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An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms 
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The authors demonstrate that incorporation of appropriately modified bacteria into the gut microbiota has potential as an effective strategy to inhibit the development of metabolic disorders. Indeed, mice that received modified bacteria had dramatically lower food intake, adiposity, insulin resistance, and hepatosteatosis compared with mice receiving standard water or control bacteria.
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The authors have uncovered an unexpected, neomorphic insulin-sensitizing action for exogenous non-mitogenic human FGF1 with therapeutic potential for the treatment of insulin resistance and type 2 diabetes.
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The GLP posts a collection of the responses from scientists worldwide to the republication of a controversial animal study on GM corn and herbicide that had
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A couple of years ago, my fiancée and I wanted to try to make some home-made mozzarella cheese, but ran into a problem. In order to ...
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From
phys
Genetic engineering of plants, animals and microorganisms such as bacteria typically involves the use of restriction enzymes to 'cut and paste' DNA fragments into certain genetic sequence locations. This process allows scientists to introduce new genes into an organism, but is constrained to specific ...
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Artist prints 3D replica of van Gogh's ear grown from living cells of his great-great grandson.
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The authors' observations suggest a mechanism for a Protospacer Adjacent Motif (PAM)-dependent target DNA melting and RNA–DNA hybrid formation. Furthermore, this study establishes a framework for the rational engineering of Cas9 enzymes with novel PAM specificities.
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The authors enhanced the integration site-specificity of the phiC31 integrase-based vector using a sequence-specific DNA-binding protein containing Gal4 and LexA DNA-binding motifs. In human cells, they showed thanks to quantitative PCR that integration around the LexA operator was 26-fold higher than with the control.
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The authors design a new technique to examine how proteins interact with each other at the level of a single HIV viral particle.
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The CRISPR system has attracted considerable attention for its potential uses in genetic engineering and biotechnology, but its roles in bacterial gene regulation are still surprising scientists. The authors find that when the gene encoding Cas9 is mutated in F. novicida bacteria, they become more vulnerable to polymyxin B as well as standard antibiotic treatments such as streptomycin and kanamycin. They were able to trace the effects of the mutation back to a defect in "envelope integrity." Cas9 regulates production of a lipoprotein, which appears to alter membrane permeability.
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BigField GEG Tech's insight:
The authors used CRISPR-Cas9 and TALEN to edit the genome of human pluripotent stem cell clones and they performed whole-genome sequencing at high coverage in order to assess the degree of mutagenesis across the entire genome. In both types of clones, they found that off-target mutations attributable to the nucleases were very rare. From this analysis, they suggest that, although some cell types may be at risk for off-target mutations, the incidence of such effects in human pluripotent stem cells may be sufficiently low and thus not a significant concern for disease modeling and other applications.
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
BigField GEG Tech's insight:
Smart strategy which allows a very simple, efficient and fast assay for detecting induced mutations It will be surely widely used in the area of genome editing.
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If you're going to Brazil for the World Cup, don’t forget your insect repellent, and maybe one of these. The country has the highest incidence of dengue fever in the world, with...
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Oxitec has developed transgenic mosquitoes. The modified male mosquitoes are released into the wild and create progeny that have the lethal gene.
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The rise of antibiotic resistance rekindles interest in a century-old virus treatment.
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The tangled highway of blood vessels that twists and turns inside our bodies, delivering essential nutrients and disposing of hazardous waste to keep our organs working properly has been a conundrum for scientists trying to make artificial vessels from scratch.
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The authors combined CRISPR/Cas9 technology with the piggyBac transposon to efficiently correct the HBB mutations in patient-derived iPSCs without leaving any residual footprint. No off-target effects were detected in the corrected iPSCs, and the cells retain full pluripotency and exhibit normal karyotypes. When differentiated into erythroblasts gene-corrected iPSCs restored expression of HBB compared to the parental iPSCs line.
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