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Institute for Bioengineering and Biosciences
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May 3, 11:14 AM
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Research visit of Norce colleagues

Research visit of Norce colleagues | iBB | Scoop.it
Carla C.C.R. de Carvalho and Sebastião Tavares welcomed colleagues from Norce, Norway, in the laboratory during a week in April. During the stay, they worked together in research activities related to the project Yum Algae. In this project, both teams work to use enzymes to improve the organoleptic and sensory properties of microalgae for food products.
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Research stay at Norce

Research stay at Norce | iBB | Scoop.it

Carla C.C.R. de Carvalho and Sebastião Tavares did a one-week research stay at Norce, in Bergen, Norway. During the research stay, they worked with the Norwegian team of the project Yum Algae. In this project, both teams work to use enzymes to improve the organoleptic and sensory properties of microalgae for food products. During the stay, Carla and Sebastião had the opportunity to test several techniques, from enzyme purification to harvesting microalgae produced in large photobioreactors.

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September 27, 2023 4:35 AM
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Book chapter on biocatalysis of steroids

Book chapter on biocatalysis of steroids | iBB | Scoop.it

Mycobacterium sp. can convert steroids such as β-sitosterol, campesterol, and cholesterol, into key intermediates that can be easily functionalized to yield commercially interesting pharmaceutical products. In a chapter published in the 2nd edition of the book “Microbial Steroids – Methods and Protocols”, Carla C.C.R. de Carvalho and Pedro Fernandes, describe the methodology for the biocatalysis of β-sitosterol to steroids, both in aqueous and organic:aqueous systems using traditional organic and green solvents.

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July 20, 2023 1:00 PM
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Annual meeting of project FuturEnzyme

Annual meeting of project FuturEnzyme | iBB | Scoop.it

Carla C. C. R. de Carvalho and Ricardo Pereira (2BRG-iBB) participated in the annual meeting of the EU funded project FuturEnzyme (https://www.futurenzyme.eu). The 16 partners that integrate the project met during July 6 and 7, in the house of UHAM members, the Institute of Plant Science and Microbiology (IPM, Hamburg, Germany), next to the Botanical Garden. The work carried out during the first 24M of the project was presented and discussed between partners and in the presence of members of the Advisory Board.

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https://www.futurenzyme.eu/meetings/general-assembly-meeting-hamburg-germany/

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June 12, 2023 2:11 PM
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1st BSC Workshop on Computational Enzyme Bioprospecting and Engineering

1st BSC Workshop on Computational Enzyme Bioprospecting and Engineering | iBB | Scoop.it

In the past 22nd and 23rd of May, PhD students from the Biotechnology and Biosciences doctoral program, Luís Sousa and Ricardo Pereira, attended the First Workshop on Computational Enzyme Bioprospecting and Engineering at the Barcelona Supercomputing Center (BSC). As a part of a joint program between two European projects, Oxipro (DOI 10.3030/101000607) and FuturEnzyme (DOI 10.3030/101000327), the last where IST-ID is a working partner headed by  Carla C. C. R. de Carvalho (iBB), the attendees were able to use the leading softwares for computational protein engineering. With Galaxy, attendees were able to create and develop their own workflows for Computational Bioprospecting, HMM routines, and basic sequence similarity analysis. For Protein Engineering, the Schrodinger Suite and the AsiteDesign Galaxy were made available to perform analysis and visualization of enzyme structures, perform tasks such as system preparation, visualization, sitemap calculations, ligand docking and enzyme redesign. The workshop promoted interdisciplinary thinking and collaboration between different fields, highlighted the real-world applications of computational protein engineering, and its importance in advancing our understanding of biological systems and developing new solutions for global challenges.

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March 27, 2023 11:54 AM
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Biocatalysis, Enzyme and Process Engineering

Biocatalysis, Enzyme and Process Engineering | iBB | Scoop.it

Enzymes may be the right catalysts for efficient and clean processes, contributing to energy savings and negligible by-products and waste. Following the success of the Special Issue on “Biocatalysis, Enzyme and Process Engineering” in Processes (MDPI), the editors Pedro Fernandes and Carla C. C. R. de Carvalho wrote an editorial paper discussing the different papers published on this subject.

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Check special issue at: https://doi.org/10.3390/pr11030740

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December 1, 2022 8:19 AM
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Bacterial contamination of coins

Bacterial contamination of coins | iBB | Scoop.it

Microbes are known to contaminate money. In the project CleanCoin, funded by INCM, Carla C.C.R. de Carvalho (iBB), Telmo Santos (UNIDEMI, UNL) and Maria José Caramujo (cE3c, FCUL, UL), studied the bacterial population present at the surface of coins. In a recently published white paper, the research team and collaborators from INCM, present the results and compare the number of viable bacteria in coins with those found on other objects such as bank cards and mobile phones.

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September 6, 2022 11:10 AM
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Adaptation of Bacteria to Antineoplastic Agents

Adaptation of Bacteria to Antineoplastic Agents | iBB | Scoop.it

The increasing number of life-threatening infections observed in cancer patients has been ascribed to chemotherapy-induced neutropenia and to invasive medical procedures. In a recent published study, Carla C.C.R. de Carvalho (BERG-iBB) questioned if the infections could also be favored by an increased resistance of bacteria due to the adaptation to antineoplastic agents used in chemotherapy. After exposure to several antineoplastic agents, it was observed that cells of Staphylococcus aureus, Mycobacterium vaccae, Pseudomonas aeruginosa, and Escherichia coli changed the fatty acid profile of their cellular membranes, produced exopolymeric substances, and formed aggregates that adhered to surfaces. Additionally, when exposed to high concentrations of these compounds, a persister sub-population could be identified. After adaptation to antineoplastic agents, the minimum inhibitory concentration (MIC) of several antibiotics increased considerably in the tested strains. This should be a major concern during oncology treatments.

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https://www.mdpi.com/2306-5354/9/8/355

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July 13, 2022 3:54 AM
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Edible Flowers of Helichrysum italicum

Edible Flowers of Helichrysum italicum | iBB | Scoop.it

Helichrysum italicum is a halophyte shrub with bright yellow flowers with a strong curry-like aroma that have been used in the production of cosmetics and pharmaceuticals, for seasoning and flavoring of food, and as natural food preservatives. In a study  recently published in Food Research International, researchers of Universidade Nova de Lisboa, Polytechnic of Leiria, University of Coimbra, University of Lincoln, the Quadram Institute, and Carla C.C.R. de Carvalho (BERG-iBB), characterize the composition, bioactive compounds, and nutritive value of H. italicum flowers. The flowers were found to be a good source of minerals, omega-6 and omega-3 PUFAs, polyphenols, carotenoids, curcuminoids and other bioactive compounds with relevant antibacterial, antifungal and antioxidant properties.

 

Photo details: Helichrysum italicum, Bj.schoenmakers, CC0, via Wikimedia Commons.

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July 5, 2022 11:48 AM
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Participation in “One Sustainable Ocean”

Participation in “One Sustainable Ocean” | iBB | Scoop.it

Carla CCR de Carvalho (iBB-BERG) presented the work entitled “Marine bioprocesses with bacteria from the Portuguese coast” at the “One Sustainable Ocean”, a side event of the UN Ocean conference (UNOC) held in Lisbon. The UN Ocean Conference, co-hosted by the Governments of Kenya and Portugal, aimed at starting a new chapter of global ocean action.

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May 16, 2022 4:56 AM
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Benzyl Alcohol Production in Stirred and Packed Bed Reactors

Benzyl Alcohol Production in Stirred and Packed Bed Reactors | iBB | Scoop.it

The ocean is an excellent source for new biocatalysts due to the tremendous genetic diversity of marine microorganisms, and it may contribute to the development of sustainable industrial processes. In a recent paper published in Microorganisms, Carlos J.C. Rodrigues and Carla C.C.R. de Carvalho (iBB-BERG) used a marine bacterium for the conversion of benzaldehyde to benzyl alcohol, which is an important chemical employed as a precursor for producing esters for cosmetics and other industries. A stirred reactor, using a fed-batch approach, enabled a 1.5-fold increase in benzyl alcohol productivity when compared with batch mode. However, product accumulation in the reactor hindered the conversion. The use of a continuous flow reactor packed with immobilized cells enabled a 9.5-fold increase in productivity when compared with the fed-batch stirred reactor system.

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January 28, 2022 4:13 AM
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ChiCoBionts: Chitinases from the Octocoral Microbiome

ChiCoBionts: Chitinases from the Octocoral Microbiome | iBB | Scoop.it

ChiCoBionts, a new FCT-funded research project, exploits the octocoral microbiome in the search for novel chitinases of relevance to global biogeochemical cycling, food waste management, and the smart production of added-value molecules. The project starts in January 2022, is funded with 50,000 euros, and aims to discover, express, and characterise novel chitinases and chitinolytic microbes with the potential to outperform current commercial enzymes and to foster the development of blue biotechnology for chitin waste. The ChiCoBionts team joins iBB scientists from BSRG and BERG, including Tina Keller-Costa (PI), Ângela Taipa (Co-PI), Carla de Carvalho and Rodrigo Costa, in addition to ITQB researcher Nuno Borges and collaborators from the University of Brussels, Belgium.

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January 5, 2022 12:22 PM
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Project YumAlgae

Project YumAlgae | iBB | Scoop.it

Consumer acceptance of microalgae products is often hampered by their colour, “fishy” smell and flavor. An international project with research teams from ISA (PI Prof. Anabela Raymundo), IST (Carla de Carvalho, iBB-BERG), Norce (Norway) and the company Pagarete Microalgae solutions (Portugal) is aimed at the use of enzymes to improve the overall sensory quality of microalgal ingredients. The project, entitled “YUM ALGAE - enzYmes for improved sensory qUality of MicroALGAE ingredients in foods”, is funded in ca. 1 million euros by the Iceland, Liechtenstein, Norway grants (EEA grants, Blue Growth programme).

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March 22, 11:04 AM
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Participation in the World Ocean Summit & Expo

Participation in the World Ocean Summit & Expo | iBB | Scoop.it

Carla CCR de Carvalho participated in the 11th annual World Ocean Summit & Expo, following an invitation from the organizers. The summit, organized by the Economist Impact, joined over 1500 attendees, from more than 80 countries, to discuss ocean health, industry strategies for a sustainable ocean economy, and ocean-climate solutions. The agenda included sessions such as Blue Food, Ocean health, and Shipping and ports, as well as innovation showcases, “How to” sessions, and networking.

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November 30, 2023 2:40 PM
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Characterization of a strain from a shallow water hydrothermal vent

Characterization of a strain from a shallow water hydrothermal vent | iBB | Scoop.it

Microbial life present in the marine environment has to be able to adapt to rapidly changing and often extreme conditions. In a recently published paper, Ricardo F.S. Pereira and Carla C.C.R. de Carvalho form iBB and colleagues from CQE and ISEL, used genomic, lipidomic and biochemical approaches to characterise a Serratia rubidaea, isolated from a sample taken at a shallow water hydrothermal vent, and its red product. Several state-of-the-art techniques, such as DSC, FTIR, NMR, and Ultra-High Resolution Qq-Time-of-Flight mass spectrometry, were used to characterize the structure of the red pigment which was found to be prodigiosin.

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September 18, 2023 5:24 AM
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Meeting of project Yum Algae in Bergen

Meeting of project Yum Algae in Bergen | iBB | Scoop.it

Carla C. C. R. de Carvalho, Kilian Neves and Sebastião Tavares participated in the annual meeting of project Yum Algae, funded by programme Blue Growth of EEA Grants. The Yum Algae researchers from Instituto Superior Técnico, Instituto Superior de Agronomia, NORCE Norwegian Research Centre, and Pagarete Solutions, met at NORCE facilities in Bergen, Norway. The results presented are very promising to improve the sensory quality of microalgae ingredients in foods such as bread and cheese. Future actions were planned for the scale up of the process.

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July 20, 2023 12:59 PM
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Participation in FEMS2023

Participation in FEMS2023 | iBB | Scoop.it

Carla CCR de Carvalho and the Ph.D. student Ricardo FS Pereira presented their work during the FEMS2023 congress in Hamburg, Germany. The congress organized by Federation of European Microbiological Societies is the largest event in the field of microbiology in Europe. Carla de Carvalho presented the talk entitled “Development of efficient bioprocesses using isolated marine bacteria” in the Industrial Microbiology and Bioeconomy session. Ricardo Pereira did a flash presentation entitled “Marine bioprocess development: targeting the production of prodigiosin” during the Biotechnology & Synthetic Microbiology session.

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https://www.fems2023.org/

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May 26, 2023 6:48 AM
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Extract of a new haloarchaea to fight inflammatory diseases

Extract of a new haloarchaea to fight inflammatory diseases | iBB | Scoop.it

Pathologies such as psoriasis and rheumatoid arthritis have an inflammatory base triggered, in part, by an impairment of antioxidant defense systems which leads to overaccumulation of oxidative species (ROS). In a recently published paper, entitled “Up-Regulation of the Nrf2/HO-1 Antioxidant Pathway in Macrophages by an Extract from a New Halophilic Archaea Isolated in Odiel Saltworks”, researchers of the Universidad de Sevilla, Universidad de Huelva and Carla C. C. R. de Carvalho (iBB), show that an extract of Haloarcula sp. OS, containing bacterioruberin and significant amounts of fatty acids, exhibits high antioxidant activity. The study shows, for the first time, that the extract reduces LPS-induced oxidative stress and acute inflammation in THP-1 macrophages by attenuating both ROS levels and the production of the pro-inflammatory cytokines TNF-a and IL-6. The mechanism involves the activation of the Nrf-2/HO-1signaling pathway, and supports the use of the extract as a therapeutic agent in the treatment of oxidative stress-related inflammatory diseases.

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December 1, 2022 10:11 AM
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Marine Bioprospecting, Biocatalysis and Process Development

Marine Bioprospecting, Biocatalysis and Process Development | iBB | Scoop.it

Marine bacteria present enzymatic activities that result from extensive evolution necessary for cell survival under the harsh and continuously changing conditions found in the marine environment. Metagenomic techniques and cultivation of not-yet-cultured bacteria, combined with high-throughput screening techniques, have allowed the identification of new biocatalysts and the development of industrial processes. In a recently published paper, Carlos J.C. Rodrigues and Carla C.C.R. de Carvalho (iBB) review the latest studies related to growth of marine bacteria under laboratorial conditions, screening techniques, and bioprocess development.

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October 26, 2022 10:46 AM
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World’s Top Scientists

World’s Top Scientists | iBB | Scoop.it

Six researchers from @IBB have been ranked among the World’s Top 2% of most widely cited scientists in the 2022 List of outstanding researchers prepared by Elsevier BV, and Stanford University (USA).   

Carla Carvalho, Joaquim Cabral, Isabel Sá Correia,  Mário Berberan e Santos, Miguel Prazeres and Pedro Fonte were recognized for the impact throughout their careers, while Pedro Fernandes was recognized as one of the World’s Top 2%  influent scientist in his field.

The updated list is based on the Sep 01-2022 SCOPUS database, updated to the end of the citation year 2021 and analyses 22 scientific areas and 176 disciplines.

Overall, 93 researchers from Técnico were highlighted in the study.  Congratulations to all!

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Congratulations!

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September 6, 2022 11:04 AM
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Overcoming the “unculturable” dogma

Overcoming the “unculturable” dogma | iBB | Scoop.it

It is usually accepted that only 0.1-1% of marine bacteria may be cultivated under laboratory conditions. This resulted mainly from the observation that only a small fraction of the total bacteria determined by microscopic counts could be enumerated by culture plate techniques, and has been a justification for the widely implementation of cultivation-independent molecular techniques. In a recent published paper, Carlos J.C. Rodrigues and Carla C.C.R. de Carvalho (BERG-iBB) promoted the growth of bacteria from a marine rock pond by culture dependent techniques and compared the results with culture independent methods. The highest efficiency of cultivation was 45% attained in marine agar. The study shows that it is possible to grow more than the traditionally considered 1% of bacteria from a seawater sample using standard agar plate techniques and laboratorial conditions, and to find bacteria not detected by molecular approaches.

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Check paper at:

https://www.frontiersin.org/articles/10.3389/fbioe.2022.964589/full

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July 5, 2022 11:51 AM
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Visit of Ministers of Norway – Project Yum Algae

Visit of Ministers of Norway – Project Yum Algae | iBB | Scoop.it

The Norwegian Minister of Fisheries and Ocean Policy Bjørnar Selnes Skjæran, the Minister of International Development Anne Beathe Tvinnereim, and the Embassador in Lisbon Tove Bruvik Westberg, visited the Instituto Superior de Agronomia as promotor of projects funded by EEA grants. Anabela Raymundo and Cristiana Nunes (LEAF-ISA), Carla de Carvalho (iBB-IST) and António Pagarete (Pagarete Microalgae Solutions) presented the collaborations they have had with Norwegian research groups including the project Yum Algae. This projects aims at finding enzymes to improve the sensory quality of microalgae ingredients in foods and during the visit several products, such as bread, cheese and ice cream containing microalgae, were tasted. The visit also included members of the “Unidade Nacional de Gestão” of EEA Grants and of “Direção Geral de Política do Mar”.

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July 4, 2022 10:06 AM
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Carlos Rodrigues to Defend PhD Thesis in Biotechnology and Biosciences

Carlos Rodrigues to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Carlos Júnio Caridade Rodrigues will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, Friday the 26th of July 2022, at 10:00 am (Amphitheater PA-3, floor -1, Mathematics building, Alameda campus). During the last years, and under the supervision of Carla Carvalho from iBB, Carlos focused on the development of novel integrative bioprocesses by accessing the enzymatic machinery of marine bacteria and improving cultivation techniques of ‘unculturable’ bacteria. The title of his thesis is “Developing bioprocesses with marine bacteria for sustainable industrial applications". Check some of Carlo´s PhD outputs here.

Ana Azevedo's comment, July 22, 2022 3:15 AM
Best of luck Carlos!
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March 31, 2022 5:38 AM
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Detection of mcr-1 gene in Vibrio Species Isolated From Clams

Detection of mcr-1 gene in Vibrio Species Isolated From Clams | iBB | Scoop.it

Pathogenic Vibrio species are responsible for human and animal illness and one of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filter-feeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In a recently published study carried out by researchers of ESTM, Polytechnic of Leiria, and Carla C.C.R. de Carvalho (BERG-iBB), the profile of antimicrobial resistance and the presence of virulence genes in the bacterial isolates from clams were evaluated. The presence of the mobilisable colistin resistance gene mcr-1 in three Vibrio spp. isolates highlights a potential threat to public health.

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January 11, 2022 12:39 PM
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Integral Analysis of Liquid-Hot-Water Pretreatment of Wheat Straw

Integral Analysis of Liquid-Hot-Water Pretreatment of Wheat Straw | iBB | Scoop.it

Developing viable biorefineries is urgent to support the transition to a sustainable society. Lignocellulosic biomass is a crucial renewable feedstock, but usually a combination of pretreatments is necessary to extract both sugars and lignin. In a recent paper published in the journal Sustainability, the M.Sc. student Manuel Dias and Carla C.C.R. de Carvalho (BERG-iBB) collaborated with researchers from Technische Universität Wien (Vienna, Austria) under supervision of Prof Anton Friedl, to study liquid-hot-water (LHW) as pretreatment for hemicellulose hydrolysis. Assessment of different LHW conditions allowed the determination of those leading to high sugar concentration and low concentration of degradation products. The lignin remaining in the solid phase could be extracted and hydrolysed. By including the analysis of lignin hydrolysis into the standard sugar and degradation products in LHW, it was possible to make an integrated decision to valorize the different lignocellulosic components of wheat straw.

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