Fast and accurate identification - microbiology with MALDI-TOF biotyper
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Fast and accurate identification - microbiology with MALDI-TOF biotyper
microbiology with MALDI-TOF biotyper: bacteria, fungi, yeast etc.
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early detection of β-lactamase-producing Enterobacteriaceae by MALDI-TOF analysis #microbiology: 100% sens & spec

early detection of β-lactamase-producing Enterobacteriaceae by MALDI-TOF analysis #microbiology: 100% sens & spec | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Objectives: Development of a novel MALDI-TOF MS-based method for the rapid detection of ESBL-producing Enterobacteriaceae.Methods: The method was evaluated in terms of sensitivity, specificity and turnaround time regarding the antibiotic used (cefotaxime, ceftazidime, ceftriaxone, cefpodoxime or cefepime) and the performance of the automated MBT STAR-BL software (Bruker Daltonik GmbH, Germany) relative to qualitative interpretation of spectra for detecting β-lactamase resistance by MALDI-TOF MS (Bruker Daltonik) in a collection of 11 isogenic Escherichia coli control strains expressing different β-lactamases. Finally, for clinical validation, β-lactamase activity was determined under previously evaluated conditions in 100 clinical isolates previously characterized by PCR and sequencing.Results: Clinical validation of the assay showed 100% sensitivity and specificity for detecting β-lactam resistance in 30 min by measuring hydrolysis of ceftriaxone (0.50 mg/mL) with the automated MBT STAR-BL software. Regarding the antibiotics evaluated, ceftriaxone yielded 70% more positive results than cefotaxime, 80% more than ceftazidime and 20% more than cefpodoxime, with 100% specificity. Cefepime revealed 100% sensitivity, but only 27% specificity. For the same incubation time, the automated software yielded on average 41% more positive results in relation to detecting resistance than qualitative interpretation of spectra.Conclusions: Our clinical validation of the method proved it to be highly reliable, simple to perform and time saving, transforming β-lactam resistance detection by MALDI-TOF MS into a ready-to-use technique in clinical laboratories.
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Quote: "Clinical validation of the assay showed 100% sensitivity and specificity for detecting β-lactam resistance in 30 min"!!!
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MALDI-ToF short incubation ID blood cultures: reduced hospitalization & decreased bacteremia mortality

MALDI-ToF short incubation ID blood cultures: reduced hospitalization & decreased bacteremia mortality | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Eur J Clin Microbiol Infect Dis. 2017 Jul;36(7):1181-1186. doi: 10.1007/s10096-017-2906-y. Epub 2017 Jan 20.
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Microbiology #ASM2017 New Orleans keynote lecture june 3, 6.15 PM: dr. Kate Rubins, NASA astronaut scientist!!

Microbiology #ASM2017 New Orleans keynote lecture june 3, 6.15 PM: dr. Kate Rubins, NASA astronaut scientist!! | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
The American Society for Microbiology (ASM) is the oldest and largest single life science membership organization in the world. Membership has grown from 59 scientists in 1899 to more than 39,000 members today, with more than one third located outside the United States. The members represent 26 disciplines of microbiological specialization plus a division for microbiology educators.
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ScienceDirect: Identification of microorganisms grown on chromogenic media by MALDI-TOF MS

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Highlights • Chromogenic media do not reduce the diagnostic quality of MALDI-TOF MS. • Chromogenic media does not result in false identifications of microbial pathogens. • Chromogenic media can be used to support microbial identification by MALDI-TOF MS. Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS. Among the tested isolates, no misidentification was recorded and there was no medium-related difference in the identification level. However, score values were overall slightly but significantly lower for isolates grown on chromogenic media. In conclusion, the use of chromogenic culture media tested here had no relevant impact on MALDI-TOF MS performance for diagnostic purposes.
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Fast & accurate ID in microbiology- maldi biotyper system: Topics by Science.gov peer reviewed abstracts

Fast & accurate ID in microbiology- maldi biotyper system: Topics by Science.gov peer reviewed abstracts | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
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Viriginia Commonwealth OCT 2016: Validation, Verification, and Implementation of MALDI-TOF MS in medical microbiology

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New Hampshire veterinary microbiology: Identifying Infections in Animals Now Takes Minutes at the NH Veterinary Diagnostic Lab

New Hampshire veterinary microbiology: Identifying Infections in Animals Now Takes Minutes at the NH Veterinary Diagnostic Lab | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
When the state experiences an outbreak of an infectious disease in animals, the time it takes to identify the disease can have a significant impact on the treatment and outbreak response. Now, that time period has been sharply reduced from what could take days, down to minutes at the NH Veterinary Diagnostic Lab thanks to the acquisition of a sophisticated type of mass spectrometry.
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Mass spectrometry for the discovery of biomarkers of sepsis - Molecular BioSystems (RSC Publishing)

Mass spectrometry for the discovery of biomarkers of sepsis - Molecular BioSystems (RSC Publishing) | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
“ Sepsis is a serious medical condition that occurs in 30% of patients in intensive care units (ICUs). Early detection of sepsis is key to prevent its progression to severe sepsis and septic shock, which can cause organ failure and death. Diagnostic criteria for sepsis are nonspecific and hinder a timely diagnosis in ”
Via sjossie
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Nomination #BRUKER rapifleX MALDI PharmaPulse High Throughput Screening: Best New #Drug Discovery & Development Product of 2016. #Pharma Vote

Nomination #BRUKER rapifleX MALDI PharmaPulse High Throughput Screening: Best New #Drug Discovery & Development Product of 2016. #Pharma Vote | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it

Web survey powered by SurveyMonkey.com. Create your own online survey now with SurveyMonkey's expert certified FREE templates.

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We are very happy that the rapifleX™ MALDI PharmaPulse™ has been nominated for Best New Drug Discovery & Development Product of 2016 in the Select Science Scientists’ Choice Awards®.

Voting closes on Friday January 27, 2017. The winning product will be announced at the annual Scientists’ Choice Awards Party during SLAS2017 in Washington, DC on Tuesday Feb 6, 2017.

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Guardian.com Bill Gates: world faces decade at risk from antibiotic-resistant bugs #AntibioticStewardship

Guardian.com Bill Gates: world faces decade at risk from antibiotic-resistant bugs #AntibioticStewardship | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Philanthropist says he believes ‘better medical tools’ will come, but until then the world is vulnerable to a pandemic
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Pluimvee 63 December 2016: GD Deventer Animal Health explains fast MALDI-TOF to identify avian bacterial infections (in Dutch)

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Megan Murn Microbiologics.com blog : How to Implement MALDI-TOF Technology in Your Lab - #Bruker $BRKR

Megan Murn Microbiologics.com blog : How to Implement MALDI-TOF Technology in Your Lab - #Bruker $BRKR | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
For over 100 years, bacteria have been identified by their biochemical properties through the use of various test methods. With the addition of MALDI-TOF Mass Spectrophotometry (MS) technology, thi…
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MALDI-ToF is poised to speed diagnosis for bacterial and fungal infections - MLO

MALDI-ToF is poised to speed diagnosis for bacterial and fungal infections - MLO | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Helping clinicians quickly and effectively address the medical concerns of their patients should be the ultimate goal of any clinical laboratory. Delivering on this goal can be especially important when a patient presents with a bacterial or fungal infection. These infections, ranging from methicillin resistant Staphylococcus aureus (MRSA) cellulitis to Gram-negative sepsis, can manifest rapidly,
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MALDI BioTyper vs 16S rDNA #sequencing 4 identification #Environmental #bacteria isolated from #Arctic sea #water

MALDI BioTyper vs 16S rDNA #sequencing 4 identification #Environmental #bacteria isolated from #Arctic sea #water | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
PLoS One. 2017 Jul 24;12(7):e0181860. doi: 10.1371/journal.pone.0181860. eCollection 2017.
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Microbiology Subtyping: automated differentiation of Mycobacterium chimaera / intracellular complex / Listeria monocytogenes & relatives

Microbiology Subtyping: automated differentiation of Mycobacterium chimaera / intracellular complex / Listeria monocytogenes & relatives | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
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Bruker: MALDI Biotyper User Meeting Netherlands Jaarbeurs Utrecht - ECCMID intro updates!

Bruker: MALDI Biotyper User Meeting Netherlands Jaarbeurs Utrecht - ECCMID intro updates! | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
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Nature.com: Bruker MALDI-TOF MS as alternative for capsular typing Streptococcus pneumoniae isolates

Nature.com: Bruker MALDI-TOF MS as alternative for capsular typing Streptococcus pneumoniae isolates | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Streptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques.
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Christ et al - Superior MALDI-TOF vs API strip Characterization of Enterococcus species isolated from marine recreational waters

Christ et al - Superior MALDI-TOF vs API strip Characterization of Enterococcus species isolated from marine recreational waters | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Mar Pollut Bull. 2017 Mar 17. pii: S0025-326X(17)30230-8. doi: 10.1016/j.marpolbul.2017.03.025. [Epub ahead of print]
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 Clinical Lab Products #CLP Magazine:  Emerging Applications for MALDI-TOF MS by C. Doern Virginia Commonwealth Microbiology interview

 Clinical Lab Products #CLP Magazine:  Emerging Applications for MALDI-TOF MS by C. Doern Virginia Commonwealth Microbiology interview | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
AMP report maps progress toward identification of filamentous fungi and mycobacteria
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Dr Gary Lum Australian microbiology blog - Medical Fun Facts Episode 47: MALDI-TOF fast and accurate species identification

Dr Gary Lum Australian microbiology blog - Medical Fun Facts Episode 47: MALDI-TOF fast and accurate species identification | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
If you’re not a microbiologist or chemist, I bet you’re thinking I’m about
to launch into a show about fungi. Well, no, I’m still doing the
diagnostics series of Medical Fun Facts.

MALDI-TOF is an acronym for Matrix-Assisted LASER Desorption/Ionisation
Time of Flight. And, just to be a pedant, LASER is an acronym for Light
Amplification by Stimulated Emission of Radiation.
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Molecular & MALDI-TOF Characterization of Clinically Significant Melanized Fungi in India #RADBOUD #Nijmegen

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Finance.yahoo.com summarizes: Bruker Buys InVivo Biotech, Boosts MALDI Biotyper Suite -$BRKR will double 90 m$ franchise in 4 yrs

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The Clinical Impact of Rapid, Direct MALDI-ToF Identification of Bacteria from Positive Blood Cultures

The Clinical Impact of Rapid, Direct MALDI-ToF Identification of Bacteria from Positive Blood Cultures | Fast and accurate identification - microbiology with MALDI-TOF biotyper | Scoop.it
Background Faster identification of bacterial isolates from blood cultures can enable earlier clinical intervention for patients with sepsis. We evaluated the clinical impact of direct identification of micro-organisms from positive blood cultures using MALDI-ToF. Method Positive blood cultures with organisms seen on Gram stain were included over a four week period. For each patient case, comparison was made between the clinical advice given on day one with only a Gram stain result, and the follow up advice given on day two with the benefit of organism identification. Culture results were then compared with direct MALDI-ToF identification. Results For 73 of 115 cases (63.5%), direct organism identification was obtained by MALDI-ToF. Of those 73, 70 (95.5%) had a result concordant with that of the plate culture. In 28 of the 115 cases (24.3%) direct MALDI-ToF identification on day one would have had a clear clinical benefit. In 11 cases it would have helped to identify the potential source of bacteraemia. In 11 cases it would have indicated a different antibiotic regimen on day one, with five patients receiving appropriate antibiotics 24 hours earlier. For 14 cases the blood culture isolate could have been designated as unlikely to be clinically significant. Conclusion We have demonstrated that organism identification on day one of blood culture positivity can have a direct clinical impact. Faster identification using MALDI-ToF assists the clinician in assessing the significance of a blood culture isolate on day one. It can allow earlier appropriate choice of antimicrobial agent, even in the absence of susceptibility testing, and help narrow down the potential source of infection providing a focus for further investigation in a more timely way than conventional techniques alone.
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MALDI-TOF for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria with antibiotic susceptibility test rapid assay MBT-ASTRA

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Zohorul Islam, Copenhagen & India - YouTube: "How to identify bacteria and fungi by MALDI-TOF" >3300 views in 1 week!

Special Thanks to Øystein Angen for his guidelines to learn the technique Matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF) Th
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