SynBioFromLeukipp...
Follow
Find
86.4K views | +22 today
 
Scooped by Socrates Logos
onto SynBioFromLeukipposInstitute
Scoop.it!

Reprogramming in vivo produces teratomas and iPS cells with totipotency features

Reprogramming in vivo produces teratomas and iPS cells with totipotency features | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

by
María Abad,Lluc Mosteiro,Cristina Pantoja,Marta Cañamero,Teresa Rayon,Inmaculada Ors,Osvaldo Graña,Diego Megías,Orlando Domínguez,Dolores Martínez,Miguel Manzanares,Sagrario Ortega& Manuel Serrano

"Reprogramming of adult cells to generate induced pluripotent stem cells (iPS cells) has opened new therapeutic opportunities; however, little is known about the possibility of in vivo reprogramming within tissues. Here we show that transitory induction of the four factors Oct4, Sox2, Klf4 and c-Myc in mice results in teratomas emerging from multiple organs, implying that full reprogramming can occur in vivo. Analyses of the stomach, intestine, pancreas and kidney reveal groups of dedifferentiated cells that express the pluripotency marker NANOG, indicative of in situ reprogramming. By bone marrow transplantation, we demonstrate that haematopoietic cells can also be reprogrammed in vivo. Notably, reprogrammable mice present circulating iPS cells in the blood and, at the transcriptome level, these in vivo generated iPS cells are closer to embryonic stem cells (ES cells) than standard in vitro generated iPS cells. Moreover, in vivo iPS cells efficiently contribute to the trophectoderm lineage, suggesting that they achieve a more plastic or primitive state than ES cells. Finally, intraperitoneal injection of in vivo iPS cells generates embryo-like structures that express embryonic and extraembryonic markers. We conclude that reprogramming in vivo is feasible and confers totipotency features absent in standard iPS or ES cells. These discoveries could be relevant for future applications of reprogramming in regenerative medicine."


http://bit.ly/1gqWvgQ

more...
Adrian Vallejo Blanco's curator insight, November 3, 2013 5:12 PM

Se consigue por primera vez obtener células totipotenciales (iPS) en modelos in vivo. Ya no es novedad haber conseguido células iPS en experimentos in vitro pero en modelos animales in vivo aún era una quimera. Induciendo ciertos factores se ha conseguido identificar en el estómago células que expresan marcadores pluripotenciales como NANOG. En trasplantes de médula ósea se sabe que las células hematopoyéticas pueden reprogramarse in vivo.

 

Los ratones reprogramables presentan circulación de células iPS en la sangre, células generadas in vivo que a nivel del transcriptoma, se acercan más a las células madre embrionarias (células ES) que las que han sido reprogramadas in vitro.

 

Esta mejor capacidad de las reprogramadas in vivo de alcanzar las características de las células madre embrionarias que las reprogramadas in vitro confieren un futuro muy prometedor en lo que se refiere a medicina regenerativa.

SynBioFromLeukipposInstitute
Your new post is loading...
Your new post is loading...
Scooped by Socrates Logos
Scoop.it!

James P. Collins - One Biology, One Science: A Vision for the 21st Century - YouTube

Space exploration is limited by the cost of launching materials off earth. Biology could solve this problem making possible the dream of producing bespoke to...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Abeona Therapeutics using CRISPR to treat Fanconi anemia, raises $8.5M

Abeona Therapeutics using CRISPR to treat Fanconi anemia, raises $8.5M | SynBioFromLeukipposInstitute | Scoop.it
Abeona Therapeutics using CRISPR to treat Fanconi anemia, raises $8.5M
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

New Chlamydia Vaccine Uses Synthetic Biology

New Chlamydia Vaccine Uses Synthetic Biology | SynBioFromLeukipposInstitute | Scoop.it
Chlamydia is one of the most common sexually transmitted infections in the world.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic Biology for Space Program - Christopher Carr - YouTube

Source - http://serious-science.org/videos/1080 MIT Research Scientist Christopher Carr on challenges of distant journeys, components of cosmic radiation, an...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic biology: Ribosomal ties that bind

The ribosome is the cellular complex of proteins and RNA molecules that synthesizes proteins. An artificial ribosome in which the two main subunits are tethered together creates opportunities for engineering this process.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

UC Irvine to exhibit artworks created via biological engineering

UC Irvine to exhibit artworks created via biological engineering | SynBioFromLeukipposInstitute | Scoop.it
UC Irvine has put out a call for artists who want to manipulate the building blocks of life as we know it to create art as we’ve never known it – works made of living organisms that owe their existence to biological engineering rather...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Continuous microfluidic fabrication of synthetic asymmetric vesicles.

Continuous microfluidic fabrication of synthetic asymmetric vesicles. | SynBioFromLeukipposInstitute | Scoop.it
Lab Chip. 2015 Jul 29. [Epub ahead of print]
Socrates Logos's insight:

"We report on a novel microfluidic strategy for the continuous fabrication of monodisperse asymmetric vesicles with customized membrane composition, size, and luminal content. The microfluidic device encompasses a triangular post region and two flow-focusing regions. The major steps involved in the vesicle fabrication process include: (1) forming highly uniform water emulsions in an oil/inner-leaflet-lipid solution, (2) replacing the inner-leaflet-lipid solution with an outer-leaflet-lipid solution inside the microchannel network, (3) forming water-in-oil-in-water double emulsions, and (4) extracting excess oil/outer-leaflet-lipid solution from the double emulsions. Bilayer membrane asymmetry and unilamellarity are evaluated using a fluorescence quenching assay and a transmembrane protein insertion assay, respectively. Our approach addresses many of the deficiencies found in existing technologies for building vesicles, and yields strong membrane asymmetry. The ability to create and sustain membrane asymmetry is an important feature, as it is a characteristic of nearly all natural membranes. Over 80% of the vesicles remain stable for at least 6 weeks and the membrane asymmetry is maintained for over 30 hours. The asymmetric vesicles built using this strategy are collected off-chip and hold the potential to be used as model systems in membrane biology or as vehicles for drug delivery.'

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Is synthetic biology mechanical biology? - PubMed - NCBI

Is synthetic biology mechanical biology? - PubMed - NCBI | SynBioFromLeukipposInstitute | Scoop.it
Hist Philos Life Sci. 2015 Jul 24. [Epub ahead of print]
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic biology, future trends to look out for. Cathal Garvey, Indie Bio Sci-director

Synthetic biology, future trends to look out for. Cathal Garvey, Indie Bio Sci-director | SynBioFromLeukipposInstitute | Scoop.it
By @SimonCockingReally pleased to bring you the words and insights of Cathal Garvey @onetruecathal, Sci-Director of Indie Bio in Cork, EU Synbio accelerator. As well as DIYbio enabler, Pythonista
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Optical Control of CRISPR/Cas9 Gene Editing

Optical Control of CRISPR/Cas9 Gene Editing | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

by
James Hemphill, Erin K. Borchardt, Kalyn Brown, Aravind Asokan, and Alexander Deiters 

"The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gene therapy. In order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activated Cas9 was engineered through the site-specific installation of a caged lysine amino acid. Several potential lysine residues were identified as viable caging sites that can be modified to optically control Cas9 function, as demonstrated through optical activation and deactivation of both exogenous and endogenous gene function."
 http://bit.ly/1Mn2lOh

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Towards synthetic biological approaches to resource utilization on space missions

Towards synthetic biological approaches to resource utilization on space missions | SynBioFromLeukipposInstitute | Scoop.it
RT @RSocPublishing: Can synthetic biology help future space exploration? http://t.co/eGJDgMhCU3 #ukspace2015 http://t.co/amw2DO8slU
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

How are nature and computers related? - YouTube

Socrates Logos's insight:

A platonic idea is an archetype, a blueprint, the essence of various phenomena of the same thing. 

Systemics and systems biology are such ideas, describing data processing systems in nature in terms of mathematics and formal logic. Systemic ideas have been used as a blueprint for silicon computing. Ideas derived from the observation of nature have also inspired computer models of nature. 

Engineering ideas behind silicon computer (such as standardized parts, switches, logic gates, input /output device, arithmetic logic unit, control unit, memory, and busses) have been used by synthetic biologists to build computers with biological parts, with the ultimate goal to control data processing in nature.


BTW

Our Leukippos community needs your financial support. We are all working for free and pay everything out of our own pockets, such as server costs and page registrations. If you like our work and wish that we produce more quality content, such as this video, you can donate some bitcoins to this address: 


1KnikzSG7fnRfG76DxjLZyrbvw8fS9nisw


Further info:

Review - THE BIOLOGICAL MICROPROCESSOR, OR HOW TO BUILD A COMPUTER WITH BIOLOGICAL PARTS http://bit.ly/YI13bF

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Makers Beyond Earth: Synthetic Biology and Space Exploration - YouTube

Space exploration is limited by the cost of launching materials off earth. Biology could solve this problem making possible the dream of producing bespoke to...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic ribosome can keep bacteria alive « AlwaysResearching.com

Synthetic ribosome can keep bacteria alive « AlwaysResearching.com | SynBioFromLeukipposInstitute | Scoop.it
View the article's original source Author: Megan Fellman-Northwestern Scientists have engineered a tethered ribosome that works nearly as well as the real thing—an organelle that produces all the proteins and enzymes within the cell.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

€41M for a Rising Star of Synthetic Biology: Ginkgo Bioworks - Labiotech.eu

€41M for a Rising Star of Synthetic Biology: Ginkgo Bioworks - Labiotech.eu | SynBioFromLeukipposInstitute | Scoop.it
Once will not hurt, we’ll speak a bit about an inspiring US-based company. A synthetic biology start-up from the worlds biggest biocluster is on its road to success.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

The Genesis Engine

Editing DNA is now cut-and-paste. We could eliminate disease, cure hunger—or break the world.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

The exciting future of synthetic biology, Ryan Bethencourt Indie Bio innovator

The exciting future of synthetic biology, Ryan Bethencourt Indie Bio innovator | SynBioFromLeukipposInstitute | Scoop.it
By @SimonCockingReally interesting interview with Indie Bio innovator Ryan Bethencourt @RyanBethencourt  Program Director & VC Partner at IndieBio San Francisco @indbio. Also former Head o
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic biology: Ribosomal ties that bind - Nature.com

Nature.com
Synthetic biology: Ribosomal ties that bind
Nature.com
Biochemists have been engineering biomolecules for decades — proteins with new folds and functions, and nucleic acids that control gene expression or form complex architectures.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Bacteria Programmed to Develop Basic Computing Elements Like Sensors, Memory and Circuits

Bacteria Programmed to Develop Basic Computing Elements Like Sensors, Memory and Circuits | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

"The researchers at the Massachusetts Institute of Technology have successfully programmed the friendly bacteria inside our body to detect diseases like colon cancer and immune disorder- and treat them. They have unveiled sensors, circuits, and memory switches to be encoded in bacterium Bacteroides thetaiotaomicron, found in human gut system."

http://bit.ly/1LLTpl4

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Modulating protein activity using tethered ligands with mutually exclusive binding sites

Modulating protein activity using tethered ligands with mutually exclusive binding sites | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

by
Alberto Schena, Rudolf Griss & Kai Johnsson

"The possibility to design proteins whose activities can be switched on and off by unrelated effector molecules would enable applications in various research areas, ranging from biosensing to synthetic biology. We describe here a general method to modulate the activity of a protein in response to the concentration of a specific effector. The approach is based on synthetic ligands that possess two mutually exclusive binding sites, one for the protein of interest and one for the effector. Tethering such a ligand to the protein of interest results in an intramolecular ligand–protein interaction that can be disrupted through the presence of the effector. Specifically, we introduce a luciferase controlled by another protein, a human carbonic anhydrase whose activity can be controlled by proteins or small molecules in vitro and on living cells, and novel fluorescent and bioluminescent biosensors."

 http://bit.ly/1RU8shG

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Jim Collins '87 gives lecture on synthetic biology

Holy Cross alumnus James J. Collins '87 is a pioneer in the emerging field of synthetic biology, which combines biology and engineering to create circuits that ...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

A Guide for Communicating Synthetic Biology - Synthetic Biology Project

A Guide for Communicating Synthetic Biology - Synthetic Biology Project | SynBioFromLeukipposInstitute | Scoop.it
Ensuring benefits of synthetic biology are realized through responsible development. Synthetic biology specific news, events, publications and more.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

CytoComp - YouTube

CytoComp - YouTube | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

I made 16 brief videos explaining a biological microprocessor 

more...
No comment yet.