Miscelâneas da Biotecnologia & Bioengenharia
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Rescooped by Léo Felippe Dias from Bioinformatics Software: Sequence Analysis
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Identifying and correcting errors in draft genomes

Identifying and correcting errors in draft genomes | Miscelâneas da Biotecnologia & Bioengenharia | Scoop.it
Over the past decade we have seen an exponential increase in the number of sequenced, assembled, and annotated genomes. These these genomes are essential for pretty much any genomics research. If y...

Via Mel Melendrez-Vallard
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Scooped by Léo Felippe Dias
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GTPB: BPB14 Bioinformatics using Python for Biologists

GTPB: BPB14 Bioinformatics using Python for Biologists | Miscelâneas da Biotecnologia & Bioengenharia | Scoop.it

Python is an object-oriented programming language that is ideal for biological data analysis. The course will start from zero knowledge, and introduce you to all basic concepts of Python such as calculating, organizing data, reading and writing files, program logic and writing bigger programs. All examples and practical sessions will focus on solving biological problems.

In particular the sessions will cover:
- working with DNA and protein sequences
- data retrieval from files and their manipulation
- running applications, such as BLAST, locally and from a scrip
- finding motifs in sequence
- parsing common file formats (Uniprot, GenBank, PDB, BLAST) with Biopython
- ways to find and correct program errors

The course will be highly interactive and the students will continuously put theory into practice while learning. By the end of the course, the participants will have a good understanding of Python basics and will have acquired the skills to manage any type of bioinformatics database record and to run applications from scripts. Basic Unix/Linux skills will be provided at the beginning of the course.

Léo Felippe Dias's insight:

Ótimo curso para aqueles que almejam utilizar o Python para bioinformática.

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Rescooped by Léo Felippe Dias from Bioinformatics Software: Sequence Analysis
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VarSeq: A bioinformatics Swiss Army knife | Our 2 SNPs…®

VarSeq: A bioinformatics Swiss Army knife | Our 2 SNPs…® | Miscelâneas da Biotecnologia & Bioengenharia | Scoop.it
If you’ve seen the recent webinars given by Gabe Rudy and Bryce Christensen, you’ve no doubt been impressed by the capabilities of VarSeq when it comes to annotation and filtering.

Via Mel Melendrez-Vallard
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Rescooped by Léo Felippe Dias from SynBioFromLeukipposInstitute
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Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells

Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells | Miscelâneas da Biotecnologia & Bioengenharia | Scoop.it

Via Gerd Moe-Behrens
Léo Felippe Dias's insight:

Sintetizando circuitos biológicos para serem utilizados na terapia genética do cancer.

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Gerd Moe-Behrens's curator insight, November 7, 2014 3:31 PM

by
Yuchen Liu, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang & Zhiming Cai

"The conventional strategy for cancer gene therapy offers limited control of specificity and efficacy. A possible way to overcome these limitations is to construct logic circuits. Here we present modular AND gate circuits based on CRISPR-Cas9 system. The circuits integrate cellular information from two promoters as inputs and activate the output gene only when both inputs are active in the tested cell lines. Using the luciferase reporter as the output gene, we show that the circuit specifically detects bladder cancer cells and significantly enhances luciferase expression in comparison to the human telomerase reverse transcriptase-renilla luciferase construct. We also test the modularity of the design by replacing the output with other cellular functional genes including hBAX, p21 and E-cadherin. The circuits effectively inhibit bladder cancer cell growth, induce apoptosis and decrease cell motility by regulating the corresponding gene. This approach provides a synthetic biology platform for targeting and controlling bladder cancer cells in vitro."


http://bit.ly/1xqfP2b