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In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation | SynBioFromLeukipposInstitute | Scoop.it
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Michael C Jewett, Brian R Fritz, Laura E Timmerman & George M Church

"This report describes an integrated method for in vitro construction of Escherichia coli ribosomes under near-physiological conditions. This method enables coupling of ribosome synthesis and assembly in a single, integrated system.

An integrated synthesis, assembly, and translation technology (termed iSAT) was developed to construct ribosomes in vitro.iSAT mimics co-transcription of rRNA and ribosome assembly as it occurs in vivo.iSAT makes possible the in vitro construction of modified ribosomes.iSAT is expected to aid studies of ribosome assembly and open new avenues for making ribosomes with altered capabilities.Top of pageAbstractPurely in vitro ribosome synthesis could provide a critical step towards unraveling the systems biology of ribosome biogenesis, constructing minimal cells from defined components, and engineering ribosomes with new functions. Here, as an initial step towards this goal, we report a method for constructing Escherichia coli ribosomes in crude S150 E. coli extracts. While conventional methods for E. coli ribosome reconstitution are non-physiological, our approach attempts to mimic chemical conditions in the cytoplasm, thus permitting several biological processes to occur simultaneously. Specifically, our integrated synthesis, assembly, and translation (iSAT) technology enables one-step co-activation of rRNA transcription, assembly of transcribed rRNA with native ribosomal proteins into functional ribosomes, and synthesis of active protein by these ribosomes in the same compartment. We show that iSAT makes possible the in vitro construction of modified ribosomes by introducing a 23S rRNA mutation that mediates resistance against clindamycin. We anticipate that iSAT will aid studies of ribosome assembly and open new avenues for making ribosomes with altered properties."


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Convergence of regenerative medicine and synthetic biology to develop standardized and validated models of human diseases with clinical relevance

Convergence of regenerative medicine and synthetic biology to develop standardized and validated models of human diseases with clinical relevance | SynBioFromLeukipposInstitute | Scoop.it
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Why Bio is the New Digital - Joi Ito keynote - YouTube

From the 2015 Solid Conference. About Joichi Ito (MIT Media Lab): Media Lab Director Joi Ito is a leading thinker and writer on innovation, global technology...
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Dr. Alicia Jackson: Programming the Living World, DARPA BiT

Dr. Alicia Jackson, Deputy Director of DARPA's Biological Technologies Office (BTO), discusses the potential of synthetic biology and describes new tools that ...
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Building a Fab for Synthetic Biology - Joe Jacobson keynote

From Solid Conference 2015: Similar to the way in which we use fabs to build microprocessors and software to program them, the field of synthetic biology offers ...
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Synthetic biology used to engineer new route to biochemicals

Synthetic biology used to engineer new route to biochemicals | SynBioFromLeukipposInstitute | Scoop.it
Living cells can make a vast range of products for us, but they don't always do it in the most straightforward or efficient way.
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What are the building blocks of a biological microprocessor? - YouTube

What are the building blocks of a biological microprocessor? A biological microprocessor consist of four units: the input and output device (I/O), the arithm...
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Synthetic Biology and Microbiome Engineering approaches for Trimethylaminuria

Jake Wintermute, Ph.D. Postdoctoral Researcher, Paris Descartes University For more information, join the international TMAU community on RareConnect: ...
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What are biological microprocessor applications? Part 1 - YouTube

Monitoring of biological systems by a biological microprocessor

Biological systems need to be monitored in respect to disease diagnostic, to drug screening, to understand experimental systems, and to observe the environment.

In line with this, a biocomputer has been utilized to detect multiple disease indicators, such as mRNA of disease-related genes associated with small-cell lung cancer and prostate cancer . Moreover, they can be used in experimental models, such as conditional transgenes or inducible expression systems. Environmental monitoring is another interesting application. A cell based biosensor using logic gates has been used to detect arsenic, mercury and copper ion levels.

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Synthetic Biology: DNA origami

RT @ICO2S: #ICOSseminar "Synthetic Biology: DNA origami" by Chien Yi Chang (2015-06-23) http://t.co/mDrkVQMuCN
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Steve Evans

Steve Evans speaks on the promises and challenges facing Engineering Biology (synthetic biology) in the U.S..
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A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future

A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future | SynBioFromLeukipposInstitute | Scoop.it
A renaissance in #RNA synthetic biology: new mechanisms, applications and tools for the future: James… http://t.co/BnND0ELmcZ #COin
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High-Throughput Synthetic Biology: Synthesizing Designer Yeast through Automation and Acoustic Liquid Handling | Webinars | GEN

High-Throughput Synthetic Biology: Synthesizing Designer Yeast through Automation and Acoustic Liquid Handling | Webinars | GEN | SynBioFromLeukipposInstitute | Scoop.it
Get the latest in biotechnology through daily news coverage as well as analysis, features, tutorials, webinars, podcasts, and blogs. Learn about the entire bioproduct life cycle from early-stage R&D, to applied research including omics, biomarkers, as well as diagnostics, to bioprocessing and commercialization.
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Systems Biology

Systems Biology Adam Arkin, University of California, Berkeley and E.O. Lawrence Berkeley National Laboratory Over eons, evolution acts on organisms to ...
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Download PDF Microbial Synthetic Biology Volume 40

1.Browse And Download This Book now. 2.If you can't download change your ip adress. 3."Click here to download PDF Ebook now at http://bit.ly/1Kgs4Xk.
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How does a biological Input:/Output device work? - YouTube

Input/Output (I/O) device: In a “digital” biological I/O device input molecules induce due to a set of non-steady state chemical reactions (engineered cohere...
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Jay Keasling - 18th Heinz Awards - Technology, the Economy and Employment

Jay Keasling, our 18th Technology, the Economy and Employment recipient, talks about the power of synthetic biology, and how his work on the new science of ...
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T Cell Immunotherapy: From Synthetic Biology to Clinical Practice - Springer

T Cell Immunotherapy: From Synthetic Biology to Clinical Practice - Springer | SynBioFromLeukipposInstitute | Scoop.it
T Cell Immunotherapy: From Synthetic Biology to Clinical Practice - Springer http://t.co/oGF6fKBYvW
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What are biological microprocessor applications? Part 2 - YouTube

What are biological microprocessor applications? Part 2
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Membranes grow like living cells

Membranes grow like living cells | SynBioFromLeukipposInstitute | Scoop.it
UC San Diego researchers have replicated a key feature of life in synthetic membranes.
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Howard Salis - Engineering Biology

Howard Salis speaks on the promises and challenges facing Engineering Biology (synthetic biology) in the U.S..
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Synthetic Biology Gets Inspiration from the Sea | Synthetic Biology Community

Synthetic Biology Gets Inspiration from the Sea | Synthetic Biology Community | SynBioFromLeukipposInstitute | Scoop.it
by Olga Radchuck and Markus Schmidt (Biofaction, Austria) A solution for the problem of antibiotic resistance, threatening public health, may come from an
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