Synbio
19 views | +0 today
Follow
Your new post is loading...
Your new post is loading...
Rescooped by Kartik Soni from Synthetic Biology
Scoop.it!

Scientists Create First Living Organism With 'Artificial' DNA - Huffington Post

Scientists Create First Living Organism With 'Artificial' DNA - Huffington Post | Synbio | Scoop.it

 

Scientists first questioned whether life could store information using other chemical groups than A, C, G and T in the 1960s. But it wasn’t until 1989 that Steven Benner, coaxed modified forms of cytosine (C) and guanine (G) into DNA molecules. In test-tube reactions, strands made of these “funny letters”, as Benner calls them, copied themselves and encoded RNA and proteins.

 

The bases engineered by Romesberg’s team are more alien, bearing little chemical resemblance to the four natural ones. In a 2008 paper, and in follow-up experiments, the group reported efforts to pair chemicals together from a list of 60 candidates and screen the 3,600 resulting combinations. They identified a pair of bases, known as d5SICS and dNaM, that looked promising. In particular, the molecules had to be compatible with the enzymatic machinery that copies and translates DNA.

(read more)

 


Via Marko Dolinar
more...
No comment yet.
Rescooped by Kartik Soni from Synthetic, Systems Biology and DNA Computing
Scoop.it!

Synthetic circuits integrating logic and memory in living cells

Synthetic circuits integrating logic and memory in living cells | Synbio | Scoop.it

Via Gerd Moe-Behrens, Alfonso Rodriguez-Paton
more...
Gerd Moe-Behrens's curator insight, February 10, 2013 5:40 PM

by
Piro Siuti,John Yazbek& Timothy K Lu

"Logic and memory are essential functions of circuits that generate complex, state-dependent responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits that use recombinases to implement Boolean logic functions with stable DNA-encoded memory of events. Application of this strategy allowed us to create all 16 two-input Boolean logic functions in living Escherichia coli cells without requiring cascades comprising multiple logic gates. We demonstrate long-term maintenance of memory for at least 90 cell generations and the ability to interrogate the states of these synthetic devices with fluorescent reporters and PCR. Using this approach we created two-bit digital-to-analog converters, which should be useful in biotechnology applications for encoding multiple stable gene expression outputs using transient inputs of inducers. We envision that this integrated logic and memory system will enable the implementation of complex cellular state machines, behaviors and pathways for therapeutic, diagnostic and basic science applications."

http://bit.ly/123ToU3

Rescooped by Kartik Soni from Synthetic, Systems Biology and DNA Computing
Scoop.it!

Advancing bacteriophage-based microbial diagnostics with synthetic biology

Advancing bacteriophage-based microbial diagnostics with synthetic biology | Synbio | Scoop.it

Via Gerd Moe-Behrens, Alfonso Rodriguez-Paton
more...
Gerd Moe-Behrens's curator insight, April 24, 2013 5:25 PM

by
Lu TK, Bowers J, Koeris MS.

"Synthetic biology is an emerging engineering field focused on designing artificial biological systems with novel func- tionalities for use in therapeutics, basic science, biotech- nology, and diagnostics [1,2]. Continuous advancements in DNA synthesis and sequencing technologies coupled with new techniques for genomic modification and assem- bly have opened the door for harnessing the power and diversity of biology for applications. For example, natural bacteriophage products, such as ListShield (Intralytix) and Agriphage (Omnilytics), are commercially available for reducing unwanted bacterial contamination. Natural bacteriophages can be genetically modified to deliver engineered payloads into bacteria, thus selectively func- tionalizing target bacterial populations to produce active biomolecules. This strategy can endow bacteriophages with the ability to efficiently destroy bacterial biofilms or increase the bactericidal efficacy of antibiotics used in combination with phages by many orders of magnitude [3]. In addition, bacteriophages can be engineered as near- real-time microbial diagnostics by using them to trans- form target bacteria into factories for detectable molecules...."
 http://bit.ly/XXyJ15

Rescooped by Kartik Soni from synthetic RNA biology
Scoop.it!

Synthetic Biology Delivers Cool Tools but New Therapeutics Are a Ways Off - Genetic Engineering News

Synthetic Biology Delivers Cool Tools but New Therapeutics Are a Ways Off - Genetic Engineering News | Synbio | Scoop.it
Genetic Engineering NewsSynthetic Biology Delivers Cool Tools but New Therapeutics Are a Ways OffGenetic Engineering NewsFor cancer research in particular, the field of syn bio could both reveal new insights into the disease and potentially lead to...

Via Puriney
more...
No comment yet.