Plasmodiophora brassicae and Clubroot
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Plasmodiophora brassicae and Clubroot
Plasmodiophora brassicae
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Commentaries and Letters to the Editor of The Plant Cell on recognition specificity of the FLS2 receptor (2012)

Does the Arabidopsis receptor FLS2 respond to CLV3 and Ax21 peptides? "The editors of The Plant Cell feel that they have taken responsibility for opening up the scientific debate on FLS2 and innate immune signaling, and they have gone as far as possible to ensure a fair and balanced discussion of issues of particular interest to its readership":

 

Cathie Martin. Commentaries and Letters to the Editor of The Plant Cell http://tinyurl.com/d6mgbmf

 

Katharina Mueller, Delphine Chinchilla, Markus Albert, Anna K. Jehle, Hubert Kalbacher, Thomas Boller, and Georg Felix. Contamination Risks in Work with Synthetic Peptides: flg22 as an Example of a Pirate in Commercial Peptide Preparations http://tinyurl.com/c2u3pr6

 

Cécile Segonzac, Zachary L. Nimchuk, Martina Beck, Paul T. Tarr, Silke Robatzek, Elliot M. Meyerowitz, and Cyril Zipfel. The Shoot Apical Meristem Regulatory Peptide CLV3 Does Not Activate Innate Immunity http://tinyurl.com/bslnq4o

 

Horim Lee, Ashok Khatri, Julia M. Plotnikov, Xue-Cheng Zhang, and Jen Sheen. Complexity in Differential Peptide–Receptor Signaling: Response to Segonzac et al. and Mueller et al. Commentaries http://tinyurl.com/c2csqm3

 

Cristian H. Danna, Xue-Cheng Zhang, Ashok Khatri, Andrew F. Bent, Pamela C. Ronald, and Frederick M. Ausubel. FLS2-Mediated Responses to Ax21-Derived Peptides: Response to the Mueller et al. Commentary http://tinyurl.com/d2a36cr

 

Mueller et al. also used the CLV3 peptide as negative control in this Plant cell paper: http://tinyurl.com/bszkm24

 

These are the two contested papers:

 

Danna, C.H., Millet, Y.A., Koller, T., Han, S.W., Bent, A.F., Ronald, P.C., and Ausubel, F.M. (2011). The Arabidopsis flagellin receptor FLS2 mediates the perception of Xanthomonas Ax21 secreted peptides. Proc. Natl. Acad. Sci. USA 108: 9286–9291. http://tinyurl.com/brwkb4w

 

Lee, H., Chah, O.-K., and Sheen, J. (2011). Stem-cell-triggered immunity through CLV3p-FLS2 signalling. Nature 473: 376–379. http://tinyurl.com/3thgqut


Via Kamoun Lab @ TSL, alex, Guogen Yang
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Rescooped by yingzhao from PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
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In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR

In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR | Plasmodiophora brassicae and Clubroot | Scoop.it

A protocol using real-time polymerase chain reaction (PCR) for the direct detection and quantification of Plasmodiophora brassicae in soil samples was developed and used on naturally and artificially infested soil samples containing different concentrations of P. brassicae. Species-specific primers and a TaqMan fluorogenic probe were designed to amplify a small region of P. brassicae ribosomal DNA. Total genomic DNA was extracted and purified from soil samples using commercial kits. The amount of pathogen DNA was quantified using a standard curve generated by including reactions containing different amounts of a plasmid carrying the P. brassicae target sequence. The PCR assay was optimized to give high amplification efficiency and three to four copies of the target DNA sequence were detected. Regression analysis showed that the standard curve was linear over at least six orders of magnitude (R2 > 0·99) and that the amplification efficiency was >92%. The detection limit in soil samples corresponded to 500 resting spores g−1 soil. The intersample reproducibility was similar to, or higher than, that of assays for other pathogens quantified in soil samples. Bait plants were used to validate the real-time PCR assay. The protocol developed was used to investigate the spatial distribution of P. brassicae DNA in different fields and a significant difference was found between in-field sampling points. The reproducibility of soil sampling was evaluated and showed no significant differences for samples with low levels of inoculum, whereas at higher levels differences occurred. Indicator kriging was used for mapping the probability of detecting P. brassicae within a 2-ha area of a field. A threshold level of 5 fg plasmid DNA g−1 soil, corresponding to approximately 3 × 103P. brassicae resting spores g−1 soil, is suggested for growing resistant cultivars. The results provide a robust and reliable technique for predicting the risk of disease development and for assessing the distribution of disease within fields.

 

A.-C. Wallenhammar, C. Almquist, M. Söderström, A. Jonsson

Plant Pathology
Volume 61, Issue 1, pages 16–28, February 2012

http://dx.doi.org/10.1111/j.1365-3059.2011.02477.x


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