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Ana Margarida Rosa to Defend PhD Thesis in Biotechnology and Biosciences

Ana Margarida Rosa to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Ana Margarida Rosa will be defending her PhD thesis in Biotechnology and Biosciences on Wednesday the 29th january 2020 (10:30 H, amphitheater PA-3, Mathematics Building). During the last years, and under the supervision of Miguel Prazeres from BERG-iBB and Pedro Paulo from CQE-IST, Ana developed a cellulose-based system for the colorimetric detection of nucleic acids by exploring the recognition of biotin-labeled DNA hybrids by anti-biotin antibodies immobilized through ZZ-CBM chimeric proteins. These fusions combine carbohydrate-binding modules (CBM) with affinity to cellulose and double Z domains (ZZ) that bind to IgG antibodies. The title of the thesis is “DNA recognition system on bioactive paper using fusions of carbohydrate-binding modules and IgG-binding Z domains”.

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Fluorescence Correlation Spectroscopy of Protein:DNA Supramolecular Complexes

Fluorescence Correlation Spectroscopy of Protein:DNA Supramolecular Complexes | iBB | Scoop.it

Protein:DNA supramolecular complexes are at the heart of many molecular diagnostic systems. In a paper just published in Physical Chemistry Chemical Physics, Ana Rosa and Miguel Prazeres from BERG-iBB and Pedro Paulo from CQE present a fluorescence correlation spectroscopy (FCS) study of the complexation of oligonucleotides, antibodies and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module. The FCS-derived information obtained (diffusion coefficients, association constants, nanometer-scale size of complexes) is useful to gain insight into molecular interactions involved in diagnostic assays. Click on title to learn more.

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Featured Photo: Microscopic Structure of Chromatographic Paper (100X)

Featured Photo: Microscopic Structure of Chromatographic Paper (100X) | iBB | Scoop.it

Description: Microscopic structure of chromatographic paper (100 Xs), featured photo by Ana Rosa and Miguel Prazeres, Copyright BERG-iBB 2016.


Context: BERG researchers led by Miguel Prazeres are developing paper-based biosensors for molecular diagnostics by combining affinity-based anchoring of biomolecules with nanoparticle-based detection. The work is being performed in the context of iBB’s Strategic Area 2: Advanced Bioprocess Engineering.

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Detection of DNA on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles

Detection of DNA on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles | iBB | Scoop.it

Nucleic acid testing requires skilled personnel and expensive instrumentation and are thus difficult to implement in low income settings. In a paper published in the Biotechnology Journal, researchers from BERG-iBB describe a method for the detection of oligonucleotides that combines cellulose microparticles with biomolecular recognition. Cellulose microparticles are made bioactive by anchoring anti-biotin antibodies via ZZ-CBM fusions that combine a carbohydrate binding module with the ZZ fragment of protein A. Samples are prepared by incubating DNA probes immobilized on gold nanoparticles (AuNP) with biotin-labeled targets and then mixed with bioactive microparticles. The presence of un-labelled targets could also be probed by introducing a second, biotinylated DNA probe. The target:probe-AuNP hybrids are mixed with and captured by the microparticles, which change color from white to red. With this methodology, diverse analytical applications can be envisaged based on “reader-free” testing. The work was performed in the context of the project CBM-X.

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Biomolecular Functionalization of Cellulose with Gold Nanoparticles

Biomolecular Functionalization of Cellulose with Gold Nanoparticles | iBB | Scoop.it

Materials with new and improved functionalities can be obtained by modifying cellulose with gold nanoparticles (AuNPs). In a paper published in the Journal of Molecular Recognition, António Almeida, Ana Rosa, Ana Azevedo and Miguel Prazeres from BERG-iBB, describe a method to functionalize cellulose with AuNPs that relies on 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module with the ZZ fragment of protein A and an anti-biotin antibody. Paper and cellulose microparticles with AuNPs immobilized via the ZZ-CBM3:anti-biotin IgG supramolecular complex displayed an intense red color, whereas no color is detected when AuNPs are deposited over the unmodified materials. The method also opens up the way for the development of simple and straightforward paper/cellulose-based tests where detection of a target analyte can be made by direct use of color signaling. Click on title to learn more.

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