RamaScience
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RamaScience
On my scientific interest, mainly plant science
Curated by Ramiro Paris
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Functions of S-nitrosylation in plant hormone networks | Frontiers in Plant Physiology

Functions of S-nitrosylation in plant hormone networks | Frontiers in Plant Physiology | RamaScience | Scoop.it
In plants, a wide frame of physiological processes is regulated in liaison by both, nitric oxide (NO) and hormones. Such overlapping roles raise the question of how is the cross-talk between NO and hormones to trigger common physiological responses.
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Nature destaca el crecimiento de la ciencia argentina - Ministerio de Ciencia, Tecnología e Innovación Productiva

Nature destaca el crecimiento de la ciencia argentina - Ministerio de Ciencia, Tecnología e Innovación Productiva | RamaScience | Scoop.it
La prestigiosa revista dedica su edición de junio al desarrollo científico en América del Sur. Argentina y Brasil encabezan las investigaciones colaborativas.
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Plant sugars are crucial players in the oxidative challenge during abiotic stress: extending the traditional concept - KEUNEN - 2013 - Plant, Cell & Environment - Wiley Online Library

Plant sugars are crucial players in the oxidative challenge during abiotic stress: extending the traditional concept - KEUNEN - 2013 - Plant, Cell & Environment - Wiley Online Library | RamaScience | Scoop.it
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¿Y si la ciencia no es eso que tú crees?

¿Y si la ciencia no es eso que tú crees? | RamaScience | Scoop.it
El último Nobel de Medicina critica la “tiranía” de las revistas especializadas en la carrera investigadora. Las publicaciones defienden su seriedad
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The structural basis of specific protease–inhibitor interactions at the plant–pathogen interface

The structural basis of specific protease–inhibitor interactions at the plant–pathogen interface | RamaScience | Scoop.it
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Nietzsche

Nietzsche | RamaScience | Scoop.it
Before you get angry about misrepresenting Nietzsche, please remember THIS IS A COMIC STRIP.My sci-fi thriller, LAZARUS, is out on Smashwords and Amazon! Yeah, I guess there's some Nietzsche in the...
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A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene

A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene | RamaScience | Scoop.it
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Science: Pivoting the Plant Immune System from Dissection to Deployment (2013)

Science: Pivoting the Plant Immune System from Dissection to Deployment (2013) | RamaScience | Scoop.it

Diverse and rapidly evolving pathogens cause plant diseases and epidemics that threaten crop yield and food security around the world. Research over the last 25 years has led to an increasingly clear conceptual understanding of the molecular components of the plant immune system. Combined with ever-cheaper DNA-sequencing technology and the rich diversity of germ plasm manipulated for over a century by plant breeders, we now have the means to begin development of durable (long-lasting) disease resistance beyond the limits imposed by conventional breeding and in a manner that will replace costly and unsustainable chemical controls.


Via Suayib Üstün, Nicolas Denancé
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Nature Biotech: Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease (2013)

Nature Biotech: Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease (2013) | RamaScience | Scoop.it

http://www.nature.com/nbt/journal/v31/n8/full/nbt.2655.html

 

Sustainable intensification of crop production is essential to ensure food demand is matched by supply as the human population continues to increase. This will require high-yielding crop varieties that can be grown sustainably with fewer inputs on less land. Both plant breeding and genetic modification (GM) methods make valuable contributions to varietal improvement, but targeted genome engineering promises to be critical to elevating future yields. Most such methods require targeting DNA breaks to defined locations followed by either nonhomologous end joining (NHEJ) or homologous recombination. Zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be engineered to create such breaks, but these systems require two different DNA binding proteins flanking a sequence of interest, each with a C-terminal FokI nuclease module. We report here that the bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR) system, comprising a CRISPR-associated (Cas)9 protein and an engineered single guide RNA (sgRNA) that specifies a targeted nucleic acid sequence, is applicable to plants to induce mutations at defined loci.


Via Kamoun Lab @ TSL, Suayib Üstün
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Elizabeth Jones's curator insight, October 2, 2013 4:25 PM

CRISPR-Cas9 is hot right now, and it's being used in several different ways: for genome-editing in cells of various species (here they're showing it works in plants),  or to create heritable changes (i.e. new transgenic animal lines), and also as a transcription factor to regulate gene expression without altering the nuclear DNA. 

 

Constructs that use Cas9 and synthetic guide RNA (sgRNA) can be customized to target any gene of interest, and can produce insertions, deletions, or incorportation of variant sequences into genomic DNA. Customized constructs can be ordered from gene synthesis suppliers such as GenScript, and delivered much more quickly and cheaply than they can be produced in a typical lab using traditional molecular cloning techniques. 

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The frustrated science student behind Sci-Hub

The frustrated science student behind Sci-Hub | RamaScience | Scoop.it
Alexandra Elbakyan founded Sci-Hub to thwart journal paywalls
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Science in the Classroom | A collection of annotated research papers and accompanying teaching materials

Science in the Classroom | A collection of annotated research papers and accompanying teaching materials | RamaScience | Scoop.it
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10 types of scientist | www.sciencecouncil.org

10 types of scientist | www.sciencecouncil.org | RamaScience | Scoop.it
Diana Garnham identifies 10 types of scientist.
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Por qué revistas como ‘Nature’, ‘Science’ y ‘Cell’ hacen daño a la ciencia

Por qué revistas como ‘Nature’, ‘Science’ y ‘Cell’ hacen daño a la ciencia | RamaScience | Scoop.it
El premio Nobel protesta contra el sistema de publicaciones en el mundo de la investigación
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Worldviews

Worldviews | RamaScience | Scoop.it
My sci-fi thriller, LAZARUS, is out on Smashwords and Amazon! Follow The Upturned Microscope on Twitter and Facebook!
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Simultaneous application of heat, drought and virus to Arabidopsis thaliana plants reveals significant shifts in signaling networks

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Diariodeciencias.com.ar | Ciencias - Tecnologia - Produccion

Diariodeciencias.com.ar | Ciencias - Tecnologia - Produccion | RamaScience | Scoop.it
Ciencias – Tecnologia – Produccion
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Unhelpful Research Advice #4

Unhelpful Research Advice #4 | RamaScience | Scoop.it
My sci-fi thriller, LAZARUS, is out on both Smashwords and Amazon! Because of making books, there is no end. Follow The Upturned Microscope on Twitter and Facebook. We can't all meet up for coffee....
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Metaphor

Metaphor | RamaScience | Scoop.it
My sci-fi thriller, LAZARUS, is out on both Smashwords and Amazon! It is guaranteed to cause a 360-degree change in your life. Follow The Upturned Microscope on Twitter and Facebook. All the cool k...
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Lab management

Lab management | RamaScience | Scoop.it
More management My sci-fi thriller, LAZARUS, is now out on both Smashwords and Amazon. Help out an indie novelist, and the indie fairy will leave you an indie blessing under your indie pillow. And ...
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PLOS Pathogens: The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence

PLOS Pathogens: The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence | RamaScience | Scoop.it

The phytopathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) requires type III effector proteins (T3Es) for virulence. After translocation into the host cell, T3Es are thought to interact with components of host immunity to suppress defence responses. XopJ is a T3E protein from Xcv that interferes with plant immune responses; however, its host cellular target is unknown. Here we show that XopJ interacts with the proteasomal subunit RPT6 in yeast andin planta to inhibit proteasome activity. A C235A mutation within the catalytic triad of XopJ as well as a G2A exchange within the N-terminal myristoylation motif abolishes the ability of XopJ to inhibit the proteasome. Xcv ΔxopJ mutants are impaired in growth and display accelerated symptom development including tissue necrosis on susceptible pepper leaves. Application of the proteasome inhibitor MG132 restored the ability of the Xcv ΔxopJ to attenuate the development of leaf necrosis. The XopJ dependent delay of tissue degeneration correlates with reduced levels of salicylic acid (SA) and changes in defence- and senescence-associated gene expression. Necrosis upon infection with Xcv ΔxopJ was greatly reduced in pepper plants with reduced expression of NPR1, a central regulator of SA responses, demonstrating the involvement of SA-signalling in the development of XopJ dependent phenotypes. Our results suggest that XopJ-mediated inhibition of the proteasome interferes with SA-dependent defence response to attenuate onset of necrosis and to alter host transcription. A central role of the proteasome in plant defence is discussed.


Via Suayib Üstün
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Suayib Üstün's comment, June 14, 2013 3:01 AM
thank you so much! I'm open for questions, criticism and discussion!
CP's comment, June 14, 2013 9:14 AM
Congrats again!!
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The structural basis of specific protease–inhibitor interactions at the plant–pathogen interface

The structural basis of specific protease–inhibitor interactions at the plant–pathogen interface | RamaScience | Scoop.it

Antagonistic host–pathogen interactions offer intriguing insights into coevolutionary processes at the molecular level. Studies on secreted immune proteases from the model plant tomato and their interactions with different unrelated pathogen-derived inhibitors revealed that the inhibitors exhibit a remarkable selectivity towards different host proteases, and that the host proteases accumulate variant residues at the interaction surfaces that interfere with inhibitor binding. Here, we summarize and discuss the recent findings and use structural models to identify the molecular features underpinning protease selectivity. The observed basic principles translate to other examples of secreted immune hydrolases and their putative inhibitors.


Via Suayib Üstün
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