Virology and Bioi...
Follow
Find tag "miRNA"
76.4K views | +2 today
Virology and Bioinformatics from Virology.ca
Virus and bioinformatics articles with some microbiology and immunology thrown in for good measure
Your new post is loading...
Your new post is loading...
Scooped by Chris Upton + helpers
Scoop.it!

A Baculovirus-Encoded MicroRNA (miRNA) Suppresses Its Host miRNA Biogenesis by Regulating the Exportin-5 Cofactor Ran

MicroRNAs have emerged as key players in the regulation of various biological processes in eukaryotes, including host-pathogen interactions. Recent studies suggest that viruses encode miRNAs to manipulate their host gene expression to ensure their effective proliferation, whereas the host limits virus infection by differentially expressing miRNAs that target essential viral genes.

more...
No comment yet.
Scooped by Jeff Habig
Scoop.it!

Induction of the Cellular MicroRNA, Hs_154, by West Nile Virus Contributes to Virus-Mediated Apoptosis through Repression of Antiapoptotic Factors

Induction of the Cellular MicroRNA, Hs_154, by West Nile Virus Contributes to Virus-Mediated Apoptosis through Repression of Antiapoptotic Factors | Virology and Bioinformatics from Virology.ca | Scoop.it

Examination of cellular miRNA profiles in West Nile virus (WNV)-infected HEK293 and SK-N-MC cells revealed increased expression of multiple miRNA species. One of these miRNAs, Hs_154, was significantly induced not only in WNV-infected neuronal cells in culture but also in the central nervous system tissues of infected mice and, upon transfection, caused a significant reduction in viral replication.

more...
No comment yet.
Rescooped by Chris Upton + helpers from Virology News
Scoop.it!

Delivering Silence | The Scientist

Delivering Silence | The Scientist | Virology and Bioinformatics from Virology.ca | Scoop.it
Since the discovery of RNA interference almost 15 years ago, researchers have tried to devise effective ways to get these short gene-silencing transcripts inside cells. But techniques for in vivo delivery have fallen short. For therapies that require long-term gene knockdown, DNA viruses that insert the microRNA (miRNA) genes into the target cell’s genome were the best bet. But that approach produced large numbers of miRNAs, which overloaded the miRNA-processing proteins in the nucleus and resulted in cellular toxicity. For short-term therapies, mature miRNAs encapsulated in liposomes or conjugated to cholesterol were used, but they have been difficult to target to a specific cell type.

Via Ed Rybicki
more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

miRviewer- http://people.csail.mit.edu/akiezun/miRviewer/

miRviewer presents a global view of homologous miRNA genes in many species.

more...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Virology Journal | Abstract | Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct

Background
RNA interference (RNAi) has been used as a promising approach to inhibit human immunodeficiency virus type 1 (HIV-1) replication for both in vitro and in vivo animal models. However, HIV-1 escape mutants after RNAi treatment have been reported. Expressing multiple small interfering RNAs (siRNAs) against conserved viral sequences can serve as a genetic barrier for viral escape, and optimization of the efficiency of this process was the aim of this study.

Results
An artificial polycistronic transcript driven by a CMV promoter was designed to inhibit HIV- 1 replication. The artificial polycistronic transcript contained two pre-miR-30a backbones and one pre-miR-155 backbone, which are linked by a sequence derived from antisense RNA sequence targeting the HIV-1 env gene. Our results demonstrated that this artificial polycistronic transcript simultaneously expresses three anti-HIV siRNAs and efficiently inhibits HIV-1 replication. In addition, the biosafety of MT-4 cells expressing this polycistronic miRNA transcript was evaluated, and no apparent impacts on cell proliferation rate, interferon response, and interruption of native miRNA processing were observed.

Conclusions
The strategy described here to generate an artificial polycistronic transcript to inhibit viral replication provided an opportunity to select and optimize many factors to yield highly efficient constructs expressing multiple siRNAs against viral infection.

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

Defining and providing robust controls for microRNA prediction

Motivation: microRNAs are short non-coding RNAs that regulate gene expression by inhibiting target mRNA genes. Next-generation sequencing combined with bioinformatics analyses provide an opportunity to predict numerous novel miRNAs. The efficiency of these predictions relies on the set of positive and negative controls used.

 

Bioinformatics paper

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

MicrobiologyBytes » First RNA virus-encoded miRNAs

MicrobiologyBytes » First RNA virus-encoded miRNAs | Virology and Bioinformatics from Virology.ca | Scoop.it

Although >250 viral microRNAs (miRNAs) are expressed by a range of nuclear DNA viruses, efforts to identify miRNAs expressed by RNA viruses have so far been in vain. In PNAS, Kincaid et al. report the identification of five miRNAs encoded by the delta retrovirus bovine leukemia virus (BLV) that are expressed in BLV-transformed B cells. It appears likely that these viral miRNAs play an important role in BLV pathogenesis:

 

more...
No comment yet.