Biosynthesis, Natural Products and Synthetic Biology
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Rescooped by Fred Dudbridge from Natural Products Chemistry Breaking News
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Structure of a modular polyketide synthase

Structure of a modular polyketide synthase | Biosynthesis, Natural Products and Synthetic Biology | Scoop.it

Polyketide natural products constitute a broad class of compounds with diverse structural features and biological activities. Their biosynthetic machinery, represented by type I polyketide synthases (PKSs), has an architecture in which successive modules catalyse two-carbon linear extensions and keto-group processing reactions on intermediates covalently tethered to carrier domains. Here we used electron cryo-microscopy to determine sub-nanometre-resolution three-dimensional reconstructions of a full-length PKS module from the bacterium Streptomyces venezuelae that revealed an unexpectedly different architecture compared to the homologous dimeric mammalian fatty acid synthase. A single reaction chamber provides access to all catalytic sites for the intramodule carrier domain. In contrast, the carrier from the preceding module uses a separate entrance outside the reaction chamber to deliver the upstream polyketide intermediate for subsequent extension and modification. This study reveals for the first time, to our knowledge, the structural basis for both intramodule and intermodule substrate transfer in polyketide synthases, and establishes a new model for molecular dissection of these multifunctional enzyme systems.

 

Somnath Dutta,Jonathan R. Whicher,Douglas A. Hansen,Wendi A. Hale,Joseph A. Chemler,Grady R. Congdon,Alison R. H. Narayan,Kristina Håkansson,David H. Sherman,Janet L. Smith& Georgios SkiniotisAffiliationsContributionsCorresponding authorNature(2014)doi:10.1038/nature13423Received07 May 2013Accepted01 May 2014
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Rescooped by Fred Dudbridge from Synthetic Biology
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Tiny Bacteria Provide Genetics to Save Food

Tiny Bacteria Provide Genetics to Save Food | Biosynthesis, Natural Products and Synthetic Biology | Scoop.it

Plants are often thought of as the masters of photosynthesis, the process by which sunlight, carbon dioxide and water are converted into usable energy, but when it comes to efficiency, they are beaten out by a rather surprising rival: bacteria.


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Rakesh Yashroy's curator insight, June 21, 2014 6:12 AM

Awsome twosome - sharing genes between plants and bacterial for higher crop productivity.

Rescooped by Fred Dudbridge from Synthetic Biology
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Promising solution to plastic pollution

Promising solution to plastic pollution | Biosynthesis, Natural Products and Synthetic Biology | Scoop.it

For many people, “plastic” is a one-word analog for environmental disaster. It is made from precious petroleum, after all, and once discarded in landfills and oceans, it takes centuries to degrade.


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Rescooped by Fred Dudbridge from Synthetic Biology
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From foundry to factory: building synthetic plants

From foundry to factory: building synthetic plants | Biosynthesis, Natural Products and Synthetic Biology | Scoop.it

A movement is under way that will fast-forward the design of new plant traits. It takes inspiration from engineering and the software industry, and is being underpinned in Cambridge and Norwich by an initiative called OpenPlant. 


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