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Rescooped by Kalai Ponmani from Ag Biotech News
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Genetic Engineering of Horticultural Crops - Elsevier (2018) 

Genetic Engineering of Horticultural Crops provides key insights into commercialized crops, their improved productivity, disease and pest resistance, and enhanced nutritional or medicinal benefits... Modern biotechnology has helped to increase crop productivity by introducing novel gene(s) with high quality disease resistance and increased drought tolerance... 


For five decades conventional plant breeding techniques have made considerable progress in the development of improved varieties including horticultural crops. However, it has not been able to keep pace with the increasing demand for vegetables and fruits in developing countries. Therefore an instant need is felt to incorporate modern recombinant DNA technology to speed up crop improvement programs. 


Recombinant DNA technology has offered wonderful scope and potential to conventional methods of crop improvement, crop protection, and crop quality management. Transgenic technology in horticultural crops is helpful in increasing crop production and productivity, managing abiotic and biotic stress, enhancing nutritional value, and increasing shelf-life. This is why the global land area occupied by genetically modified crops has been continuously increasing during the last two decades, and reached a record 182 million ha globally by 2014... 


The world faces an invisible health crisis in the form of hidden hunger and micronutrient deficiency, and the concomitant global demand for food is rising continuously. The burgeoning population needs crops engineered to produce more and tolerate climate change. Genetic engineering has revolutionized the entire crop improvement programs by providing crops with improved nutritional value, biotic and abiotic stress tolerance, therapeutic and industrial proteins, and superior agronomic traits. 


In the last decade, genome editing with designer nucleases has had a great impact on crop breeding as well as on human lives... We attempt to summarize the transgenic technologies and their potential applications in the improvement of horticultural crops. The future challenges and opportunities for the deployment of genetic engineering and genome editing technologies in horticultural crop improvements are also discussed... 


Crop improvement in terms of high yield and tolerance to biotic and abiotic stresses can be easily achieved by supplementing conventional breeding with modern biotechnological techniques such as “transgenics.” Furthermore, generating transgenic crops is a more specific and faster approach for crop improvement. A wide variety of transformation and regeneration protocols are available, which have resulted in engineering a wide array of horticultural crops for pest and disease resistance, enhanced shelf-life, etc. 


The issues of public concern are use of selectable marker genes, spread of the transgenes through pollen, possibility for the development of resistant lines in case of insect or fungal pests, allergenicity of the introduced proteins in humans, etc. Techniques are available to eliminate the use of selectable markers in genetic transformation... Numbers of research groups and reputed organizations have assessed the state-of-the-art with respect to the safety of genetically modified (GM) plants for human consumption and also concluded that transgenic crop varieties are as safe and nutritious as their respective non-GM plants. 


Regulatory issues pose a significant obstacle to the development of transgenics and their commercialization. Efforts should be made to hasten the conduct of necessary toxicological studies and issue of necessary clearance to the transgenic crop varieties. Advanced genome editing techniques such as ZFN, TALEN, and CRISPR/Cas9 have opened more avenues for the development of genetically edited isogenic lines, which are both consumer and environmentally friendly. Furthermore, they do not warrant much regulation because they are near-isogenic lines of the parental types... 


https://www.sciencedirect.com/science/book/9780128104392



Via Alexander J. Stein
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Efficient TALEN construction and evaluation methods for human cell and animal applications - Sakuma - 2013 - Genes to Cells - Wiley Online Library

Efficient TALEN construction and evaluation methods for human cell and animal applications - Sakuma - 2013 - Genes to Cells - Wiley Online Library | TALENS | Scoop.it
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Quantitative analysis of TALE–DNA interactions suggests polarity effects

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PLOS Computational Biology: Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites

PLOS Computational Biology: Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites | TALENS | Scoop.it
PLOS Computational Biology is an open-access
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Genome editing: That's the way the CRISPR crumbles : Nature : Nature Research

Nathaniel Comfort finds heroism but little nuance in Jennifer Doudna's account of her co-discovery.
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BMC Bioinformatics | Full text | Mojo Hand, a TALEN design tool for genome editing applications

Recent studies of transcription activator-like (TAL) effector domains fused to nucleases (TALENs) demonstrate enormous potential for genome editing.
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Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases

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Cold Spring Harbor Symposium on Quantitative Biology: Effector Biology of Plant-Associated Organisms: Concepts and Perspectives (2012)

Cold Spring Harbor Symposium on Quantitative Biology: Effector Biology of Plant-Associated Organisms: Concepts and Perspectives (2012) | TALENS | Scoop.it

Every plant is closely associated with a variety of living organisms. Therefore, deciphering howplants interact with mutualistic and parasitic organisms is essential for a comprehensive understanding of the biology of plants. The field of plant–biotic interactions has recently coalesced around an integrated model. Major classes of molecular players both from plants and their associated organisms have been revealed. These include cell surface and intracellular immune receptors of plants, as well as apoplastic and host-cell translocated (cytoplasmic) effectors of the invading organism. This article focuses on effectors, molecules secreted by plant-associated organisms that alter plant processes. Effectors have emerged as a central class of molecules in our integrated view of plant–microbe interactions. Their study has significantly contributed to advancing our knowledge of plant hormones, plant development, plant receptors, and epigenetics. Many pathogen effectors are extraordinary examples of biological innovation; they include some of themost remarkable proteins knownto function inside plant cells. Here, we review some of the key concepts that have emerged from the study of the effectors of plant-associated organisms. In particular, we focus on how effectors function in plant tissues and discuss future perspectives in the field of effector biology.

 

http://kamounlab.dreamhosters.com/pdfs/CSHSQB_2012.pdf


Via Kamoun Lab @ TSL
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