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Rockford weight loss journey inspires others

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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Obese people deserve surgical treatment, too

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She came to me before she had gastricbypass surgery t

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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Mother-of-three who shed half of her body weight shares shocking footage of the moment surgeons ...

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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Morning Break: Super Gonorrhea; Pro-Bariatric Surgery; 'Rotting Pelvis'

Compare Affordable Bariatric Surgery Abroad Best Price gastric bypass surgery Morning Break: Super Gonorrhea; Pro-Bariatric Surgery; 'Rotting Pelvis'Note that some links may require subscriptions. Also, tomorrow, March 30, is an official MedPage Today holiday. Morning Break will not appear, but will return on Monday. British man is diagnosed with the first global case of multidrug-resistant gonorrhea, acquired in Southeast Asia. (The Guardian).See all stories on this topic https://icaremedicare.co.uk/bariatrics/morning-break-super-gonorrhea-pro-bariatric-surgery-rotting-pelvis.html/
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Weight Loss - The Best Way To Lose Weight Naturally

Compare Price of Bariatric Surgery Abroad gastric band UK Weight Loss - The Best Way To Lose Weight NaturallyWeight loss has its peculiarities. It can seem like there are many ways to go about it, which is true. Arguing over which is the best method is needless because it is primarily based on opinion. What works for one person is likely going to be their favorite way. Have you found which method works best for you? There is no way around trial and error. At some point, you will need to experiment with different routines: whether it involves diet or physical activity.See all stories on this topic https://icaremedicare.co.uk/bariatrics/weight-loss-the-best-way-to-lose-weight-naturally.html/
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Mom loses 171 pounds walking: 'Life is so much greater when you are healthier'

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Weight-Loss Surgery: Durable in the Long-Term

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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Global Gastric Band Devices Market 2021 Consumption Status, Business Structure, Opportunities ...

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Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study.

Abstract Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS. URO-OVA mice express the membrane form of the model antigen ovalbumin (OVA) as a self-antigen on the urothelium and develop bladder inflammation upon introduction of OVA-specific T cells. To investigate the role of mast cells, we crossed URO-OVA mice with mast cell-deficient KitW-sh mice to generate URO-OVA/KitW-sh mice that retained urothelial OVA expression but lacked endogenous mast cells. We compared URO-OVA mice with URO-OVA/KitW-sh mice with and without mast cell reconstitution in response to cystitis induction. URO-OVA mice developed profound bladder inflammation with increased mast cell counts and LUTD, including increased total number of voids, decreased mean volume voided per micturition, and decreased maximum volume voided per micturition, after cystitis induction. In contrast, similarly cystitis-induced URO-OVA/KitW-sh mice developed reduced bladder inflammation with no mast cells and LUTD detected. However, after mast cell reconstitution URO-OVA/KitW-sh mice restored the ability to develop bladder inflammation and LUTD following cystitis induction. We further treated URO-OVA mice with cromolyn, a mast cell membrane stabilizer, and found that cromolyn treatment reversed bladder inflammation and LUTD in the animal model. Our results provide direct evidence for the role of mast cells in cystitis-associated LUTD, supporting the use of mast cell inhibitors for treatment of certain forms of IC/BPS. Citation: Wang X, Liu W, O'Donnell M, Lutgendorf S, Bradley C, Schrepf A, et al. (2016) Evidence for the Role of Mast Cells in Cystitis-Associated Lower Urinary Tract Dysfunction: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network Animal Model Study. PLoS ONE 11(12): e0168772. https://doi.org/10.1371/journal.pone.0168772 Editor: Jayoung Kim, Cedars-Sinai Medical Center, UNITED STATES Received: October 11, 2016; Accepted: November 16, 2016; Published: December 21, 2016 Copyright: © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the National Institutes of Health, U01DK082344, (https://projectreporter.nih.gov/project_info_details.cfm?aid=9120858&icde=31468144&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC Karl Kreder) and the National Institutes of Health, R01DK100891, (https://projectreporter.nih.gov/project_info_details.cfm?aid=8626530&icde=31468170&ddparam=&ddvalue=&ddsub=&cr=10&csb=default&cs=ASC) to YL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Introduction Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency . IC/BPS patients exhibit an increased number of mast cells in the bladder and elevated levels of mast cell mediators in the https://gastricbandfrance.co.uk/news/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study.html
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Curran: county budget needs gastric bypass

Compare Affordable Weight Loss Surgery Abroad weight loss surgery cost Curran: county budget needs gastric bypassCurran, a Democrat who was elected in November, did not specify the size of the budget gap. “I believe there must be an effort to tighten the belt and undergo our own equivalent of surgical gastric bypass,” Curran said at the meeting of the Nassau County Village Officials Association at Westbury Manor.See all stories on this topic https://icaremedicare.co.uk/bariatrics/curran-county-budget-needs-gastric-bypass.html/
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Widow issues warning after spouse dies of flesh-eating bacteria from raw oysters

Compare Affordable Weight Loss Surgery In Europe Gastric Band Surgery Widow issues warning after spouse dies of flesh-eating bacteria from raw oystersBut those with compromised systems -- LeBlanc, for example, had gastric bypass, which affected her digestion -- are most at risk. According to the CDC, people can become infected with vibrio after eating raw or undercooked shellfish or by exposing open wounds to brackish water. LeBlanc had been in�...See all stories on this topic https://icaremedicare.co.uk/bariatrics/widow-issues-warning-after-spouse-dies-of-flesh-eating-bacteria-from-raw-oysters.html/
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