Researches for plant immnunity
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Rescooped by Yue-peng Zang from Trends in MPMI
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The Tomato Fni3 Lysine-63–Specific Ubiquitin-Conjugating Enzyme and Suv Ubiquitin E2 Variant Positively Regulate Plant Immunity

The Tomato Fni3 Lysine-63–Specific Ubiquitin-Conjugating Enzyme and Suv Ubiquitin E2 Variant Positively Regulate Plant Immunity | Researches for plant immnunity | Scoop.it

The activation of an immune response in tomato (Solanum lycopersicum) against Pseudomonas syringae relies on the
recognition of E3 ligase–deficient forms of AvrPtoB by the host protein kinase, Fen. To investigate the mechanisms by which
Fen-mediated immunity is regulated, we characterize in this study a Fen-interacting protein, Fni3, and its cofactor,
S. lycoperiscum Uev (Suv). Fni3 encodes a homolog of the Ubc13-type ubiquitin-conjugating enzyme that catalyzes
exclusively Lys-63–linked ubiquitination, whereas Suv is a ubiquitin-conjugating enzyme variant. The C-terminal region of Fen
was necessary for interaction with Fni3, and this interaction was required for cell death triggered by overexpression of Fen in
Nicotiana benthamiana leaves. Fni3 was shown to be an active E2 enzyme, but Suv displayed no ubiquitin-conjugating
activity; Fni3 and Suv together directed Lys-63–linked ubiquitination. Decreased expression of Fni3, another tomato Ubc13
homolog, Sl-Ubc13-2, or Suv in N. benthamiana leaves diminished cell death associated with Fen-mediated immunity and cell
death elicited by several other resistance (R) proteins and their cognate effectors. We also discovered that coexpression of
Fen and other R proteins/effectors with a Fni3 mutant that is compromised for ubiquitin-conjugating activity diminished the
cell death. These results suggest that Fni3/Sl-Ubc13-2 and Suv regulate the immune response mediated by Fen and other R
proteins through Lys-63–linked ubiquitination.


Via Suayib Üstün, CP
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Sheng Yang He Lab at Michigan State University

Sheng Yang He Lab at Michigan State University | Researches for plant immnunity | Scoop.it

The Sheng Yang He Lab at Michigan State University welcomes you! Our research focuses on the molecular biology of host-microbe interactions, microbial pathogenesis, and hormone signaling in disease.

 

Sheng-Yang is also a HHMI/GBMF Plant Biology Investigator http://www.hhmi.org/news/he_bg.html


Via Kamoun Lab @ TSL, Yue-peng Zang
Yue-peng Zang's insight:

Plant-microbe interaction

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The U-Box E3 Ligase SPL11/PUB13 Is a Convergence Point of Defense and Flowering Signaling in Plants

The U-Box E3 Ligase SPL11/PUB13 Is a Convergence Point of Defense and Flowering Signaling in Plants | Researches for plant immnunity | Scoop.it

Via CP
Yue-peng Zang's insight:
Ubiquitination and plant immunity
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Rescooped by Yue-peng Zang from Plants and Microbes
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Sheng Yang He Lab at Michigan State University

Sheng Yang He Lab at Michigan State University | Researches for plant immnunity | Scoop.it

The Sheng Yang He Lab at Michigan State University welcomes you! Our research focuses on the molecular biology of host-microbe interactions, microbial pathogenesis, and hormone signaling in disease.

 

Sheng-Yang is also a HHMI/GBMF Plant Biology Investigator http://www.hhmi.org/news/he_bg.html


Via Kamoun Lab @ TSL
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Yue-peng Zang's curator insight, August 16, 2013 3:21 AM

Plant-microbe interaction