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SCNT Picks Up Steam | The Scientist Magazine®

SCNT Picks Up Steam | The Scientist Magazine® | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Somatic cell nuclear transfer is now used in a therapeutic cloning procedure to generate embryonic stem cells from  a 32-year-old type 1 diabetic woman. The researchers developed an improved oocyte activation protocol, and used both kinase and translation inhibitors  and cell culture in the presence of histone deacetylase inhibitors. The diploid pluripotent stem-cell lines generated by this technique hold great promise for the future of diabetic regenerative medicine approaches. Read the paper: http://tinyurl.com/kabfk4z


Find more information on stem cell research in LifeMap Discovery:

http://discovery.lifemapsc.com/stem-cell-differentiation


 


 

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Scientists Are 3D Printing Whole Cancer Tumors From Scratch

Scientists Are 3D Printing Whole Cancer Tumors From Scratch | Stem Cells & Tissue Engineering | Scoop.it
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Scientists from Philadelphia have bioprinted a mixture of cancerous cell line called HeLa cells, and hydrogel to form 3D tumors. The HeLa cells line was generated from tumor cells taken from  Henrietta Lacks, in the 1950s and are considered as the first "immortal" line of research cells. 

Read the paper: http://iopscience.iop.org/1758-5090/6/3/035001/article

 

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Obokata's mentor cites 3 pieces of evidence supporting STAP cell existence

Obokata's mentor cites 3 pieces of evidence supporting STAP cell existence | Stem Cells & Tissue Engineering | Scoop.it
A co-author of what were once considered groundbreaking reports for regenerative medicine dismissed widening skepticism of the existence of the “stimulus-triggered acquisition of pluripotency” (STAP) phenomenon.
Jacob Blumenthal's insight:

Yoshiki Sasai, deputy director of the Riken Center for Developmental Biology (CDB) in Kobe, is one of the co-authors on the exciting papers on STAP cells. In a press conference he held, he said that he strongly believes that although some mistakes were done by the lead researcher Haruku Obokata, the STAP phenomenae is real. He mentioned  three reasons for that:

1. A live-cell imaging microscope automatically recorded the process of STAP cells generation, and therefore fabricating the images is very difficult.

2.  STAP cells are not embryonic stem (ES) cells contamination, since they are much smaller than ES cells. 

3. STAP cells were shown to generate not only a whole embryo but also the placenta, which ES cells can not form.

 

Sasai concluded that Obokata should reveal the full detailed procedure for STAP cells generation, including procedures that she may have used unconsciously but were significant in the procedure.

This is necessary in order for allow stem cell researchers to reproduce STAP cells in their own labs.

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Goodbye iPSCs Hello Cloning?

In what looks like a major scientific breakthrough, researchers reported in Cell Stem Cell a new technique for producing patient-specific embryonic stem cells via somatic-cell nuclear transfer (SCNT)
Jacob Blumenthal's insight:

In what looks like a major scientific breakthrough, researchers reported in Cell Stem Cell a new technique for producing patient-specific embryonic stem cells via somatic-cell nuclear transfer (SCNT). The researchers used a nuclear transfer method, previously developed by reproductive biologist Shoukhrat Mitalipov of Oregon Health and Science University, to fuse between fibroblast cells of 35- and 75-year-old males and enucleated oocytes. The fused egg, now containing the DNA of the mature fibroblasts, started to divide in response to a stimulation and within five or six days it develops into an embryo shaped like a hollow sphere, which contained embryonic stem cells in its interior. Researchers believe that the crucial step in the process is the waiting time before triggering the egg to start dividing. In this study the researchers waited two hours. This time window is probably crucial to initiate reprogramming events of the mature DNA in the egg, prior to its divisions.

This amazing achievement will allow to generate patient specific embryonic stem cells from the patient own mature cells and to use them in various cell therapy approaches. Unlike induced pluripotent stem cell (iPS) techniques, this procedure does not require any plasmids or viral reprogramming systems, and therefore its much safer. However, we shall wait until more labs and researchers will confirm that SCNT in humans can be reproduced, before throwing away any "old" reprogramming methods.

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Scientist Quits Effort to Live-Blog STAP Cell Replication

Scientist Quits Effort to Live-Blog STAP Cell Replication | Stem Cells & Tissue Engineering | Scoop.it
The Hong Kong-based scientist doesn't think STAP cells exist and wants to get back to his own research projects
Jacob Blumenthal's insight:

Perhaps Haruko Obokata should contact this scientist and offer him guidance in generating her STAP stem cells. She offered this kind of help in the press conference she held last week.

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Scientific Breakthrough as Artificial Blood is Created from Stem Cells

Scientific Breakthrough as Artificial Blood is Created from Stem Cells | Stem Cells & Tissue Engineering | Scoop.it
Mass production of human blood could become reality as artificial blood is tested on patients for the first time.
Jacob Blumenthal's insight:

British researchers have announced that after 4 years of research their Blood Pharma programme has made enough progress turning stem cells into red blood cells to move to clinical trials by late 2016. The researchers use both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) to generate blood cells in-vitro. They differentiate them into red blood cells, and expand their number in culture. The last differentiation stage is when the  developing blood cells eject their DNA to adopt the enucleated state characteristic of red blood cells.

Learn more about blood development and related stem cell differentiation protocols:

http://discovery.lifemapsc.com/in-vivo-development/blood

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Obokata Doesn’t Give an Inch in Stem-Cell Dispute - Japan Real Time - WSJ

Obokata Doesn’t Give an Inch in Stem-Cell Dispute - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Japanese researcher Haruko Obokata is trying to clarify statements from her news conference last week that had led to widespread criticism.
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GEN | News Highlights:Lab-Grown Vaginas a Success, Years after Patient Implantation

GEN | News Highlights:Lab-Grown Vaginas a Success, Years after Patient Implantation | Stem Cells & Tissue Engineering | Scoop.it
The first human recipients of lab-grown vaginas, after a number of annual followup evaluations, report normal sexual function, including desire and pain-free intercourse.
Jacob Blumenthal's insight:

Researchers led by Anthony Atala, M.D., director of Wake Forest Baptist Medical Center’s Institute for Regenerative Medicine, have developed a novel tissue engineering approach of engineering vaginal organs in the lab. The engineered vaginas were implanted in 4 women that were born with a rare form of vaginal aplasia, Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome.  In order to engineer the vaginas, the researchers grew cells on 3D biodegradable scaffolds, that upon transplantation allowed the entrance of blood vessels and nerve cells from the recipient's body into the scaffold. Now, after several years of follow up visits, the researchers conclude that these implants can be used successfully in human beings.

To the paper:  http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(14)60542-0/abstract

 

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Joshua Hunsberger's curator insight, April 17, 2014 2:02 PM

Lab-grown sex organs provide treatments.

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Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury

Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper in PNAS describes a novel tissue engineering method to repair soft tissue defects. Researchers from the lab of Prof. Shulamit Levenberg in the Technion, lead by Dr. Yulia Shandalov have fabricated an engineered muscle flap bearing its own functional vascular pedicle, and used it to repair a large soft tissue defect in mice. First, a 3D scaffold seeded with endothelial cells, fibroblasts, and myoblasts was prepared in-vitro. Then, it was implanted in-vivo around the femoral artery and veins before it was transferred to reconstruct an abdominal wall defect in the same mouse.

Read the full paper: 

http://www.pnas.org/content/early/2014/04/02/1402679111.long


* I was part of the Levenberg's lab between the years 2010-2012.



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Live: Obokata Speaks About Stem Cell Research Probe - Japan Real Time - WSJ

Live: Obokata Speaks About Stem Cell Research Probe - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Hundreds of journalists have descended on an Osaka hotel for a news conference by Haruko Obokata, the 30-year-old scientist accused of fabricating images in what initially appeared to be a breakthrough study on stem cells.
Jacob Blumenthal's insight:

Haruko Obokata defends her STAP cells research in a press conference today (Wednesday). 

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Japan Stem-Cell Researcher Obokata Is Hospitalized - Japan Real Time - WSJ

Japan Stem-Cell Researcher Obokata Is Hospitalized - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Japanese stem-cell researcher Haruko Obokata has been hospitalized because her "mental and physical condition is unstable," her lawyers said.
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Erasing a genetic mutation

Erasing a genetic mutation | Stem Cells & Tissue Engineering | Scoop.it
MIT team reverses a liver disorder in mice by correcting a mutated gene. (RT @drgautammehta: #Liver directed #gene therapy comes of age with CRISPR technology..
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Alzheimer’s research team employs stem cells to understand disease processes and study new treatment

Alzheimer’s research team employs stem cells to understand disease processes and study new treatment | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Researchers from the Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School have generated  induced pluripotent stem cell (iPSC) lines from patients harboring the London familial AD (fAD) amyloid precursor protein (APP) mutation (V717I). They found that during differentiation of the iPSC towards neuronal fate, APP expression and levels of Aβ increase dramatically. In addition, an increase in levels of total and phosphorylated Tau was also observed in neurons with the APPV717I mutation. Treating the neurons early in culture with Aβ-specific antibodies, reversed the phenotype of increased total Tau levels.

http://www.ncbi.nlm.nih.gov/pubmed/24524897



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Reprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors: Cell

Reprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors: Cell | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper, published in Cell, describes a novel method to reprogram mature committed blood cells from mice into blood-forming hematopoietic stem cells (HSCs), using a cocktail of six reprogramming factors:Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 . The reprogrammed cells, also termed iHSCs "possess clonal multilineage differentiation potential, reconstitute stem/progenitor compartments, and are serially transplantable. "

Learn about blood cells development in LifeMap Discovery:

http://discovery.lifemapsc.com/in-vivo-development/blood


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Adult Stem Cell Research Shows Promise - FDA

Adult Stem Cell Research Shows Promise - FDA | Stem Cells & Tissue Engineering | Scoop.it
FDA scientists are working on an unprecedented project. They are studying adult mesenchymal stem cells (MSCs), which are cells that can turn into a variety of cell types and tissues including those that form fat, cartilage and bone. MSC-based products have the potential to be used for cellular repair, restoration, replacement and regeneration if approved by FDA.
Jacob Blumenthal's insight:

The Food and Drug Administration (FDA) supports and investigates  adult stem cell research. The organization has established a consortium for the study of adult mesenchymal stem cells (MSCs)—cells that could eventually be used to repair, replace, restore or regenerate cells in the body, including those needed for heart and bone repair. These are very good news for patients in the US and in the entire world, waiting for the FDA approval on such adult stem cells therapies.

Stem cell researchers, interested to learn more about mesenchymal stem cells?  

Click : http://discovery.lifemapsc.com/in-vivo-development/mesenchymal-stem-cells

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3-D Printing Blood Vessels into Artificial Tissues Could Eliminate Need for Donor Organs | MIT Technology Review

3-D Printing Blood Vessels into Artificial Tissues Could Eliminate Need for Donor Organs | MIT Technology Review | Stem Cells & Tissue Engineering | Scoop.it
Artificial tissue has always lacked a key ingredient: blood vessels. A new 3-D printing technique seems poised to change that. (RT @MosesFeaster: What's artificial blood w/o artificial blood vessels?
Jacob Blumenthal's insight:

A team of Harvard researchers have created a tissue interlaced with blood vessels. "The tissue is the first made through 3-D printing to include potentially functional blood vessels embedded among multiple, patterned cell types".

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Joshua Hunsberger's curator insight, April 21, 2014 5:06 PM

3-D printing combining blood vessels & cell! A powerful combination.

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The Promise of Stem Cell Research - Dr. Paul Knoepfler

Dr. Paul Knoepfler from UC Davis School of Medicine and author of "Stem Cells - An Insider's Guide" discusses the research and future of stem cells. http://w...
Jacob Blumenthal's insight:

In this video interview Dr. Paul Knoepfler talks about stem cell therapy, induced pluripotent stem cells (iPSCs) and STAP stem cells.

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Why choosing the proper substrate is important for stem cell differentiation

Until now most of stem cell researchers overlooked the substrate they use for culture and differentiation of their stem cells. However, in the recent years it became clear that the physical and
Jacob Blumenthal's insight:

Until now most of stem cell researchers overlooked the substrate they use for culture and differentiation of their stem cells. However, in the recent years it became clear that the physical and chemical properties of the substrate affects stem cell differentiation efficiency and even the differentiation path itself. A new paper in Nature Materials reveals the effect of physical microenvironment on neural induction of human pluripotent stem cells (PSCs). The researchers differentiated human pluripotent stem cells into motor neurons on a synthetic microengineered substrate system consisting of poly(dimethylsiloxane) micropost arrays (PMAs). They found that the purity and yield of functional motor neurons derived from PSCs using soft PMAs were improved more than fourfold and tenfold, respectively, compared with coverslips or rigid PMAs. They found that the neuronal differentiation process is regulated by amulti-targeted mechanotransductive process involving Smad phosphorylation and nucleocytoplasmic shuttling.

This culture system will enable to produce more functional motor neurons than "classical" culturing methods, and by that will bring us a step closer to use these cells in clinical applications to treat amyotrophic lateral sclerosis (ALS) and other motor neuron-related diseases.

Link to the paper: http://tinyurl.com/nbffqu4

Learn how to differentiate stem cells into motor neurons: 

http://tinyurl.com/nkns7g7

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Where Will a Biology PhD Take You?

Where Will a Biology PhD Take You? | Stem Cells & Tissue Engineering | Scoop.it
Based primarily on the 2012 NIH Workforce report this infographic represents current workforce sizes and annual fluxes before and after a PhD in the b...
Jacob Blumenthal's insight:

This infographic represents the workforce sizes and annual fluxes before and after a PhD in the biomedical sciences in the US and is based on the 2012 NIH Workforce report. It shows that after post-doc studies (37-68,000 postdocs), 29,000 of researchers will be on a tenured track faculty position, or already tenured academy sciences. 17,000 will be PhD. scientists doing non-science jobs, and 22,555 will work as industry researchers. To which group you belong?

Learn more here: http://tinyurl.com/nrm973y

 

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Nose Rebuilt With Cartilage Grown From Patient's Own Body

Nose Rebuilt With Cartilage Grown From Patient's Own Body | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Researchers from Swiss have successfully performed nose reconstruction surgery with cartilage grown from the patient’s own tissue. First, the researchers isolated small biopsies from the nose of patients who suffer from severe nose defects from skin cancer surgery. Then, cartilage cells were isolated from this biopsies and cultured in-vitro. At the end of the expansion period, the samples had grown to 40 times their original size. The cultured tissues were then shaped according to the patient’s defect and implanted back to the patients.

The results were published in the Lancet journal: 

http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(14)60544-4/abstract


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Glioblastoma’s “stem-like” cells laid bare | Broad Institute of MIT and Harvard

Glioblastoma’s “stem-like” cells laid bare | Broad Institute of MIT and Harvard | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A multidisciplinary team of researchers and physicians from Massachusetts General Hospital, the Broad Institute, and the Klarman Cell Observatory have discovered using epigenomics that changes in four transcription factors  switch  non-aggressive brain tumor cells to a very aggressive brain tumor cells.

The paper was published in Cell: 

http://www.cell.com/cell/abstract/S0092-8674(14)00229-3


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Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells: Cell Stem Cell

Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells: Cell Stem Cell | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper in Cell Stem Cell describes a novel protocol for generation of stable immortalized megakaryocyte progenitor cell lines (imMKCLs) from human pluripotent stem cells (hPSC). The researchers derived imMKCLs from PSC-derived hematopoietic progenitors by overexpression of BMI1, BCL-XL , and c-MYC.

The derived  imMKCLs  have robust expansion capacity and can differentiate into mature megakaryocytes that release functional platelets. These characteristics makes them an ideal inexhaustible source of hPSC-derived platelets for clinical applications. 


Learn more about stem cells and differentiation protocols:

http://discovery.lifemapsc.com/stem-cell-differentiation


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NIH stem-cell programme closes

NIH stem-cell programme closes | Stem Cells & Tissue Engineering | Scoop.it
Director resigns as just one clinical-trial award is made.
Jacob Blumenthal's insight:

A news article published today in Nature, reveals that stem-cell biologist Mahendra Rao who was the director of CRM, left the NIH on 28 March. Established in 2010, CRM's goal was to promote and develop induced pluripotent stem (iPS) cells (adult cells that have been converted into embryonic-like stem cells) —based therapies. 

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Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming

Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

In this excellent review, the researchers describe the different reprogramming strategies that can give rise to cardiomyocytes for regenerative medicine purposes. They discuss induced pluripotent stem cell technology and direct reprogramming techniques.

Review: http://stemcellstm.alphamedpress.org/content/3/4/448.short?rss=1


Learn more about heart development and stem cell protocols:

http://discovery.lifemapsc.com/in-vivo-development/heart


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Stem cells and light restore paralysed muscles - YouTube

Full story: http://www.newscientist.com/article/dn25358 Stimulating neurons with light can restore movement to paralysed mouse muscles – a step towards...
Jacob Blumenthal's insight:

A new paper in Science describes a novel method to control muscle function, by transplantation of light-sensitive stem cell-derived motor neurons in mice.

First, the researchers generated mouse embryonic stem cell–derived motor neurons that express the light-sensitive ion channel channelrhodopsin-2. Then, these light-sensitive motor neurons were transplanted  into partially denervated branches of the sciatic nerve of adult mice. The transplanted cells were shown to re-innervate lower hind-limb muscles, and restored muscle functionality, when optogenic stimulation was applied.

http://www.sciencemag.org/content/344/6179/94

 

Join my Facebook group for more news and updates:

https://www.facebook.com/groups/STEMCELLSNET/

 

Learn more about motor neurons development, gene expression, and related stem cells differentiation protocols:

http://discovery.lifemapsc.com/in-vivo-development/motor-neurons

 

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