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Characterization of a New Blastobotrys navarrensis Strain

Characterization of a New Blastobotrys navarrensis Strain | iBB | Scoop.it

A BSRG-iBB research paper just published in International Journal of Systematic and Evolutionary Microbiology reports the isolation, identification, and morphological and phylogenetic characterization of Blastobotrys navarrensis IST 508. The isolation of this yeast strain from a soil sample collected underneath an olive tree in Ferreira do Alentejo was essential to propose the taxonomic separation of B. navarrensis, so far represented by the single strain CBS 139.77, from Blastobotrys proliferans, which until this study was considered a synonym species. Molecular and phylogenetic data was used to propose B. navarrensis as an independent species and not a later synonym of B. proliferans. An emended description of B. navarrensis was also proposed. This research paper has as first- and corresponding- author Dr. Margarida Palma, who coordinated the investigation with contributions by Professor Isabel Sá-Correia. The article is also co-authored by former Master students Érica Vieira and Margarida Pataco, both from MSc in Biotechnology, IST.

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Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens

Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens | iBB | Scoop.it

A BSRG-iBB research paper just published in Journal of Fungi (special issue “New Trends in Yeast Genomics”) reports the isolation of Candida membranifaciens strains from soil, their physiological characterization and comparison regarding the production of riboflavin (vitamin B2). The first annotated genome sequence of C. membranifaciens IST 626 is provided, as well as the comparative genomic analysis with other relevant yeast species. This research work is first-authored by Dr. Margarida Palma, who coordinated the investigation with Professor Isabel Sá-Correia. The work has contributions from Master students Mariana Pereira and Érica Vieira and researchers from Joint Genome Institute, Berkeley, USA.

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Cláudia Godinho Delivers Oral Presentations at Microbiotec21

Cláudia Godinho Delivers Oral Presentations at Microbiotec21 | iBB | Scoop.it

Cláudia P. Godinho, a PhD researcher at BSRG-iBB, presented the work "The N.C.Yeastract and CommunityYeastract databases and tools: studying transcriptional regulation in non-conventional yeasts" during the Synthetic & Systems biology Symposium. Cláudia provided a guided exploration of the tools of the N.C.Yeastract database, which gathers all published regulatory associations and TF-binding sites for five yeast species of biotechnological potential. The potential of the database was also explained. The presentation was based in a recent MiniReview published this year in FEMS Yeast Research, resulting from collaborative work between the BSRG-iBB team coordinated by Isabel Sá-Correia and INESC-ID colleagues. Cláudia P. Godinho was also selected for presenting the International Microorganism Day initiative in the Teaching and Communicating in Science symposium. The purpose of the presentation, co-authored by Cláudia and Isabel Sá-Correia, was to report the results from IMD 2021 campaign and event, to promote IMD, and to provide an idea of the power of social media in Microbiology dissemination.

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Exploring the Biological Function of Efflux Pumps for the Development of Superior Industrial Yeasts

Exploring the Biological Function of Efflux Pumps for the Development of Superior Industrial Yeasts | iBB | Scoop.it

The economic competitiveness of yeast-mediated bioprocesses in Chemical Biotechnology requires high tolerance of industrial strains to the multiple stresses that occur. Among the mechanisms used by yeast to overcome those deleterious effects is the activity of plasma membrane transporters involved in multidrug/multixenobiotic resistance (MDR/MXR). A new review article on these still poorly characterized transporters belonging to the MFS and the ABC Superfamily in yeasts of biotechnological relevance has just been published. The reported role of specific transporters in increasing the secretion of metabolites and other added-value bioproducts is also highlighted. The review article is co-authored by the BSRG-iBB researchers Isabel Sá-Correia and Cláudia P. Godinho and was published in the journal Current Opinion in Biotechnology (special Issue: Chemical Biotechnology 2022).

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Exploring N.C.Yeastract to Study Gene and Genomic Regulation in Non Conventional Yeasts

Exploring N.C.Yeastract to Study Gene and Genomic Regulation in Non Conventional Yeasts | iBB | Scoop.it

Responding to the recent interest of the yeast research community in non-Saccharomyces cerevisiae species of biotechnological relevance, the N.C.Yeastract was associated to YEASTRACT+, a curated repository of known regulatory associations between transcription factors (TFs) and target genes in yeasts. A recent Minireview published in FEMS Yeast Research aims to advertise the update of the existing information since the release of N.C.Yeastract in 2019, and to raise awareness in the community about its potential to help the day-to-day work on non-Saccharomyces species, exploring all the information and bioinformatics tools available in YEASTRACT +. Using simple and widely used examples, a guided exploitation is offered. The usage potentialities of the new CommunityYeastract platform by the yeast community are also discussed. The Minireview is coauthored by a BSRG-iBB team coordinated by Isabel Sá-Correia and including Cláudia P. Godinho, Margarida Palma, Miguel C. Teixeira and the PhD students Miguel Antunes and Marta N. Mota, in collaboration with INESC-ID colleagues.

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BSRG-iBB at TVI “A Hora da Verdade” Myth-busting Program

BSRG-iBB at TVI “A Hora da Verdade” Myth-busting Program | iBB | Scoop.it

Cláudia P. Godinho, PhD researcher at Sá-Correia’s lab in BSRG-iBB, participated in the clarification of a well-known myth for the TVI news section “A Hora da Verdade”. The participation can be watched here. The 5-second rule states a time window of 5 seconds where it is safe to pick up food after it has been dropped to the floor. The experimental setup organized at the iBB Biological Sciences Research Group Laboratories contributed to demonstrate that the transfer of bacteria from a contaminated floor to food takes less than 5 seconds to happen and that the “rule” is in fact false.

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Adaptation to Acetic Acid Stress Involves Structural Alterations and Increased Stiffness of the Yeast Cell Wall

Adaptation to Acetic Acid Stress Involves Structural Alterations and Increased Stiffness of the Yeast Cell Wall | iBB | Scoop.it

The role of the cell wall in yeast response and tolerance to stress is frequently neglected. A BSRG-iBB research paper just published in Scientific Reports, provides, for the first time, a comprehensive view of the alterations occurring at the cell wall in a yeast population adapting to sub-lethal stress induced by acetic acid. The results reveal changes to the cell wall polysaccharide composition and nanomechanical properties, as well as alterations in the transcript levels of key cell wall biosynthetic genes. This paper reinforces the notion that the adaptive yeast response to acetic acid involves coordinated alterations of the cell wall at the biophysical and molecular levels. The gathered knowledge is important for the design of superior industrial strains and for the efficient control of the deleterious activity of spoilage yeasts, particularly in the Food Industry. This research work is first-authored by the PhD student of the PhD programme in Biotechnology and Biosciences Ricardo Ribeiro (FCT_DP AEM fellowship), performed under the supervision of Isabel Sá-Correia. This collaborative study with Fábio Fernandes (BSIRG-iBB) and Mário S. Rodrigues and his team (BioISI, Faculty of Sciences, ULisboa), is also coauthored by Cláudia Godinho (posdoc researcher) and the PhD student Nuno Bourbon-Melo (FCT_DP BIOTECnico) from the BSRG-iBB team.

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Special Issue “Yeast Biorefineries”

Special Issue “Yeast Biorefineries” | iBB | Scoop.it

The Special Issue “Yeast Biorefineries” edited by Isabel Sa-Correia and Naseem A. Gaur for the Journal of Fungi intends to gather the most recent and original research and review papers on the identification, development, and exploitation of robust, metabolically promising, and genetically engineered yeast strains for advanced biorefineries. Saccharomyces cerevisiae is a major cell factory for which vast biological knowledge and genetic tools are available. However, the exploitation of non-Saccharomyces yeasts is recently gaining momentum, making this large and heterogeneous group desirable for the synthesis of a wide range of added-value products, in particular lipids/oleofuels, bioethanol, carotenoids, organic acids, and biopolymers. Papers on the identification, exploitation, and development of robust, metabolically promising, and genetically engineered conventional and non-conventional yeast strains for biorefineries are welcome. Contributions that explore functional and comparative genomics analyses for understanding the physiological genomics of yeasts and the development of bioprocesses, especially those dedicated to the valorization of bioresidues and production of added-value products are also welcome.

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Six iBB Researchers are Among the Most Cited Top Scientists in the World

Six iBB Researchers are Among the Most Cited Top Scientists in the World | iBB | Scoop.it

Six iBB researchers are among the most cited top scientists at the top 2% of their areas. Portugal is represented in this list compiled by Stanford University with 385 scientists affiliated to national institutions. The rankings are based upon a researcher’s citations for both a single year (2019) and cumulative across their careers. Titled “Updated science-wide author databases of standardized citation indicators,” the list uses algorithms that quantify and systematically rank individuals into consistent scientific fields. The scores are provided  with and without self-citations to lessen the impact of self-citations or the use of citation farms (small clusters of researchers massively citing each other’s work). Ranked within the careerlong citation impact list are these iBB researchers: Joaquim Cabral, Isabel Sá-Correia, Mário Berberan Santos, Miguel Prazeres, Carla Carvalho, also along with Carina Crucho in the
2017 single-year citations list.

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Use of Non-conventional Yeasts to Enrich the Aroma Profile of Beer

Use of Non-conventional Yeasts to Enrich the Aroma Profile of Beer | iBB | Scoop.it

There is a wide diversity of non-conventional yeast species, which often present distinctive metabolic properties and can therefore impart different flavours and aromas to beer. Because of this, brewers now regard non-conventional yeast species as a tool to differentiate their beers from other products in the market. In fact, some of these yeasts are already used to produce specific beer styles, such as lambics. A study recently published in Food Microbiology, coordinated by Dr. Margarida Palma and Prof. Isabel Sá-Correia and first authored by the BIOTECnico PhD student Nuno Melo, investigates how two non-conventional yeast species commonly found in wineries, Hanseniaspora guilliermondii and Hanseniaspora opuntiae, influence the volatile composition of beer. These two species, initially selected by a preliminary organoleptic evaluation, were found to modulate the aroma profile of beer, namely by increasing phenylethyl acetate concentration (‘rose’, ‘honey’ aroma). These findings highlight the importance of non-conventional yeasts in shaping the aroma profile of beer and suggest a role for Hanseniaspora spp. in improving it.

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The ABC Transporter Pdr18 is Required for Yeast Thermotolerance

The ABC Transporter Pdr18 is Required for Yeast Thermotolerance | iBB | Scoop.it

A new paper by researchers from iBB-BSRG describes, for the first time, the involvement of a plasma membrane multidrug/multixenobiotic resistance (MDR/MXR) ABC transporter in yeast thermotolerance. The expression of the ABC transporter Pdr18, required for pleotropic drug resistance and involved in ergosterol transport at the plasma membrane level, is related with decreased plasma membrane permeabilization induced by a supraoptimal growth temperature. Ergosterol biosynthesis and plasma membrane ergosterol content were implicated in yeast response and capacity to overcome stress induced by supraoptimal growth temperatures. These results support the notion that MDR/MXR transporters perform biological activities other than their accepted role as drug/xenobiotic exporters. Cláudia Godinho is the first author of this research work, coordinated by Isabel Sá-Correia and published in the journal Environmental Microbiology.

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Valorisation of Pectin-Rich Agro-Industrial Residues by Yeasts: Potential and Challenges

Valorisation of Pectin-Rich Agro-Industrial Residues by Yeasts: Potential and Challenges | iBB | Scoop.it

Pectin-rich agro-industrial residues generated worldwide from the industrial processing of fruits and vegetables,are feedstocks with potential for sustainable biorefineries. Although the most important yeast cell factory platform is Saccharomyces cerevisiae, this species cannot naturally catabolise the main sugars present in pectin-rich agro-industrial hydrolysates, in particular D-galacturonic acid and L-arabinose. However, there are non-conventional yeasts considered advantageous alternatives whenever they can express highly interesting metabolic pathways, natively assimilate a wider range of carbon sources or exhibit higher tolerance to relevant bioprocess-related stresses. For this reason, the interest in nonconventional yeasts is gaining momentum. A review published in Applied Microbiology and Biotechnology discusses major challenges and progress on the isolation, selection, sugar catabolism, metabolic engineering and use of nonconventional yeasts and S. cerevisiae-derived strains for the bioconversion of pectin-rich residue hydrolysates  Examples of value-added products synthesised by different yeasts using pectin-rich residues are also reviewed. The first author of this paper is the PhD student of the FCT Doctoral Program in Applied and Environmental Microbiology Luís C. Martins advised by Prof. Isabel Sá-Correia from iBB-BSRG.

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Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products

Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products | iBB | Scoop.it

Pharmaceutical products contaminated with bacteria of the Burkholderia cepacia complex (Bcc) constitute a serious risk for patient ssffering from cystic fibrosis and Chronic granulomatous disease. Bcc infection also has been reported in immunocompromised individuals (e.g., cancer patients submitted to chemotherapy, HIV/AIDS patients, mechanically ventilated patients, and infants/the elderly). Over recent years, both sterile and nonsterile pharmaceutical products have been recalled from the market due to Bcc contamination and subsequent nosocomial outbreaks. In a review paper just published in Clinical Microbiology Reviews, the role of Bcc bacteria as a potential public health problem, the mechanisms behind their success as contaminants of pharmaceutical products, particularly in the presence of biocides, the difficulties encountered in their detection, and the preventive measures applied during manufacturing processes to control contamination with these objectionable microorganisms  are addressed. A summary of Bcc-related outbreaks in different clinical settings, due to contamination of diverse types of pharmaceutical products, is provided. The corresponding author Is Isabel Sá-Correia from iBB/BSRG and the first author is a recent graduate of the MSc in Biotechnology.

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Special Issue on "Yeasts for a Sustainable Circular Bio-Based Economy" for The Journal of Fungi

Special Issue on "Yeasts for a Sustainable Circular Bio-Based Economy" for The Journal of Fungi | iBB | Scoop.it

Together with Dr. Naseem A. Gaur, prof. Isabel Sá-Correia is organizing a special issue for The Journal of Fungi. This special issue is focused on the new circular bio-based economy. The development of large-scale bioprocesses for the bioconversion of biomass resources into environmentally friendly biofuels, chemicals, and materials is required to answer timeless societal challenges. Cost-effective microbial conversion processes of renewable feedstock into biofuels and biochemicals are of utmost importance for the establishment of a robust bioeconomy. Therefore, the transition to a sustainable bio-based economy requires further scientific research and innovation to reach those goals. Integrated yeast biorefinery processes have recently gained relevance in envisaging the conversion of forest and agro-industrial residues into environmentally friendly products currently produced by the petrochemical industry. For all these reasons, this is a very active and exciting area of R&D. Deadline for new manuscript submissions is August 31, 2022.

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Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness

Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness | iBB | Scoop.it

A BSRG-iBB research paper just published in Journal of Fungi (special issue “Yeast Biorefineries”) provides a comprehensive view of the effect that the content of ergosterol at yeast plasma membrane, associated to membrane ABC transporter Pdr18 activity, influences cell wall biophysical properties under acetic acid-induced stress,  as part of a coordinated response to counteract the deleterious effects of this important stress factor in lignocellulosic biorefineries. This research work is first-authored by the PhD student of the PhD programme in Biotechnology and Biosciences Ricardo Ribeiro (FCT_DP AEM fellowship), performed under the supervision of Isabel Sá-Correia and is also co-authored by Cláudia P. Godinho, also from the BSRG-iBB team. This is a collaborative study with Fábio Fernandes (BSIRG-iBB) and Mário S. Rodrigues and his team (BioISI, Faculty of Sciences, ULisboa).

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Marta Mota Delivers Oral Presentation at Microbiotec’21

Marta Mota Delivers Oral Presentation at Microbiotec’21 | iBB | Scoop.it

Marta Mota, a PhD student of the Instituto Superior Técnico, Universidade de Lisboa, PhD program in Biotechnology and Biosciences, (FCT doctoral programme in Applied and Environmental Microbiology (DP_AEM)), presented her work during the symposium on “Microbiotech for a Sustainable Future” of Microbiotec’ 21 Congress taking place online from 23-26 November. The presentation was entitled “Promising Genetic Determinants of Methanol Tolerance for Yeast Robustness Engineering for Biorefineries”. This work was performed in the Biological Sciences Research Group of iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, under Professor Isabel Sá-Correia’s supervision.

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Metabolic Engineering of Saccharomyces cerevisiae Towards the Valorization of Pectin-rich Residues

Metabolic Engineering of Saccharomyces cerevisiae Towards the Valorization of Pectin-rich Residues | iBB | Scoop.it

A recent article published in the journal Metabolic Engineering reports the metabolic engineering of the yeast Saccharomyces cerevisiae envisaging the fast and complete consumption of D-galacturonic acid (d-GalUA) in crude sugar beet pulp hydrolysate under aerobic conditions. To this end, the fungal d-GalUA pathway was inserted in an S cerevisiae strain equipped with an NAD-dependent glycerol catabolic pathway. The constructed strain was able to consume d-GalUA with the highest reported maximum specific rate in synthetic minimal medium when glycerol was added. This work is the result of joint research in the framework of project YEASTPEC (Engineering of the yeast Saccharomyces cerevisiae for bioconversion of pectin-containing agro-industrial side-streams), funded through the ERA-IB scheme. In Portugal, the project was led by Isabel Sá-Correia (BSRG/iBB), in Germany by Elke Nevoigt, Jacobs University (corresponding author) and Wolfgang Liebl, TUM, and at VTT, Finland, by Peter Richard. The BSRG/iBB PhD graduate in Biotechnology and Biosciences Luis Martins also co-authored this article.

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Prediction of the Haa1-regulatory Network in the Oleaginous Yeast Rhodotorula toruloides

Prediction of the Haa1-regulatory Network in the Oleaginous Yeast Rhodotorula toruloides | iBB | Scoop.it

A new bioinformatics tool, developed by our computational biologist collaborators at INESC-ID, is described in a recent article published in BMC Bioinformatics. The BSRG-iBB team contributed to the application of the tool to predict the putative Haa1-regulon in the lipid producing yeast cell factory Rhodotorula toruloides. For this, genomic information on this species was collected and included in the CommunityYeastract to benefit from the access to all the comparative genomics queries offered in the YEASTRACT + portal. The transcription factor Haa1 is the main regulator of S. cerevisiae genomic response to acetic acid stress, regulating, directly or indirectly, the majority of acetic acid tolerance genes. The prediction of RtHaa1-regulon has an expected impact in the optimization of R. toruloides robustness for biorefinery processes using hydrolysates from lignocellulosic residues. The BSRG-iBB team involved in these studies included the PhD student Miguel Antunes, his supervisor Isabel Sá-Correia, and Cláudia P. Godinho and Miguel C. Teixeira. 

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Nuno Melo Participates in World Microbe Forum

Nuno Melo Participates in World Microbe Forum | iBB | Scoop.it

We owe a great debt to yeasts for their contributions to humankind. In the Food Industry alone, yeasts play a central role in breadmaking, winemaking, brewing, and more. The fact that yeasts present such spectacular diversity constitutes an opportunity to use different, unused yeasts to obtain fermented goods with distinct properties and organoleptic profiles. In his interactive poster submission to the international conference World Microbe Forum, a joint initiative by the American Society for Microbiology (ASM) and Federation of European Microbiological Sciences (FEMS) taking place online from 20-24 June 2021, Nuno Bourbon-Melo talks about the role of non-conventional yeasts seldom used in brewing, specifically Hanseniaspora opuntiae and H. guilliermondii, to improve the aroma and flavour of beer. Learn more about it by clicking here. The work was performed at iBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, ULisboa, and advised by Prof. Isabel Sá-Correia and Dr. Margarida Palma.

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Luís Martins to Defend PhD Thesis in Biotechnology and Biosciences

Luís Martins to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Luís Miguel Candeias Martins will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, Tuesday the 18th of May 2021, at 14:00 (https://videoconf-colibri.zoom.us/j/82200706553). During the last years, and under the supervision of Prof. Isabel Sá-Correia from BSRG-iBB, Luís studied the genetic tolerance determinants for multiple stresses in S. cerevisiae and the potential of oleaginous red yeasts for the efficient carbon substrates conversion present in pectin-rich hydrolysates. The title of his thesis is “Valorisation of pectin-rich agro-industrial residues by yeasts: Potential and Challenges”.

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Exploring Oleaginous Red Yeasts for Sugar Beet Pulp Valorization

Exploring Oleaginous Red Yeasts for Sugar Beet Pulp Valorization | iBB | Scoop.it

Agro-industrial residues rich in pectin are generated in high amounts worldwide from the sugar industry or the industrial processing of fruits and vegetables. A recent paper by BSRG-iBB researchers, published in the Journal of Fungi (special issue “Yeast Biorefineries” (edited by Isabel Sá-Correia and Naseem Gaur), describes the optimization of the performance of Rhodotorula strains envisaging the use of the major carbon sources present in Sugar Beet Pulp (SBP) hydrolysates. SBP valorization through the production of lipids and carotenoids by the oleaginous red yeasts examined, supported by complete catabolism of the major carbon sources present, looks promising for industrial implementation. The work was conducted by the PhD student of the IST PhD Programme in Biotechnology and Biosciences Luís C. Martins (FCT_DP AEM programme fellowship) under the supervision of Isabel Sá-Correia. This work was developed in the context of the ERA-NET-Industrial Biotechnology-2 project, “YEASTPEC-Engineering of the yeast Saccharomyces cerevisiae for bioconversion of pectin-containing agro-industrial side-streams”.

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Genetic Determinants of Methanol Tolerance for Robustness Engineering of Yeasts

Genetic Determinants of Methanol Tolerance for Robustness Engineering of Yeasts | iBB | Scoop.it

Methanol is a promising feedstock for metabolically competent yeast strains-based biorefineries. However, methanol toxicity can limit the productivity of these bioprocesses. For this reason, the identification of genes whose expression is required for maximum methanol tolerance is important for mechanistic insights and rational genomic manipulation to obtain more robust methylotrophic yeast strains. The chemogenomic analysis performed in this new BSRG-iBB study provided new valuable information on genes and potential regulatory networks involved in overcoming methanol toxicity in the model yeast Saccharomyces cerevisiae. The article was just published in the Journal of Fungi and belongs to the Special Issue “Yeast Biorefineries” (edited by Isabel Sá-Correia and Naseem A. Gaur). The article has as co-authors the PhD students of the IST Biotechnology and Biosciences PhD Programme ( FCT_DP Applied and Environmental Microbiology) Marta N. Mota (first  author) and Luís C. Martins, under Prof. Isabel Sá-Correia’s supervision. The gathered knowledge is an important starting point for the improvement of methanol tolerance in yeasts capable of catabolizing and copying with methanol concentrations present in promising bioeconomy feedstocks, including industrial residues.

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Comparative Evolutionary Patterns of Burkholderia cenocepacia and B. multivorans During Chronic Co-infection of a Cystic Fibrosis Patient Lung

Comparative Evolutionary Patterns of Burkholderia cenocepacia and B. multivorans During Chronic Co-infection of a Cystic Fibrosis Patient Lung | iBB | Scoop.it

During chronic respiratory infections of cystic fibrosis (CF) patients, bacteria adaptively evolve in response to the nutritional and immune environment. A research paper from iBB-BSRG researchers, just published in Frontiers in Microbiology (part of the Research Topic: Evolutionary Mechanisms of Infectious Diseases), contributes to the understanding of shared and species specific evolutionary patterns of B. cenocepacia and B. multivorans evolving in the same CF lung environment. Results support the idea that positive selection might be driven by the action of the host immune system, antibiotic therapy and low oxygen and iron concentrations. This research work, coordinated by Prof. Isabel Sá-Correia, results from the PhD thesis in Biotechnology and Biosciences (BIOTECnico program) of A. Amir Hassan. Dr. AA Hassan is currently working at the Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Belgium.

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Adaptation and Survival Strategies of Bacteria in Aqueous Pharmaceutical Products

Adaptation and Survival Strategies of Bacteria in Aqueous Pharmaceutical Products | iBB | Scoop.it

The Burkholderia cepacia complex (Bcc) bacteria are feared contaminants in pharmaceutical industries and cause nosocomial outbreaks, posing health threats to immunocompromised individuals and cystic fibrosis (CF) patients. In this study, the adaptation and survival of B. cepacia and B. contaminans isolates was investigated after long-term incubation in nutrient depleted saline solutions supplemented with increasing concentrations of the biocidal preservative benzalkonium chloride (BZK), recreating the storage conditions of pharmaceutical products. This study just published in the section Biosafety and Biosecurity of Frontiers in Bioengineering and Biotechnology is co-authored by several members of Isabel Sá-Correia team from iBB/BSRG. This study reveals mechanisms underlying the prevalence of Bcc bacteria as contaminants of aqueous pharmaceutical products containing BZK, which often lead to false-negative results during quality control and routine testing.

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Rui Pacheco Defended PhD Thesis in Biotechnology and Biosciences

Rui Pacheco Defended PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Rui Cruz Pacheco defended his PhD thesis in Biotechnology and Biosciences on the 15th of April, 2020. The thesis, entitled “Production of first-and second-generation bioethanol by yeasts: global warming potential and fermentation productivity issues” was supervised by Carla Silva, Faculdade de Ciências da Universidade de Lisboa and Instituto Dom Luiz and co-supervised by Isabel Sá-Correia from BSRG-iBB. Rui prepared part of his PhD thesis in the laboratories of the BSRG examining factors that limit first-and second-generation fermentation productivity and improving industrial yeasts performance through genetic manipulation. Most of his work was dedicated to the Life Cycle Assessment (LCA) of bio-based ethanol production.

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