A Tale of Two Medicines
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A Tale of Two Medicines
Natural Medicine, Pharmaceuticals and GMO’s, the Good, the Bad and the OMG! - (The information provided is not intended to be a substitute for professional medical advice, diagnosis or treatment.  Never disregard professional medical advice, or delay in seeking it, because of something you have read on this scoopit page.)
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Probiotic identified to treat ulcers

Probiotic identified to treat ulcers | A Tale of Two Medicines | Scoop.it

 

Researchers from Spain have identified a strain of probiotic bacteria that may be useful in treating ulcers caused by Helicobacter pylori. They report their findings in the February 2011 issue of the journal Applied and Environmental Microbiology.

 

“H. pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers,” write the researchers. “Currently, antibiotic-based treatment for H. pylori infection is neither sufficient nor satisfactory, with the most successful treatments reaching 75 to 90% eradication rates. The use of probiotics is a potentially promising tool to prevent H. pylori.”

 

According to an expert consultation conducted by the Food and Agriculture Organization and the World Health Organization probiotics are “live microorganisms which when administered in adequate amounts confer a health benefit to the host.” The regular intake of probiotic microoganisms has been demonstrated to prevent several disorders including diarrhea and inflammatory bowel disease.

 

Among probiotics Bifidobacterium is one of the favorite genera in studies focused on the prevention of gastrointestinal infection and is often used in fermented dairy products or food supplements. Some studies have been done in vitro (in test tubes or petri dishes) showing bifidobacterial activity against H. pylori.

 

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Milk is not just food but most likely a genetic transfection system activating mTORC1 signaling for postnatal growth

Milk has been recognized to represent a functionally active nutrient system promoting neonatal growth of mammals. Cell growth is regulated by the nutrient-sensitive kinase mechanistic target of rapamycin complex 1 (mTORC1). There is still a lack of information on the mechanisms of mTORC1 up-regulation by milk consumption. This review presents milk as a materno-neonatal relay system functioning by transfer of preferential amino acids, which increase plasma levels of glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1), insulin, growth hormone (GH) and insulin-like growth factor-1 (IGF-1) for mTORC1 activation. Importantly, milk exosomes, which regularly contain microRNA-21, most likely represent a genetic transfection system enhancing mTORC1-driven metabolic processes. Whereas human breast milk is the ideal food for infants allowing appropriate postnatal growth and species-specific metabolic programming, persistent high milk signaling during adolescence and adulthood by continued cow´s milk consumption may promote mTORC1-driven diseases of civilization.
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Christian Yamashiba Kasongo's curator insight, March 17, 2014 12:23 PM

Le lait n'est pas seulement la nourriture mais plus probablement un système de transfection génétique activation mTORC1 de signalisation pour la croissance postnatale points AJOUTER de Votre de vue ...

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Investigations of kanuka and manuka essential oils for in vitro treatment of disease and cellular inflammation caused by infectious microorganisms

Diseases caused by infectious and inflammatory microorganisms are among the most common and most severe nosocomial diseases worldwide. Therefore, developing effective agents for treating these illnesses is critical. In this study, essential oils from two tea tree species, kanuka (Kunzea ericoides) and manuka (Leptospermum scoparium), were evaluated for use in treating diseases and inflammation caused by microorganism infection.

 

Isolates of clinically common bacteria and fungi were obtained from American Type Culture Collection and from Kaohsiung Veterans General Hospital. Minimum inhibitory concentrations for Trichosporon mucoides, Malassezia furfur, Candida albicans, and Candida tropicalis were determined by the broth microdilution method with Sabouraud dextrose broth. The antibacterial susceptibility of Staphylococcus aureus, Streptococcus sobrinus, Streptococcus mutans, and Escherichia coli were determined by the broth microdilution method. A human acute monocytic leukemia cell line (THP-1) was cultured to test the effects of the essential oils on the release of the two inflammatory cytokines, tumor necrosis factor-α and interleukin-4.

 

Multiple analyses of microorganism growth confirmed that both essential oils significantly inhibited four fungi and the four bacteria. The potent fungicidal properties of the oils were confirmed by minimum inhibitory concentrations ranging from 0.78% to 3.13%. The oils also showed excellent bactericidal qualities with 100% inhibition of the examined bacteria. In THP-1 cells, both oils lowered tumor necrosis factor-α released after lipopolysaccharide stimulation. Finally, the antimicrobial and anti-inflammatory effects of the oils were obtained without adversely affecting the immune system.

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Christian Yamashiba Kasongo's curator insight, March 17, 2014 12:26 PM

Une des Huiles Essentielles Pour Le Traitement in vitro de la maladie et de l'inflammation cellulaire Causee par des micro-organismes de la jouter Votre Point de Vue de ... Enquêtes de Kanuka et des huiles essentielles de manuka pour le traitement in vitro de la maladie et de l'inflammation cellulaire causée par des microorganismes infectieux

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Synergistic Antioxidant Activity of Sweet Potato Extracts in Combination with Tea Polyphenols and Pueraria Flavonoid in Vitro

Synergistic Antioxidant Activity of Sweet Potato Extracts in Combination with Tea Polyphenols and Pueraria Flavonoid in Vitro | A Tale of Two Medicines | Scoop.it

The antioxidant activity of Sweet Potato Extracts (SPE) can be enhanced by the presence of these other active antioxidants such as Tea Polyphones (TP) and Pueraria Flavonoid (PF). Since many of these natural antioxidants are consumed together in foods, the potential for synergistic interactions is high in the human diet. The aim of this study was to determine what concentrations and combinations of antioxidants among SPE, TP and PF are capable of producing synergistic antioxidant effects, based on potato-based food products. Solutions of the antioxidant activity of SPE, TP and PF, alone and in different combinations were measured using the stable free radical 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and Ferric Reducing Anti-oxidant Power (FRAP) method. A comparison of the antioxidant activity of the combinations of antioxidants to the arithmetic sum of the antioxidant activity of the individual antioxidants was used to calculate the Synergistic Effects (SEs) between the antioxidants. The results showed that all concentrations of TP and PF combination with SPE (1 and 1.5%) could produce significant SEs (p<0.05) of DPPH and FRAP for the two or three component mixtures. With the concentration of 1% SPE, 1×10-5 g/mL TP and 5×10-5 g/mL PF in the three-component mixture, the highest SE of DPPH and FRAP was both detected. The results suggested that the antioxidant property of this combination was substantially superior to the sum of the individual antioxidant effects and these interactions can enhance the antioxidant effectiveness of SPE. The results could guide in the formulation and development of functional food products that have high antioxidant potential.

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Unexpected link between an antibiotic, pannexin channels and apoptosis.

Unexpected link between an antibiotic, pannexin channels and apoptosis. | A Tale of Two Medicines | Scoop.it

Plasma membrane pannexin 1 channels (PANX1) release nucleotide find-me signals from apoptotic cells to attract phagocytes. Here we show that the quinolone antibiotic trovafloxacin is a novel PANX1 inhibitor, by using a small-molecule screen. Although quinolones are widely used to treat bacterial infections, some quinolones have unexplained side effects, including deaths among children. PANX1 is a direct target of trovafloxacin at drug concentrations seen in human plasma, and its inhibition led to dysregulated fragmentation of apoptotic cells. Genetic loss of PANX1 phenocopied trovafloxacin effects, revealing a non-redundant role for pannexin channels in regulating cellular disassembly during apoptosis. Increase in drug-resistant bacteria worldwide and the dearth of new antibiotics is a major human health challenge. Comparing different quinolone antibiotics suggests that certain structural features may contribute to PANX1 blockade. These data identify a novel linkage between an antibiotic, pannexin channels and cellular integrity, and suggest that re-engineering certain quinolones might help develop newer antibacterials.

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Curcumin Micelle Bioavailability Is “Unrivaled,” Study Researchers Say

Curcumin Micelle Bioavailability Is “Unrivaled,” Study Researchers Say | A Tale of Two Medicines | Scoop.it

 

Curcumin delivered in aqueous micelles achieved “unrivaled” bioavailability results in a new study published in the journal Molecular Nutrition & Food Research. The study compared the bioavailability of a native curcumin powder to the more bioavailable formats of a micronized curcumin powder or curcumin micelles.

 

Curcumin is lipophilic, making it poorly water soluble. Micronization and micelles are two approaches used in drug delivery to enhance bioavailability. Micronization is the reduction of particle size. Micelles are lipid molecules that act as a carrier for lipophilic ingredients like curcumin, making them more soluble.

 

The Molecular Nutrition & Food Research study was a 23-subject crossover trial comprising three study arms, each separated by a one-week washout period. Subjects were given a single, 500-mg dose of curcuminoids in either a native curcumin powder, micronized powder, or liquid micelles, mixed into 50 g of syrup.

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Getting Back To Basics...How Low-Cost Zinc Helps Combat Deadly Immunosenescence

Getting Back To Basics...How Low-Cost Zinc Helps Combat Deadly Immunosenescence | A Tale of Two Medicines | Scoop.it

 

 

Zinc is required by the body for more than 2,000 transcription factors involved in gene expressions of various proteins.What this means in everyday language is that thousands of essential biological functions are dependent on zinc.

 

The medical community has known of zinc deficiency for more than 50 years, but the health impact of this crucial mineral has been largely ignored by global health organizations. Extensive scientific inquiry has made it clear that nutritional deficiency of zinc is widely prevalent and its morbidities are severe.

 

Overwhelmingly, the elderly are deficient in zinc. Because zinc governs so many biological functions, a simple zinc deficiency can affect multiple facets of health and development. The result is a decline in our body’s vigilant immune system, opening the door for an onslaught of numerous diseases. A zinc deficiency contributes to atherosclerosis, cancer, neurological disorders, autoimmune diseases, and other age-related chronic conditions.

 

One target that researchers are focusing on is the fact that a zinc deficiency can cause your immune system to decline, a phenomenon known as immunosenescence. This decline in immunity places older adults at increased risk for a range of almost every serious disease, from infections and cancer.

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Anti-inflammatory effects of anthocyanins-rich extract from bilberry (Vaccinium myrtillus L.) on croton oil-induced ear edema and Propionibacterium acnes plus LPS-induced liver damage in mice, Inte...

Anti-inflammatory effects of anthocyanins-rich extract from bilberry (Vaccinium myrtillus L.) on croton oil-induced ear edema and Propionibacterium acnes plus LPS-induced liver damage in mice, Inte... | A Tale of Two Medicines | Scoop.it
Bilberry (Vaccinium myrtillus L.) has been known to play a protective role in human health due to its high anthocyanin content. This study investigated the anti-inflammatory effects of bilberry extract (BE, containing 42.04% anthocyanin) on Propionibacterium acnes (P. acnes) plus lipopolysaccharide (LPS) induced liver injury and croton oil-induced ear edema in mice. Results showed that BE could effectively inhibit croton oil-induced ear edema and liver inflammation provoked by P. acnes plus LPS, as reflected by the reduced plasma alanine aminotransferase and aspartate aminotransferase activities. These findings were confirmed by hepatic pathological examination. Moreover, BE administration markedly suppressed the increase of liver mRNA levels of iNOS, TNF-α, IL-1β and IL-6, and the protein levels of iNOS, TNF-α and NF-κB. In addition, liver malondialdehyde and NO contents were significantly reduced by BE treatment. These results indicated that BE has potent protective effects on acute and immunological inflammation, which might contribute to the study of the anti-inflammatory effects of natural products and healthy food.

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Christian Yamashiba Kasongo's curator insight, March 11, 2014 4:20 PM

Anti-inflammatory effects of anthocyanins-rich extract from bilberry (Vaccinium myrtillus L.) on croton oil-induced ear edema and Propionibacterium acnes plus LPS-induced liver damage in mice, Inte...ajouter votre point de vue ...

Christian Yamashiba Kasongo's curator insight, March 13, 2014 4:33 PM

Anti-inflammatory effects of anthocyanins-rich extract from bilberry (Vaccinium myrtillus L.) on croton oil-induced ear edema and Propionibacterium acnes plus LPS-induced liver damage in mice, Inte...         

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Inhibitory Effects of Wild Blueberry Anthocyanins and Other Flavonoids on Biomarkers of Acute and Chronic Inflammation in Vitro.

Inhibitory Effects of Wild Blueberry Anthocyanins and Other Flavonoids on Biomarkers of Acute and Chronic Inflammation in Vitro. | A Tale of Two Medicines | Scoop.it

Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.

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Pathogens Hijack the Epigenome: A New Twist on Host-Pathogen Interactions

Pathogens have evolved strategies to promote their survival by dramatically modifying the transcriptional profile and protein content of the host cells they infect. Modifications of the host transcriptome and proteome are mediated by pathogen-encoded effector molecules that modulate host cells through a variety of different mechanisms. Recent studies highlight the importance of the host chromatin and other epigenetic regulators as targets of pathogens. Host gene regulatory mechanisms may be targeted through cytoplasmic signaling, directly by pathogen effector proteins, and possibly by pathogen RNA. Although many of these changes are short-lived and persist only during the course of infection, several studies indicate that pathogens are able to induce long-term, heritable changes that are essential to pathogenesis of infectious diseases and persistence of pathogens within their hosts. In this review, we discuss how pathogens modulate the epigenome of host cells, a new and flourishing avenue of host-pathogen interaction studies.
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Cancer stem cells: potential target for bioactive food components.

Cancer stem cells often have phenotypic and functional characteristics similar to normal stem cells including the properties of self-renewal and differentiation. Recent findings suggest that uncontrolled self-renewal may explain cancer relapses and may represent a critical target for cancer prevention. It is conceivable that the loss of regulatory molecules resulting from inappropriate consumption of specific foods and their constituents may foster the aberrant self-renewal of cancer stem cells. In fact, increasing evidence points to the network delivering signals for self-renewal from extracellular compartments to the nucleus including changes in stem cell environments, inducible expression of microRNAs, hyperplastic nuclear chromatin structures, and the on/off of differentiation process as possible sites of action for bioactive food components. Diverse dietary constituents such as vitamins A and D, genistein, (-)-epigallocatechin-3-gallate (EGCG), sulforaphane, curcumin, piperine, theanine and choline have been shown to modify self-renewal properties of cancer stem cells. The ability of these bioactive food components to influence the balance between proliferative and quiescent cells by regulating critical feedback molecules in the network including dickkopf 1 (DKK-1), secreted frizzled-related protein 2 (sFRP2), B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) and cyclin-dependent kinase 6 (CDK6) may account for their biological response. Overall, the response to food components does not appear to be tissue or organ specific, suggesting there may be common cellular mechanisms. Unquestionably, additional studies are needed to clarify the physiological role of these dietary components in preventing the resistance of tumor cells to traditional drugs and cancer recurrence.

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One-year supplementation with a grape extract containing resveratrol modulates inflammatory-related microRNAs and cytokines expression in peripheral blood mononuclear cells of type 2 diabetes and h...

Numerous studies have shown that resveratrol (RES) exerts anti-inflammatory effects but human trials evidencing these effects in vivo are limited. Furthermore, the molecular mechanisms triggered in humans following the oral intake of RES are not yet understood. Therefore, the purpose of this study was to investigate the molecular changes in peripheral blood mononuclear cells (PBMCs) associated to the one-year daily intake of a RES enriched (8 mg) grape extract (GE-RES) in hypertensive male patients with type 2 diabetes mellitus (T2DM). We used microarrays and RT-PCR to analyze expression changes in genes and microRNAs (miRs) involved in the inflammatory response modulated by the consumption of GE-RES in comparison to a placebo and GE lacking RES. We also examined the changes in several serobiochemical variables, inflammatory and fibrinolytic markers. Our results showed that supplementation with GE or GE-RES did not affect body weight, blood pressure, glucose, HbA1c or lipids, beyond the values regulated by gold standard medication in these patients. We did not find either any significant change on serum inflammatory markers except for a significant reduction of ALP and IL-6 levels. The expression of the pro-inflammatory cytokines CCL3, IL-1β and TNF-α was significantly reduced and that of the transcriptional repressor LRRFIP-1 increased in PBMCs from patients taking the GE-RES extract. Also, a group of miRs involved in the regulation of the inflammatory response: miR-21, miR-181b, miR-663, miR-30c2, miR-155 and miR-34a were found to be highly correlated and altered in the group consuming the GE-RES for 12 months. Our results provide preliminary evidence that long-term supplementation with a grape extract containing RES downregulates the expression of key pro-inflammatory cytokines with the involvement of inflammation-related miRs in circulating immune cells of T2DM hypertensive medicated patients and support a beneficial immunomodulatory effect in these patients.

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Condurango glycoside-rich components stimulate DNA damage-induced cell cycle arrest and ROS-mediated caspase-3 dependent apoptosis through inhibition of cell-proliferation in lung cancer, in vitro ...

Chemotherapeutic potential of Condurango glycoside-rich components (CGS) was evaluated in NSCLC, in vitro and in BaP-intoxicated rats, in vivo. NSCLC cells were treated with different concentrations of CGS to test their effect on cell viability. Cellular morphology, DNA-damage, AnnexinV-FITC/PI, cell cycle regulation, ROS-accumulation, MMP, and expressions of related signalling genes were critically analysed. 0.22 μg/μl CGS (IC50 dose at 24 h) was selected for the study. CGS-induced apoptosis via DNA damage was evidenced by DNA-ladder formation, increase of AnnexinV-positive cells, cell cycle arrest at subG0/G1 and differential expressions of apoptotic genes. ROS-elevation and MMP-depolarization with significant caspase-3 activation might lead to apoptotic cell death. Anti-proliferative activity was confirmed by EGFR-expression modulation. ROS accumulation and DNA-nick formation with tissue damage-repair activity after post-cancerous CGS treatment, in vivo, supported the in vitro findings. Overall results advocate considerable apoptosis-inducing potential of CGS against NSCLC, validating its use against lung cancer by CAM practitioners.

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The Saturated Fat, Cholesterol, and Statin Controversy A Commentary

The Saturated Fat, Cholesterol, and Statin Controversy A Commentary | A Tale of Two Medicines | Scoop.it

 

In the 1930s, there were advertisements run in JAMA with testimonials from MDs saying that smoking cigarettes did not harm your lungs. It took many years of increasing lung cancer incidence to convince people that this was not a true statement. The current situation with serum cholesterol and coronary heart disease is very similar in concept, though this time doctors are widely convinced that the former causes the latter, despite a glaring lack of scientific evidence. There have been no studies that prove dietary cholesterol levels directly cause atherosclerosis. There are many correlation studies that may suggest an association but none that show cause and effect. In fact, if analyzed carefully, there are more studies that disprove the “cause and effect” hypothesis than even come close to proving it. One basic tenant of biostatistics and epidemiologic research is that you can never prove a hypothesis - you can only fail to disprove it. Experimental design has three essential components: 1) Estimate of error, 2) Control of error and 3) Proper interpretation of results [1]. Correlation studies indicate a possible relationship between two factors, i.e, blood cholesterol and CVD, but they do not prove cause and effect. Correlations are often observed in epidemiological studies, but carefully designed studies with sufficient control of confounding factors and avoidance of other potential bias needs to be conducted to demonstrate cause and effect. In these cases, small differences in the design of the study can cause different outcomes. Most of the studies in animal models are not a good model to compare the same systems as in humans. They may give hints, but they ultimately need to be tested in humans. Many of the early studies were used rabbits feeding a high cholesterol diet. Rabbits in the wild never eat cholesterol since they are herbivores and plants do not make appreciable cholesterol, so the animals would not normally eat cholesterol in their diet. Additionally, the cholesterol consumed by the test animals contained “bare” cholesterol, chemically prepared and added to the diets, so quite likely was oxidized by the time the animals ate it. The cholesterol in human diets is a component of a food tissue and often bound with a fatty acid and protected from oxidation. Rodent models are not a good model for humans since most of them have high HDL and very little LDL as opposed to humans who have more LDL than HDL in the blood. So feeding rabbits a high cholesterol diet and rendering them athrogenic does not prove causality.  

 

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Christian Yamashiba Kasongo's curator insight, March 17, 2014 12:19 PM

C:\Users\Christian\Epi Info 7\Projects\Nutrition\6ème Université promosanté Dakar.MDBajouter votre point de vue ...

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One in five older Americans take medications that work against each other

One in five older Americans take medications that work against each other | A Tale of Two Medicines | Scoop.it

About three out of four older Americans have multiple chronic health conditions, and more than 20 percent of them are being treated with drugs that work at odds with each other – the medication being used for one condition can actually make the other condition worse.

 

This approach of treating conditions “one at a time” even if the treatments might conflict with one another is common in medicine, experts say, in part because little information exists to guide practitioners in how to consider this problem, weigh alternatives and identify different options.

 

One of the first studies to examine the prevalence of this issue, however, found that 22.6 percent of study participants received at least one medication that could worsen a coexisting condition. The work was done by researchers in Connecticut and Oregon, and published in PLOS One.

In cases where this “therapeutic competition” exists, the study found that it changed drug treatments in only 16 percent of the cases. The rest of the time, the competing drugs were still prescribed.

 

“Many physicians are aware of these concerns but there isn’t much information available on what to do about it,” said David Lee, an assistant professor in the Oregon State University/Oregon Health & Science University College of Pharmacy.

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Wild mushroom extracts potentiate the action of standard antibiotics against multiresistant bacteria.

Wild mushroom extracts potentiate the action of standard antibiotics against multiresistant bacteria. | A Tale of Two Medicines | Scoop.it

The main objective of this study was to evaluate the capacity of wild mushroom extracts to potentiate the action of standard antibiotics, through synergisms that allow a decrease in their therapeutic doses and ultimately contribute to the reduction of resistances.

 

Wild mushroom extracts were applied to different multiresistant micro-organisms (Escherichia coli, extended-spectrum beta-lactamase-producing (ESBL) E. coli and methicillin-resistant Staphylococcus aureus (MRSA), combined with commercial antibiotics (penicillin, ampicillin, amoxicillin/clavulanic acid, cefoxitin, ciprofloxacin, cotrimoxazol, levofloxacin). Microdilution method was used to determine minimum inhibitory concentrations (MICs). The results obtained showed higher synergistic effects against MRSA than against E. coli. Mycena rosea and Fistulina hepatica were the best extracts for synergistic effects against MRSA. The efficiency of Russula delica extract against E. coli 1 (resistant to ampicillin, ciprofloxacin and trimethoprim/sulfasoxazole) and E. coli 2 (resistant to amoxicillin/clavulanic acid and ampicillin) was higher than that of Leucopaxillus giganteus extract; nevertheless, the latter extract exhibited better synergistic effects against ESBL E. coli.

 

This study shows that, similarly to plants, some mushroom extracts can potentiate the action of antibiotics extensively used in clinical practice for Gram-positive or Gram-negative bacteria, with positive action even against multiresistant bacteria.

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Christian Yamashiba Kasongo's curator insight, March 17, 2014 12:27 PM

ajo extraits de champignons sauvages potentialisent l'action des antibiotiques standards contre les bactéries multirésistantes. uter Votre Point de Vue ...

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Natural Herbal HPV "Cure" Discovered

Natural Herbal HPV "Cure" Discovered | A Tale of Two Medicines | Scoop.it

Despite the widespread belief that HPV infection is a lethal force against which we only have vaccination and watchful waiting to defend ourselves, both ancient herbal medicine and our body's inherent immune defenses have newly been confirmed to have significant power against it.


A groundbreaking study published in the Asian Pacific Journal of Cancer Prevention, titled, "Clearance of Cervical Human Papillomavirus Infection by Topical Application of Curcumin and Curcumin Containing Polyherbal Cream: A Phase II Randomized Controlled Study," reveals that vaccination and watchful waiting are not the only recourse against HPV infection.  

 

The study is believed to be the first of its kind to find an effective and safe therapeutic intervention for the clearance of established cervical human papillomavirus (HPV) infection. Moreover, the study confirmed that HPV infection is self-limiting and clears on its own in 73.3% of the untreated placebo group within 37 days. 

 

The researchers evaluated the effectiveness of two herbal interventions in eliminating HPV infection from the cervix of women who were determined to have HPV infection through Pap smear and HPV DNA tests (PCR), but whose condition had not yet progressed to high grade cervical neoplasias (i.e. cervical pre-cancer).

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Effects of coenzyme Q10 supplementation on antioxidation and anti-inflammation in coronary artery disease patients during statins therapy.

Effects of coenzyme Q10 supplementation on antioxidation and anti-inflammation in coronary artery disease patients during statins therapy. | A Tale of Two Medicines | Scoop.it

In this clinical trial, we have demonstrated that coenzyme Q10 at the dose of 300 mg/d for 12 weeks increases the antioxidant enzymes activities (SOD, CAT, and GPx) and decreases inflammation in patients with CAD during statins therapy. Tiano et al. administered coenzyme Q10 (300 mg/d) to patients with ischemic heart disease for 1 month, and they observed that those patients’ extracellular superoxide dismutase activity and endothelium-dependent vasodilatation were improved after supplementation. Antioxidant enzymes such as SOD, CAT, and GPx are the first line of defense against reactive oxygen species, and a decrease in their activities contributes to the elevated oxidative stress in CAD patients. Our previous results showed that coenzyme Q10 at a dose of 150 mg/d increased the activity of SOD by 22.2% and of CAT by 4.5%, but had no effect on that of GPx. In the present study, coenzyme Q10 supplementation at 300 mg/d increased the activity of SOD by 48.5%, CAT by 9.1%, and GPx by 4.3%, and the antioxidant enzymes activities were all positively correlated with the level of coenzyme Q10 after 12 weeks of supplementation. It appears that the 300 mg dose of coenzyme Q10 has better antioxidation than 150 mg/d.

 

Both groups of statins-treated CAD patients in this study had a low level of coenzyme Q10 at baseline, and the level of coenzyme Q10 was significantly increased by approximately 5-fold after 4 weeks of coenzyme Q10 supplementation (data not shown; the median level of coenzyme Q10 was 0.4 to 2.0 μmol/L), which rapidly adjusted their low coenzyme Q10 level to normal values (0.5 – 1.7 μmol/L). In addition, coenzyme Q10 had a synergic effect with vitamin E . In this study, we observed that the level of vitamin E was significantly higher in the Q10-300 group and was positively correlated with the level of coenzyme Q10 after 12 weeks of supplementation. Coenzyme Q10 not only protects vitamin E against superoxide-driven oxidation but also regenerates vitamin E during antioxidation processes

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Christian Yamashiba Kasongo's curator insight, March 13, 2014 4:26 PM

Effects of coenzyme Q10 supplementation on antioxidation and anti-inflammation in coronary artery disease patients during statins therapy.ajouter votre point de vue ...

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Effect of broccoli sprouts on insulin resistance in type 2 diabetic patients: a randomized double-blind clinical trial.

Use of antioxidant components is a new approach for improvement of insulin resistance (IR) as a main feature of type 2 diabetes and its complications. The aim of this study was to investigate the effects of broccoli sprouts powder (BSP) containing high concentration of sulphoraphane on IR in type 2 diabetic patients. Eighty-one patients were randomly assigned to receive 10 g/d BSP (A, n = 27), 5 g/d BSP (B, n = 29) and placebo (C, n = 25) for 4 weeks. Fasting serum glucose and insulin concentration, glucose to insulin ratio and homoeostasis model assessment of IR (HOMA-IR) index were measured at baseline and again 4 weeks after treatment. Seventy-two patients completed the study and 63 were included in the analysis. After 4 weeks, consumption of 10 g/d BSP resulted in a significant decrease in serum insulin concentration and HOMA-IR (p = 0.05 for treatment effect). Therefore, broccoli sprouts may improve IR in type 2 diabetic patients.

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Christian Yamashiba Kasongo's curator insight, March 11, 2014 4:14 PM

Effet de pousses de brocoli sur la résistance à l'insuline de type 2 chez les patients diabétiques: un double aveugle essai clinique randomisé points AJOUTER de Votre de vue ...

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Antioxidant, Anti-inflammatory and Antinociceptive Properties of Black Pepper Essential Oil (Piper nigrum Linn)

Antioxidant, Anti-inflammatory and Antinociceptive Properties of Black Pepper Essential Oil (Piper nigrum Linn) | A Tale of Two Medicines | Scoop.it

This study is aimed to investigate the chemical composition of essential oil isolated from fruits of Piper nigrum L. (black pepper) by GC-MS and to study its pharmacological properties. The main constituents identified were caryophyllene (23.98 %) and limonene (14.36 %). Black pepper oil scavenged superoxide, DPPH, hydroxyl radicals and inhibited tissue lipid peroxidation in vitro. Intraperitoneal administration of essential oil was found to inhibit PMA induced super oxide radicals elicited by macrophages. Oral administration of black pepper oil for one month, significantly increased superoxide dismutase, glutathione and glutathione reductase enzyme levels in blood of mice and glutathione-S-transferase, glutathione peroxidase, catalase, superoxide dismutase and glutathione enzymes in liver. Black pepper oil significantly reduced acute inflammation induced by carrageenan and dextran and formalin induced chronic models of inflammation. It also exhibited antinociceptive property in acetic acid induced writhing test. These studies reveal that black pepper essential oil possesses antioxidant, anti-inflammatory and antinociceptive property.

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Christian Yamashiba Kasongo's curator insight, March 13, 2014 4:31 PM

Antioxidant, Anti-inflammatory and Antinociceptive Properties of Black Pepper Essential Oil (Piper nigrum Linn)ajouter votre point de vue ...

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Doxorubicin-induced oxidative stress in rats is efficiently counteracted by dietary anthocyanins-differently-enriched strawberry (Fragaria x ananassa Duch.)

Doxorubicin-induced oxidative stress in rats is efficiently counteracted by dietary anthocyanins-differently-enriched strawberry (Fragaria x ananassa Duch.) | A Tale of Two Medicines | Scoop.it

This study investigated the effects of two different strawberry cultivars, Adria and Sveva, against Doxorubicin (DOX)-induced toxicity in rats. A controlled dietary intervention was conducted over 16 weeks with four groups: (i) normal diet, (ii) normal diet+DOX injection, (iii) Adria supplementation+DOX injection and (iv) Sveva supplementation+DOX injection. Sveva presented a higher total antioxidant capacity value, phenol and Vitamin C levels than Adria, that in turn presented higher anthocyanin contents. DOX drastically increased lymphocytes DNA damage, liver biomarkers of protein and lipid oxidation, mitochondrial ROS content and markedly decreased plasma retinol level, liver antioxidant enzymes and mitochondrial functionality. After four months of strawberry supplementation, rats presented a significant reduction of DNA damage and ROS concentration, and a significant improvement of oxidative stress biomarkers, antioxidant enzyme activities and mitochondrial performance. Our results suggest that strawberry supplementation can counteract DOX toxicity, confirming the potential health benefit of strawberry in vivo against oxidative stress.

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Green Tea and One of Its Constituents, Epigallocatechine-3-gallate, Are Potent Inhibitors of Human 11β-hydroxysteroid Dehydrogenase Type 1

Green Tea and One of Its Constituents, Epigallocatechine-3-gallate, Are Potent Inhibitors of Human 11β-hydroxysteroid Dehydrogenase Type 1 | A Tale of Two Medicines | Scoop.it

 

Tea infusions have not only been a popular beverage for thousands of years but also have been traditionally used for the treatment of the metabolic syndrome and other chronic diseases. The metabolic syndrome is a complex metabolic disorder that may lead to diabetes type 2 and is one of the fastest growing diseases within the western civilisation. The role of glucocorticoid hormones in maintaining the blood glucose level by mobilization of energy sources is essential for homeostasis: Cortisol deficiency results in low blood glucose levels, dizziness, generalized weakness and stress sensitivity while cortisol overproduction, hypercortisolism, leads to high blood pressure, increased abdominal fat deposition, insulin resistance etc. Due to its pivotal role in cortisol production, inhibition of 11β-HSD1 maybe of therapeutic value in the treatment of the metabolic syndrome and diabetes.

 

For these reasons our laboratory has investigated the inhibitory effects of tea, in particular green tea and some of its constitutents, on microsomal and purified 11β-HSD1 mediated cortisone reductase (and cortisol dehydrogenase) activity. The present study demonstrates that tea and green tea, respectively, and some of their polyphenolic constituents, EGCG and GC, have the capability to inhibit human 11β-HSD1.

 

Initially, we could show that non-fermented green tea, semi-fermented tea (e.g. oolong tea and white tea) and fermented black tea have different potencies to inhibit human 11β-HSD1 mediated cortisone reduction. Non-fermented green tea showed the highest inhibitory potential (IC50 of 3.75 mg crushed dried tea leaves per ml, which corresponds to a value of 0.75 vol% of green tea extract), whereas a weaker inhibition potency was observed by semi-fermented white and fermented black tea.

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A diet rich in high-glucoraphanin broccoli interacts with genotype to reduce discordance in plasma metabolite profiles by modulating mitochondrial function

Observational and experimental studies suggest that diets rich in cruciferous vegetables and glucosinolates may reduce the risk of cancer and cardiovascular disease (CVD).

 

We tested the hypothesis that a 12-wk dietary intervention with high-glucoraphanin (HG) broccoli would modify biomarkers of CVD risk and plasma metabolite profiles to a greater extent than interventions with standard broccoli or peas.

 

Subjects were randomly assigned to consume 400 g standard broccoli, 400 g HG broccoli, or 400 g peas each week for 12 wk, with no other dietary restrictions. Biomarkers of CVD risk and 347 plasma metabolites were quantified before and after the intervention.

 

No significant differences in the effects of the diets on biomarkers of CVD risk were found. Multivariate analyses of plasma metabolites identified 2 discrete phenotypic responses to diet in individuals within the HG broccoli arm, differentiated by single nucleotide polymorphisms associated with the PAPOLG gene. Univariate analysis showed effects of sex (P < 0.001), PAPOLG genotype (P < 0.001), and PAPOLG genotype × diet (P < 0.001) on the plasma metabolic profile. In the HG broccoli arm, the consequence of the intervention was to reduce variation in lipid and amino acid metabolites, tricarboxylic acid (TCA) cycle intermediates, and acylcarnitines between the 2 PAPOLG genotypes.

 

The metabolic changes observed with the HG broccoli diet are consistent with a rebalancing of anaplerotic and cataplerotic reactions and enhanced integration of fatty acid β-oxidation with TCA cycle activity. These modifications may contribute to the reduction in cancer risk associated with diets that are rich in cruciferous vegetables.

Jonathan Middleton's insight:

"A diet rich in cruciferous vegetables effectively retunes our metabolism by rebalancing anaplerosis and cataplerosis and restoring metabolic homeostasis. In this manner, cruciferous vegetables may be able to reduce the risk of many chronic diseases associated with aging."

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Targeting breast stem cells with the cancer preventive compounds curcumin and piperine.

The cancer stem cell hypothesis asserts that malignancies arise in tissue stem and/or progenitor cells through the dysregulation or acquisition of self-renewal. In order to determine whether the dietary polyphenols, curcumin, and piperine are able to modulate the self-renewal of normal and malignant breast stem cells, we examined the effects of these compounds on mammosphere formation, expression of the breast stem cell marker aldehyde dehydrogenase (ALDH), and Wnt signaling. Mammosphere formation assays were performed after curcumin, piperine, and control treatment in unsorted normal breast epithelial cells and normal stem and early progenitor cells, selected by ALDH positivity. Wnt signaling was examined using a Topflash assay. Both curcumin and piperine inhibited mammosphere formation, serial passaging, and percent of ALDH+ cells by 50% at 5 mu M and completely at 10 mu M concentration in normal and malignant breast cells. There was no effect on cellular differentiation. Wnt signaling was inhibited by both curcumin and piperine by 50% at 5 mu M and completely at 10 mu M. Curcumin and piperine separately, and in combination, inhibit breast stem cell self-renewal but do not cause toxicity to differentiated cells. These compounds could be potential cancer preventive agents. Mammosphere formation assays may be a quantifiable biomarker to assess cancer preventive agent efficacy and Wnt signaling assessment can be a mechanistic biomarker for use in human clinical trials.

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Plymouth researchers find chemical in red grapes could help symptoms of rare childhood disease

Plymouth researchers find chemical in red grapes could help symptoms of rare childhood disease | A Tale of Two Medicines | Scoop.it

 

PLYMOUTH researchers have said a chemical found in red fruits - and even red wine - could reduce the symptoms of a rare childhood muscle disease. Scientists from Plymouth University Peninsula Schools of Medicine and Dentistry have been part of an international team led by the University of Edinburgh, who said the chemical could reduce the symptoms of a muscle disease which leaves children with little or no control of their movements.

 

Their study is published today in the Journal of Clinical Investigation.

The research team have found that a plant pigment called quercetin – present in some fruits, vegetables, herbs and grains – could help to prevent damage to the nerves associated with the childhood form of motor neurone disease, spinal muscular atrophy (SMA).

 

Red grapes, blueberries, blackberries and red cherries are among the best sources of quercetin - even red wine contains the protective antioxidants.

 

SMA, also known as ‘floppy baby syndrome’, is a leading genetic cause of death in children.

 

It affects one in 6,000 to 10,000 children and around half of children with the most severe form will die before the age of two.



 

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