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Human embryonic stem cell derived islet progenitors mature inside an encapsulation device without evidence of increased biomass or cell escape

Human embryonic stem cell derived islet progenitors mature inside an encapsulation device without evidence of increased biomass or cell escape | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

This  new open-access paper, published in stem cell research journal, describes the encapsulation of human embryonic stem cells (hESC)-derived pancreatic islets, and their ability to produce insulin in response to glucose levels. Insulin production is achieved without increasing the cell mass, or the escape of the cells from the capsule.

This novel method will enable to minimize the risk of immune response against the  hESC-derived pancreatic cells.

 

To learn more about stem cell differentiation protocols, follow the link:

 http://discovery.lifemapsc.com/stem-cell-differentiation/protocols

 

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Researchers isolate and grow pancreatic stem cells | Stem Cells Freak

Researchers isolate and grow pancreatic stem cells | Stem Cells Freak | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

A research team from the Netherlands led by Dr. Hans Clevers, isolated and grew adult stem cells from the pancreases of mice using a 3-D culture system and studied their in-vitro differentiation potential. Lgr5, a receptor for the Wnt-agonistic R-spondins (RSPOs), serves as an adults stem cells marker in multiple adult organs. Under normal conditions the Wnt signalling pathway is inactive and Lgr5 is not expressed. However, under pathological conditions, such as injury, the Wnt signalling pathway is robustly activated, concomitant with Lgr5 expression in regenerating pancreatic ducts. When cultured in-vitro, mouse pancreatic duct fragmens form cyst-like structures, termed organoids. By modulating the in-vitro culturing conditions, the research showed that they can control the differentiation faith of these pancreatic organoids either towards the generation of pancreatic duct cells or towards the formation hormone-secreting endocrine-like cells.
This interesting paper demonstrates the bi-potent nature of pancreatic Lgr5+ cells, and the next step will be to show that human pancreatic stem cells behave the same way and have the same differentiation potential.


To the open-access paper:

http://www.nature.com/emboj/journal/vaop/ncurrent/full/emboj2013204a.html


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To study pancreas development: 

http://discovery.lifemapsc.com/in-vivo-development/pancreas


For stem cell differentiation protocols:

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols

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Carlos Garcia Pando's curator insight, September 18, 2013 5:48 AM

How long until we can manufacture functional pancreatic islands to implant? How will insulin manufacturers try to impede this happening?

Jacob Blumenthal's comment, September 18, 2013 6:31 AM
According to Doug Melton, in his 2013 ISSCR talk, there is no problem to generate millions of beta cells from embryonic stem cells. The main obstecle is how to encapsulate them upon administration to prevent the immune rejection response. I guess that the next step depends on bioengineering and biomaterials researchers.
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Mini-pancreas grown from stem cells has implications for future insulin delivery

Mini-pancreas grown from stem cells has implications for future insulin delivery | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

In a paper, published in Development journal, researchers from the  Danish Stem Cell Centre have shown that they can culture mouse pancreatic cells into expanded, branched structures in a 3D culture.

By manipulating the medium composition they  generated either hollow spheres, which are mainly composed of pancreatic progenitors, or complex organoids that spontaneously undergo pancreatic morphogenesis and differentiation.

Manipulation and improvement of  culture and expansion techniques 

 could lead to a more efficient and  developed method of creating insulin-releasing beta cells, which will eventually be used for cell therapy of diabetes.

http://dev.biologists.org/content/140/21/4452.full

 

To learn about pancreatic development:

http://discovery.lifemapsc.com/in-vivo-development/pancreas

 

To learn about stem cells differentiation protocols:

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols

 

 

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Carlos Garcia Pando's curator insight, October 16, 2013 2:04 PM

Another wonder result from tissue engineering!

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Pluripotent Stem Cells Made From Pancreatic Cancer Cells Are First Human ... - Newswise (press release)

Pluripotent Stem Cells Made From Pancreatic Cancer Cells Are First Human ... - Newswise (press release) | Stem Cells & Tissue Engineering | Scoop.it
Pluripotent Stem Cells Made From Pancreatic Cancer Cells Are First Human ...
Newswise (press release)
They have been searching for a human-cell model of early-disease progression.
Jacob Blumenthal's insight:

Scientist have created an iPS cell line from pancreatic cancer cells to model cancer progression.

http://www.cell.com/cell-reports/fulltext/S2211-1247(13)00269-6

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