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Institute for Bioengineering and Biosciences
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Benzyl Alcohol Production in Stirred and Packed Bed Reactors

Benzyl Alcohol Production in Stirred and Packed Bed Reactors | iBB | Scoop.it

The ocean is an excellent source for new biocatalysts due to the tremendous genetic diversity of marine microorganisms, and it may contribute to the development of sustainable industrial processes. In a recent paper published in Microorganisms, Carlos J.C. Rodrigues and Carla C.C.R. de Carvalho (iBB-BERG) used a marine bacterium for the conversion of benzaldehyde to benzyl alcohol, which is an important chemical employed as a precursor for producing esters for cosmetics and other industries. A stirred reactor, using a fed-batch approach, enabled a 1.5-fold increase in benzyl alcohol productivity when compared with batch mode. However, product accumulation in the reactor hindered the conversion. The use of a continuous flow reactor packed with immobilized cells enabled a 9.5-fold increase in productivity when compared with the fed-batch stirred reactor system.

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Participation in the EFB2021

Participation in the EFB2021 | iBB | Scoop.it

Carlos JC Rodrigues and Carla CCR de Carvalho (iBB-BERG) presented their work in EFB2021, the major scientific conference organised by the European Federation of Biotechnology. The two studies presented focused on the marine environment as a source of biocatalysts for suitable bioprocesses, and on using microbial heterogeneity to improve bioprocesses. The virtual conference took place during May 10th-14th.

EarpieceHub's comment, June 7, 2021 8:11 AM
nicew
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Carlos Rodrigues Presents his Work at Amine Biocatalysis 4.0

Carlos Rodrigues Presents his Work at Amine Biocatalysis 4.0 | iBB | Scoop.it

The Ph.D. student Carlos Rodrigues, of the Ph.D. program in Biotechnology and Biosciences (BIOTecnico), presented a poster at the Amine Biocatalysis 4.0 conference in Stuttgart, Germany. The poster was about the production of a trifluoromethylamine using a transaminase from a marine bacteria. Mono and biphasic systems, containing organic solvents as substrate reservoirs, were used and the performance of the biocatalyst was evaluated by proper kinetic parameters. The work was carried out under the supervision of Prof. Carla CCR de Carvalho (iBB-BERG), with the collaboration of Dr. Manuel Ferrer (CSIC, Spain).

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Microbial Degradation of Paraffin Wax

Microbial Degradation of Paraffin Wax | iBB | Scoop.it

During crude oil extraction, the reduction in temperature and pressure results in the precipitation of paraffin wax, which may accumulate inside production tubes, pipelines, and also in tankers during petroleum transportation. Few bacterial strains are able to degrade this type of solid substrates. In a paper, published  in Biotechnology Journal, Carlos Rodrigues and Carla CCR de Carvalho (BERG-iBB) evaluate the biodegradation of paraffin by Rhodococcus erythropolis cells. The cells could grow as a thick biofilm over the solid substrate and rapidly degrade paraffin when it was supplied in the form of microparticles. The phenotypic adaptations allowing the cells to degrade the 20-40 chain hydrocarbons are discussed in the paper.

Aptech Visa's comment, July 9, 2019 8:35 AM
Saskatchewan Express Entry - https://www.aptechvisa.com/sinp-saskatchewan-express-entry
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Participation in the EU Project FuturEnzyme

Participation in the EU Project FuturEnzyme | iBB | Scoop.it

A team of researchers from iBB-BERG, including Carlos Rodrigues, Ricardo Pereira, Pedro Fernandes, Prof. Joaquim Sampaio Cabral and led by Carla de Carvalho, will contribute to the development of enzymes for a new generation of environmentally friendly consumer products in the EU project FuturEnzyme. The consortium of 16 leading academic and industrial partners, led by Dr. Manuel Ferrer from CSIC (Spain), will develop innovative solutions that will combine big biodata mining and bio-prospecting, disruptive machine learning and protein engineering, nano-biotechnology, fermentation and downstream-processing systems, and pre-industrial testing. Innovative solutions will be proposed to discover, design, optimize and formulate new enzymes to develop detergents, textiles and cosmetics that combine a high level of functionality with improved sustainability, thus responding to the demands from consumers and industries.

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Asymmetric Synthesis with a Marine Transaminase

Asymmetric Synthesis with a Marine Transaminase | iBB | Scoop.it

w-Transaminases (w-TA) have a pivotal role in the synthesis of chiral amines used as building blocks for drugs and pharmaceuticals. In a recently published paper, Carlos JC Rodrigues (iBB-BERG), Manuel Ferrer (CSIC, Spain) and Carla CCR de Carvalho (iBB-BERG) used a w-TA from a marine bacterium to synthesize the fluorine chiral amine (S)-1-(4-trifluoromethylphenyl)ethylamine from a bulky ketone. An in situ product removal (ISPR) approach using an aqueous organic two-phase system was used to mitigate product inhibition, and engineering tools were used to increase substrate mass transfer without affecting the ability of the organic phase to prevent inhibition of the enzyme activity. The insights gained during process development may be valuable for future process applications of TA reactions.

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Transaminase Activity by Time-lapse Imaging

Transaminase Activity by Time-lapse Imaging | iBB | Scoop.it

The development of bioprocesses for industrial applications is often hampered by the use of time-consuming systems needed to find and characterize new biocatalysts. In particular, transaminases for the synthesis of optically pure chiral amines usually require multi-enzymatic and/or expensive high throughput equipment. In a recent published paper, Carlos JC Rodrigues (BERG-iBB), João M Sanches (LARSyS, ISR), and Carla CCR de Carvalho (BERG-iBB), developed an automatic time-lapse imaging system allowing both the identification of the most promising transaminases, and the determination of their kinetic parameters. The developed method reduces the time required for detection and quantification of specific transaminase activity, thus contributing to the success of biocatalyst application in industrial processes.

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