Stem Cells & Tissue Engineering
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Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury

Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper in PNAS describes a novel tissue engineering method to repair soft tissue defects. Researchers from the lab of Prof. Shulamit Levenberg in the Technion, lead by Dr. Yulia Shandalov have fabricated an engineered muscle flap bearing its own functional vascular pedicle, and used it to repair a large soft tissue defect in mice. First, a 3D scaffold seeded with endothelial cells, fibroblasts, and myoblasts was prepared in-vitro. Then, it was implanted in-vivo around the femoral artery and veins before it was transferred to reconstruct an abdominal wall defect in the same mouse.

Read the full paper: 

http://www.pnas.org/content/early/2014/04/02/1402679111.long


* I was part of the Levenberg's lab between the years 2010-2012.



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Rejuvenation of the muscle stem cell population restores strength to injured aged muscles - Nature Medicine

Jacob Blumenthal's insight:

A new paper, published in Nature Medicine suggests that in-vitro modification of skeletal muscle stem cells (MuSCs) isolated from aged mice, can restore their potential for regeneration  and their ability to strengthen damaged muscles, when transplanted back into aged mice.

Full paper:

http://www.nature.com/nm/journal/vaop/ncurrent/full/nm.3464.html

 

Media release: http://media.utoronto.ca/media-releases/turning-back-the-clock-on-aging-muscles/

 

Learn more about the embryonic development of skeletal muscles:

http://discovery.lifemapsc.com/in-vivo-development/skeletal-muscle

 

 

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Isolation, characterization, and molecular regulation of muscle stem cells | Frontiers in Striated Muscle Physiology

Isolation, characterization, and molecular regulation of muscle stem cells | Frontiers in Striated Muscle Physiology | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

This open access review describes a method for direct  isolation of muscle stem cells also known as satellite cells. In addition, it details their characteristics and their unique gene expression profile.

 

To learn more about the embryonic development of skeletal muscles, and related stem cells differentiation protocols:

http://discovery.lifemapsc.com/in-vivo-development/skeletal-muscle

 

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Cell Stem Cell - Myf5-Positive Satellite Cells Contribute to Pax7-Dependent Long-Term Maintenance of Adult Muscle Stem Cells

Cell Stem Cell - Myf5-Positive Satellite Cells Contribute to Pax7-Dependent Long-Term Maintenance of Adult Muscle Stem Cells | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Researchers from the Max-Planck institute and Carnegie Institution of Washington found that induced inactivation of Pax7 in Pax7-expressing cells of adult mice leads to loss of muscle stem cells and reduced heterochromatin condensation in rare surviving satellite cells. Inactivation of Pax7 in Myf5-expressing cells revealed that the majority of adult muscle stem cells originate from myogenic lineages, which express the myogenic regulators Myf5 or MyoD. Likewise, the majority of muscle stem cells are replenished from Myf5-expressing myogenic cells during adult life, and inactivation of Pax7 in Myf5-expressing cells after muscle damage leads to a complete arrest of muscle regeneration. The authors also  demonstrate that a relatively small number of muscle stem cells are sufficient for efficient repair of skeletal muscles. They conclude that Pax7 acts at different levels in a nonhierarchical regulatory network controlling muscle-satellite-cell-mediated muscle regeneration.

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To learn about skeletal muscle embryonic development :

http://discovery.lifemapsc.com/in-vivo-development/skeletal-muscle


For skeletal muscle-related, stem cells differentiation protocols:

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols


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Jan-Jan Liu's curator insight, August 8, 2013 10:15 PM

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Stem cell advance yields mature heart muscle cells

Stem cell advance yields mature heart muscle cells | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Using micropatterned 3D substrates, researchers from UW-Madison were able to align stem cell-derived cardiomyocytes and to form a sarcomere structure.

http://www.sciencedirect.com/science/article/pii/S0142961214001276


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Pluristem stem cell trial to treat muscle injury meets main goal | Reuters

Pluristem stem cell trial to treat muscle injury meets main goal | Reuters | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Pluristem had announced that their placenta-derived stem cell product showed promising results in a clinical trial for the treatment of muscle injury.

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Cell - A Zebrafish Embryo Culture System Defines Factors that Promote Vertebrate Myogenesis across Species

Cell - A Zebrafish Embryo Culture System Defines Factors that Promote Vertebrate Myogenesis across Species | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A team of researchers, led by Leonard Zon, Amy Wagers, and Ronald Kahn describe a novel technique to identify small molecules and chemicals that can promote myogenesis differentiation and proliferation. The main goal of the study was to identify novel molecules that will allow efficient skeletal muscle differentiation of induced pluripotent stem (iPS) cells. These skeletal muscle cells, could be then transplanted into patients with muscle loss.

In order to achieve that, the researchers first use a zebrafish embryo-based culture system to screen for molecules that promote myogenesis and expansion of muscle progenitors. Then they applied these molecules on iPS cells, to generate engraftable myogenic progenitors that contributed to muscle repair in vivo.

http://www.cell.com/abstract/S0092-8674(13)01299-3

 

To learn about skeletal muscle embryonic development :

http://discovery.lifemapsc.com/in-vivo-development/skeletal-muscle

 

*The picture is taken from Lifemap Discovery database*

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Scientists discern signatures of old versus young stem cells

Scientists discern signatures of old versus young stem cells | Stem Cells & Tissue Engineering | Scoop.it
A chemical code scrawled on histones -- the protein husks that coat DNA in every animal or plant cell -- determines which genes in that cell are turned on and which are turned off.
Jacob Blumenthal's insight:

In a study published June 27 in Cell Reports, the researchers explored the chromatin features of adult skeletal muscle stem cells from young and old mice. By comparing the epigenetic profiles of these cells, the authors provided a direct evidence that, with age, epigenetic changes accumulate and may lead to a functional decline in quiescent stem cells. Their findings highlight the importance of chromatin mapping in understanding the  unique features of stem cell identity and stem cell aging.

http://www.cell.com/cell-reports/fulltext/S2211-1247(13)00276-3

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