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January 17, 4:48 AM
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Ana Melo received a Visiting Researcher Award from FAPESP

Ana Melo received a Visiting Researcher Award from FAPESP | iBB | Scoop.it

Ana Melo obtained a Visiting Researcher Award from the São Paulo Research Foundation (FAPESP), Brazil. This funding allowed her to stay in Brazil during October 2023 for: i) Visiting the Laboratory of Professor Rosangela Itri at Physics Institute, University of São Paulo (IFUSP); ii) Performing measurements at the Brazilian Synchrotron Sirius -4th Generation (Campinas, São Paulo State); Iii) Participating in the 47th Annual Meeting of Brazilian Biophysical Society (SBBf, Unicamp Convention Center from 19 to 22 October 2023).

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July 20, 2023 12:38 PM
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Ana Melo gave an invited talk at ELIXIR IDP Community Meeting

Ana Melo gave an invited talk at ELIXIR IDP Community Meeting | iBB | Scoop.it

Ana Melo was invited to participate in the ELIXIR IDP Community F2F/hybrid meeting that took place on July 3-4, in Bratislava- Slovakia. The meeting aimed to “develop standards, tools and resources to accelerate the identification, analysis and functional characterization of intrinsically disordered proteins (IDPs).”

Ana Melo gave an invited talk focused on probing the conformational dynamics of disordered proteins involved in neurodegeneration- as tau protein and huntingtin exon 1. She had the opportunity to discuss her main research strategies for studying aggregation-prone disordered proteins (IDPs) using advanced fluorescence methods.

 

 

 

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July 26, 2022 11:59 AM
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Ana Melo awarded the FEBS Letters Poster Prize at the "Biochemistry Global Summit 2022"

Ana Melo awarded the FEBS Letters Poster Prize at the "Biochemistry Global Summit 2022" | iBB | Scoop.it

Ana Melo received the FEBS Letters Poster Prize (Day #3) at the "Biochemistry Global Summit 2022", a joint meeting from the Federation of European Biochemical Societies (FEBS), the International Union of Biochemistry and Molecular Biology (IUBMB) and the Pan-American Association for Biochemistry and Molecular Biology (PABMB), this July 2022 at Lisbon. AM Melo presented her work focused on the characterization of the membrane-bound state of huntingtin exon 1 and its implications in Huntington´s disease. 

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April 22, 2022 9:57 AM
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Ana Melo Presents and Co-chairs at the 66th Biophysical Society Annual Meeting

Ana Melo Presents and Co-chairs at the 66th Biophysical Society Annual Meeting | iBB | Scoop.it

Ana Melo from BSIRG-iBB delivered an oral presentation at the 66th Biophysical Society Annual (BPS) Meeting, San Francisco, California (February 2022). The focus was on the characterization of the distinct conformational signatures of the flanking polyQ regions in the membrane-bound state of Huntington exon 1, and its implications in Huntington´s disease. Ana was also invited by the Scientific Committee to co-chair the Intrinsically Disordered Proteins (IDPs) section together with Martin Fossat from Washington University (St Louis). The BPS meeting is the largest and most reputed worldwide Biophysical Meeting, and this year was attended by biophysicists from 46 countries. In Ana´s section, researchers from Univ. Cambridge, Yale Univ., Washington Univ. St Louis, Univ. of Copenhagen and Rensselaer Polytechnic Institute presented research focused on distinct biophysical features of IDPs associated with function and dysfunction.

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September 3, 2021 10:50 AM
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Lipid Hydroperoxide Compromises the Membrane Structure Organization

Lipid Hydroperoxide Compromises the Membrane Structure Organization | iBB | Scoop.it

Lipid hydroperoxides have recently been recognized as key mediators of diseases (such as neurodegenerative disorders or Type II diabetes) and cell death. In a recent work, structural and dynamic perturbations induced by the hydroperoxidized POPC lipid (POPC-OOH) in fluid POPC membranes were addressed using advanced small-angle X-ray scattering (SAXS) and fluorescence methodologies. Notably, this multidisciplinary approach revealed that the hydroperoxide group located at the membrane interface, promotes a higher membrane hydration and microviscosity, with a strikingly lower order and bending rigidity, an unusual trend in membrane biophysics, which ultimately compromises membrane structure organization. This international work co-led by Ana M. Melo (BSIRG-iBB) and Rosangela Itri (Institute of Physics, University of São Paulo) was recently published in Langmuir and involved other BSIRG-iBB researchers (Ana Coutinho, Alexander Fedorov and Manuel Prieto).

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January 21, 2020 9:53 AM
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The Conformational Polymorphism of Disordered Proteins in Neurodegeneration at the Single-molecule Level

The Conformational Polymorphism of Disordered Proteins in Neurodegeneration at the Single-molecule Level | iBB | Scoop.it

Intrinsically disordered proteins/regions (IDPs/IDRs) are widespread in the human proteome and play critical roles in distinct biological processes and dysfunction. Contrary to the classical “structure–function” paradigm, IDPs lack stable secondary/tertiary structures under physiological conditions, and instead adopt a dynamic ensemble of multiple conformations. Moreover, several neurodegenerative disorders are associated with the pathological self-assembly of neuronal IDPs, including tau (Alzheimer’s disease), α-synuclein (Parkinson’s disease), and huntingtin exon 1 (Huntington’s disease). Therefore, there is an emerging medical interest in understanding their physical and structural features. However, their characterization is inherently challenging by traditional ensemble and time-averaging methodologies. A recent review co-authored by Ana Melo from BSIRG-iBB, and published in Frontiers in Molecular Neurosciences the advantages of employing cutting-edge single-molecule fluorescence techniques are discussed to characterize the conformational ensemble of neurodegeneration-promoting IDPs under normal and disease conditions to obtain insights into their gain- or loss-of-function.

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January 31, 2019 6:49 AM
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Research Scientist Position in Biophysics

Research Scientist Position in Biophysics | iBB | Scoop.it

A position is open to hire a doctorate researcher within the framework of the FCT-funded SingleMolHTTex1 project. The overall aim of this project is to provide a detailed understanding of the initial binding steps of Huntingtin exon1 (HTTex1, with an expanded polyglutamine repeat) to biological membranes. A complementary set of single-molecule fluorescence techniques will be used to obtain insights into the physical/structural features of aggregation-prone states of HTTex1 at the membrane surface. Importantly, the results of this frontier project may assist in the rational design of inhibitors to treat and/or prevent Huntington’s disease. The work will be carried out under the scientific guidance of Dr. Ana M. Melo and at BSIRG (at CQFM and iBB), IST. More information on the position and how to apply can be found here under the acronym SingleMolHTTex1 and email to anammelo@tecnico.ulisboa.pt. The application deadline is February 12th, 2019.

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July 20, 2023 12:39 PM
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Ana Melo presented and was elected for co-leader of Working Group 4 of the ML4NGP COST-Action

Ana Melo presented and was elected for co-leader of Working Group 4 of the ML4NGP COST-Action | iBB | Scoop.it

Ana Melo delivered an oral presentation at the 1st ML4NGP Meeting on Machine Learning and Non-Globular Proteins, Bratislava- Slovakia (July 2023). Her presentation was focused on mapping the structural transitions of the flanking polyQ regions in huntingtin exon 1 upon membrane binding, and its implications in Huntington´s disease. 

AM Melo was also elected for co-Leader of Working Group 4 of this ML4NGP COST-action. This group aims to improve the functional characterization of non-globular proteins (NGPs) and to understand the major questions involving NGPs. They include “conformational heterogeneity, evolution, conserved motifs, protein-protein interactions, liquid–liquid phase separation (LLPS) and involvement in neurodegenerative diseases.”

 

 

iBB's insight:

More details of the Core Group in https://ml4ngp.eu/team-2/

and Research in https://ml4ngp.eu/research/

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November 25, 2022 9:18 AM
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Ana M Melo was nominated for the Management Committee of the COST Action ML4NGP

Ana M Melo was nominated for the Management Committee of the COST Action ML4NGP | iBB | Scoop.it

Ana M Melo participated as secondary proposer in the grant application of the COST Action: Non-globular proteins in the era of Machine Learning (ML4NGP). The action was recently funded and started in October 2022. Ana M Melo was selected to be part of the Management Committee and the first meeting already took place with decisions on the budget allocation/organization and future meetings. This COST-action is focused on non-globular proteins that comprise “intrinsically disordered regions, repeats, low-complexity sequences, aggregation-prone and phase-separating sequences, and are implicated in a range of age-related diseases”. The network combines both experimentalists and computational researchers. 

iBB's insight:

More details in https://www.cost.eu/actions/CA21160/#tabs+Name:Description.

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April 22, 2022 12:27 PM
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Ana Melo Receives a 2022 Biophysical Travel Award

Ana Melo Receives a 2022 Biophysical Travel Award | iBB | Scoop.it

Ana M. Melo was a recipient of a 2022 Biophysical Travel Award for Scientists within 10-years after completing the PhD. She was honored during the Travel Awardee Reception by the Society’s Committees for Inclusion/Diversity and Professional Opportunities for Women. This highly competitive award covered her travel expenses to San Francisco, California (US).

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January 5, 2022 12:05 PM
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Ana Melo Delivers Invited Seminar at the Portuguese Neuroscience Meeting

Ana Melo Delivers Invited Seminar at the Portuguese Neuroscience Meeting | iBB | Scoop.it

Ana Melo gave an invited talk during the XVII Meeting of the Portuguese Society for Neuroscience, at Coimbra, 1 - 3 December, 2021. She presented recent work focused on characterizing the distinct conformational dynamics of the flanking polyQ regions in the membrane-bound state of Huntington exon 1, and its role in Huntington´s disease. Her presentation was in the framework of the SPB Symposium: “A biophysical perspective on degenerative diseases: protein assembly and phase separation”, together with Sandra Macedo Ribeiro (IBMC- i3S) and Salvador Ventura (IBB-Universitat Autonoma de Barcelona).

 

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March 10, 2021 11:04 AM
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Portuguese Platform of BioImaging is Now a Euro-Bioimaging Node

Portuguese Platform of BioImaging is Now a Euro-Bioimaging Node | iBB | Scoop.it

The Portuguese Platform of BioImaging (PPBI) is now a Euro-Bioimaging Node. The Advanced Fluorescence Microscopy Facility at iBB-IST joined this application, and now also integrates the Euro-BioImaging network. Ana M. Melo (Facility Head) presented on 22nd February 2021 our site, specialized in quantitative imaging and dedicated to functional imaging and single-molecule techniques.

 

 

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June 21, 2019 5:11 AM
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Twin Seminars on Dysfunctional Disordered Proteins and Anticancer Activity of Azurins

Twin Seminars on Dysfunctional Disordered Proteins and Anticancer Activity of Azurins | iBB | Scoop.it

The 3rd Edition of iBB seminars will continue on the 24th June with short talks from Ana Melo - "Untangling the role of dysfunctional disordered proteins in neurodegeneration at single molecule level" and Nuno Bernardes - "Exploring plasma membrane components' organization and dynamics as targets for azurins: their role in anticancer activity and selective uptake of therapeutics". Join us next monday (13h00 h, room 1.38, IST-Tagus Park) to learn more about Ana's and Nuno's research.

 

Photo details: 3D-model of oxidized Pseudomonas aeruginosa azurin (pdb 4AZU)

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