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E unus pluribum (out of one person, many genomes): Genome Biology at the CSH Single Cell Analyses meeting

E unus pluribum (out of one person, many genomes): Genome Biology at the CSH Single Cell Analyses meeting | from Flow Cytometry to Cytomics | Scoop.it
You only get one shot, do not miss your chance to blow Eminem, 'Lose Yourself' Hip hop has-been Marshall Mathers III may or may not have been musing on the technical challenges of single-cell sequencing when he uttered these words, but he might as...
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Counting Cells with a Hemocytometer

There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted?

 

Gilbert C FAUREs insight:

It is how techniques are taught or learned now! Thanks Youtube

Obviously, this first Scoop is not significant of the information you will find through the following 122 pages of this topic, with many recent informations of developments of flow cytometry, image cytometry... focusing particularly on the detection, quantification, characterization of rare events (CTCs, CECS..) in peripheral blood and biological fluids.

 

more than 3000 scoops, >5200 visitors, 10700 views (march 2015)

juin 2019 more than 4400 scoops, >13K visitors, >31,6 K views

 

For data from the curator, see the Nancytomique topic.

http://www.scoop.it/t/nancytomique

 

 

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Liquid Biopsy ONLINE - Webinar 3: Liquid Biopsy in a clinical setting

Liquid Biopsy ONLINE - Webinar 3: Liquid Biopsy in a clinical setting | from Flow Cytometry to Cytomics | Scoop.it
As we continue to better our understanding of cancer biology, one thing remains a game changer for cancer care: the ability to detect cancer in a liquid biopsy.Just a few years ago, the idea of hav...
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Tumor circulome in the liquid biopsies for cancer diagnosis and prognosis

Tumor circulome in the liquid biopsies for cancer diagnosis and prognosis | from Flow Cytometry to Cytomics | Scoop.it
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Analyzing Myeloid Cells Using Flow Cytometry

Analyzing Myeloid Cells Using Flow Cytometry | from Flow Cytometry to Cytomics | Scoop.it
A group has outlined a protocol for the tissue-resident myeloid populations in mouse tissues using flow cytometry.
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Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report | American Journal of Respiratory Cell and Molecular Biology

Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report | American Journal of Respiratory Cell and Molecular Biology | from Flow Cytometry to Cytomics | Scoop.it
Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive metho...
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Immune profiling of influenza‐specific B cell and T cell responses in macaques using flow cytometry‐based assays - Koutsakos - - Immunology & Cell Biology

Immune profiling of influenza‐specific B cell and T cell responses in macaques using flow cytometry‐based assays - Koutsakos - - Immunology & Cell Biology | from Flow Cytometry to Cytomics | Scoop.it
Influenza remains a significant global public health burden, despite substantial annual vaccination efforts against circulating virus strains. As a result, novel vaccine approaches are needed t
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Cells | Free Full-Text | Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells

Cells | Free Full-Text | Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells | from Flow Cytometry to Cytomics | Scoop.it
In the last few decades, the epithelial cell adhesion molecule (EpCAM) has received increased attention as the main membrane marker used in many enrichment technologies to isolate circulating tumor cells (CTCs). Although there has been a great deal of progress in the implementation of EpCAM-based CTC detection technologies in medical settings, several issues continue to limit their clinical utility. The biology of EpCAM and its role are not completely understood but evidence suggests that the expression of this epithelial cell-surface protein is crucial for metastasis-competent CTCs and may not be lost completely during the epithelial-to-mesenchymal transition. In this review, we summarize the most significant advantages and disadvantages of using EpCAM as a marker for CTC enrichment and its potential biological role in the metastatic cascade.
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Frontiers | A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue | Immunology

Frontiers | A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue | Immunology | from Flow Cytometry to Cytomics | Scoop.it
Imaging mass cytometry (IMC) is able to quantify the expression of dozens of markers at sub-cellular resolution on a single tissue section by combining a novel laser ablation system with mass cytometry. As such, it allows us to gain spatial information and antigen quantification in situ, and can be applied to both snap-frozen and formalin-fixed, paraffin-embedded (FFPE) tissue sections. Herein, we have developed and optimized the immunodetection conditions for a 34-antibody panel for use on human snap-frozen tissue sections. For this, we tested the performance of 80 antibodies. Moreover, we compared tissue drying times, fixation procedures and antibody incubation conditions. We observed that variations in the drying times of tissue sections had little impact on the quality of the images. Fixation with methanol for 5 min at −20°C or 1% paraformaldehyde (PFA) for 5 min at room temperature followed by methanol for 5 min at −20°C were superior to fixation with acetone or PFA only. Finally, we observed that antibody incubation overnight at 4°C yielded more consistent results as compared to staining at room temperature for 5 h. Finally, we used the optimized method for staining of human fetal and adult intestinal tissue samples. We present the tissue architecture and spatial distribution of the stromal cells and immune cells in these samples visualizing blood vessels, the epithelium and lamina propria based on the expression of α-smooth muscle actin (α-SMA), E-Cadherin an
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Mass cytometry reveals immune signatures associated with cytomegalovirus (CMV) control in recipients of allogeneic haemopoietic stem cell transplant and CMV‐specific T cells - McGuire - 2020 - Clin...

Mass cytometry reveals immune signatures associated with cytomegalovirus (CMV) control in recipients of allogeneic haemopoietic stem cell transplant and CMV‐specific T cells - McGuire - 2020 - Clin... | from Flow Cytometry to Cytomics | Scoop.it
In this study, immune profiling with mass cytometry identified immune signatures associated with cytomegalovirus (CMV) reactivation after allogeneic stem cell transplant in patients undergoin
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Circulating tumour cells as a potential biomarker for lung cancer screening: a prospective cohort study - ScienceDirect

Lung cancer screening with low-dose chest CT (LDCT) reduces the mortality of eligible individuals. Blood signatures might act as a standalone screenin…
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negative results

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DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells - Di Trapani - 2018 - Cytometry Part A

DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells - Di Trapani - 2018 - Cytometry Part A | from Flow Cytometry to Cytomics | Scoop.it
Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTC
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Methodology for in vitro Assessment of Human T Cell ...

Methodology for in vitro Assessment of Human T Cell ... | from Flow Cytometry to Cytomics | Scoop.it
Methods to test both the functionality and mechanism of action for human recombinant proteins and antibodies in vitro have been limited by multiple factors. To test the functionality of a recombinant protein or antibody, the receptor, the receptor-associated ligand, or both must be expressed by...
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Clinical Utility of Flow Cytometric Leukocyte Subset Identification

Clinical Utility of Flow Cytometric Leukocyte Subset Identification | from Flow Cytometry to Cytomics | Scoop.it
The common perception is that circulating blood cells are composed of red and white cells. In reality, the “white cell” population (leukocytes) contains many different cell types.
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Programme de DPC 2020 • Société Française d'Immunologie

Programme de DPC 2020 • Société Française d'Immunologie | from Flow Cytometry to Cytomics | Scoop.it
Programme DPC n°50982000002
Gilbert C FAUREs insight:

pas covid, flow cytometry et basophiles en allergie

 

autres ressources sur ces pages

https://www.scoop.it/topic/from-flow-cytometry-to-cytomics?q=basophil

 

Gilbert C FAURE's curator insight, October 12, 7:24 AM

centré allergologie et cytométrie de flux, pas covid-19

Gilbert C FAURE's curator insight, October 12, 7:24 AM

pas covid-19, basophiles et cytométrie

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Telomeres and Aging | Physiological Reviews

Telomeres play a central role in cell fate and aging by adjusting the cellular response to stress and growth stimulation on the basis of previous cell divisions and DNA damage. At least a few hundr...
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Detection of Rare Cells by Flow Cytometry: a step-by-step guide | Thermo Fisher Scientific - FR

Home › Detection of Rare Cells by Flow Cytometry: a step-by-step guide Detection of Rare Cells by Flow Cytometry: a step-by-step guide
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Flow Cytometry Applications in Infectious Disease | Flow Metrics

Flow Cytometry Applications in Infectious Disease | Flow Metrics | from Flow Cytometry to Cytomics | Scoop.it
Technological advances have resulted in the development of many streamlined flow cytometry systems for field medicine applications to tackle global challenges....
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Overview of research on circulating tumor cells

Overview of research on circulating tumor cells | from Flow Cytometry to Cytomics | Scoop.it
Get an overview of circulating tumor cells, find relevant papers, and identify important concepts.
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Challenges in circulating tumor cell detection by the CellSearch system - ScienceDirect

Challenges in circulating tumor cell detection by the CellSearch system - ScienceDirect | from Flow Cytometry to Cytomics | Scoop.it
Enumeration and characterization of circulating tumor cells (CTC) hold the promise of a real time liquid biopsy. They are however present in a large b…
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Frontiers | The Metastatic Cascade as the Basis for Liquid Biopsy Development | Oncology

Frontiers | The Metastatic Cascade as the Basis for Liquid Biopsy Development | Oncology | from Flow Cytometry to Cytomics | Scoop.it
The metastatic cascade describes the process whereby aggressive cancer cells leave the primary tumor, travel through the bloodstream, and eventually reach distant organs to develop one or several metastases. During the last decade, innovative technologies have exploited the recent biological knowledge to identify new circulating biomarkers for the screening and early detection of cancer, real-time monitoring of treatment response, assessment of tumor relapse risk (prognosis), identification of new therapeutic targets and resistance mechanisms, patient stratification, and therapeutic decision-making. These techniques are broadly described using the term of Liquid Biopsy. This field is in constant progression and is based on the detection of circulating tumor cells, circulating free nucleic acids (e.g., circulating tumor DNA), circulating tumor-derived extracellular vesicles, and tumor-educated platelets. The aim of this review is to describe the biological principles underlying the liquid biopsy concept and to discuss how functional studies can expand the clinical applications of these circulating biomarkers.
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Predicting Metastasis in Melanoma by Enumerating Circulating Tumor Cells Using Photoacoustic Flow Cytometry - Edgar - - Lasers in Surgery and Medicine

Predicting Metastasis in Melanoma by Enumerating Circulating Tumor Cells Using Photoacoustic Flow Cytometry - Edgar - - Lasers in Surgery and Medicine | from Flow Cytometry to Cytomics | Scoop.it
Background and Objectives Enumerating circulating tumor cells has been used as a method of monitoring progression of various cancers. Various methods for detecting circulating melanoma cells (CMCs)...
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Circulating tumour cells as a potential biomarker for lung cancer screening: a prospective cohort study

Circulating tumour cells as a potential biomarker for lung cancer screening: a prospective cohort study | from Flow Cytometry to Cytomics | Scoop.it
CTC detection using ISET is not suitable for lung cancer screening.
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Molecular characterization of circulating tumour cells identifies predictive markers for outcome in primary, triple‐negative breast cancer patients - Bittner - - Journal of Cellular and Molecular M...

Molecular characterization of circulating tumour cells identifies predictive markers for outcome in primary, triple‐negative breast cancer patients - Bittner - - Journal of Cellular and Molecular M... | from Flow Cytometry to Cytomics | Scoop.it
Abstract mRNA profiles of circulating tumour cells (CTCs) were analysed in patients with triple‐negative breast cancer (TNBC) (pts) before (BT) and after therapy (AT) to identify additional treatme...
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Cerebrospinal Fluid Circulating Tumor Cells as a Quantifiable Measurement of Leptomeningeal Metastases in Patients With HER2 Positive Cancer

Clinicaltrials.gov: NCT01325207; registered March 29th, 2011.
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