Molecular regulation of fruit development and ripeing
9 views | +0 today
Follow
Your new post is loading...
Your new post is loading...
Scooped by Xiaoming Pang
Scoop.it!

Natural occurring epialleles determine vitamin E accumulation in tomato fruits

Vitamin E (VTE) content is a low heritability nutritional trait for which the genetic determinants are poorly understood. Here, we focus on a previously detected major tomato VTE quantitative trait loci (QTL; mQTL9-2-6) and identify the causal gene as one encoding a 2-methyl-6-phytylquinol methyltransferase (namely VTE3(1)) that catalyses one of the final steps in the biosynthesis of γ- and α-tocopherols, which are the main forms of VTE. By reverse genetic approaches, expression analyses, siRNA profiling and DNA methylation assays, we demonstrate that mQTL9-2-6 is an expression QTL associated with differential methylation of a SINE retrotransposon located in the promoter region of VTE3(1). Promoter DNA methylation can be spontaneously reverted leading to different epialleles affecting VTE3(1) expression and VTE content in fruits. These findings indicate therefore that naturally occurring epialleles are responsible for regulation of a nutritionally important metabolic QTL and provide direct evidence of a role for epigenetics in the determination of agronomic traits.

more...
No comment yet.
Scooped by Xiaoming Pang
Scoop.it!

Ohio State researchers clone a gene that regulates fruit size in vegetable ... - Delphos Herald

Ohio State researchers clone a gene that regulates fruit size in vegetable ... - Delphos Herald | Molecular regulation of fruit development and ripeing | Scoop.it
Ohio State researchers clone a gene that regulates fruit size in vegetable ...
Delphos Herald
The cloned gene, known as SlKLUH, impacts fruit size by increasing cell layers and delaying ripening.
more...
No comment yet.
Scooped by Xiaoming Pang
Scoop.it!

Detection of genomic variations and DNA polymorphisms and impact on analysis of meiotic recombination and genetic mapping

Detection of genomic variations and DNA polymorphisms and impact on analysis of meiotic recombination and genetic mapping | Molecular regulation of fruit development and ripeing | Scoop.it

DNA polymorphisms are important markers in genetic analyses and are increasingly detected by using genome resequencing. However, the presence of repetitive sequences and structural variants can lead to false positives in the identification of polymorphic alleles. Here, we describe an analysis strategy that minimizes false positives in allelic detection and present analyses of recently published resequencing data from Arabidopsis meiotic products and individual humans. Our analysis enables the accurate detection of sequencing errors, small insertions and deletions (indels), and structural variants, including large reciprocal indels and copy number variants, from comparisons between the resequenced and reference genomes. We offer an alternative interpretation of the sequencing data of meiotic products, including the number and type of recombination events, to illustrate the potential for mistakes in single-nucleotide polymorphism calling. Using these examples, we propose that the detection of DNA polymorphisms using resequencing data needs to account for nonallelic homologous sequences.

more...
No comment yet.