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Researchers regenerate retina in mice | Stem Cells Freak

Researchers regenerate retina in mice | Stem Cells Freak | Stem Cells & Tissue Engineering | Scoop.it

Researchers from the Centre for Genomic Regulation (CRG) in Barcelona have managed to regenerate the retina in mice using neuronal reprogramming. There are currently several lines of research that explore the possibility of tissue regeneration through cell reprogramming. One of the mechanisms being studied is reprogramming through cell fusion.


Jacob Blumenthal's insight:

In this paper, published in "Cell Reports", researchers demonstrate how

upon activation of Wnt/beta-catenin signaling, mouse retinal neurons can be
transiently reprogrammed in vivo back to a precursor stage. This occurs after their spontaneous fusion with transplanted hematopoietic stem and progenitor cells (HSPCs). Newly formed hybrids can proliferate, commit to differentiation toward a neuroectodermal lineage, and

finally develop into terminally differentiated neurons.

This is an open-access article:

http://www.cell.com/cell-reports/fulltext/S2211-1247(13)00293-3

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A Good Hair Day - an Exciting Breakthrough in Hair Formation

A Good Hair Day - an Exciting Breakthrough in Hair Formation | Stem Cells & Tissue Engineering | Scoop.it
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According to the American Hair Loss Association, two thirds of men by the age of 35, and 40% of women will experience hair loss. A new study performed by researchers from University of Pennsylvania and published in Nature Communications, may offer them some hope.

In this study, the researchers...(Click on the image for the full story).

 

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Where Will a Biology PhD Take You?

Where Will a Biology PhD Take You? | Stem Cells & Tissue Engineering | Scoop.it
Based primarily on the 2012 NIH Workforce report this infographic represents current workforce sizes and annual fluxes before and after a PhD in the b...
Jacob Blumenthal's insight:

This infographic represents the workforce sizes and annual fluxes before and after a PhD in the biomedical sciences in the US and is based on the 2012 NIH Workforce report. It shows that after post-doc studies (37-68,000 postdocs), 29,000 of researchers will be on a tenured track faculty position, or already tenured academy sciences. 17,000 will be PhD. scientists doing non-science jobs, and 22,555 will work as industry researchers. To which group you belong?

Learn more here: http://tinyurl.com/nrm973y

 

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Nose Rebuilt With Cartilage Grown From Patient's Own Body

Nose Rebuilt With Cartilage Grown From Patient's Own Body | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Researchers from Swiss have successfully performed nose reconstruction surgery with cartilage grown from the patient’s own tissue. First, the researchers isolated small biopsies from the nose of patients who suffer from severe nose defects from skin cancer surgery. Then, cartilage cells were isolated from this biopsies and cultured in-vitro. At the end of the expansion period, the samples had grown to 40 times their original size. The cultured tissues were then shaped according to the patient’s defect and implanted back to the patients.

The results were published in the Lancet journal: 

http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(14)60544-4/abstract


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Glioblastoma’s “stem-like” cells laid bare | Broad Institute of MIT and Harvard

Glioblastoma’s “stem-like” cells laid bare | Broad Institute of MIT and Harvard | Stem Cells & Tissue Engineering | Scoop.it
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A multidisciplinary team of researchers and physicians from Massachusetts General Hospital, the Broad Institute, and the Klarman Cell Observatory have discovered using epigenomics that changes in four transcription factors  switch  non-aggressive brain tumor cells to a very aggressive brain tumor cells.

The paper was published in Cell: 

http://www.cell.com/cell/abstract/S0092-8674(14)00229-3


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Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells: Cell Stem Cell

Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells: Cell Stem Cell | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper in Cell Stem Cell describes a novel protocol for generation of stable immortalized megakaryocyte progenitor cell lines (imMKCLs) from human pluripotent stem cells (hPSC). The researchers derived imMKCLs from PSC-derived hematopoietic progenitors by overexpression of BMI1, BCL-XL , and c-MYC.

The derived  imMKCLs  have robust expansion capacity and can differentiate into mature megakaryocytes that release functional platelets. These characteristics makes them an ideal inexhaustible source of hPSC-derived platelets for clinical applications. 


Learn more about stem cells and differentiation protocols:

http://discovery.lifemapsc.com/stem-cell-differentiation


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NIH stem-cell programme closes

NIH stem-cell programme closes | Stem Cells & Tissue Engineering | Scoop.it
Director resigns as just one clinical-trial award is made.
Jacob Blumenthal's insight:

A news article published today in Nature, reveals that stem-cell biologist Mahendra Rao who was the director of CRM, left the NIH on 28 March. Established in 2010, CRM's goal was to promote and develop induced pluripotent stem (iPS) cells (adult cells that have been converted into embryonic-like stem cells) —based therapies. 

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Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming

Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

In this excellent review, the researchers describe the different reprogramming strategies that can give rise to cardiomyocytes for regenerative medicine purposes. They discuss induced pluripotent stem cell technology and direct reprogramming techniques.

Review: http://stemcellstm.alphamedpress.org/content/3/4/448.short?rss=1


Learn more about heart development and stem cell protocols:

http://discovery.lifemapsc.com/in-vivo-development/heart


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Stem cells and light restore paralysed muscles - YouTube

Full story: http://www.newscientist.com/article/dn25358 Stimulating neurons with light can restore movement to paralysed mouse muscles – a step towards...
Jacob Blumenthal's insight:

A new paper in Science describes a novel method to control muscle function, by transplantation of light-sensitive stem cell-derived motor neurons in mice.

First, the researchers generated mouse embryonic stem cell–derived motor neurons that express the light-sensitive ion channel channelrhodopsin-2. Then, these light-sensitive motor neurons were transplanted  into partially denervated branches of the sciatic nerve of adult mice. The transplanted cells were shown to re-innervate lower hind-limb muscles, and restored muscle functionality, when optogenic stimulation was applied.

http://www.sciencemag.org/content/344/6179/94

 

Join my Facebook group for more news and updates:

https://www.facebook.com/groups/STEMCELLSNET/

 

Learn more about motor neurons development, gene expression, and related stem cells differentiation protocols:

http://discovery.lifemapsc.com/in-vivo-development/motor-neurons

 

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Patient stem cells help identify common problem in ALS

Patient stem cells help identify common problem in ALS | Stem Cells & Tissue Engineering | Scoop.it
Harvard stem cell scientists have discovered that a recently approved medication for epilepsy may possibly be a meaningful treatment for amyotrophic lateral sclerosis (ALS)—Lou Gehrig's disease, a uniformly fatal neurodegenerative disorder. The researchers are now collaborating with Massachusetts ...
Jacob Blumenthal's insight:

In two papers, published in Cell Reports, and in Cell Stem Cell, researchers used induced pluripotent stem cells (iPSC) derived from Amyotrophic Lateral Sclerosis (ALS) patients cells to study  aberrant molecular mechanisms related to ALS  in-vitro. In the first paper, researchers from the labs of Kevin Eggan and Clifford Woolf found that iPSC-derived motor neurons from ALS patients are hyperexcitable compared to controls, and that the drug Retigabine,  which has recently been approved as an anticonvulsive, could rescues the hyperexcitability phenotype in multiple ALS variants.

In the second paper, researchers from the lab of Su-Chun Zhang found that neurofilament aggregation is an early phenotype of ALS, and that correction of  neurofilament expression, mitigates axonal degeneration in ALS motor neurons.

http://www.cell.com/cell-reports/abstract/S2211-1247(14)00197-1

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(14)00055-1

 

Learn about motor neurons development, pathologies, gene expression and related motor neurons derivation protocols:

http://discovery.lifemapsc.com/in-vivo-development/motor-neurons

 

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Family Guy -- Why Are We Not Funding This (Stem Cell Research) - YouTube

Jacob Blumenthal's insight:

A nice animation on YouTube :)

 

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Joshua Hunsberger's curator insight, April 3, 12:43 PM

I ask myself the same question. Why are we not providing more funding for Stem Cell Research. The potential is there to deliver cures. 

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'Phony' Stem Cell Researcher To Be Punished : DNews

'Phony' Stem Cell Researcher To Be Punished : DNews | Stem Cells & Tissue Engineering | Scoop.it
The move is a huge blow to what was touted as a game-changing discovery.
Jacob Blumenthal's insight:

"Riken Institute head, Ryoji Noyori, who jointly won the Nobel Prize for chemistry in 2001, said in a statement he will "rigorously punish relevant people after procedures in a disciplinary committee."

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Mismatch alleged in acid-bath stem-cell experiment

Mismatch alleged in acid-bath stem-cell experiment | Stem Cells & Tissue Engineering | Scoop.it
Cell lines linked to blockbuster papers came from different mouse strain to that claimed, study suggests.
Jacob Blumenthal's insight:

Concerns were raised about the origin of two stem cell lines, that were produced by Haruko Obokata in the STAP stem cells paper.

Researchers suspect that the two stem cell lines were not produced from a single mouse strain, as Obokata wrote, but from several lines. 

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New stem cell technique bypasses ethical concerns

New stem cell technique bypasses ethical concerns | Stem Cells & Tissue Engineering | Scoop.it
Stem cell and cloning research using human cells remain controversial, even though it is nearly 20 years since Dolly the sheep became the first mammal to be cloned. One of the main reasons for this controversy…
Jacob Blumenthal's insight:

In a new paper, published in Nature, researchers led by Shoukhrat Mitalipov, have demonstrated a novel nuclear reprogramming technique in mice. Until now, unfertilized eggs were needed for cell cloning by nuclear reprogramming. In the new technique, the researchers used fertilized eggs at the stage of interphase, just after the first cell division. They injected nuclei of two non-reproductive cells into both cells of the two-cell embryo that has just undergone its first cell division. During this stage, the conditions allowed formation of embryonic stem cells or even a clone. If these results could be reproduced in human, it will allow to generate stem cells from any person, without the need to destroy embryos, as in the case of human embryonic stem cells.

Paper: http://www.nature.com/nature/journal/vaop/ncurrent/full/nature13134.html

 

Learn more about stem cells and their differentiation protocols: 

http://discovery.lifemapsc.com/stem-cell-differentiation

 

 

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Scientific Breakthrough as Artificial Blood is Created from Stem Cells

Scientific Breakthrough as Artificial Blood is Created from Stem Cells | Stem Cells & Tissue Engineering | Scoop.it
Mass production of human blood could become reality as artificial blood is tested on patients for the first time.
Jacob Blumenthal's insight:

British researchers have announced that after 4 years of research their Blood Pharma programme has made enough progress turning stem cells into red blood cells to move to clinical trials by late 2016. The researchers use both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) to generate blood cells in-vitro. They differentiate them into red blood cells, and expand their number in culture. The last differentiation stage is when the  developing blood cells eject their DNA to adopt the enucleated state characteristic of red blood cells.

Learn more about blood development and related stem cell differentiation protocols:

http://discovery.lifemapsc.com/in-vivo-development/blood

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Obokata Doesn’t Give an Inch in Stem-Cell Dispute - Japan Real Time - WSJ

Obokata Doesn’t Give an Inch in Stem-Cell Dispute - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Japanese researcher Haruko Obokata is trying to clarify statements from her news conference last week that had led to widespread criticism.
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GEN | News Highlights:Lab-Grown Vaginas a Success, Years after Patient Implantation

GEN | News Highlights:Lab-Grown Vaginas a Success, Years after Patient Implantation | Stem Cells & Tissue Engineering | Scoop.it
The first human recipients of lab-grown vaginas, after a number of annual followup evaluations, report normal sexual function, including desire and pain-free intercourse.
Jacob Blumenthal's insight:

Researchers led by Anthony Atala, M.D., director of Wake Forest Baptist Medical Center’s Institute for Regenerative Medicine, have developed a novel tissue engineering approach of engineering vaginal organs in the lab. The engineered vaginas were implanted in 4 women that were born with a rare form of vaginal aplasia, Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome.  In order to engineer the vaginas, the researchers grew cells on 3D biodegradable scaffolds, that upon transplantation allowed the entrance of blood vessels and nerve cells from the recipient's body into the scaffold. Now, after several years of follow up visits, the researchers conclude that these implants can be used successfully in human beings.

To the paper:  http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(14)60542-0/abstract

 

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Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury

Technion 'breakthrough': Transplanting of engineered tissue to repair muscle injury | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A new paper in PNAS describes a novel tissue engineering method to repair soft tissue defects. Researchers from the lab of Prof. Shulamit Levenberg in the Technion, lead by Dr. Yulia Shandalov have fabricated an engineered muscle flap bearing its own functional vascular pedicle, and used it to repair a large soft tissue defect in mice. First, a 3D scaffold seeded with endothelial cells, fibroblasts, and myoblasts was prepared in-vitro. Then, it was implanted in-vivo around the femoral artery and veins before it was transferred to reconstruct an abdominal wall defect in the same mouse.

Read the full paper: 

http://www.pnas.org/content/early/2014/04/02/1402679111.long


* I was part of the Levenberg's lab between the years 2010-2012.



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Live: Obokata Speaks About Stem Cell Research Probe - Japan Real Time - WSJ

Live: Obokata Speaks About Stem Cell Research Probe - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Hundreds of journalists have descended on an Osaka hotel for a news conference by Haruko Obokata, the 30-year-old scientist accused of fabricating images in what initially appeared to be a breakthrough study on stem cells.
Jacob Blumenthal's insight:

Haruko Obokata defends her STAP cells research in a press conference today (Wednesday). 

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Japan Stem-Cell Researcher Obokata Is Hospitalized - Japan Real Time - WSJ

Japan Stem-Cell Researcher Obokata Is Hospitalized - Japan Real Time - WSJ | Stem Cells & Tissue Engineering | Scoop.it
Japanese stem-cell researcher Haruko Obokata has been hospitalized because her "mental and physical condition is unstable," her lawyers said.
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Erasing a genetic mutation

Erasing a genetic mutation | Stem Cells & Tissue Engineering | Scoop.it
MIT team reverses a liver disorder in mice by correcting a mutated gene. (RT @drgautammehta: #Liver directed #gene therapy comes of age with CRISPR technology..
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Jacob Blumenthal's curator insight, April 6, 2:58 AM

Using a new gene-editing system based on bacterial proteins, known as CRISPR,  MIT researchers have cured mice of a rare liver disorder caused by a single genetic mutation.

This technique offers an easy way to replace a mutated DNA sequence, with a correct sequence. 

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Alzheimer’s research team employs stem cells to understand disease processes and study new treatment

Alzheimer’s research team employs stem cells to understand disease processes and study new treatment | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Researchers from the Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School have generated  induced pluripotent stem cell (iPSC) lines from patients harboring the London familial AD (fAD) amyloid precursor protein (APP) mutation (V717I). They found that during differentiation of the iPSC towards neuronal fate, APP expression and levels of Aβ increase dramatically. In addition, an increase in levels of total and phosphorylated Tau was also observed in neurons with the APPV717I mutation. Treating the neurons early in culture with Aβ-specific antibodies, reversed the phenotype of increased total Tau levels.

http://www.ncbi.nlm.nih.gov/pubmed/24524897



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Molly Stevens: A new way to grow bone - YouTube

What does it take to regrow bone in mass quantities? Typical bone regeneration — wherein bone is taken from a patient's hip and grafted onto damaged bone els...
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Molly stevens talks about bone regeneration in TED.

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RIKEN Panel Finds Misconduct in Reprogrammed Stem Cell Papers

RIKEN Panel Finds Misconduct in Reprogrammed Stem Cell Papers | Stem Cells & Tissue Engineering | Scoop.it
Investigating committee identifies two instances of research misconduct, puts most of the blame on lead author
Jacob Blumenthal's insight:

"

From the news : "An investigating committee has concluded that falsification and fabrication mar two recent Nature papers reporting a new, simple way to reprogram mature cells into stem cells. The committee concluded that these acts constitute research misconduct, but it stopped short of calling for the papers to be retracted and will leave the question of disciplinary action to a separate committee. RIKEN President Ryoji Noyori today said he favors the papers’ retraction if the committee’s findings are upheld in an appeals process.

“I am filled with feelings of indignation and surprise,” said lead author Haruko Obokata, of the RIKEN Center for Developmental Biology (RIKEN CDB) in Kobe, in a statement. She wrote that she intends to appeal the judgment".

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Joshua Hunsberger's curator insight, April 3, 12:45 PM

I say what can we learn from this and let's move forward.

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Stap cells: research paper on stem cell breakthrough was partly falsified

Stap cells: research paper on stem cell breakthrough was partly falsified | Stem Cells & Tissue Engineering | Scoop.it
Investigators at Japanese laboratory say lead author manipulated presentation of results to improve their appearance
Jacob Blumenthal's insight:

The STAP cells saga never ends, now researchers from Japan say that "researcher Haruko Obokata, the lead author of the paper in Nature, had manipulated or falsified images of DNA fragments used in the research".

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First comprehensive atlas of human gene activity released - A new road map for direct cell reprogramming?

First comprehensive atlas of human gene activity released - A new road map for direct cell reprogramming? | Stem Cells & Tissue Engineering | Scoop.it
A large international consortium of researchers has produced the first comprehensive, detailed map of the way genes work across the major cells and tissues of the human body. The findings describe the complex networks that govern gene activity, and the new information could play a crucial role in identifying the genes involved with disease.
Jacob Blumenthal's insight:

This new map of gene activity in cells and tissues, may serve as a road map for direct reprogramming of somatic cells. Knowing the master regulators, key genes for each lineage is crucial in order to perform efficient reprogramming.

Overall, it brings us closer to the times when researchers will be able to generate mature cells for regenerative medicine purposes from the patient's own cells, without the need to generate fully pluripotent stem cells.

Interested in tissue/organ specific gene expression? check the LifeMap Discovery database:  http://discovery.lifemapsc.com/in-vivo-development

 

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Joshua Hunsberger's curator insight, April 3, 12:06 PM

This is definitely worth investigating!