Genetic Engineering - GEG Tech top picks
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T-Cell Immunotherapy Market, 2030 - Focus on CAR-T Therapies, TCR Therapies and TIL Therapies

T-Cell Immunotherapy Market, 2030 - Focus on CAR-T Therapies, TCR Therapies and TIL Therapies | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:

The "T-Cell Immunotherapy Market, 2018-2030 (3rd edition)" report features an extensive study of the current market landscape and the future potential of T-cell immunotherapies (focusing particularly on CAR-T therapies, TCR therapies and TIL therapies). One of the key objectives of the study was to review and quantify the future opportunities associated with the ongoing development programs of both small and big pharmaceutical firms.

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New CRISPR inhibitors found with help from U.S. Department of Defense funding

New CRISPR inhibitors found with help from U.S. Department of Defense funding | Genetic Engineering - GEG Tech top picks | Scoop.it
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Two papers describing anti-CRISPR proteins were supported by a DARPA program to improve the safety of the gene-editing method and to counteract its nefarious use

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Management guidelines for paediatric patients receiving chimeric antigen receptor T cell therapy

Management guidelines for paediatric patients receiving chimeric antigen receptor T cell therapy | Genetic Engineering - GEG Tech top picks | Scoop.it
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Chimeric antigen receptor (CAR) T cell therapies have impressive activity in the treatment of cancer but are associated with potentially fatal toxicities. In light of the approval of CAR T cell therapy for paediatric patients, a panel of experts from the Hematopoietic Stem Cell Transplantation (HSCT) Subgroup of the Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network, the CAR T Cell Therapy-Associated Toxicity (CARTOX) Program at The University of Texas MD Anderson Cancer Center, and several other institutions have developed consensus guidelines for the use and management of these treatments in paediatric patients, which are presented herein.

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Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements | Genetic Engineering - GEG Tech top picks | Scoop.it
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Cas9-induced double stranded breaks can cause large deletions near the target site and more complex genomic rearrangements in mouse and human stem cells.

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A serious new hurdle for CRISPR: Edited cells might cause cancer, find two studies

A serious new hurdle for CRISPR: Edited cells might cause cancer, find two studies | Genetic Engineering - GEG Tech top picks | Scoop.it
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The two studies published Monday are a potential game-changer for companies pursuing CRISPR-based therapies, but as one researcher said, "I don't think it's a deal-breaker."

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CRISPR/Cas9 system targeting regulatory genes of HIV-1 inhibits viral replication in infected T-cell cultures - Science

CRISPR/Cas9 system targeting regulatory genes of HIV-1 inhibits viral replication in infected T-cell cultures - Science | Genetic Engineering - GEG Tech top picks | Scoop.it
Article
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Scientists in Japan have used CRISPR-Cas9 technology to stop human immunodeficiency virus type 1 (HIV-1) replication in latently infected T cells that can’t be controlled using existing drug treatments. The gene-editing approach effectively disrupts two regulatory HIV-1 genes, tat and rev, which are essential for viral replication. Describing their in vitro studies in Scientific Reports, the researchers at Kobe University Graduate School of Medicine and Kobe University Graduate School of Health Sciences say initial results indicate that using CRISPR-Cas9 to target HIV-1 regulatory genes may offer a new approach to achieving “functional cures.”

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Early Success Gives Hope for CAR T-Cell Therapy in Multiple Myeloma

Early Success Gives Hope for CAR T-Cell Therapy in Multiple Myeloma | Genetic Engineering - GEG Tech top picks | Scoop.it
Early trial results show that immunotherapy using chimeric antigen receptor (CAR) T-cell therapy may be effective in patients with multiple myeloma.
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Last year results from two early clinical trials indicated that immunotherapy using chimeric antigen receptor (CAR) T-cell therapy may be effective in patients with multiple myeloma

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The First "Cell-Free" CRISPR Tech Is Here To Personalize Cancer Treatment

The First "Cell-Free" CRISPR Tech Is Here To Personalize Cancer Treatment | Genetic Engineering - GEG Tech top picks | Scoop.it

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CRISPR-Directed In Vitro Gene Editing of Plasmid DNA Catalyzed by Cpf1 (Cas12a) Nuclease and a Mammalian Cell-Free Extract

it the content

BigField GEG Tech's insight:

Researchers have discovered that CRISPR-Cpf1 allows them to make edits to genes outside of the cell, something CRISPR-Cas9 can't do effectively.

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Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars

Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:

A key goal of developmental biology is to understand how a single cell is transformed into a full-grown organism comprising many different cell types. Here th scientists present LINNAEUS (lineage tracing by nuclease-activated editing of ubiquitous sequences)—a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. 

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Base editing with a Cpf1–cytidine deaminase fusion

Base editing with a Cpf1–cytidine deaminase fusion | Genetic Engineering - GEG Tech top picks | Scoop.it
Brief Communication
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The targeting range of CRISPR–Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, the scientists developed a CRISPR–Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing.
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New Clues to CAR T-Cell Failure 

New Clues to CAR T-Cell Failure  | Genetic Engineering - GEG Tech top picks | Scoop.it
Chemo effects, T cell energy sources
BigField GEG Tech's insight:
Recent findings emphasized that the success of CAR T-cell therapy in an individual patient depended on the quality of the starting material, David M. Barrett, MD, PhD, of Children’s Hospital of Pennsylvania in Philadelphia, said during a press briefing prior to the American Association for Cancer Research (AACR) meeting.
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IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival in the tumor

IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival in the tumor | Genetic Engineering - GEG Tech top picks | Scoop.it
Letter
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In this study, the authors engineered CAR-T cells to express interleukin (IL)-7 and CCL19 (7 × 19 CAR-T cells), as these factors are essential for the maintenance of T-cell zones in lymphoid organs.
Following treatment of mice with 7 × 19 CAR-T cells, both recipient conventional T cells and administered CAR-T cells generated memory responses against tumors.
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Cancer ‘vaccine’ eliminates tumors in mice

Cancer ‘vaccine’ eliminates tumors in mice | Genetic Engineering - GEG Tech top picks | Scoop.it
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Activating T cells in tumors eliminated even distant metastases in mice, Stanford researchers found. Lymphoma patients are being recruited to test the technique in a clinical trial.
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CRISPR screen identifies gene that helps cells resist West Nile, Zika viruses - Newsroom, UT Southwestern

CRISPR screen identifies gene that helps cells resist West Nile, Zika viruses - Newsroom, UT Southwestern | Genetic Engineering - GEG Tech top picks | Scoop.it
UT Southwestern researchers today report the first use of CRISPR genome-wide screening to identify a gene that helps cells resist West Nile virus, dengue fever, Zika virus, and yellow fever.
BigField GEG Tech's insight:

In a study published in Nature Microbiology, the team led by Dr. John Schoggins, Assistant Professor of Microbiology, used the cutting-edge CRISPR technology to identify the IFI6 gene as a potent antiviral gene targeting flaviviruses. The researchers then used traditional cell culture studies to confirm the gene’s role in protecting against infection by Zika, West Nile, dengue, and yellow fever viruses.

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CRISPR Gene Editing Treatment for Duchenne Muscular Dystrophy Moves Closer to Clinical Trials

CRISPR Gene Editing Treatment for Duchenne Muscular Dystrophy Moves Closer to Clinical Trials | Genetic Engineering - GEG Tech top picks | Scoop.it
The latest approach to developing a gene therapy for Duchenne muscular dystrophy shows promising results.
BigField GEG Tech's insight:

In the ongoing effort to develop new treatments for Duchenne muscular dystrophy (DMD), investigators say they have moved one step closer toward developing a new gene therapy for patients with the disease.

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Scientists find people's assumptions about DNA repair after CRISPR editing to be wrong

Scientists find people's assumptions about DNA repair after CRISPR editing to be wrong | Genetic Engineering - GEG Tech top picks | Scoop.it
CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
BigField GEG Tech's insight:

Despite high hopes and high investment in CRISPR-Cas9 gene editing, scientists still have a lot to learn about how it works in humans.

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Cancer immunotherapy 'could be safer' with natural killer cells

Cancer immunotherapy 'could be safer' with natural killer cells | Genetic Engineering - GEG Tech top picks | Scoop.it
Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity
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In a paper now published in the journal Cell Stem Cell, the authors describe how the cells showed enhanced "anti-tumor activity" in mice with ovarian cancer seeded from human cancer cells.

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Hybrid human–chicken embryos illuminate key developmental milestone - Nature

Hybrid human–chicken embryos illuminate key developmental milestone - Nature | Genetic Engineering - GEG Tech top picks | Scoop.it
A new technique reveals the earliest stages of human development without the need for human embryos.
BigField GEG Tech's insight:

For the first time, researchers have watched human ‘organizer’ cells direct the formation of an embryo’s top, bottom, front and back. They did so by developing a technique that sidesteps restrictions on research with human embryos by grafting human cells onto chicken embryos. The method, published on 23 May in Nature1, could supplant the use of human embryos in some laboratory experiments.

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Humanized CAR T-cell Therapy Seen to Treat Metastatic Colorectal Cancer in Mouse Study

Humanized CAR T-cell Therapy Seen to Treat Metastatic Colorectal Cancer in Mouse Study | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:
A humanized chimeric antigen receptor (CAR) T-cell immunotherapy was seen successfully kill tumor cells and prevent further spread of colorectal metastatic cancer in mouse models of the disease.
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Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Responses

Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T  Responses | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:

A chimeric antigen receptor system that can integrate signals from multiple antigens
and fine-tune T cell activation in a cell-type-specific manner holds promise for enhancing
the safety and specificity of CAR T cell therapies for cancer treatment.

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Highly parallel genome variant engineering with CRISPR–Cas9

Highly parallel genome variant engineering with CRISPR–Cas9 | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:

Here, the scientists developed a CRISPR-library-based approach for highly efficient and precise genome-wide variant engineering. They used our method to examine the functional consequences of premature-termination codons (PTCs) at different locations within all annotated essential genes in yeast. This approach can be used to profile the effects of large classes of variants in a high-throughput manner.

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Curing the world with CRISPR: Where we are at and where we 're headed

Curing the world with CRISPR: Where we are at and where we 're headed | Genetic Engineering - GEG Tech top picks | Scoop.it
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written by Thomas Clements CRISPR has been hailed as one of the most promising gene editing technologies and promises to revolutionize precision medicine
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Stem Cells Treat Macular Degeneration

Stem Cells Treat Macular Degeneration | Genetic Engineering - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:
Researchers helped develop a specially engineered retinal patch to treat people with sudden, severe sight loss.
The results of this groundbreaking clinical study, published in Nature Biotechnology, describe the effective implantation of a specially engineered patch of retinal pigment epithelium cells derived from stem cells to treat people with sudden severe sight loss from wet AMD -- the form that afflicted Waters. This is the first description of a completely engineered tissue that has been successfully used in this way.
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CRISPR Screen Identifies Genetic Modifiers of ALS

CRISPR Screen Identifies Genetic Modifiers of ALS | Genetic Engineering - GEG Tech top picks | Scoop.it
Study provides roadmap for using CRISPR to investigate neurological disorders.
BigField GEG Tech's insight:
Researchers at Stanford University just published their recent findings in Nature Genetics in an article entitled “CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity.” Using the new gene-editing screen, the researchers uncovered a new set of genes that may hasten neuron death during disease.
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Genome-wide tracking of dCas9-methyltransferase footprints

Genome-wide tracking of dCas9-methyltransferase footprints | Genetic Engineering - GEG Tech top picks | Scoop.it
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Here, the scientists report the generation and use of an engineered, methylation depleted but maintenance competent mouse ES cell line and find surprisingly ubiquitous nuclear activity of dCas9-methyltransferases. Subsequent experiments in human somatic cells refine these observations and point to an important difference between genetic and epigenetic editing tools that require unique experimental considerations.
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