompromised patients. Their eradication is very difficult due to the high level of intrinsic resistance to clinically relevant antibiotics. Bcc bacteria have large and complex genomes, composed of two to four replicons, with variable numbers of insertion sequences. The complexity of Bcc genomes confers a high genomic plasticity to these bacteria, allowing their adaptation and survival to diverse habitats, including the human host. In a recent paper, members of the BSRG reviewed recent studies performed using omics approaches to elucidate in vivo adaptive strategies and virulence gene regulation expression of Bcc bacteria when infecting the human host or subject to conditions mimicking the stressful environment of the cystic fibrosis lung. Click on title to learn more.

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Antibacterial Activity of Silver Camphorimine Coordination Polymers

Antibacterial Activity of Silver Camphorimine Coordination Polymers | iBB | Scoop.it

In a paper recently published in Dalton Transactions, researchers from CQE/IST and BSRG-iBB describe the synthesis and full characterization of five new silver camphorimine complexes of general formula [Ag(NO3)YL] with bactericidal properties. The structure of [Ag(NO3)(OC10H14NC6H4NC10H14O)] (III) was analyzed using single crystal X-ray diffraction, showing that it arranges as a coordination polymer formed by sequential Ag(NO3) units bridged by the bi-camphor ligand (2AL). The antimicrobial properties of the new complexes were screened using the disk diffusion method and their Minimal Inhibitory Concentrations (MIC) were assessed against selected bacterial strains of the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli, Pseudomonas aeruginosa, and Burkholderia contaminans. The lowest MICs were observed for III, with estimated values of 72, 20, 32 and 19 μg mL−1 for S. aureus, E. coli, B. contaminans, and P. aeruginosa, respectively. In the case of S. aureus, similar MIC values were obtained for silver nitrate and compound III. All five compounds were bactericidal when used in concentrations equal or above the MIC value, as found by enumerating the total colony forming units (CFUs) after incubation in their presence.

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Hfq: A Multifaceted RNA Chaperone Involved in Virulence

Hfq: A Multifaceted RNA Chaperone Involved in Virulence | iBB | Scoop.it

The Hfq protein has emerged in recent years as a master regulator of gene expression in bacteria, mainly due to its ability to mediate the interaction of small noncoding RNAs with their mRNA targets,
including those related to virulence in Gram-negative bacteria. In a paper recently published in Future Microbiology, researchers from iBB/BSRG reviewed current knowledge on the involvement of Hfq in the regulation of virulence traits related to secretion systems, alternative sigma factors, outer membrane proteins, polysaccharides and iron metabolism. Recent data from transcriptomics and proteomics studies performed for major pathogens were included. Current knowledge on how Hfq protein impacts pathogenicity of bacterial pathogensis also summarized and correlated. Click on title to learn more.

 

Photo details: Hfq protein with a bound sRNA., Bob Plaag, Public Domain, https://en.wikipedia.org/wiki/Hfq_protein

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The B. cenocepacia sRNA MtvR Regulates Hfq in E. coli and P. aeruginosa

The B. cenocepacia sRNA MtvR Regulates Hfq in E. coli and P. aeruginosa | iBB | Scoop.it

MtvR is a 136-nucleotide long sRNA previously identified in the human pathogen Burkholderia cenocepacia J2315 and with homologues restricted to bacteria of the Burkholderia cepacia complex. In a paper published in PlosOne, a team led by Jorge Leitão from BSRG show that MtvR negatively regulates the hfq mRNA levels in both bacterial species. In the case of E. coli, this negative regulation is shown to involve binding of MtvR to the 5′-UTR region of the hfqEc mRNA. Results presented also show that expression of MtvR in E. coli and P. aeruginosa originates multiple phenotypes, including reduced resistance to selected stresses, biofilm formation ability, and increased susceptibility to various antibiotics. Click on title to learn more.

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Key Parameters on the Antibacterial Activity of Silver Camphor Complexes

Key Parameters on the Antibacterial Activity of Silver Camphor Complexes | iBB | Scoop.it

A recent publication in the journal Antibiotics describes the synthesis and analytical and spectroscopical characterization of nine new complexes with camphor imine or camphor sulfonimine ligands were, aiming to identify the key parameters that drive the antibacterial activity of the complexes with metal cores and imine substituents with distinct electronic and steric characteristics. The antimicrobial activity of all complexes was evaluated by determining their minimum inhibitory concentrations (MIC) against the Gram-negative Escherichia coli ATCC25922, Pseudomonas aeruginosa 477, and Burkholderia contaminans IST408, and the Gram-positive Staphylococcus aureus Newman. All the complexes display higher activity towards Gram-negative strains than towards the Gram-positive strain. This work results from the collaborative research between the research groups of Fernanda Carvalho from CQE and Jorge H. Leitão from BSRG-IBB. The paper was selected as “Editor´s Choice”.

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Investigations into the Structure/Antibacterial Activity Relationships of Cyclam and Cyclen Derivatives

Investigations into the Structure/Antibacterial Activity Relationships of Cyclam and Cyclen Derivatives | iBB | Scoop.it

In a paper recently published in the journal Antibiotics, a series of cyclam- and cyclen-derived salts were designed and studied as antibacterials by the research groups headed by JH Leitão (BSRG) and AM Martins and LG Alves (CQE). The compounds were designed specifically to gain insights into their structure and antibacterial activity towards Staphylococcus aureus and Escherichia coli, used respectively, as Gram-positive and Gram-negative model organisms. The newly synthesized compounds are monosubstituted and trans-disubstituted tetraazamacrocycles that display benzyl, methylbenzyl, trifluoromethylbenzyl, or trifluoroethylbenzyl substituents appended on the nitrogen atoms of the macrocyclic ring. The results obtained show that the chemical nature, polarity, and substitution patterns of the benzyl groups, as well as the number of pendant arms, are critical parameters for the antibacterial activity of the cyclam-based salts. The paper was published as part of the journal Special Issue New Insights into Antibacterial Compounds: From Synthesis and Discovery to Molecular Mechanisms of Action, guest-edited by Jorge H. Leitão.

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Special Issue on “New insights into antibacterial compounds"

Special Issue on “New insights into antibacterial compounds" | iBB | Scoop.it

Jorge H. Leitão from BSRG-iBB is guest-editing a special issue for the open-access journal "Antibiotics” entitled “New insights into antibacterial compounds: from synthesis and discovery to molecular mechanisms of action” . This issue aims to gather papers describing novel antibiotics, originating form chemical synthesis, repositioning of existent drugs, or from natural sources like plant extracts, herbs and spices. Papers on the description of novel structures, discovery of novel targets and mechanisms of action, as well as on the use of Omics approaches in the field of novel antibiotics discovery and characterization are also welcome.

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New Insights into the Immunoproteome of B. cenocepacia From Cystic Fibrosis Patients

New Insights into the Immunoproteome of B. cenocepacia From Cystic Fibrosis Patients | iBB | Scoop.it

Bacteria of the Burkholderia cepacia complex (Bcc) are ubiquitous multidrug resistant organisms and opportunistic pathogens capable of causing life threatening lung infections among cystic fibrosis (CF) patients. No effective therapies are available to eradicate Bcc bacteria from CF patients, as these organisms are inherently resistant to most clinically available antimicrobials. In a collaborative research work headed by BSRG members JH Leitão and SA Sousa, in collaboration with the CBMA (UMinho) team headed by Pedro Santos, and the Cystic Fibrosis center (HSM) headed by MDs C Barreto and L Pereira, an immunoproteomics approach was used to identify Bcc proteins that stimulate the humoral immune response of the CF host. The work was published in the journal New Biotechnology and reports for the first time the identification of 19 proteins as immunogenic. Ten proteins were predicted as extracytoplasmic, 9 of them being conserved in Bcc genomes. The immunogenic Bcc extracytoplasmic proteins are potential targets for development of novel therapeutic strategies and diagnostic tools to protect patients against the onset of chronic Bcc lung infections.

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Antimicrobial Properties of Camphor-Derived Silver Complexes

Antimicrobial Properties of Camphor-Derived Silver Complexes | iBB | Scoop.it

The emergence of bacterial resistance to available antimicrobials has prompted the search for novel antibacterial compounds to overcome this public health problem. Metal-based complexes have been much less explored than organic compounds as antimicrobials, leading to investigations of the antimicrobial properties of selected complexes in which silver may occupy the frontline due to its use as medicine since ancient times. Like silver, camphor has also long been used for medicinal purposes. However, in both cases, limited information exists concerning the mechanisms of their antimicrobial action. In a recent collaborative work by the research group headed by Jorge H. Leitão from BSRG/iBB and M. Fernanda Carvalho from CQE, the present knowledge of the antimicrobial properties of camphor-derived silver complexes is reviewed, focusing on recent research on the synthesis and antimicrobial properties of complexes based on silver and camphor imines. Selected examples of the structure and antimicrobial activity relationships of ligands studied so far are presented, showing the potential of silver camphorimine complexes as novel antimicrobials. The review was published in the journal Antibiotics.

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New Species of the Burkholderia cepacia Complex Isolated from Hospital Settings and Soils

New Species of the Burkholderia cepacia Complex Isolated from Hospital Settings and Soils | iBB | Scoop.it

Bacteria from the Burkholderia cepacia complex (Bcc) can cause severe infections in cystic fibrosis (CF) patients. These opportunistic pathogens are also widely distributed in natural and man-made environments. BSRG researchers Jorge H. Leitão and Sílvia Sousa, together with Carla Carvalho from BERG and an Argentinian research group led by Alejandra Bosch, identified a novel species of the Bcc, named Burkholderia puraquae sp. nov. The work results from a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings. The isolates present a concatenated species-specific allele sequence that differs by more than 3 % from those of the Bcc with validly published names. Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the new species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.

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Antimicrobial Activity of Ag(I) Camphorimine Complexes 

Antimicrobial Activity of Ag(I) Camphorimine Complexes  | iBB | Scoop.it

The synthesis and demonstration of the antifungal/antibacterial activity of three novel [Ag(NO3)L] complexes (named 1, 2 and 3) has recently published in PLOS ONE by BSRG researchers Jorge H leitão and Nuno P. Mira, and M. Fernanda N.N. Carvalho from CQE. The work shows for the first time that previously studied complexes (named 4 to 8) also exert antifungal activity. The antibacterial activity was evaluated against Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia contaminans and Escherichia coli strains, while antifungal activity was tested against the Candida species C. albicans, C. glabrata, C. parapsilosis and C. tropicalis. The antimicrobial activity of the complexes ranged from very high (complex 4) to moderate (complex 6) or low (complex 8), depending on the structural and electronic characteristics of the camphorimine ligands. Notably, the highest antibacterial and anti-Candida activities do not coincide in the same complex and in some cases they were even opposite, as is the case of complex 4 which exhibits a high anti-bacterial and low antifungal activity. These results suggest that the complexes may have different mechanisms against prokaryotic and eukaryotic cells. The antifungal activity of the Ag(I) camphorimine complexes (in particular of complex 1) was found to be very high (MIC = 2 μg/mL) against C. parapsilosis, being also registered a prominent activity against C. tropicalis and C. glabrata. None of the tested compounds inhibited C. albicans growth, being this attributed to the ability of these yeast cells to mediate the formation of less toxic Ag nanoparticles, as confirmed by Scanning Electron Microscopy images. The high antibacterial and anti-Candida activities of the here studied camphorimine complexes, especially of complexes 1 and 7, suggests a potential therapeutic application for these compounds. Click on title to learn more.

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Synthesis, Antimicrobial Activity and Toxicity of Cyclam Derivatives

Synthesis, Antimicrobial Activity and Toxicity of Cyclam Derivatives | iBB | Scoop.it
The emergence of antibiotic resistance in human pathogens has given impetus to the search for entirely new types of antimicrobials. In a paper published in the International Journal of Antimicrobial Agents, a team of researchers led by Jorge H. Leitão from BSRG-iBB and Ana Margarida Martins from CQE-IST evaluated the antimicrobial activity and toxicity to nematodes of the cyclam salt [H2{H2(4-CF3PhCH2)2Cyclam}](CH3COO)2.(CH3COOH)2. Estimated minimum inhibitory concentrations (MICs) of 9, 261 and 15 µg/mL were obtained for Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, respectively. For selected Candida spp., the estimated MICs obtained ranged from 32 µg/mL to 63 µg/mL. Bactericidal activity was demonstrated but the compound was not reliably fungicidal. Concentrations of the cyclam salt up to 32 µg/mL did not significantly affect survival of the nematode Caenorhabditis elegans; however, concentrations equal or above this value significantly affected nematode survival in a dose-dependent manner. Based on the evidence of their antimicrobial activity, cyclam derivatives are potential novel antimicrobials. Click on title to learn more.
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The OmpA-like Protein BCAL2958 is a Potential Candidate for Preventive Therapies of B. cepacia Complex-Infected Cystic Fibrosis Patients

The OmpA-like Protein BCAL2958 is a Potential Candidate for Preventive Therapies of B. cepacia Complex-Infected Cystic Fibrosis Patients | iBB | Scoop.it
Respiratory infections by bacteria of the Burkholderia cepacia complex (Bcc) remain an important cause of morbidity and mortality among cystic fibrosis patients. In a paper recently published in AMB Express, Silvia A. Sousa and Jorge H. Leitão from BSRG-iBB, in collaboration with researchers from Helwan University (Cairo), and medical doctors from Hospitals Sta Maria and D. Estefânia (Lisbon), studied the B. cenocepacia protein BCAL2958, a member of the OmpA-like family of proteins, showing the presence of anti-BCAL2958 antibodies in sera from cystic fibrosis patients with a clinical record of respiratory infection by Bcc, and the ability of the purified protein to in vitro stimulate neutrophils. Results strongly suggest that the protein is a potential candidate for usage in preventive therapies of infections by Bcc.
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André Grilo to Defend PhD Thesis in Biotechnology

André Grilo to Defend PhD Thesis in Biotechnology | iBB | Scoop.it

André Manuel Sardinha Grilo will be defending his Ph D thesis in Biotechnology at Instituto Superior Técnico, wednesday the 17th February 2016 (10:00 H, room PA3). During the last years, and under the supervision of Jorge Leitão from BSRG-IBB, André focused his efforts on the identification of regulatory small non-coding RNAs (sRNAs) from bacteria of the Burkholdeira cepacia complex (Bcc) and on the regulation of gene expression in Bcc bacteria by small non-coding RNAs. The title of Andrés's  thesis is “Identification and characterization of small non-coding RNAs from bacteria of the Burkholderia cepacia complex”. 

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Suitability of a Yeast-based Assay to Assess Pyrimethanil Toxicity in Surface Runoff from Sprayed Soils

Suitability of a Yeast-based Assay to Assess Pyrimethanil Toxicity in Surface Runoff from Sprayed Soils | iBB | Scoop.it

A joint publication from BSRG-iBB and IMAR & MARE – University of Coimbra in the journal Science of the Total Environment describes evaluation of the suitability of a short-term yeast-based gene expression assay to assess the toxicity of pyrimethanil in runoff samples simulated from soils sprayed with fungicide accidental spill doses. Comparison of toxicity data to the yeast and to aquatic and soil standard species pointed the yeast assay relevant for screening worst-cases of fungicide contamination. This work was performed in the context of Fátima Gil’s PhD in Biotechnology (finished july 2014; supervisors: Cristina A Viegas, Jorge H Leitão).

 

Photo sources: en.wikipedia.org, www.zmescience.com, www.marietta.edu, enfo.agt.bme.hu, www.infojardin.com

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