Uniparts - Biología Molecular
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Opciones Integrales para las Ciencias de la Vida
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Rescooped by Uniparts, SA de CV from Quantitative PCR for gene expression analyses
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Gene expression improves the definition of a breast cancer subtype

Gene expression improves the definition of a breast cancer subtype | Uniparts - Biología Molecular | Scoop.it

The study conducted by the Vall d’Hebron Institute of Oncology (VHIO) inconjunction with the GEICAM cooperative group and other American and Canadian researchers has led to a change in the definition of hormone-sensitive breast tumours.


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Rescooped by Uniparts, SA de CV from Synthetic Biology
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Streamlined engineering for synthetic biology

Streamlined engineering for synthetic biology | Uniparts - Biología Molecular | Scoop.it

Researchers are injecting ever more conscious design into their bioengineering workflow.


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Unraveling the Threads: Simplest cotton genome offers clues for fiber improvements

Unraveling the Threads: Simplest cotton genome offers clues for fiber improvements | Uniparts - Biología Molecular | Scoop.it

From the stockings decorating mantles to the new outfits in display windows calling to shoppers, cotton is woven into the fabric of the holiday season. For bioenergy researchers, however, fiber composition matters more than color and texture as each cotton strand is composed of more than two dozen coils of cellulose, a target biomass for next-generation biofuels.


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Laser microdissection, sequencing method combine to analyze whole genome

Laser microdissection, sequencing method combine to analyze whole genome | Uniparts - Biología Molecular | Scoop.it

By combining laser microdissection and next-generation sequencing (NGS), researchers at the Ruhr University Bochum (RUB) Department of General and Molecular Botany (Bochum, Germany) have analyzed gene activity in the whole genome of small, multicellular fungi in one pass. 


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Rescooped by Uniparts, SA de CV from TAL effector science
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A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes


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dromius's curator insight, December 18, 2012 4:35 PM

Thanks Pawel!

 

Schmid-Burgk et al, (2012) - Nature Biotech

 

Transcription activator–like (TAL) effector proteins derived from Xanthomonas species have emerged as versatile scaffolds for engineering DNA-binding proteins of user-defined specificity and functionality. Here we describe a rapid, simple, ligation-independent cloning (LIC) technique for synthesis of TAL effector genes. Our approach is based on a library of DNA constructs encoding individual TAL effector repeat unit combinations that can be processed to contain long, unique single-stranded DNA overhangs suitable for LIC. Assembly of TAL effector arrays requires only the combinatorial mixing of fluids and has exceptional fidelity. TAL effector nucleases (TALENs) produced by this method had high genome-editing activity at endogenous loci in HEK 293T cells (64% were active). To maximize throughput, we generated a comprehensive 5-mer TAL effector repeat unit fragment library that allows automated assembly of >600 TALEN genes in a single day. Given its simplicity, throughput and fidelity, LIC assembly will permit the generation of TAL effector gene libraries for large-scale functional genomics studies.

Rescooped by Uniparts, SA de CV from Quantitative PCR for gene expression analyses
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How to Design qPCR Primers Spanning Exon Junctions Using PrimerQuest

This tutorial demonstrates how to use PrimerQuest to design PCR or qPCR primers that span exon junctions for a gene. PrimerQuest is one of IDT's scientific d...

Via Integrated DNA Technologies
Uniparts, SA de CV's insight:

Conoce esta excelente herramienta que te permite diseñar ensayos para qPCR qe favorescan la amplificación de cDNA al diseñar secuencias en las uniones de los exones de tu gen de interés.

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Pesticide Use Increases as GMO Technology Backfires « Set You ...

Pesticide Use Increases as GMO Technology Backfires « Set You ... | Uniparts - Biología Molecular | Scoop.it
The GMO nightmare continues to unfurl, as the crop technology designed to reduce the need for pesticides has backfired. Farmers' heavy adoption of these modified crops has sparked an increase in “superweeds” and ...

Via Roberto Fernández Crespo
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Rescooped by Uniparts, SA de CV from DNA and RNA Research
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Protein strongest just before death

Protein strongest just before death | Uniparts - Biología Molecular | Scoop.it
Researchers at MSU have discovered a protein that does its best work with one foot in the grave.
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Rescooped by Uniparts, SA de CV from Industrial Microbiology
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Biologists engineer algae to make complex anti-cancer 'designer' drug

Biologists engineer algae to make complex anti-cancer 'designer' drug | Uniparts - Biología Molecular | Scoop.it
Biologists have succeeded in genetically engineering algae to produce a complex and expensive human therapeutic drug used to treat cancer.

Via Manuel Sánchez
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Upcoming Webinar: Crop Improvement for Human Nutrition Symposium | PBGworks

Upcoming Webinar: Crop Improvement for Human Nutrition Symposium | PBGworks | Uniparts - Biología Molecular | Scoop.it

Friday, December 14, 2012 (9:15-11:45 ET & 1:30-3:30 ET)

 

Register now to join the Plant Breeding and Genomics Community of Practice for a live two-part web broadcast of Michigan State University’s Crop Improvement for Human Nutrition Symposium.

 

Webinar 1: Morning Session 9:15 am – 10:15 ET

Richard Sayre

Los Alamos National Laboratory

Cassava for Africa

 

10:45-11:45 am ET

Dilrukshi Thavarajah North Dakota State University

Pulse Biofortification: Linking Food Systems for Better Human Heath

 

Register for morning session now at: https://www1.gotomeeting.com/register/312159465

 

Webinar 2: Afternoon Session

1:30-2:30 pm ET

Torbert Rocheford

Purdue University

Genetic, Genomic, and Human Visual Analyses and Selection Approaches for Orange, High Provitamin A Maize Grain for Africa

 

2:30-3:30 pm ET

Irwin Goldman University of Wisconsin

Take Two Onions and Call Me in the Morning: Possibilities and Pitfalls in Breeding for Health-Related Traits

Register for afternoon session now at:

https://www1.gotomeeting.com/register/167134233

 


Via Valerio Hoyos-Villegas
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Rescooped by Uniparts, SA de CV from Next Generation Sequencing (NGS)
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Improving NGS Target Capture

Target capture methods allow for enrichment of selected regions of interest from DNA samples prior to next generation sequencing (NGS). By selecting target regions, or expression subsets, investigators can maximize both the number of samples that they can analyze and the number of times individual sequences in a sample are replicated (coverage) for every NGS run. This results in the highest statistical power per dollar spent. This webinar will discuss target capture methods and how IDT xGen™ target capture probes can be used to improve your sequencing data.


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Scientists Pair Blood Test and Gene Sequencing to Detect Cancer

Scientists Pair Blood Test and Gene Sequencing to Detect Cancer | Uniparts - Biología Molecular | Scoop.it
Scientists at the Johns Hopkins Kimmel Cancer Center have combined the ability to detect cancer DNA in the blood with genome sequencing technology in a test that could be used to screen for cancers, monitor cancer patients for recurrence and find...

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The bench scientist's guide to statistical analysis of RNA-Seq data

RNA sequencing (RNA-Seq) is emerging as a highly accurate method to quantify transcript abundance. However, analyses of the large data sets obtained by sequencing the entire transcriptome of organisms have generally been performed by bioinformatics specialists.


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Rescooped by Uniparts, SA de CV from DNA and RNA Research
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DNA: making counterfeiting, theft much more difficult

DNA: making counterfeiting, theft much more difficult | Uniparts - Biología Molecular | Scoop.it
Applied DNA Science has partnered with the UK Metropolitan Police to offer DNA-based property marking kits for to residents who reside in areas with h

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Rescooped by Uniparts, SA de CV from Amazing Science
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Algae can be a big cost-cutter in making drugs

Algae can be a big cost-cutter in making drugs | Uniparts - Biología Molecular | Scoop.it

Expensive biotech drugs now made in high-tech manufacturing plants can be grown much more cheaply in genetically engineered algae, according to a paper published Monday in the prestigious Proceedings of the National Academy of Sciences.

 

Manufacturing costs can be reduced by 90 percent for these drugs, translating into half off their sales price, said Stephen Mayfield, a UC San Diego professor and senior author of the paper. The savings would provide financial relief to patients, health insurers and the federal government -- and the technology could boost San Diego's growing algae biotech sector.

 

The technology could make obsolete the manufacturing plants that grow specialized mammal cells in carefully monitored and chemically controlled vats, plants that cost hundreds of millions to build.

In their place would stand greenhouses containing transparent plastic bags filled with algae, water and diluted fertilizer. In Mayfield’s vision, scientists will design drugs on a computer, get the appropriate DNA by mail order from a manufacturer, then slip the DNA into the algae of choice. Ramping up production would be simply a matter of adding more bags.


Via Dr. Stefan Gruenwald
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Alan Yoshioka's curator insight, December 28, 2012 5:28 PM

Algae photobioreactors can easily be planned into vertical farms to provide some additional high end cash flows to boost economics of the facilities ...

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Using Genetic Engineering to Create a Chair

Using Genetic Engineering to Create a Chair | Uniparts - Biología Molecular | Scoop.it
Assay Depot sponsored NYU Gallatin's IGEM team, which attempted to use genetic engineering to create a GMO chair.

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Scientists Develop Technique to Help Prevent Inherited Disorders in Humans

A joint team of scientists from The New York Stem Cell Foundation(NYSCF) Laboratory and Columbia University Medical Center (CUMC) has developed a technique that may prevent the inheritance of mitochondrial diseases in children. The study is published online today in Nature. 


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Rescooped by Uniparts, SA de CV from DNA and RNA Research
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What mechanism generates our fingers and toes?

What mechanism generates our fingers and toes? | Uniparts - Biología Molecular | Scoop.it
Genetic studies at the IRCM confirm a mathematical model
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Science: The Tale of the TALEs (2012)

Science: The Tale of the TALEs (2012) | Uniparts - Biología Molecular | Scoop.it
Biologists have turned plant pest proteins into tools for studying and reshaping genomes of many species.

Some of biology's best technologies come from unexpected places. The green fluorescent protein that lit up biology with its ability to track proteins and gene expression in cells was borrowed from a jellyfish. A heat-stable enzyme from a bacterium often found in hot springs made the polymerase chain reaction method practical, facilitating the easy copying of DNA fragments needed for a myriad of applications, including the DNA fingerprinting used so widely to identify people. Now, thanks in part to inspiration that struck during a lunchtime discussion, proteins from a feared plant pest are poised to make genome engineering, the large-scale, directed manipulation of genes, routine for researchers studying a variety of organisms, including yeast and humans.
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Biswapriya Biswavas Misra's curator insight, December 16, 2012 3:49 AM

Biologists have turned plant pest proteins into tools for studying and reshaping genomes of many species.

Some of biology's best technologies come from unexpected places. The green fluorescent protein that lit up biology with its ability to track proteins and gene expression in cells was borrowed from a jellyfish. A heat-stable enzyme from a bacterium often found in hot springs made the polymerase chain reaction method practical, facilitating the easy copying of DNA fragments needed for a myriad of applications, including the DNA fingerprinting used so widely to identify people. Now, thanks in part to inspiration that struck during a lunchtime discussion, proteins from a feared plant pest are poised to make genome engineering, the large-scale, directed manipulation of genes, routine for researchers studying a variety of organisms, including yeast and humans.

Rescooped by Uniparts, SA de CV from Virology and Bioinformatics from Virology.ca
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Identification of novel influenza A virus proteins translated from PA mRNA


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burkesquires's curator insight, December 13, 2012 1:51 PM

"Many replication events are involved in the influenza A virus life cycle, which are accomplished by different virus proteins with specific functions. However, because the size of influenza virus genome is limited, the virus uses different mechanisms to express multiple viral proteins from a single gene segment. The M2 and NS2 proteins are produced by splicing and several novel influenza A virus proteins, such as PB1-F2, PB1-N40, and PA-X have recently been identified. Here, we identified novel PA-related proteins in influenza A virus-infected cells. These newly identified proteins are translated from the eleventh and thirteenth in-frame AUG codons in the PA mRNA and are, therefore, N-terminally truncated forms of PA, which we named PA-N155 and PA-N182, respectively. The eleventh and thirteenth AUG codons are highly conserved among influenza A viruses, and the PA-N155 and PA-N182 proteins were detected in cells infected with various influenza A viruses isolated from different host species, suggesting the expression of these N-truncated PAs is universal in nature among influenza A viruses. These N-truncated PAs did not show polymerase activity when expressed together with PB1 and PB2; however, mutant viruses lacking the N-truncated PAs replicated more slowly in cell culture and had lower pathogenicity in mice than did wild-type virus. These results suggest that these novel PA-related proteins likely possess important functions in the replication cycle of influenza A virus.

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Rescooped by Uniparts, SA de CV from DNA and RNA Research
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Reconstruction of prehistoric DNA refutes criticism on theory of evolution

Reconstruction of prehistoric DNA refutes criticism on theory of evolution | Uniparts - Biología Molecular | Scoop.it
Scientists from VIB, KU Leuven, UGent and Harvard have succeeded in reconstructing DNA and proteins from prehistoric yeast cells. This made it possible to determine how genes developed and evolved into their current form over more than 100 million years.
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Rescooped by Uniparts, SA de CV from Amazing Science
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From fish to human: Research reveals how fins became legs

From fish to human: Research reveals how fins became legs | Uniparts - Biología Molecular | Scoop.it
Vertebrates' transition to living on land, instead of only in water, represented a major event in the history of life. Now, researchers reporting in the December issue of the Cell Press journal Developmental Cell provide new evidence that the development of hands and feet occurred through the gain of new DNA elements that activate particular genes.

In order to understand how fins may have evolved into limbs, researchers led by Dr. Gómez-Skarmeta and his colleague Dr. Fernando Casares at the same institute introduced extra Hoxd13, a gene known to play a role in distinguishing body parts, at the tip of a zebrafish embryo's fin. Surprisingly, this led to the generation of new cartilage tissue and the reduction of fin tissue -- changes that strikingly recapitulate key aspects of land-animal limb development. The researchers wondered whether novel Hoxd13 control elements may have increased Hoxd13 gene expression in the past to cause similar effects during limb evolution. They turned to a DNA control element that is known to regulate the activation of Hoxd13 in mouse embryonic limbs and that is absent in fish.

"We found that in the zebrafish, the mouse Hoxd13 control element was capable of driving gene expression in the distal fin rudiment. This result indicates that molecular machinery capable of activating this control element was also present in the last common ancestor of finned and legged animals and is proven by its remnants in zebrafish," says Dr. Casares.

The picture above depicts a zebrafish embryo which developed a limb that looks more like a leg than a fin, after being engineered to produce the HoxD13 protein within the cells at the tip of the developing fin. Changes in HoxD13 production likely contributed to the transition from fin to leg development, during animal evolution.
Via Dr. Stefan Gruenwald
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“Avances en la evaluación de los nuevos marcadores para la detección de las lesiones precursoras y el cáncer cérvico uterino”

“Avances en la evaluación de  los nuevos marcadores para  la detección de las lesiones  precursoras y el cáncer cérvico  uterino” | Uniparts - Biología Molecular | Scoop.it

SOCIEDAD MEXICANA DEL VIRUS DEL PAPILOMA A.C.

2a. Reunión Ordinaria

 

Fecha: 14 de Diciembre del 2012

Sede: Auditorio-3 del Centro Medico Nacional IMSS

(Para personal médico y paramédico)

Cuota de Inscripción $ 400.00

 

"Preguntas más frecuentes sobre la Infección por el Virus de Papiloma Humano"

Fecha: 13 de Diciembre del 2012

(Invitación abierta y gratuita a la comunidad)

Informes: 55646412 www.smvp.infored.mx

ojiverde_358@hotmail.com

 

Programa:

http://www.smvp.infored.mx/imagesnew2/0/0/0/1/1/1/1/3/0/9/REUNION_ORDINARIA_FIN.pdf

 

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Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses

Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses | Uniparts - Biología Molecular | Scoop.it

Abstract

Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were antisense to protein-coding mRNAs, suggesting cis-regulatory roles. Numerous 24-nt siRNA matched to five different npcRNAs, suggesting that these npcRNAs are precursors of this type of siRNA. Expression analyses of the 76 npcRNAs identified a novel npcRNA that accumulates in a dcl1 mutant but does not appear to produce trans-acting siRNA or miRNA. Additionally, another npcRNA was the precursor of miR869 and shown to be up-regulated in dcl4 but not in dcl1 mutants, indicative of a young miRNA gene. Abiotic stress altered the accumulation of 22 npcRNAs among the 76, a fraction significantly higher than that observed for the RNA binding protein-coding fraction of the transcriptome. Overexpression analyses in Arabidopsis identified two npcRNAs as regulators of root growth during salt stress and leaf morphology, respectively. Hence, together with small RNAs, long npcRNAs encompass a sensitive component of the transcriptome that have diverse roles during growth and differentiation.


Via Biswapriya Biswavas Misra, Andres Zurita
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The implications of ENCODE for diagnostics

The implications of ENCODE for diagnostics | Uniparts - Biología Molecular | Scoop.it
Large-scale functional genomics data coupled with whole-genome sequencing will lead to improved diagnostic assays.

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