Protocols and methods
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JoVE Video: High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

JoVE Video: High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae | Protocols and methods | Scoop.it
In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries.
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JoVE Video: Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

JoVE Video: Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration | Protocols and methods | Scoop.it
Behavior assays for measuring locomotor functions, learning, and memory abilities in Drosophila.
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JoVE Video: Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

JoVE Video: Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos | Protocols and methods | Scoop.it
We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis.
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JoVE Video: Calcium Imaging of Cortical Neurons using Fura-2 AM

JoVE Video: Calcium Imaging of Cortical Neurons using Fura-2 AM | Protocols and methods | Scoop.it
Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM.
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JoVE Video: Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

JoVE Video: Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae | Protocols and methods | Scoop.it
Here we describe electrophysiological methods for measuring synaptic transmission at the neuromuscular junction of Drosophila larva.
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JoVE Video: Dissection and Staining of Drosophila Larval Ovaries

JoVE Video: Dissection and Staining of Drosophila Larval Ovaries | Protocols and methods | Scoop.it
How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development.
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JoVE Video: Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

JoVE Video: Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging | Protocols and methods | Scoop.it
This protocol describes three Drosophila preparations: 1) adult brain dissection, 2) adult retina dissection and 3) developing eye disc- brain complexes dissection.
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JoVE Video: Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

JoVE Video: Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction | Protocols and methods | Scoop.it
The neuromuscular junction (NMJ) of Drosophila melanogaster is an important model system for studying normal synaptic function as well as perturbations to synaptic function found in certain neurological diseases.
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JoVE Video: Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

JoVE Video: Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae | Protocols and methods | Scoop.it
In this article, we demonstrate assays to study thermal nociception in Drosophila larvae.
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JoVE Video: Methods to Assay Drosophila Behavior

JoVE Video: Methods to Assay Drosophila Behavior | Protocols and methods | Scoop.it
Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1.
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JoVE Video: In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee

JoVE Video: In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee | Protocols and methods | Scoop.it
Bees can be conditioned in an appetitive olfactory learning paradigm (PER-conditioning).
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JoVE Video: Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions

JoVE Video: Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions | Protocols and methods | Scoop.it
This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae.
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JoVE Video: Drosophila Larval NMJ Dissection

JoVE Video: Drosophila Larval NMJ Dissection | Protocols and methods | Scoop.it
This protocol demonstrates how to dissect Drosophila larvae in preparation for immunohistochemistry and/or imaging of the neuromuscular junction.
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JoVE Video: Dissection of Larval CNS in Drosophila Melanogaster

JoVE Video: Dissection of Larval CNS in Drosophila Melanogaster | Protocols and methods | Scoop.it
In this article we demonstrate how to dissect the central nervous system from third instar Drosophila larvae.
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