Viruses and Bioinformatics from Virology.uvic.ca
94.2K views | +1 today
Follow
Viruses and Bioinformatics from Virology.uvic.ca
Virus and bioinformatics articles with some microbiology and immunology thrown in for good measure
Your new post is loading...
Your new post is loading...
Scooped by Cindy
Scoop.it!

Humanized mouse models to study human cell-mediated and humoral responses to dengue virus

Several candidate dengue virus vaccines are in clinical trials and show promise as an effective measure to control dengue. However, it is becoming clear that additional vaccine candidates may be needed as there is concern about the durability of the immune response to all four serotypes of vaccine components and efficacy varies dependent on the immune status of the individual. The lack of an appropriate animal model to mimic human dengue has deterred the development of vaccines and anti-viral therapies to dengue virus. The focus of this review is to discuss advances in the development of humanized animal models and to highlight how they could be used for antiviral and dengue vaccine testing if limitations with cell-mediated immunity and seroconversion to IgG are overcome.
more...
No comment yet.
Scooped by Cindy
Scoop.it!

Inhibition of protein kinase C promotes dengue virus replication

Inhibition of protein kinase C promotes dengue virus replication | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Our in silico analyses indicated that the non-structural protein 5 (NS5), especially the RNA-dependent RNA polymerase (RdRp) domain, contains conserved phosphorylation sites for protein kinase C (PKC). Phosphorylation of NS5 RdRp was further verified by PKC in vitro kinase assay. Inhibitions of PKC by a PKC-specific chemical inhibitor or siRNA suppressed NS5 phosphorylation in vivo, increased viral replication and reduced viability of the DENV-infected cells. In contrary, activation of PKC effectively suppressed intracellular viral number.
more...
No comment yet.
Rescooped by Kenzibit from Host Cell & Pathogen Interactions
Scoop.it!

Effective suppression of Dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-termina...

Approximately 100 million confirmed infections and 20,000 deaths are caused by Dengue virus (DENV) outbreaks annually. Global warming and rapid dispersal have resulted in DENV epidemics in formally non-endemic regions. Currently no consistently effective preventive measures for DENV exist, prompting development of transgenic and paratransgenic vector control approaches. Production of transgenic mosquitoes refractory for virus infection and/or transmission is contingent upon defining antiviral genes that have low probability for allowing escape mutations, and are equally effective against multiple serotypes. Previously we demonstrated the effectiveness of an anti-viral group I intron targeting U143 of the DENV genome in mediating trans-splicing and expression of a marker gene with the capsid coding domain. In this report we examine the effectiveness of coupling expression of DeltaN Bax to trans-splicing U143 intron activity as a means of suppressing DENV infection of mosquito cells.
more...
No comment yet.
Scooped by Hannah Davis
Scoop.it!

Brazil 2014 dengue risk predicted

Brazil 2014 dengue risk predicted | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it

"Scientists have developed an "early warning system" to alert authorities to the risk of dengue fever outbreaks in Brazil during the World Cup.

The analysis, published in The Lancet Infectious Diseases, estimates the chances of an outbreaks of the mosquito-borne infection disease."

more...
No comment yet.
Scooped by Kenzibit
Scoop.it!

A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches

Recent epidemics of dengue viruses (DENV) coupled with new outbreaks on the horizon have renewed the demand for novel detection methods that have the ability to identify this viral pathogen prior to the manifestation of symptoms.
more...
No comment yet.
Rescooped by Kenzibit from Host Cell & Pathogen Interactions
Scoop.it!

Dengue Viral Fusion

Dengue was created for Irene Bosch, Ph.D. at University of Massachusetts and Yorgo Modis, Ph.D. from Yale University. This animation was an educational proje...
more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

The pathogenesis of dengue

The pathogenesis of dengue | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Better understanding of the underlying mechanisms leading to severe dengue is crucial if we are to develop better treatments.
Chris Upton + helpers's insight:

From MicrobiologyBytes

more...
No comment yet.
Scooped by Rebecca Kreston
Scoop.it!

Fears downpour may fuel dengue cases - ABC News (Australian Broadcasting Corporation)

Fears downpour may fuel dengue cases - ABC News (Australian Broadcasting Corporation) | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Health experts say days of rain in Cairns, in far north Queensland, could lead to more people catching dengue fever.
more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

Dengue Affects Genes, Function of Mosquito Salivary Glands

Mosquitoes infected with dengue virus experience an array of changes in the activity of genes and associated functions of their salivary glands, and these changes may lead to increased virus transmission.

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

Discovery and Optimization of 2,4-Diaminoquinazol... new dengue drug

The results of a high-throughput screening assay using the DENV-2 replicon showed that the 2,4-diaminoquinazoline derivative 4a has a high dengue virus inhibitory activity (EC(50) = 0.15 μM).

more...
No comment yet.
Scooped by Cindy
Scoop.it!

Viral pathogenesis: Taking a bite : Nature Reviews Microbiology

Aedes spp. mosquitoes are important vectors of disease as they inject pathogen-containing saliva into a vertebrate host.
more...
No comment yet.
Scooped by Cindy
Scoop.it!

Interrupting the transmission cycle: A protein required for dengue virus infection of mosquitoes

Interrupting the transmission cycle: A protein required for dengue virus infection of mosquitoes | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
A study published on October 22nd in PLOS Pathogens introduces a candidate target for a transmission-blocking vaccine that interferes with virus infection of the mosquito after it feeds on the blood of infected hosts (such a vaccine would be a valuable complement to traditional DENV vaccines in development that seek to prevent human infection).
more...
No comment yet.
Rescooped by burkesquires from Dengue and other Quasispecies-Like viruses
Scoop.it!

A global compendium of human dengue virus occurrence

A global compendium of human dengue virus occurrence | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Scientific Data, Published online: 27 May 2014; | doi:10.1038/sdata.2014.4

Via Mel Melendrez-Vallard
more...
No comment yet.
Scooped by Hannah Davis
Scoop.it!

Disease threat to World Cup fans

Disease threat to World Cup fans | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it

"Fans heading to Brazil for the World Cup may need to guard against dengue fever - a disease carried by mosquitoes for which there is no vaccine."

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

Dengue—an infectious disease of staggering proportions : The Lancet

About 50—100 million dengue infections occur every year, and the burden of disease not only falls heavily on southeast Asia, but also throughout Latin America. Today, about two-fifths of the world's population live in dengue-endemic countries. 

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

H5N1: US: Dengue back in the Florida Keys

H5N1: US: Dengue back in the Florida Keys | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Via the Shots blog at NPR.org: Dengue Fever No Longer Just A Visitor To Florida Keys. If you're heading down to the Florida Keys for spring break, pack bug spray and long-sleeve shirts. After a 60-year hiatus, the mosquito-borne illness...
more...
No comment yet.
Scooped by Marion Koopmans
Scoop.it!

Bedeviled by Dengue - Scientist (blog)

Bedeviled by Dengue - Scientist (blog) | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it
Scientist (blog) Bedeviled by Dengue Scientist (blog) The cells enclose the viruses in a sac—a process called receptor-mediated endocytosis—that would normally digest the viral captives with enzymes in an acidic milieu, like a piece of food in the...
Chris Upton + helpers's insight:

Nice mini review, including the discussion on antibody-mediated effects on pathogenesis. I do miss the recent data on antibodies rendering immature particles infectious, work from University of Groningen scientist professor Jolande Smit

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

Achilles heel of dengue virus identified: Target for future vaccines

This study for the first time shows what dengue virus region the immune system of humans target when they are fighting off the virus.
more...
No comment yet.
Scooped by Jeff Habig
Scoop.it!

PLoS Neglected Tropical Diseases: Assessing the Potential of a Candidate Dengue Vaccine with Mathematical Modeling

PLoS Neglected Tropical Diseases: Assessing the Potential of a Candidate Dengue Vaccine with Mathematical Modeling | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it

To date, most models of dengue transmission have been limited in scope and focused on specific questions in transmission dynamics. However, many aspects of the dynamics of dengue transmission are still not fully understood. In order for models to be accurate, realistic, and useful, there is an urgent need for improved understanding of dengue virology and immunology, as well as the entomological, social, and environmental factors that modulate dengue transmission. As these facets of dengue biology are further investigated, we will gain confidence that future mathematical models may come close to an accurate representation of true dengue epidemics.

more...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

PLoS Neglected Tropical Diseases: Assessing the Potential of a Candidate Dengue Vaccine with Mathematical Modeling

PLoS Neglected Tropical Diseases: Assessing the Potential of a Candidate Dengue Vaccine with Mathematical Modeling | Viruses and Bioinformatics from Virology.uvic.ca | Scoop.it

To date, most models of dengue transmission have been limited in scope and focused on specific questions in transmission dynamics. However, many aspects of the dynamics of dengue transmission are still not fully understood. In order for models to be accurate, realistic, and useful, there is an urgent need for improved understanding of dengue virology and immunology, as well as the entomological, social, and environmental factors that modulate dengue transmission. As these facets of dengue biology are further investigated, we will gain confidence that future mathematical models may come close to an accurate representation of true dengue epidemics.

more...
No comment yet.