Fetchnotes, the Twitter-like note-taking app that employs @ usernames and # hashtags to keep your to-do lists organized, is releasing an updated version today that now connects the items you mention in your notes with their real-world counterparts.
Next-generation sequencing allows detection of minor variants in a heterogeneous sample. However, errors in PCR and sequencing pose limits on its sensitivity. A group at University of Washington developed a method, called Duplex Sequencing, to dramatically improve accuracy by sequencing both strands of each DNA duplex. Mutations that are detected in the consensus sequence of one strand but not the other are discounted as technical errors.
The authors adopted the method to Illumina sequencing. It involves the use of modified adaptors that have a tag with random sequence attached. After ligation of these modified adaptors, each duplex DNA fragment is flanked by two different tags and subjected to paired-end sequencing. Sequences of the same duplex from the complementary strands can therefore be uniquely identified by having the same tags on either ends. Comparing sequences of the two strands allows identification of true mutations. The authors estimated that Duplex sequencing has a theoretical background error rate of less than one per 10^9 nucleotides sequenced.
RT Billionaire space entrepreneur wants vegetarian-only colony on Mars RT A US billionaire and co-founder of PayPal, Elon Musk, has made plans to build a settlement for 80,000 people on Mars when technology makes it possible for man to live there –...
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