Cell - Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression | Cas9 | Scoop.it



-->Inactive CRISPR associated 9 protein (dCas9) is repurposed for genome engineering


-->dCas9 and a complementary short guide RNA can target specific genomic sites


-->CRISPR interference (CRISPRi) can regulate multiple genes without off-target effects


-->CRISPRi is compact and can be ported to bacterial and mammalian cells