Virology and Bioi...
Follow
Find tag "H5N1"
67.8K views | +0 today
Virology and Bioinformatics from Virology.ca
Virus and bioinformatics articles with some microbiology and immunology thrown in for good measure
Your new post is loading...
Your new post is loading...
Scooped by burkesquires
Scoop.it!

Identification, Characterization, and Natural Selection of Mutations Driving Airborne Transmission of A/H5N1 Virus: Cell

Identification, Characterization, and Natural Selection of Mutations Driving Airborne Transmission of A/H5N1 Virus: Cell | Virology and Bioinformatics from Virology.ca | Scoop.it

Recently, A/H5N1 influenza viruses were shown to acquire airborne transmissibility between ferrets upon targeted mutagenesis and virus passage. The critical genetic changes in airborne A/Indonesia/5/05 were not yet identified. Here, five substitutions proved to be sufficient to determine this airborne transmission phenotype. Substitutions in PB1 and PB2 collectively caused enhanced transcription and virus replication. One substitution increased HA thermostability and lowered the pH of membrane fusion. Two substitutions independently changed HA binding preference from α2,3-linked to α2,6-linked sialic acid receptors. The loss of a glycosylation site in HA enhanced overall binding to receptors. The acquired substitutions emerged early during ferret passage as minor variants and became dominant rapidly. Identification of substitutions that are essential for airborne transmission of avian influenza viruses between ferrets and their associated phenotypes advances our fundamental understanding of virus transmission and will increase the value of future surveillance programs and public health risk assessments.

more...
No comment yet.
Scooped by burkesquires
Scoop.it!

HPAI H5N1 Clade Classification Tool and Search - Influenza Research Database

Searchable database of influenza genomes with visualization and analysis tools.
burkesquires's insight:

The November release of the IRD database includes the clade classification of HA/H5 sequences, based on our new 

Highly Pathogenic H5N1 Clade Classification Tool. 
Search for sequences by clade, or classify your own HA sequence.

 

Full Disclosure: I used to work with hte IRD.

more...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

The Multibasic Cleavage Site in H5N1 Virus Is Critical for Systemic Spread along the Olfactory and Hematogenous Routes in Ferrets

The Multibasic Cleavage Site in H5N1 Virus Is Critical for Systemic Spread along the Olfactory and Hematogenous Routes in Ferrets | Virology and Bioinformatics from Virology.ca | Scoop.it

Here we determined the virus distribution of HPAI H5N1 virus in ferrets in time and space—including along the olfactory route—and the role of the MBCS in systemic replication. Intranasal inoculation with wild-type H5N1 virus revealed extensive replication in the olfactory mucosa, from which it spread to the olfactory bulb and the rest of the CNS, including the cerebrospinal fluid (CSF). Virus spread to the heart, liver, pancreas, and colon was also detected, indicating hematogenous spread. Ferrets inoculated intranasally with H5N1 virus lacking an MBCS demonstrated respiratory tract infection only. In conclusion, HPAI H5N1 virus can spread systemically via two different routes, olfactory and hematogenous, in ferrets. This systemic spread was dependent on the presence of the MBCS in HA.

 

This is why we need sequences of aerosol transmitted viruses, people...!

 

Image by Russell Kightley Media

more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

H5N1: CIDRAP: New influenza A virus found in bats

Via CIDRAP, Lisa Schnirring writes: New influenza A virus found in bats. Click through for the whole report and a link to the original study. Excerpt: A research team today announced the discovery of a new influenza A virus in...
more...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

H5N1 bird flu infection may be more common, less deadly, than thought

H5N1 bird flu infection may be more common, less deadly, than thought | Virology and Bioinformatics from Virology.ca | Scoop.it
The World Health Organization says the H5N1 bird flu kills nearly 60% of people who become infected, but a study released Thursday suggests the true fatality rate may actually be much lower.

 

...meaning a lot more people have been infected.  Possibly meaning the next pandemic is close thn we thought.

more...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Easy Jump for H5N1 from Bird to Mammal

Easy Jump for H5N1 from Bird to Mammal | Virology and Bioinformatics from Virology.ca | Scoop.it
Hybrid viruses derived from an H5N1 bird flu strain can infect guinea pigs through the air.
more...
Ed Rybicki's curator insight, May 3, 2013 11:14 AM

It is rather concerning that these guys did NOT have to make HA mutations to get their viruses easily transmissible - they just to make reassortants with H5N1 and H1N1pdm viruses.  As could happen in pigs or poultry anywhere both viruses occur....

 
Scooped by Kenzibit
Scoop.it!

Avian influenza virus H5N1 induces rapid interferon-beta production but shows more potent inhibition to retinoic acid-inducible gene I expression than H1N1 in vitro

The mechanisms through which the avian influenza virus H5N1 modulate the host's innate immune defense during invasion, remains incompletely understood.

The mechanisms through which the avian influenza virus H5N1 modulate the host's innate immune defense during invasion, remains incompletely understood. RIG-I as a pattern recognition receptor plays an important role in mediating innate immune response induced by influenza virus.

more...
No comment yet.
Rescooped by Ed Rybicki from The Microbiology Mine
Scoop.it!

H5N1 Pandemic Information News: Map: Worldwide Confirmed ...

H5N1 Pandemic Information News: Map: Worldwide Confirmed ... | Virology and Bioinformatics from Virology.ca | Scoop.it
Map: Worldwide Confirmed Human Cases Updated. Click on image to enlarge. Click on title for postmark descriptions. Located on the right side-bar for future reference. Posted by Commonground at 6:44 AM ...

Via Elsevier Microbiol*
more...
No comment yet.
Scooped by Chris Upton + helpers
Scoop.it!

H5N1: Indonesia: "Dozens" with H5N1 symptoms in South Sulawesi village

Via Treyfish at FluTrackers, a computer-translated report: Indonesia - Gowa-Dozens of residents exhibit bird flu symptoms of fever, cough, short breath after hundreds of chickens die - South Sulawesi.
more...
No comment yet.