Genetic markers and maps are instrumental in quantitative trait locus (QTL) mapping in segregating populations. The resolution of QTL localization depends on the number of informative recombinations in the population and how well they are tagged by markers. Larger populations and denser marker maps are better for detecting and locating QTLs. Marker maps that are initially too sparse can be saturated or derived de novo from high-throughput omics data, (e.g. gene expression, protein or metabolite abundance). If these molecular phenotypes are affected by genetic variation due to a major QTL they will show a clear multimodal distribution. Using this information, phenotypes can be converted into genetic markers.
You will be an outstanding early career scientist with a PhD in a relevant subject area (awarded within the past eight years) and, ideally, some postdoctoral experience. You will have a proven aptitude for delivering excellent science publications and demonstrated potential to raise science income. You will be an outstanding and enthusiastic communicator who is ready to engage with students, peers and the general public.
Delivering improved varieties and crop protection through high quality seed is a vital component of modern agricultural production systems. It is critical that seed producers have an understanding of the biological factors that contribute to the development and maintenance of seed health, vigor and viability, and how those qualities are measured. This course presents the scientific background for production, handling, storage and quality control procedures in the seed industry. It updates participants on new information in these topics, including seed pathology and seed enhancement. The course targets professionals in the seed industry, new employees, consultants, and seed producers to extend and update their knowledge.
In 2015 the Seed Longevity Workshop of the International Society for Seed Science (ISSS) will take place in Wernigerode, Germany.
The topic of the conference is:
Seeds for future generations - Determinants of longevity
The workshop will bring together scientists involved in seed science and seed banking.
Session I: Seed banking - state of the artSession II: Role of pre- and post-harvest environmental factors on seed longevitySession III: Genetics of inter- and intra-specific variation of seed survivalSession IV: Physiology and biochemistry behind seed ageing - deleterious effects vs. repair mechanisms
In November 2014 the second call of the ZonMw Enabling Technologies programme will be opened. If you are interested in doing high-througput germination assays, you can write a project of max 30 k€. Also many other technologies will be available within this programme.
For those that missed the PhD defence of Hanzie He, but would still be interested to have a look (or for those who were present, but want to have another look): you can find the recordings of this event via this link
During re-establishment of desiccation tolerance (DT), early events promote initial protection and growth arrest, while late events promote stress adaptation and contribute to survival in the dry state.
Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose desiccation tolerance (DT) while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with abscisic acid (ABA). To gain temporal resolution and identify relevant genes in this process, data from a time series of microarrays were used to build a gene co-expression network. The network has two regions, namely early response (ER) and late response (LR). Genes in the ER region are related to biological processes, such as dormancy, acquisition of DT and drought, amplification of signals, growth arrest and induction of protection mechanisms (such as LEA proteins). Genes in the LR region lead to inhibition of photosynthesis and primary metabolism, promote adaptation to stress conditions and contribute to seed longevity. Phenotyping of 12 hubs in relation to re-establishment of DT with T-DNA insertion lines indicated a significant increase in the ability to re-establish DT compared with the wild-type in the lines cbsx4, at3g53040 and at4g25580, suggesting the operation of redundant and compensatory mechanisms. Moreover, we show that re-establishment of DT by polyethylene glycol and ABA occurs through partially overlapping mechanisms. Our data confirm that co-expression network analysis is a valid approach to examine data from time series of transcriptome analysis, as it provides promising insights into biologically relevant relations that help to generate new information about the roles of certain genes for DT.
DTL and ZonMw/NWO announce that a second call of the ZonMw programme Enabling Technologies Hotels is now open until January 15, 2015. This programme aspires to give researchers in public-private partnerships (the ‘guests’) access to high-end infrastructures within public research organisations…
Wilco Ligterink's insight:
If you are interested in doing high-througput germination assays, you can write a project for max 30 k€. Also many other technologies are available within this programme
On the 7th of October 2014 the Third Dutch Seed Symposium will be held in Wageningen. The agenda for this day is promising. If you really want to know what’s going on in seed research, or if you want to meet other people who work on the same subjects as you do: come and visit the 3rd Dutch Seed Symposium!
The Second Dutch Seed Symposium, in 2013, has been visited by more than 75 professionals in seed business and seed research.
Participation is open for members and non members of Plantum, but you have to register yourself before 26th of September. For participating an entrance fee of €100,- p.p., including lunch and drinks afterwards, will be charged with a maximum of €500,- per company for 5 or more participants of one company
Physiological and biochemical responses of Ricinus communis seedlings to different temperatures: a metabolomics approach
Paulo Roberto Ribeiro, Luzimar Gonzaga Fernandez, Renato Delmondez de Castro, Wilco Ligterink and Henk W M Hilhorst.
Compared with major crops, growth and development of Ricinus communis is still poorly understood. A better understanding of the biochemical and physiological aspects of germination and seedling growth is crucial for the breeding of high yielding varieties adapted to various growing environments. In this context, we analysed the effect of temperature on growth of young R. communis seedlings and we measured primary and secondary metabolites in roots and cotyledons. Three genotypes, recommended to small family farms as cash crop, were used in this study.
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