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Institute for Bioengineering and Biosciences
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Optimizing the Performance of Chromatographic Separations Using Microfluidics

Optimizing the Performance of Chromatographic Separations Using Microfluidics | iBB | Scoop.it

The establishment of novel, simpler and improved purification processes is extremely important to make antibody-based treatment options more accessible and with a lower economic burden to healthcare systems and patients. In this context, the development of microfluidic tools for the optimization of chromatography operating conditions can offer advantages such as low consumption of molecules, fast generation of results and ability to automate and multiplex the assays. In a collaboration between INESC-MN and iBB, researchers have recently reported a novel microfluidics-based methodology to perform rapid and multiplexed screening of various chromatography ligands relative to their ability to bind different target molecules in solution. The work was published in the Biotechnology Journal and is expected to contribute to the fields of separation sciences and analytical chemistry and inspire new developments within the purification of biopharmaceuticals or other molecules.

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Inês Pinto Defends PhD Thesis in Biotechnology and Biosciences

Inês Pinto Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Inês Pinto will be defending her PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, monday the 24th September 2018 (9:30 H, room PA3). During the last years, and under the supervision of Profs. Ana Azevedo and Raquel Barros from BERG-iBB, and João Pedro Conde from INESC-MN, Inês developed a microfluidic platform for the rapid screening of the chromatographic purification of monoclonal antibodies. The title of her thesis is "Screening of multimodal ligands for the capture and polishing of antibody streams: A microfluidic approach for rapid optimization of chromatographic operating conditions”.

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Quantitative Analysis of Bead-Based Microfluidic Optical Biosensors

Quantitative Analysis of Bead-Based Microfluidic Optical Biosensors | iBB | Scoop.it

Microfluidic devices with integrated photodetectors can address the need for compact and portable biosensing platforms for medical, veterinary, environmental and food safety applications. However, depending on the application, performance requirements like the minimum detectable limits, the target sensitivity, and the detection time can vary dramatically. To improve the engineering of fit-for-purpose bead-based microfluidic biosensors, researchers at BERG-iBB and INESC-MN developed a simple quantitative methodology to determine (1) equilibrium constants, (2) binding kinetics, (3) rate at which photons are generated/absorbed per captured molecule, and (4) molecular capture efficiencies. These parameters provide an effective trade-off between required sensitivity, cost effectiveness and assay complexity. This work was published in the IOP Journal of Micromechanics and Microengineering.

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Talk on Miniaturization of Chromatography by Inês Pinto at the 10th HIC/RPC Hydrophobic Bioprocessing Conference

Talk on Miniaturization of Chromatography by Inês Pinto at the 10th HIC/RPC Hydrophobic Bioprocessing Conference | iBB | Scoop.it
Inês Pinto from BERG-iBB is giving a talk entitled "Miniaturization of chromatography for rapid optimization of antibodies separations: multiplexed screening of different ligands and target molecules." at the HIC/RPC Hydrophobic Bioprocessing Conference, held in Scottsdale, Arizona/USA (13th-16th February). The Conference is focused on the hydrophobic nature of biological targets and the science related to their isolation and purification by chromatography and other modes of separation. Click on title to learn more.
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iBB-BERG Represented at ISPPP 2016

iBB-BERG Represented at ISPPP 2016 | iBB | Scoop.it
iBB was recently represented at the International Symposium ISPPP 2016 by researchers from BERG's Bioseparation Engineering Lab, who presented 3 oral communications (Raquel Aires-Barrros, Ana Azevedo and Emanuel Capela) and 2 poster communications (Sara Rosa and Inês Pinto).
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Purification Technologies for Monoclonal Antibodies

Purification Technologies for Monoclonal Antibodies | iBB | Scoop.it

The commercial potential of monoclonal antibodies (mAbs) has been continuously increasing during the last years alongside with the number of approved mAb-based drugs and clinical trials. Despite their effectiveness and safety, the general access to this class of biopharmaceuticals is barred by high selling prices. Downstream processing is now considered the bottleneck in the manufacturing of mAbs. Therefore, the design of novel and economic operations and their implementation in the current technology platforms constitutes a pressing need. A recent review published by researchers from the Bioseparation Engineering Laboratory at BERG-iBB, provides an insight into the current state-of-the-art in mAbs purification, focusing on multimodal chromatography as one of the viable options to upgrade the established purification train. Multimodal chromatography has been receiving considerable attention over the last years, with about 152 publications published in ISI-index journals in the last decade (2004-2013). The number of publications per year has been steadily increasing, with a paper-boom starting in 2008.

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Bead-based Microfluidic Platform for DNA Hybridization

Bead-based Microfluidic Platform for DNA Hybridization | iBB | Scoop.it

The demand for portable, rapid, sensitive and low-cost DNA detection systems that could be used for rapid diagnostic of disease and infection has motivated the development of new DNA detection platforms in recent years. BERG-iBB researchers working with colleagues from INESC-MN report a rapid and simple bead-based microfluidic platform to detect a specific 22-mer DNA sequence via hybridization, serving as a model of a miRNA biomarker for tumor and cardiovascular diseases. Nanoporous chromatography beads were used as solid support for probe DNA immobilization. The lowest limit of detection of 9.5 pM was achieved with an assay time of 10 min using a quantum dot label coupled with a multi-mode immobilization. The results highlight the potential of the system as a simple DNA hybridization platform, that achieves low pM sensitivities without the need for DNA amplification. The work was published in Sensors and Actuators B.

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Inês Pinto Wins ESBES 2018 Award

Inês Pinto Wins ESBES 2018 Award | iBB | Scoop.it

Inês Pinto from BERG-iBB just won the ESBES Award at the ESBES 2018 conference held in Lisbon (8-12 September 2018) for her work “Optimizing the performance of chromatographic separations using integrated microfluidic devices: adsorption/elution kinetic studies and multiplexed analysis”. The work is part of Inês' PhD project on Biotechnology and is co-authored by Raquel Aires Barros, Viginia Chu, João Pedro Conde and Ana Azevedo. Diogo Pinto from SCERG was one of the runnerups of the competition. The ESBES Award is granted biannually by the European Society of Biochemical Engineering Sciences and aims at recognizing outstanding PhD thesis or scientific publications by students or early carrier researchers in the field of biochemical engineering.

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Chromatography-on-a-Chip

Chromatography-on-a-Chip | iBB | Scoop.it

Optimization of chromatography is a lengthy and demanding task that requires large volumes of reagents and costly equipment. Ana Azevedo and colleagues from BERG-iBB, in collaboration with João Pedro Conde from INESC-MN, describe an integrated, reusable and portable microfluidic platform for the rapid screening of chromatographic conditions. The platform was tested for the capture of fluorophore-labeled monoclonal antibodies from cell culture supernatants using a multimodal ligand (Capto MMC). The work is part of Inês Pinto´s PhD thesis in Biotechnology and Biosciences. Click on title to leran more in the journal Sensors and Actuators B.

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Inês Pinto Receives Best Poster Award at ISPPP 2016

Inês Pinto Receives Best Poster Award at ISPPP 2016 | iBB | Scoop.it
Inês Pinto has received the 1st best poster award at the International Symposium ISPPP 2016 for her communication entitled "On chip chromatography for rapid screening of multimodal ligand-target interactions". This project arises from a collaboration between Ana Azevedo from BERG-iBB and João Pedro Conde from INESC-MN. Inês is currently in her 3rd year of doctoral studies in Biotechnology and Biosciences at Instituto Superior Técnico. Congratulations Inês!
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High-Throughput Nanoscale Chromatography

High-Throughput Nanoscale Chromatography | iBB | Scoop.it
Multimodal chromatography is based on the use of synthetic ligands that provide more than one type of interaction with target analytes in a mobile phase. This technique has been showing a great potential for the purification of different biopharmaceuticals within complex biological mixtures, particularly monoclonal antibodies (mAbs). However, multimodal chromatography requires extensive optimization in a case-by-case basis to address specific purification problems. In a recent publication in the prestigious journal Analytical Chemisrty, researchers from BERG-IBB and INESC-MN led by Ana Azevedo and João Pedro Conde, developed a microfluidic platform to expedite optimization of adsorption and elution conditions of mAbs to a multimodal ligand using low amounts of reagent in a rapid and simple manner. Click on title to learn more.
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