CENTROSOME
21.1K views | +0 today
Follow
CENTROSOME
centriole, basal body, centrosome and cilia
Curated by PaG
Your new post is loading...
Your new post is loading...
Scooped by PaG
Scoop.it!

"The centrosome renaissance" compiled and edited by Michel Bornens and Pierre Gönczy

"The centrosome renaissance" compiled and edited by Michel Bornens and Pierre Gönczy | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies | CENTROSOME | Scoop.it
Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the nine-fold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted C entral C oiled- C oil D omain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 Å revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure.
more...
No comment yet.
Scooped by PaG
Scoop.it!

Methods in Cell Biology: Cilia & Flagella

Methods in Cell Biology: Cilia & Flagella | CENTROSOME | Scoop.it
Methods in Cell Biology
Volume 127, Pages 1-542 (2015)

 
Methods in Cilia & Flagella


Edited by Renata Basto and Wallace F. Marshall
more...
No comment yet.
Scooped by PaG
Scoop.it!

La scoliose familiale est suscitée par un gène...centriolaire !

La scoliose familiale est suscitée par un gène...centriolaire ! | CENTROSOME | Scoop.it
UdeMNouvelles
PaG's insight:

"Un variant dans le gène POC5 a d'abord été identifié par séquençage d'ADN (séquençage de l'exome) des échantillons provenant d'une grande famille française dont plusieurs membres étaient atteints de scoliose idiopathique"

more...
No comment yet.
Scooped by PaG
Scoop.it!

Proteomic Analysis of Isolated Ciliary Transition Zones Reveals the Presence of ESCRT Proteins

Proteomic Analysis of Isolated Ciliary Transition Zones Reveals the Presence of ESCRT Proteins | CENTROSOME | Scoop.it
PaG's insight:

- Ciliary transition zones were isolated from Chlamydomonas

- Proteomic analysis of the transition zones identified 115 proteins

- Six ESCRT proteins were identified associated with the transition zone

more...
No comment yet.
Scooped by PaG
Scoop.it!

Plk4 Phosphorylates Ana2 to Trigger Sas6 Recruitment and Procentriole Formation

PaG's insight:

•Plk4 phosphorylates Ana2 at essential residues in its conserved STAN motif

•Plk4 phosphorylation triggers the direct interaction of Ana2 with Sas6

•Ana2 phosphorylated by Plk4 recruits Sas6 to centrioles at the end of mitosis

•A phospho-null Ana2 mutant fails to recruit Sas6 and duplicate centrioles 

 

 

more...
No comment yet.
Scooped by PaG
Scoop.it!

Nature Communications: The deubiquitinating enzyme CYLD controls apical docking of basal bodies in ciliated epithelial cells

Nature Communications: The deubiquitinating enzyme CYLD controls apical docking of basal bodies in ciliated epithelial cells | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

Polo-like Kinase 4 Shapes Up - MS Levine and Holland AJ

Polo-like Kinase 4 Shapes Up - MS Levine and Holland AJ | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

Proteomic analysis of mammalian sperm cells identifies new components of the centrosome

more...
No comment yet.
Scooped by PaG
Scoop.it!

- The centrosome renaissance - compiled and edited by Michel Bornens and Pierre Gönczy

- The centrosome renaissance - compiled and edited by Michel Bornens and Pierre Gönczy | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication

more...
No comment yet.
Scooped by PaG
Scoop.it!

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

Settling an old debate: Researchers solve a lingering mystery of cancer cell biology

Settling an old debate: Researchers solve a lingering mystery of cancer cell biology | CENTROSOME | Scoop.it
German biologist Theodor Boveri observed early in the last century that cancer cells often harbor multiple copies of a subcellular structure that he had previously named the centrosome. He was also the first to suggest that the extra centrosomes drive cancer. Biologists have since learned a great deal about the structure and many functions of Boveri’s “special organ of cell division.” But why cancer cells harbor multiple copies of this organelle—and whether they are “addicted” to having so many—
more...
No comment yet.
Scooped by PaG
Scoop.it!

COMMON VARIANTS SPANNING PLK4 ARE ASSOCIATED WITH MITOTIC-ORIGIN ANEUPLOIDY IN HUMAN EMBRYOS

Aneuploidy, the inheritance of an atypical chromosome complement, is common in early human development and is the primary cause of pregnancy loss. By screening day-3 embryos during in vitro fertilization cycles, we identified an association between aneuploidy of putative mitotic origin and linked genetic variants on chromosome 4 of maternal genomes. This associated region contains a candidate gene, Polo-like kinase 4 (PLK4), that plays a well-characterized role in centriole duplication and has the ability to alter mitotic fidelity upon minor dysregulation. Mothers with the high-risk genotypes contributed fewer embryos for testing at day 5, suggesting that their embryos are less likely to survive to blastocyst formation. The associated region coincides with a signature of a selective sweep in ancient humans, suggesting that the causal variant was either the target of selection or hitchhiked to substantial frequency.

more...
No comment yet.
Scooped by PaG
Scoop.it!

Global genetic analysis in mice unveils central role for cilia in congenital heart disease - Nature

more...
No comment yet.
Scooped by PaG
Scoop.it!

Centriole amplification by mother and daughter centrioles differs in multiciliated cells - Nature - Jord et al.

Centriole amplification by mother and daughter centrioles differs in multiciliated cells - Nature - Jord et al. | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

An essential role of the basal body protein SAS-6 in Plasmodium male gamete development and malaria transmission

more...
No comment yet.
Scooped by PaG
Scoop.it!

Stabilization of Cartwheel-less Centrioles for Duplication Requires CEP295-Mediated Centriole-to-Centrosome Conversion

Stabilization of Cartwheel-less Centrioles for Duplication Requires CEP295-Mediated Centriole-to-Centrosome Conversion | CENTROSOME | Scoop.it
more...
No comment yet.
Scooped by PaG
Scoop.it!

Functional Characterization of CFI-400945, a Polo-like Kinase 4 Inhibitor, as a Potential Anticancer Agent

PaG's insight:

- CFI-400945 is a PLK4 small molecule inhibitor with significant anticancer activity

- CFI-400945 causes dysregulated centriole duplication, mitotic errors, and cell death

- CFI-400945 may represent a therapeutic option for a range of solid tumors

more...
No comment yet.