Purification of mAbs in a UF Stirred Cell | iBB | Scoop.it

Charged ultrafiltration membranes combined with optimal operating conditions can be used to enable the purification of monoclonal antibodies. Researchers at BERG-iBB (Alexandra Wagner, Marilia Mateus and Ana Azevedo) and BSIRG-IBB (Ana Ferraria and Ana Maria Botelho do Rego) have studied the influence of polyethersulfone membrane modification with polyethyleneimine and operating conditions on the removal of impurities from a mAb-containing cell culture fluid. High purity was consistently observed at pH 9 due to a simultaneous decrease in the transmission of soluble protein and increase in both IgG transmission and adsorptive capacity of the membranes. Click on tittle to learn more in the Journal of Chemical Technology and Biotechnology.