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University of Washington #SynBio web portal http://bit.ly/YWPHc8
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"Custom-defined oligonucleotide collections have a broad range of applications in fields of synthetic biology, targeted sequencing, and cytogenetics. Also, they are used to encode information for technologies like RNA interference, protein engineering and DNA-encoded libraries. High-throughput parallel DNA synthesis technologies developed for the manufacture of DNA microarrays can produce libraries of large numbers of different oligonucleotides, but in very limited amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with a biotinylated strand captured on streptavidin coated magnetic beads results in little or no non-biotinylated ssDNA. The second method wherein the phosphorylated strand of PCR amplified libraries is nucleolyticaly hydrolyzed is recommended when small amounts of libraries are needed. The third method combining in vitro transcription of PCR amplified libraries to reverse transcription of the RNA product into single-stranded cDNA is our recommended method to produce large amounts of oligonucleotide libraries. Finally, we propose a method to remove any primer binding sequences introduced during library amplification."http://bit.ly/1m8jlvN
Biomedical engineer James Collins of Boston and Harvard universities and the Howard Hughes Medical Institute, will give a BSA Distinguished Lecture, titled "Life Redesigned: The Emergence of Synthetic Biology," at the U.S. Department of Energy's Brookhaven National Laboratory on Wednesday, April 30.
byEduardo Antonio Della Pia, Randi Westh Hansen, Manuela Zoonens, Karen L. Martinez"Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches."http://bit.ly/1h0r7QB
Five things you should know about this growing segment that aims to modify life itself.
Question to all Entrepreneurs: http://bit.ly/1gWLBtp
byArthur Prindle, Jangir Selimkhanov, Howard Li, Ivan Razinkov, Lev S. Tsimring & Jeff Hasty"One promise of synthetic biology is the creation of genetic circuitry that enables the execution of logical programming in living cells. Such ‘wet programming’ is positioned to transform a wide and diverse swathe of biotechnology ranging from therapeutics and diagnostics to water treatment strategies. Although progress in the development of a library of genetic modules continues apace1, 2, 3, 4, a major challenge for their integration into larger circuits is the generation of sufficiently fast and precise communication between modules5, 6. An attractive approach is to integrate engineered circuits with host processes that facilitate robust cellular signalling7. In this context, recent studies have demonstrated that bacterial protein degradation can trigger a precise response to stress by overloading a limited supply of intracellular proteases8, 9, 10. Here we use protease competition to engineer rapid and tunable coupling of genetic circuits across multiple spatial and temporal scales. We characterize coupling delay times that are more than an order of magnitude faster than standard transcription-factor-based coupling methods (less than 1 min compared with ~20–40 min) and demonstrate tunability through manipulation of the linker between the protein and its degradation tag. We use this mechanism as a platform to couple genetic clocks at the intracellular and colony level, then synchronize the multi-colony dynamics to reduce variability in both clocks. We show how the coupled clock network can be used to encode independent environmental inputs into a single time series output, thus enabling frequency multiplexing (information transmitted on a common channel by distinct frequencies) in a genetic circuit context. Our results establish a general framework for the rapid and tunable coupling of genetic circuits through the use of native ‘queueing’ processes such as competitive protein degradation." http://bit.ly/1hGY7NV
"First International Workshop on Plant Synthetic Biology" #synbio http://t.co/BAbKfZ5efb
byYue Zhang"Many pioneering works have inspired researchers to stay up-todate on synthetic and system biology. Several cases that were originally thought to be exceptionally difficult, if not impossible, have been carried out successfully, such as Craig Venter’s creation of the world’s first synthetic life form. At a system level, nucleic reprogramming succeeded in frog half a century ago (reviewed in ); but doubts about whether or not this was impossible lingered until 40 years later, when a cocktail of four transcriptional factors systematically reprogrammed the somatic cells to stem cells [1-3]. Other cases include that telomerase reactivation may lead to the reversal of tissue degeneration in aged telomerase-deficient mice  and muscle-derived stem/progenitor cell dysfunction acts as a healthspan and lifespan limiting factor for murine progeria reversal .
(Phys.org) —Synthetic genetic circuitry created by researchers at Rice University is helping them see, for the first time, how to regulate cell mechanisms that degrade the misfolded proteins implicated in Parkinson's, Huntington's and other diseases.
Read about how Mathematicians and Biochemists Synthesize a Temperature-Invariant Biological Genetic Clock at UH and Rice
Nanowerk Berkeley Lab proposal for an open biofoundry passes crucial first test Nanowerk This biomanufacturing center would aim to meet the biomanufacturing challenges through three main interrelated components: a one-of-a-kind open collaboration...
Forget sustainability -- 13 of the world's leading tire manufacturers are eyeing Amyris' synthetic biology platform for a completely different reason. - Maxx Chatsko - Energy
Nonprofit Organization for LOW COST, OPEN SOURCE, 3D PRINTABLE Biomedical Technologies
LOUISVILLE, Ky. (AP) -- It may sound far-fetched, but scientists are attempting to build a human heart with a 3-D printer.Ultimately, the goal is to create a new heart for a patient with their own cells that could be transplanted. It is an ambitious project to first, make a heart and then get it to work in a patient, and it could be years — perhaps decades — before a 3-D printed heart would ever be put in a person.The technology, though, is not all that futuristic: Researchers have already used 3-D printers to make splints, valves and even a human ear.
byRicard Solé & Javier Macía"Cellular biocircuit design has taken a major step forward. The circuit reuses the cell's own protein-degradation system to synchronize the expression of two synthetic modules throughout an entire bacterial population."http://bit.ly/1hqOKqu
Scientists grew reproductive organs and nasal cartilage in labs, and later successfully implanted them in patients, according to two studies released Thursday.
Programming living cells offers the prospect of harnessing sophisticated biological machinery for transformative applications in energy, agriculture, water remediation and medicine. Inspired by engineering, researchers in the emerging field of synthetic biology have designed a tool box of small genetic ...
Gives a whole new meaning to the term "computer bug."
There are very few, if any discoveries each year in academia that come about without building on the concepts and ideas that have been previously publ...
*Academica is broken*by
MarkHahnel"There are very few, if any discoveries each year in academia that come about without building on the concepts and ideas that have been previously published in academic journals. This is the natural progression of research. However, this is often limited to building on top of conclusions or ideas, as opposed to conducting the actual research itself. Current dissemination of research is largely based on making .pdf-based summaries of key findings available, while the actual research outputs and raw data behind the graphs are largely unavailable. This isn’t due to a lack of demand to by researchers to get credit for all of their hard work, it’s because the publication and subsequent reward structure in academia does not support this.
CTV NewsHow these London scientists make body parts in a labThe Times HeraldHow these London scientists make body parts in a lab.