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CHO Cells Can Make More Protein. - PubMed - NCBI

CHO Cells Can Make More Protein. - PubMed - NCBI | Biopharm | Scoop.it
Cell Syst. 2016 Nov 23;3(5):412-413. doi: 10.1016/j.cels.2016.11.007.
H. Fai Poon's insight:
Need paper
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Differential Biological Activities of Swine Interferon-α Subtypes. - PubMed - NCBI

Differential Biological Activities of Swine Interferon-α Subtypes. - PubMed - NCBI | Biopharm | Scoop.it
J Interferon Cytokine Res. 2015 Dec;35(12):990-1002. doi: 10.1089/jir.2015.0076. Epub 2015 Oct 8.
H. Fai Poon's insight:
interferon for pigs. thats a possible product we can develop.
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Isolation of Biosimilar isoforms and product related impurities using N-Rich

Isolation of Biosimilar isoforms and product related impurities using N-Rich | Biopharm | Scoop.it
H. Fai Poon's insight:
This is useful for the analytical folks!
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High-level expression and preparation of recombinant human fibrinogen as biopharmaceuticals. - PubMed - NCBI

High-level expression and preparation of recombinant human fibrinogen as biopharmaceuticals. - PubMed - NCBI | Biopharm | Scoop.it
J Biochem. 2016 Feb;159(2):261-70. doi: 10.1093/jb/mvv099. Epub 2015 Oct 15.
H. Fai Poon's insight:
Not really high level! but it is cool to have 3 subnuit.
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The top 20 drugs in 2020--worldwide sales | FiercePharma

Forecasting drug sales is a tricky business; just ask any biopharma CEO who has overpromised and underdelivered. The people who make it their business know that very well, and they update their numbers with every big shift in the market and every surprise result from a key clinical trial.
H. Fai Poon's insight:
I think I notice is that Mab is not as dominate as now. Small molecule is making a come back!
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Generation of stable CHO pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system. - PubMed - NCBI

Generation of stable CHO pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system. - PubMed - NCBI | Biopharm | Scoop.it
Biotechnol Prog. 2016 Jun 2. doi: 10.1002/btpr.2307. [Epub ahead of print]
H. Fai Poon's insight:
7.6g pool
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COMBINED METABOLOMICS AND PROTEOMICS REVEALS HYPOXIA AS A CAUSE OF LOWER PRODUCTIVITY ON SCALE-UP TO A 5000-LITER CHO BIOPROCESS. - PubMed - NCBI

COMBINED METABOLOMICS AND PROTEOMICS REVEALS HYPOXIA AS A CAUSE OF LOWER PRODUCTIVITY ON SCALE-UP TO A 5000-LITER CHO BIOPROCESS. - PubMed - NCBI | Biopharm | Scoop.it
Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600030. [Epub ahead of print]
H. Fai Poon's insight:
this is a good paper. i think antioxidant can help scale up
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First HIV mAb ibalizumab hits PhIII target | FierceBiotech

First HIV mAb ibalizumab hits PhIII target | FierceBiotech | Biopharm | Scoop.it
Canadian specialty pharma Theratechnologies and its biotech partner TaiMed Biologics have posted positive top-line results for the first-ever antibody HIV treatment.
H. Fai Poon's insight:
This is a good drug that will benefit many patients.
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Components of yeast (Sacchromyces cervisiae) extract as defined media additives that support the growth and productivity of CHO cells. - PubMed - NCBI

Components of yeast (Sacchromyces cervisiae) extract as defined media additives that support the growth and productivity of CHO cells. - PubMed - NCBI | Biopharm | Scoop.it
J Biotechnol. 2016 May 7. pii: S0168-1656(16)30218-8. doi: 10.1016/j.jbiotec.2016.04.031. [Epub ahead of print]
H. Fai Poon's insight:
Very good way to develop a CD media
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Doctors Baffled By Two Brothers Who Become Paralyzed in Vegetative State After The Sun Sets

Doctors Baffled By Two Brothers Who Become Paralyzed in Vegetative State After The Sun Sets | Biopharm | Scoop.it
Two brothers who became paralyzed at night are baffling doctors trying to help.
H. Fai Poon's insight:
very interesting condition
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China's cabinet bans wholesalers from vaccine supply chain | FiercePharma

China's cabinet bans wholesalers from vaccine supply chain | FiercePharma | Biopharm | Scoop.it
China has officially banned wholesalers from acting as brokers in the sales of voluntary vaccines following a scandal that saw nearly $90 million in illegal sales over a 5-year period.
H. Fai Poon's insight:
china is working to make their health care program presentable to developed countries
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The top 10 (possible) blockbusters that (might) launch this year | FierceBiotech

The top 10 (possible) blockbusters that (might) launch this year | FierceBiotech | Biopharm | Scoop.it
Can't get enough of sell-side estimates on the big new drugs in the late-stage pipeline? Then we have a treat for you today.
H. Fai Poon's insight:
a lot of mab. a lot of potential.
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GSK doubles down on Zymeworks’ platform technologies with $908M deal | FierceBiotech

GSK doubles down on Zymeworks’ platform technologies with $908M deal | FierceBiotech | Biopharm | Scoop.it
The pharma giant has stepped back up to the bargaining table to nail down access to a second platform technology that will be employed to develop new, bispecific antibodies.
H. Fai Poon's insight:
bispecific and cart t meodifier. this technology have a lot of potential. i hope theybdont shelf it like merch shelf glyco fi
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Arginine as an eluent overcomes the hindrance of monoclonal antibody quantification by dextran sulfate in protein A affinity chromatography. - PubMed - NCBI

Arginine as an eluent overcomes the hindrance of monoclonal antibody quantification by dextran sulfate in protein A affinity chromatography. - PubMed - NCBI | Biopharm | Scoop.it
Biotechnol Prog. 2015 Nov-Dec;31(6):1536-41. doi: 10.1002/btpr.2164. Epub 2015 Sep 12.
H. Fai Poon's insight:
For
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Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures. - PubMed - NCBI

Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures. - PubMed - NCBI | Biopharm | Scoop.it
J Biosci Bioeng. 2016 Mar;121(3):317-24. doi: 10.1016/j.jbiosc.2015.07.007. Epub 2015 Oct 9.
H. Fai Poon's insight:
can they convince the world to convert from CHO?
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Stabilization and immune response of HBsAg encapsulated within poly(lactic-co-glycolic acid) microspheres using HSA as a stabilizer. - PubMed - NCBI

Stabilization and immune response of HBsAg encapsulated within poly(lactic-co-glycolic acid) microspheres using HSA as a stabilizer. - PubMed - NCBI | Biopharm | Scoop.it
Int J Pharm. 2015 Dec 30;496(2):332-41. doi: 10.1016/j.ijpharm.2015.10.004. Epub 2015 Oct 21. Research Support, Non-U.S. Gov't
H. Fai Poon's insight:
Encapsulation technology. Can it be use in Cells?
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Risk of developing active tuberculosis in rheumatoid arthritis patients on adalimumab in Japan. - PubMed - NCBI

Risk of developing active tuberculosis in rheumatoid arthritis patients on adalimumab in Japan. - PubMed - NCBI | Biopharm | Scoop.it
Int J Tuberc Lung Dis. 2016 Jan;20(1):101-8. doi: 10.5588/ijtld.15.0283. Research Support, Non-U.S. Gov't
H. Fai Poon's insight:
Adalimumab increase TB risk!
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Peregrine Nixes Planned Trials for Bavituximab But Hints at a Future Trial with AstraZeneca PLCs Durvalumab

Peregrine Nixes Planned Trials for Bavituximab But Hints at a Future Trial with AstraZeneca PLCs Durvalumab | Biopharm | Scoop.it
H. Fai Poon's insight:
immunology targets are heating up.
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【5篇Cell、Nature、Science】冷冻电镜分辨率突破2Å,颜宁等科学家连发新成果!

H. Fai Poon's insight:
Cryo EM application will greatly accelerate drug discovery am design. it might bring back small molecule.
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Several Questions about Cell Culture Media from a Chinese PHD Students 中国研究生对细胞培养基的一些问题:

1) 这是我用DOE混合模块做的实验,见附件,但是细胞在此预测的最优表达量的混合培养基中长的不好,维持时间特别短!不知道为什么? 答:因为细胞长得好了,所以把培养基其中的营养加速用光,所以就死得快。 2)老师我想咨询下,关于优化后的个性化无血清培养基,氨基酸和维生素的含量是DMEM中含量的几倍(一般情况)? 答:都不一样,可以5-10倍 3)老师上次您讲座中提到的低G0或者是高G0的平台化无血清培养基,是不是加入了乙酸钾或者丁酸钠等物质抑制了细胞增长或者说影响了细胞增值周期? 答:不是,完成是改变细胞的代谢。 4)老师,美国FDA公布了一些无血清培养基的配方,我能不能以这个专利配方为基础,进行优化呢?老师能不能给个方向重点优化那些成分呢?比如氨基酸,还有具体怎么优化呢? 答:可以把专利为基础,重点是氨基酸! 5)老师我在专利上查到了IGF-1和转铁蛋白的替代配方?您帮我看看能否可行呢?(具体见附件) 一般都不行,CHO细胞对这两种蛋白都不太敏感 老师,这是领导安排的研究生课题,CHO-S细胞表达重组促卵泡激素无血清培养基的优化,所以必须要作一下探索,我的导师也没做过这方面的工作,我在这方面也没什么方向,所以想请教一下潘老师我该怎么做?我想通过这次实验学到些真本事或者说经验,等毕业了好找工作。老师能不能指导指导我呢?非常非常感谢!! 好好学习!天天向上!
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How much does it cost to do a very small investigational clinical trial of a nonprescription supplement? - Quora

H. Fai Poon's insight:
this is a darn good answer to clinical trial.
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GEN Roundup: How to Improve Cell-Line Productivity | GEN Magazine Articles | GEN

GEN Roundup: How to Improve Cell-Line Productivity | GEN Magazine Articles | GEN | Biopharm | Scoop.it
All too often, the phrase “that dog won’t hunt” applies to cell-line optimization. A productive cell line needs more than a good pedigree, it needs proper care and feeding. Eventually, it may even need to learn new tricks, courtesy of gene editing.
H. Fai Poon's insight:
Erika with the K was former colleague of mine! Here she gave some insight
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GSK orders Sirtris execs to stop selling resveratrol | FierceBiotech

GlaxoSmithKline (NYSE: GSK) has ordered two Sirtris executives--Christoph Westphal and Michelle Dipp--to stop selling a resveratrol dietary supplement based on the drug the two companies have in
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Ascentage Pharma, Unity Biotech collab to ‘cure old age’ | FierceBiotech

Ascentage Pharma, Unity Biotech collab to ‘cure old age’ | FierceBiotech | Biopharm | Scoop.it
East meets West as cancer specialist Ascentage Pharma is teaming up with San Francisco’s Unity Biotechnology to help reverse aging in a new research pact.
H. Fai Poon's insight:
look like aging research is back to spotlight. it has been a decade.
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