iBB
46.4K views | +5 today
Follow
iBB
Institute for Bioengineering and Biosciences
Curated by iBB
Your new post is loading...
Your new post is loading...
Scoop.it!

Transcriptomic Profiling of Human Pluripotent Stem Cell-Derived Cerebellar Organoids

Transcriptomic Profiling of Human Pluripotent Stem Cell-Derived Cerebellar Organoids | iBB | Scoop.it

 

Endogenous human brain tissue is not easily available for studying neurodevelopment and neurodegenerative diseases. However, human pluripotent stem cells (PSCs) have been used to generate a variety of glial and neuronal cells of the central nervous system. Still, reproducible protocols for generating in vitro models of the human cerebellum are scarce. In this context, Silva et al. describe the scalable production of human PSC-derived cerebellar organoids using single-use vertical-wheel bioreactors. The transcriptomic profile of cerebellar organoids derived under dynamic conditions demonstrates a faster cerebellar differentiation combined with significant enrichment of extracellular matrix and upregulation of transcripts involved in angiogenesis when compared with the static protocol. The authors anticipate that large-scale production of cerebellar organoids may help developing models for drug screening, toxicological tests and studying pathological pathways involved in cerebellar degeneration.

No comment yet.
Scoop.it!

Teresa Silva Defends PhD Thesis in Bioengineering (Cell Therapies and Regenerative Medicine)

Teresa Silva Defends PhD Thesis in Bioengineering (Cell Therapies and Regenerative Medicine) | iBB | Scoop.it

Teresa Silva will be defending his PhD thesis in Bioengineering (MIT Portugal) at Instituto Superior Técnico, wednesday the 26th October 2018 (15:00 H, room PA3). During the last years, and under the supervision of Carmo Fonseca from IMM and Tiago Fernandes and Evguenia Bekman from SCERG-iBB, Teresa worked on the development of new models to study neurodegenerative diseases such as ataxias that are caused by dysfunction of the cerebellum. The title of her thesis is "Novel bioengineering strategies for modeling cerebellar ataxias”.

No comment yet.
Scoop.it!

iBB Awarded with Grant to Explore Therapeutic Strategies for Angelman Syndrome

iBB Awarded with Grant to Explore Therapeutic Strategies for Angelman Syndrome | iBB | Scoop.it

Evguenia Bekman and Tiago Fernandes from SCERG-iBB together with Simão Teixeira da Rocha from IMM-FMUL have been awarded by the Amélia de Mello Foundation (FAM) with the Pedro Maria José de Mello Costa Duarte Scholarship to carry out the project “Exploring new therapeutic strategies for Angelman syndrome: A disease modeling approach based on 3D cerebellar organoids derived from patient iPSCs”. The Angelman Syndrome is a genetic-neurological disease discovered in the 60s of the last century by the English pediatrician Harry Angelman, which is estimated to affect 1 in every 15,000 live births and which is characterized by developmental delay, difficulty in speech, sleep disorders, convulsions, disconnected movements and a frequent smile.

No comment yet.