SynBioFromLeukipp...
Follow
Find
87.4K views | +57 today
 
Scooped by Socrates Logos
onto SynBioFromLeukipposInstitute
Scoop.it!

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

RNA-guided editing of bacterial genomes using CRISPR-Cas systems | SynBioFromLeukipposInstitute | Scoop.it
A CRISPR-Cas system is harnessed to introduce template-driven mutations in S. pneumoniae and E. coli at high efficiency without requiring selectable markers.
Socrates Logos's insight:

Congratulation +David Bikard 

*RNA-guided editing of bacterial genomes using CRISPR-Cas systems*

by
Wenyan Jiang,David Bikard,David Cox,Feng Zhang& Luciano A Marraffini

"Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the targeted genomic site to kill unmutated cells and circumvents the need for selectable markers or counter-selection systems. We reprogram dual-RNA:Cas9 specificity by changing the sequence of short CRISPR RNA (crRNA) to make single- and multinucleotide changes carried on editing templates. Simultaneous use of two crRNAs enables multiplex mutagenesis. In S. pneumoniae, nearly 100% of cells that were recovered using our approach contained the desired mutation, and in E. coli, 65% that were recovered contained the mutation, when the approach was used in combination with recombineering. We exhaustively analyze dual-RNA:Cas9 target requirements to define the range of targetable sequences and show strategies for editing sites that do not meet these requirements, suggesting the versatility of this technique for bacterial genome engineering."

http://bit.ly/11dWGDK

see also http://bit.ly/VvkdOY

more...
No comment yet.
SynBioFromLeukipposInstitute
Your new post is loading...
Your new post is loading...
Scooped by Socrates Logos
Scoop.it!

Programmable genetic circuits for pathway engineering

Programmable genetic circuits for pathway engineering | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

by

Allison Hoynes-O’Connor, Tae Seok Moon 

"Highlights

•Genetic tools from synthetic biology hold potential for pathway engineering.
•With metabolic and environmental sensors, cells can respond to their conditions.
•Genetic circuits connect cellular conditions with the appropriate response.
•Multi-gene targeting allows simultaneous, orthogonal regulation of multiple genes.
•Integration of these three components will lead to advances in pathway engineering."
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Engineering Synthetic cis-Regulatory Elements for Simultaneous Recognition of Three Transcriptional Factors

Engineering Synthetic cis-Regulatory Elements for Simultaneous Recognition of Three Transcriptional Factors | SynBioFromLeukipposInstitute | Scoop.it
Gerardo Ruiz Amores, María-Eugenia Guazzaroni and Rafael Silva-Rocha
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Emergent genetic oscillations in a synthetic microbial consortium | CodonOps

Emergent genetic oscillations in a synthetic microbial consortium | CodonOps | SynBioFromLeukipposInstitute | Scoop.it
Emergent genetic oscillations in a synthetic microbial consortium
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

KAIST's mathematician reveals the mechanism for sustaining biological rhythms

KAIST's mathematician reveals the mechanism for sustaining biological rhythms | SynBioFromLeukipposInstitute | Scoop.it
the mechanism for sustaining biological rhythms
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Tissue Engineering, Synthetic Biology & Bioprinting

Tissue Engineering, Synthetic Biology & Bioprinting | SynBioFromLeukipposInstitute | Scoop.it
Tissue Engineering Synthetic Biology Biofabrication Bioprinting 3D-Printing
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Researchers use DNA 'nanoclews' to shuttle CRISPR-Cas9 gene-editing tool into cells

Researchers use DNA 'nanoclews' to shuttle CRISPR-Cas9 gene-editing tool into cells | SynBioFromLeukipposInstitute | Scoop.it
Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have for the first time created and used a nanoscale vehicle made of DNA to deliver a CRISPR-Cas9 gene-editing tool into cells in both cell culture and an animal model.
The CRISPR-Cas system, which is found in bacteria and archaea, protects bacteria from invaders such as viruses. It does this by creating small strands of RNA called CRISPR RNAs, which match DNA sequences specific to a given invader. When those CRISPR RNAs find a match, they unleash Cas9 proteins that cut the DNA. In recent years, the CRISPR-Cas system has garnered a great deal of attention in the research community for its potential use as a gene editing tool - with the CRISPR RNA identifying the targeted portion of the relevant DNA, and the Cas protein cleaving it.
But for Cas9 to do its work, it must first find its way into the cell. This work focused on demonstrating the potential of a new vehicle for directly introducing the CRISPR-Cas9 complex - the entire gene-editing tool - into a cell.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Scientists modify E. coli to cooperate, control protein expression

Scientists modify E. coli to cooperate, control protein expression | SynBioFromLeukipposInstitute | Scoop.it
Rice University scientists have made a living circuit from multiple types of bacteria that prompts the bacteria to cooperate to change protein expression.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Minority rules: Scientists discover tipping point for the spread of ideas

Minority rules: Scientists discover tipping point for the spread of ideas | SynBioFromLeukipposInstitute | Scoop.it
Minority rules: Scientists discover tipping point for the spread of ideas
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Harnessing Synthetic Biology to Combat Bacterial Pathogens - DVIDS (press release)

Harnessing Synthetic Biology to Combat Bacterial Pathogens - DVIDS (press release) | SynBioFromLeukipposInstitute | Scoop.it
Developed in the 1940s, antibiotics are powerful medicines used in the treatment and prevention of bacterial infection to save lives.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Accurate DNA Assembly and Genome Engineering with
Optimized Uracil Excision Cloning | CodonOps

Accurate DNA Assembly and Genome Engineering with<br/>Optimized Uracil Excision Cloning | CodonOps | SynBioFromLeukipposInstitute | Scoop.it
Ana Mafalda Cavaleiro, Se Hyeuk Kim, Susanna Seppälä, Morten T. Nielsen and Morten H. H. Nørholm
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Biohackers gear up for genome editing

Biohackers gear up for genome editing | SynBioFromLeukipposInstitute | Scoop.it
Amateurs are ready and able to try the CRISPR technique for rewriting genes.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic Biology Could Let Us Recycle Human Waste For Space Travel - The Escapist

The Escapist
Synthetic Biology Could Let Us Recycle Human Waste For Space Travel
The Escapist
One day, astronauts might recycle urine and carbon dioxide into highly necessary food and medicines for space missions.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Emergent genetic oscillations in a synthetic microbial consortium.

A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types-an "activator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types.
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

DNA-guided 3-D printing of human tissue is unveiled

DNA-guided 3-D printing of human tissue is unveiled | SynBioFromLeukipposInstitute | Scoop.it
DNA-guided 3-D printing of human tissue is unveiled
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

The Evolution of Genome Engineering - YouTube

Genome editing tools combine molecular biology and engineering principles, enabling researchers to study how the genome influences phenotype. This video illu...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Synthetic biology needs a grand design vision - New Scientist

Synthetic biology needs a grand design vision - New Scientist | SynBioFromLeukipposInstitute | Scoop.it
We can design a better future using biology, but we must consider the big questions first, says Alexandra Daisy Ginsberg
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Caution urged over editing DNA in wildlife (intentionally or not)

Caution urged over editing DNA in wildlife (intentionally or not) | SynBioFromLeukipposInstitute | Scoop.it
Caution urged over editing DNA in wildlife (intentionally or not)
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Emergent genetic oscillations in a synthetic microbial consortium

Emergent genetic oscillations in a synthetic microbial consortium | SynBioFromLeukipposInstitute | Scoop.it
Socrates Logos's insight:

by
Ye Chen, Jae Kyoung Kim, Andrew J. Hirning, Krešimir Josić, Matthew R. Bennett

"A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types—an “activator” strain and a “repressor” strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types."

http://bit.ly/1KpKUfy

Image
http://phys.org/news/2015-08-scientists-coli-cooperate-protein.html

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Searching big data faster

Searching big data faster | SynBioFromLeukipposInstitute | Scoop.it
Searching big data faster
Socrates Logos's insight:

"Theoretical analysis could expand applications of accelerated searching in biology, other fields."

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

So You Think You Can Synthesise - Series Finale

The Macquarie_Australia iGEM Team uses the popular reality format to inform and engage the public with our project The Solar Synthesisers. Our Project aims ...
more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Researchers Want to Inject 3D Printed Microfish Into Your System

Researchers have developed a 3D printing technology that can produce microfish (fish-shaped microrobots) to be used for medical purposes.
Socrates Logos's insight:

Is this the future of medicine?

more...
No comment yet.
Scooped by Socrates Logos
Scoop.it!

Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation

Nature Communications | doi:10.1038/ncomms8978
more...
No comment yet.