Playing by the rules: Combinatorial domain swaps among “deconstructed” non-reducing polyketide synthases (NR-PKSs) revealed the rules behind product assembly (see scheme). The control exerted by individual catalytic domains was found to be sufficiently great that heterocombinations of domains from different NR-PKSs synthesized products in a predictable manner.
Dr. Anna L. Vagstad1,Adam G. Newman1,Philip A. Storm1,Katherine Belecki1,Prof. Jason M. Crawford2,Prof. Craig A. Townsend1,*
Angewandte Chemie International Edition