wet infiltrators in the dry lab
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wet infiltrators in the dry lab
Tooling the wet lab scientist with pseudo-dry lab skills. Towards a new breed of Data Scientist.
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CSHL Using CRISPR, biologists find a way to comprehensively identify anti-cancer drug targets | News & Features

CSHL Using CRISPR, biologists find a way to comprehensively identify anti-cancer drug targets | News & Features | wet infiltrators in the dry lab | Scoop.it
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White House hits pause on editing human germline cells

White House hits pause on editing human germline cells | wet infiltrators in the dry lab | Scoop.it
The White House has hit pause, for now, on clinical experiments that could alter the human germ line.
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Designer's toolkit for dynamic DNA nanomachines

Designer's toolkit for dynamic DNA nanomachines | wet infiltrators in the dry lab | Scoop.it
The latest DNA nanodevices created at the Technische Universitaet Muenchen (TUM)—including a robot with movable arms, a book that opens and closes, a switchable gear, and an actuator—may be intriguing in their own right, but that's not the point. They demonstrate a breakthrough in the science of ...

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Gut microbiome and anticancer immune response: really hot Sh*t!

Cell death and differentiation is a monthly research journal focused on the exciting field of programmed cell death and apoptosis. It provides a single accessible source of information for both scientists and clinicians, keeping them up-to-date with advances in the field. It encompasses programmed cell death, cell death induced by toxic agents, differentiation and the interrelation of these with cell proliferation.

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Microbiome dictating response to chemotherapy.. Wow.

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Ed Rybicki's curator insight, May 20, 2014 7:10 AM

Their title, not mine...B-)

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Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids | wet infiltrators in the dry lab | Scoop.it

Abstract

 

Human colorectal tumors bear recurrent mutations in genes encoding proteins operative in the WNT, MAPK, TGF-β, TP53 and PI3K pathways1, 2. Although these pathways influence intestinal stem cell niche signaling3, 4, 5, the extent to which mutations in these pathways contribute to human colorectal carcinogenesis remains unclear. Here we use the CRISPR-Cas9 genome-editing system6, 7 to introduce multiple such mutations into organoids derived from normal human intestinal epithelium. By modulating the culture conditions to mimic that of the intestinal niche, we selected isogenic organoids harboring mutations in the tumor suppressor genes APC, SMAD4 andTP53, and in the oncogenes KRAS and/or PIK3CA. Organoids engineered to express all five mutations grew independently of niche factors in vitro, and they formed tumors after implantation under the kidney subcapsule in mice. Although they formed micrometastases containing dormant tumor-initiating cells after injection into the spleen of mice, they failed to colonize in the liver. In contrast, engineered organoids derived from chromosome-instable human adenomas formed macrometastatic colonies. These results suggest that 'driver' pathway mutations enable stem cell maintenance in the hostile tumor microenvironment, but that additional molecular lesions are required for invasive behavior.


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Krishan Maggon 's curator insight, March 2, 2015 2:46 AM

NATURE MEDICINE | LETTER

 Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoidsMami Matano,Shoichi Date,Mariko Shimokawa,Ai Takano,Masayuki Fujii,Yuki Ohta,Toshiaki Watanabe,Takanori Kanai& Toshiro SatoAffiliationsContributionsCorresponding authorNature Medicine (2015) doi:10.1038/nm.3802Received 27 June 2014 Accepted 14 January 2015 Published online 23 February 2015

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Feng Zhang Development and Applications of CRISPR Cas9 for Genome Editing - YouTube

Watch on LabRoots at http://labroots.com/user/webinars/details/id/332 The Cas9 endonuclease from the microbial adaptive immune system CRISPR can be easily pr...
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Improving genome editing with drugs

Improving genome editing with drugs | wet infiltrators in the dry lab | Scoop.it
Scientists have discovered a way to enhance the efficiency of CRISPR genome editing with the introduction of a few key chemical compounds. This has important potential implications for correcting disease-causing genetic mutations.
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Genome Editing Comes of Age

Gene Targeting Techniques – an overview Genome Editing Tools rAAV CRISPR/Cas9 Key Considerations for Gene Editing Genome Editing at scale High through Knock-ou…

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Animated GIFs of Earth Over Time

Animated GIFs of Earth Over Time (7 photos)
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very nice google earth time lapses

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Stem cell casino | University of Cambridge

Stem cell casino | University of Cambridge | wet infiltrators in the dry lab | Scoop.it
Physicist Professor Ben Simons, newly appointed Herchel Smith Professor of Physics of Medicine, has spent the past two decades working with ultracold atomic gases and quantum chaos.
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when disciplines collide.. magic happens.

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Artificial Intelligence Raises New Hope for Cancer Patients

Artificial Intelligence Raises New Hope for Cancer Patients | wet infiltrators in the dry lab | Scoop.it
Can big data and smart algorithms generate real medical breakthroughs?
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By digitising biology, we open up the doors for A.I. and machine learning to have a massive impact on personalised health care

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New technology lets scientists easily rewrite living organisms' genetic code

New technology lets scientists easily rewrite living organisms' genetic code | wet infiltrators in the dry lab | Scoop.it
With a few easy tweaks, scientists can cut-and-paste DNA inside living cells, thanks to a promising new technique that could make possible everything from testing new drugs or curing genetic diseases. And researchers just discovered a way to make...
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Cheap genome-wide libraries for all - CRISPR EATER

 

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CRISPR “Kill Switches” for GMOs | The future?

Researchers create an inducible method to remove specific genes and even kill escaped genetically modified organisms.
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GEN | News Highlights:Bacteria use CRISPR to Recognize Themselves

GEN | News Highlights:Bacteria use CRISPR to Recognize Themselves | wet infiltrators in the dry lab | Scoop.it

Researchers at the Weizmann Institute of Science and Tel Aviv University have revealed how the bacterial immune system is to detect the difference between "foreign" and "self."

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Horizon Discovery: The Impact of Horizon’s Technology Platform - YouTube

Horizon’s unique gene editing platform powers everything we do. Learn more about how it works, and why the models that are generated are so powerful, changin...

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Scientists sound alarm over DNA editing of human embryos

Scientists sound alarm over DNA editing of human embryos | wet infiltrators in the dry lab | Scoop.it
Experts call for halt in research to work out safety and ethics issues.
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Mapping the precision of genome editing - Nature Biotech.

Mapping the precision of genome editing - Nature Biotech. | wet infiltrators in the dry lab | Scoop.it

Gabriel et al, 2015

Precise genome engineering in live cells at any locus promises to facilitate basic research and to enable personalized medicine. In particular, the recent development of the CRISPR-Cas9 system into a versatile and easy-to-use editing tool1 has been celebrated as a scientific breakthrough in the field. As genome engineering is adapted to clinical applications, a high level of precision—especially the avoidance of editing at sites other than the intended target—will be indispensable. In this issue, three very timely studies2, 3, 4 report methods for identifying off-target double-strand breaks produced by CRISPR-associated (Cas)9 nucleases and another class of programmable nucleases known as transcription activator–like effector nucleases (TALENs). Wang et al.2 and Tsai et al.3 rely on integration of a foreign DNA bait sequence into off-target double-strand breaks, whereas Frock et al.4 detect translocations of endogenous genomic sequences to the intended cleavage site (Fig. 1). All three methods attain an unprecedented level of comprehensiveness and sensitivity in off-target detection. The papers also highlight that the specificity of nucleases varies widely and must be evaluated case by case.


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Fesquet didier's curator insight, February 11, 2015 5:17 AM

encore le debat du OT...!

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Unlocking the key to immunological memory in bacteria

Unlocking the key to immunological memory in bacteria | wet infiltrators in the dry lab | Scoop.it
A powerful genome editing tool may soon become even more powerful. Researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) have unlocked the key to how bacteria are able to 'steal' genetic information from viruses and other foreign invaders for use in their own immunological memory ...
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Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast | wet infiltrators in the dry lab | Scoop.it
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Nice merging of technologies to create a library of human genes with their associated regulatory elements.

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Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice: Cell Reports

Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice: Cell Reports | wet infiltrators in the dry lab | Scoop.it
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Efficient generation of structural variants in mouse ES cells. Interestingly, size of structural variant doesn't affect CRISPR efficiency.

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City of Hope Team Demonstrates Strategy to Measure CRISPR, TALEN Off-target Effects

City of Hope Team Demonstrates Strategy to Measure CRISPR, TALEN Off-target Effects | wet infiltrators in the dry lab | Scoop.it
The approach was originally developed by another group for use with zinc finger nucleases.
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Researchers Create Walking, Muscle-Powered Biobots | TechCrunch

Researchers Create Walking, Muscle-Powered Biobots  | TechCrunch | wet infiltrators in the dry lab | Scoop.it
In today's mildly squiggy but kind of cool news, we learn that researchers at the University of Illinois at Urbana-Champaign have created tiny robots that..

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It's beginning...

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Google Ventures' Bill Maris on Moving Medicine Out of the Dark Ages

Google Ventures' Bill Maris on Moving Medicine Out of the Dark Ages | wet infiltrators in the dry lab | Scoop.it
Google Ventures is on the hunt for companies using computer science to improve health.
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"Clinicians treating patients based on “if you present with these symptoms, I’m going to treat you based on the knowledge in my head?” Those days are either disappearing or will soon disappear"

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