from Flow Cytometry to Cytomics
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Scooped by Gilbert C FAURE
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Counting Cells with a Hemocytometer

There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted?

 

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It is how techniques are taught or learned now! Thanks Youtube

Obviously, this first Scoop is not significant of the information you will find through the following 122 pages of this topic, with many recent informations of developments of flow cytometry, image cytometry... focusing particularly on the detection, quantification, characterization of rare events (CTCs, CECS..) in peripheral blood and biological fluids.

 

more than 3000 scoops, >5200 visitors, 10700 views (march 2015)

 

For data from the author, see the Nancytomique topic.

http://www.scoop.it/t/nancytomique

 

 

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Technical challenges of working with extracellular vesicles - Nanoscale (RSC Publishing)

Technical challenges of working with extracellular vesicles - Nanoscale (RSC Publishing) | from Flow Cytometry to Cytomics | Scoop.it
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content,
SBQ-RSC: Celebrating UK-Brazil collaborations
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Rescooped by Gilbert C FAURE from Cancer Immunotherapy Review
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Flow Cytometry - Driving CAR T Cell Therapy from Bench to Bedside

Flow Cytometry - Driving CAR T Cell Therapy from Bench to Bedside | from Flow Cytometry to Cytomics | Scoop.it
CAR T cell therapy is a custom-made biologic that is time and labor intensive, but an extremely effective form on cancer treatment.

Via Krishan Maggon
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Single-cell analysis identifies a CD33 + subset of human cord blood cells with high regenerative potential

Single-cell analysis identifies a CD33 + subset of human cord blood cells with high regenerative potential | from Flow Cytometry to Cytomics | Scoop.it
Knapp et al. analyse the heterogeneous molecular profiles and functions of CD49f human cord blood haematopoietic stem cells and report that a subset with CD33 expression has improved regenerative activity.
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Imaging mass cytometer (IMC) - Hyperion —

Imaging mass cytometer (IMC) - Hyperion — | from Flow Cytometry to Cytomics | Scoop.it
ACF is an advanced flow cytometry and imaging core facility located in
Sydney, Australia.
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Rescooped by Gilbert C FAURE from PARP Inhibitors Cancer Review
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Identification of the tumour transition states occurring during EMT

Identification of the tumour transition states occurring during EMT | from Flow Cytometry to Cytomics | Scoop.it

In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here we screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states. Although all EMT subpopulations presented similar tumour-propagating cell capacity, they displayed differences in cellular plasticity, invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes identify the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulate EMT transition states.


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Rescooped by Gilbert C FAURE from PARP Inhibitors Cancer Review
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Oncotarget | Circulating tumor cells, tumor-derived extracellular vesicles and plasma cytokeratins in castration-resistant prostate cancer patients

Oncotarget | Circulating tumor cells, tumor-derived extracellular vesicles and plasma cytokeratins in castration-resistant prostate cancer patients | from Flow Cytometry to Cytomics | Scoop.it
Circulating tumor cells, tumor-derived extracellular vesicles and plasma cytokeratins in castration-resistant prostate cancer patients...

Via Krishan Maggon
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Multivariate computational analysis of biosensor's data for improved CD64 quantification for sepsis diagnosis - Lab on a Chip (RSC Publishing)

Multivariate computational analysis of biosensor's data for improved CD64 quantification for sepsis diagnosis - Lab on a Chip (RSC Publishing) | from Flow Cytometry to Cytomics | Scoop.it
Sepsis, as a leading cause of death worldwide, relies on systemic inflammatory response syndrome (SIRS) criteria for its diagnosis. SIRS is highly non-specific as it relies on monitoring of patients' vitals for sepsis diagnosis, which are known to change with many confounding factors.
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Fluidigm | Consumables | Maxpar® Human Immune Monitoring Panel Kit

Fluidigm | Consumables | Maxpar® Human Immune Monitoring Panel Kit | from Flow Cytometry to Cytomics | Scoop.it
Maxpar® Human Immune Monitoring Panel Kit, 29 Marker-25 Tests: This panel kit contains 29 antibodies designed and optimized for deep immune profiling of human PBMC by comprehensive identification and characterization of key immune cell populations.
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Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry. - PubMed - NCBI

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry. - PubMed - NCBI | from Flow Cytometry to Cytomics | Scoop.it
J Vis Exp. 2017 Apr 19;(122). doi: 10.3791/55283.Video-Audio Media...
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Early stage NSCLC — challenges to implementing ctDNA-based screening and MRD detection

Early stage NSCLC — challenges to implementing ctDNA-based screening and MRD detection | from Flow Cytometry to Cytomics | Scoop.it
Liquid biopsy approaches hold great promise in early cancer diagnosis or minimal residual disease monitoring for cancer recurrence. Herein, the authors evaluate contemporary next-generation sequencing approaches to circulating tumour DNA detection in these contexts, with a focus on studies in patients with non-small-cell lung cancer. They discuss the feasibility of introducing these strategies into the clinic, highlighting the technical and analytical challenges, as well as possible solutions.
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Study: Blood Test for Prostate Cancer Can Guide Therapy, Extend Lives

Study: Blood Test for Prostate Cancer Can Guide Therapy, Extend Lives | from Flow Cytometry to Cytomics | Scoop.it
Blood tests for cancer, known as liquid biopsies, have become available in recent years to guide treatments for people already diagnosed. But how useful
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Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood | Projects | FP7

Cancer treatment and monitoring through identification of circulating tumour cells and tumour related nucleic acids in blood | Projects | FP7 | from Flow Cytometry to Cytomics | Scoop.it
FP7,CANCER-ID,Blood-based biomarkers such as Circulating Tumor Cells (CTCs), circulating free tumor DNA (cfDNA) and microRNAs (miRNAs) have the potential to improve the development of personalized medicines for cancer patients. This is of particular importance when biopsies of the primary ...,IMI-JU-11-2013-03,TATAA BIOCENTER AB(SE),HEINRICH-HEINE-UNIVERSITAET DUESSELDORF(DE),Institut Gustave Roussy(FR),HELSINGIN JA UUDENMAAN SAIRAANHOITOPIIRIN KUNTAYHTYMÄ(FI),THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE(UK),Siemens Healthcare Diagnostics Products GMBH(DE),LUXEMBOURG INSTITUTE OF HEALTH(LU),THE UNIVERSITY OF MANCHESTER(UK),MEDIZINISCHE UNIVERSITAT GRAZ(AT),ETHNIKO KAI KAPODISTRIAKO PANEPISTIMIO ATHINON(EL),European Organisation for Research and Treatment of Cancer(BE),ACADEMISCH ZIEKENHUIS GRONINGEN(NL),UNIVERSITEIT TWENTE(NL),Clearbridge Biomedics Pte Ltd(SG),MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV(DE),OSLO UNIVERSITETSSYKEHUS HF(NO),DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG(DE),UNIVERSITE DE MONTPELLIER(FR),UNIVERSITA DEGLI STUDI DI TORINO(IT),LEUKOCARE AG(DE),MENARINI SILICON BIOSYSTEMS SPA(IT),ORION OYJ(FI),ALACRIS THERANOSTICS GMBH(DE),QIAGEN GMBH(DE),INSTITUT CURIE(FR),GABO:MI GESELLSCHAFT FUR ABLAUFORGANISATION:MILLIARIUM MBH & CO KG(DE),Boehriger Ingelheim International GmbH(DE),CHARITE - UNIVERSITAETSMEDIZIN BERLIN(DE),ARTTIC(FR),UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF(DE),TERUMO BCT EUROPE NV(BE),Bayer Aktiengesellschaft(DE),Participant Legal name Istituto Oncologico Veneto IRCCS(IT),ANGLE EUROPE LIMITED(UK),Institut de Recherches Servier(FR),VyCAP BV(NL),Agilent Technologies Sales & Services GmbH & Co. KG(DE),Eli Lilly and Company Ltd(UK),GILUPI GmbH(DE)
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2015-2019

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The need for sensitivity - The CytoFlex family and new capabilities

The need for sensitivity - The CytoFlex family and new capabilities | from Flow Cytometry to Cytomics | Scoop.it
Our Imaging and Cytometry Core Lab is in the Bioscience Technology Facility that serves a multidisciplinary Department of Biology as well as external users through our open access approach.&n
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A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites

A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites | from Flow Cytometry to Cytomics | Scoop.it
Author summary African sleeping sickness is caused by Trypanosoma brucei. Tens of millions of people living in endemic areas are at risk for the disease. Within the mammalian bloodstream, T. brucei parasites sustain all their energy needs by metabolizing glucose present in the host’s blood within...
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Single-cell approaches to immune profiling

Single-cell approaches to immune profiling | from Flow Cytometry to Cytomics | Scoop.it
Protein- and sequencing-based technologies are helping researchers to profile immune cells ever more deeply.
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Home - ESCCA - European society for clinical cell analysis

Home - ESCCA - European society for clinical cell analysis | from Flow Cytometry to Cytomics | Scoop.it
ESCCA, European society for clinical cell analysis
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valencia 2018

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Mass cytometry: a powerful tool for dissecting the immune landscape - ScienceDirect

Mass cytometry: a powerful tool for dissecting the immune landscape - ScienceDirect | from Flow Cytometry to Cytomics | Scoop.it
Advancement in methodologies for single cell analysis has historically been a major driver of progress in immunology. Currently, high dimensional flow…
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Biocompare Launches Trends in Flow Cytometry Future Lab | news.sys-con.com

Biocompare Launches Trends in Flow Cytometry Future Lab | news.sys-con.com | from Flow Cytometry to Cytomics | Scoop.it
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Flow cytometry for assessing the microbiology of drinking water - from source to tap

Flow cytometry for assessing the microbiology of drinking water - from source to tap | from Flow Cytometry to Cytomics | Scoop.it
This website has been designed for modern browsers. To experience its full functionality please enable JavaScript. Without JavaScript you may encounter issues using the site. [Never show this message] Close Analytical Biological Environmental General Interest Human Health Industry Flow cytometry for assessing the microbiology of drinking water - from source to tap 15 May 2018 09:30-16:30, London, United Kingdom Introduction Objectives For those who have not used the technique, this seminar will provide background, theory and applications of flow cytometry to the analysis of drinking water. For those familiar with the technique, the seminar and associated exhibition provide an opportunity to find out about the latest developments in laboratory based and on-line instrumentation, to hear from leading researchers in the area, and for knowledge exchange with other practitioners.   Who should attend • Water industry personnel – analytical and operational scientists, water quality staff, laboratory managers. • Suppliers and manufacturers of flow cytometry instrumentation   • Researchers concerned with the application of flow cytometry to water supplies • Water engineers and consultants working for the water industry • Regulators responsible for legislation relating to drinking water quality Contents Presentations will be given by leading field experts, regulatory bodies and water companies. The full agenda details can be found on the flier link. Downloads Venue Organised by Contact information Related events BNASS 2018: The 19th Biennial National Atomic Spectroscopy Symposium 2 - 4 July 2018, London, United Kingdom Artificial Water Channels: Faraday Discussion 25 - 27 June 2018, Glasgow, United Kingdom Enhanced Performance using Additives; Choosing the Best Additive 20 June 2018 10:00 - 21 June 2018 15:00, Cologne, Germany The geochemistry and mineralogy of contaminated environments 6 June 2018 09:30-17:00, London, United Kingdom SEARCH  EXCLUDE ONLINE EVENTS PLEASE CHECK TO FILTER ON DATE RANGE  SELECT DATE RANGE Showing all upcoming events START DATE END DATE LOCATION All locations Continents UK & Ireland SUBJECT AREA EVENT TYPE All event types ChemNet Competition Conference / Symposium CPD & Careers Demonstration Exhibitions Lecture Meeting RSC Prizes & Awards Schools Event Social / Networking Tour Training Webinar (Online only) Workshop Explore Membership © Royal Society of Chemistry 2018 Registered charity number: 207890
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Education Links & E-Learning | Bangs Laboratories, Inc.

Education Links & E-Learning | Bangs Laboratories, Inc. | from Flow Cytometry to Cytomics | Scoop.it
Flow CytometryBD Biosciences Training & support page for BD Biosciences, a medical technology company that manufactures and sells a broad range of medical supplies, devices, laboratory equipment and diagnostic products.
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Mass cytometry: a powerful tool for dissecting the immune landscape

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Of Mice and Metastasis | The Scientist Magazine®

Of Mice and Metastasis | The Scientist Magazine® | from Flow Cytometry to Cytomics | Scoop.it
Tools for studying how cancer spreads
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