Stem Cells & Tissue Engineering
50.6K views | +0 today
Stem Cells & Tissue Engineering
Stem Cells & Tissue Engineering  news and updates
Your new post is loading...
Your new post is loading...
Scooped by Jacob Blumenthal
Scoop.it!

Cardiac Fibroblasts - A Two-edged Sword?

Cardiac Fibroblasts - A Two-edged Sword? | Stem Cells & Tissue Engineering | Scoop.it
In many cardiac pathologies, cardiac muscle is severely impaired and is replaced by a scar tissue manifested by cardiac fibroblasts in a process called fibrosis. Cardiac muscle scarring leads to its
Jacob Blumenthal's insight:

Although it is not related to the stem cell field, thought it might interest this community. It deals about cardiac fibroblasts role in development and in pathology.

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Cell Stem Cell - Reprogramming toward Heart Regeneration: Stem Cells and Beyond

Jacob Blumenthal's insight:

This is a free review (for now...) from Cell Stem Cells, about the study of  regenerative mechanisms in the heart. It discusses the use of endogenous adult stem cell populations alongside the recent application of reprogramming technologies  for the development of therapies targeting heart disease.

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Regenerative Medicine Research | Current status of induced pluripotent stem cells in cardiac tissue regeneration and engineering

In this open-access short review, Liu. et al, discuss the achievements and current status of induced pluripotent stem cells (iPSC) for cardiac tissue regeneration, including development of iPSC derivation, in vitro strategies for cardiac generation from iPSCs, cardiac application of iPSCs, challenges confronted at present as well as perspective in the future. 

 

To learn  about heart development, stem cell differentiation protocols, and cell therapies:

http://discovery.lifemapsc.com/in-vivo-development/heart

 

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Stem Cell Reports - Direct Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State

Stem Cell Reports - Direct Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State | Stem Cells & Tissue Engineering | Scoop.it

Summary: Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficient in human fibroblasts, at least in vitro. Here, we show that GMT plus ESRRG and MESP1 induced global cardiac gene-expression and phenotypic shifts in human fibroblasts derived from embryonic stem cells, fetal heart, and neonatal skin. Adding Myocardin and ZFPM2 enhanced reprogramming, including sarcomere formation, calcium transients, and action potentials, although the efficiency remained low. Human iCM reprogramming was epigenetically stable. Furthermore, we found that transforming growth factor β signaling was important for, and improved the efficiency of, human iCM reprogramming. These findings demonstrate that human fibroblasts can be directly reprogrammed toward the cardiac lineage, and lay the foundation for future refinements in vitro and in vivo.

Jacob Blumenthal's insight:

In this paper, the researchers use direct differentiation methods to differentiate fibroblasts into cardiomyocyte-like cells. First, they do a screening analysis to find the most effective cocktail of transcription factor for the reprogramming. Then, they explore the gene expression of the induced cardiomtocytes, including their epigenetic state. Taken together, this paper adds important information regarding the transcription factors and signal pathways that are important to establish the identity of cardiomyocytes, which may lead in the future to the development of patient-specific therapies for heart diseases.

---------------------------------------------------------------------------------------------

To learn more about the development of the heart and cardiomyocytes:

http://discovery.lifemapsc.com/in-vivo-development/heart

 

To explore stem cells differentiation protocols towards cardiomyocytes :

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Common stem cell in heart and lung development explains adaption for life on land

Common stem cell in heart and lung development explains adaption for life on land | Stem Cells & Tissue Engineering | Scoop.it

In a new paper published this week online in Nature, a team from the Perelman School of Medicine, University of Pennsylvania, shows that the pulmonary vasculature, the blood vessels that connect the heart to the lung, develops even in the absence of the lung. Mice in which lung development is inhibited still have pulmonary blood vessels, which revealed to the researchers that cardiac progenitors, or stem cells, are essential for cardiopulmonary co-development.

Jacob Blumenthal's insight:

In a paper published online in Nature, the researchers show that the murine pulmonary vasculature develops even in the absence of lung development. They have identified a population of multipotent cardiopulmonary mesoderm progenitors (CPPs) within the posterior pole of the heart that are marked by the expression of Wnt2, Gli1 and Isl1. In addition, they demonstrated that CPPs arise from cardiac progenitors before lung development. They found that CPPs are regulated by hedgehog expression from the foregut endoderm, which is required for connection of the pulmonary vasculature to the heart. 

http://www.nature.com/nature/journal/vaop/ncurrent/full/nature12358.html

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Tissue engineering: How to build a heart

Tissue engineering: How to build a heart | Stem Cells & Tissue Engineering | Scoop.it
With thousands of people in need of heart transplants, researchers are trying to grow new organs.
more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Is heart regeneration on the right track?NATURE MEDICINE | BETWEEN BEDSIDE AND BENCH

Is heart regeneration on the right track?NATURE MEDICINE | BETWEEN BEDSIDE AND BENCH | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

From the abstract: "In 'Bedside to Bench', Christine L. Mummery and Richard T. Lee lay out a framework for re-evaluating cardiac cell therapies in the context of two recent clinical trials, in which autologous cardiac stem cells derived from heart biopsies were transferred into patients, with promising, albeit difficult to interpret, results.  In 'Bench to Bedside', Young-Jae Nam, Kunhua Song and Eric N. Olson discuss a number of recent studies in rodents showing that cardiac fibroblasts can be reprogrammed, via miRNAs and a transcription factor 'cocktail', to express cardiac genes, which resulted in improved cardiac function in the animals, suggesting a new way forward for fixing damaged heart tissue."

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming

Concise Review: Reprogramming Strategies for Cardiovascular Regenerative Medicine: From Induced Pluripotent Stem Cells to Direct Reprogramming | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

In this excellent review, the researchers describe the different reprogramming strategies that can give rise to cardiomyocytes for regenerative medicine purposes. They discuss induced pluripotent stem cell technology and direct reprogramming techniques.

Review: http://stemcellstm.alphamedpress.org/content/3/4/448.short?rss=1


Learn more about heart development and stem cell protocols:

http://discovery.lifemapsc.com/in-vivo-development/heart


more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Defining Key Design Criteria for Cardiac Tissue Engineering

Defining Key Design Criteria for Cardiac Tissue Engineering | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

Cardiomyocytes, differentiated from either human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs), are used in cardiovascular research throughout the last decade. In addition to their regenerative capacities and promise for clinical application, these cells provide an unlimited source of...

(click the link below for the full story)

http://us4.campaign-archive1.com/?u=985051700e9649000fa0c0d4a&id=ed2c6547b5&e=f1b990f7f8

more...
Carlos Garcia Pando's curator insight, December 5, 2013 3:18 AM

This is like the drawings and procedure. Sience is taking the engineering steps.

Scooped by Jacob Blumenthal
Scoop.it!

Packaging stem cells in capsules for heart therapy

Packaging stem cells in capsules for heart therapy | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

When stem cells are used for the treatment of heart diseases, they usually have a short-term effect, mostly because they don't stay at the injection site or simply because they die. In order to overcome this problem, researchers from Emory university encapsulated mesenchymal stem cells (MSCs) in alginate-based capsules and inserted them into heart-attack rat models. They found that the rats that were treated with the encapsulated cells displayed increased heart function, reduced scar size and more growth of new blood vessels , compared to control rats that were treated by standard cell injection. In addition. more encapsulated cells remained viable.

This strategy can be used for other cell therapies, like transplantation of pluripotent stem cells-derived beta islets for the treatment of diabetes. Of course, in that case the main challenge will be to prevent the rejection of these cells, post transplantation.

The paper was published in the Journal of American Heart Association: http://jaha.ahajournals.org/content/2/5/e000367.full

more...
No comment yet.
Scooped by Jacob Blumenthal
Scoop.it!

Is this the future of organ donation? Scientists grow beating mouse heart made from HUMAN stem cells

Is this the future of organ donation? Scientists grow beating mouse heart made from HUMAN stem cells | Stem Cells & Tissue Engineering | Scoop.it
Scientists from the University of Pittsburgh believe their breakthrough could lead to the development of transplant organs for patients thanks to stem cells produced from simple skin biopsies.
Jacob Blumenthal's insight:

Scientists generated heart constructs by repopulating decellularized mouse hearts with human induced pluripotent stem cell-derived multipotential cardiovascular progenitor cells. The cells migrate, proliferate and differentiate in situ into cardiomyocytes, smooth muscle cells and endothelial cells to reconstruct the decellularized hearts. After 20 days of perfusion, the engineered heart tissues exhibit spontaneous contractions, generate mechanical force and are responsive to drugs. This very interesting paper demonstrates the vast potential of the combination between tissue engineering 3D scaffolds, and stem cells differentiation. It was shown by others that decellularized tissues contain growth factor "pockets" or other imprinted csignals that allows recellularization of the tissue and differentiation of stem cells.

http://www.nature.com/ncomms/2013/130813/ncomms3307/full/ncomms3307.html


---------------------------------------------------------------------------------------------------

To learn about the embryonic development of the heart:

http://discovery.lifemapsc.com/in-vivo-development/heart


Stem cells differentiation protocols: 

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols

 


more...
Letícia Oliver Fernandes's curator insight, August 14, 2013 10:59 AM

Esta matéria, do jornal inglês Daily Mail, relata um experimento feito por cientistas ao trocar o coração de um rato por um coração feito a partir de células tronco, obtendo sucesso ao fazer com que voltasse a bater. Espera-se que, a partir desta experiência, seja possível desenvolver órgãos para transplante.

Scooped by Jacob Blumenthal
Scoop.it!

CHEN2820 / Myocardial tissue engineering

CHEN2820 / Myocardial tissue engineering | Stem Cells & Tissue Engineering | Scoop.it
Jacob Blumenthal's insight:

This is a very good summary of tissue engineering strategies to treat heart disease, in specific myocardial infarction.

 

more...
Sebastien Dazy's comment, July 12, 2013 8:30 AM
you should add hydrogel stent made of positive charged biopolymers derived from chitin as scaffold to next generation of myocardial tissue engineering.
Jacob Blumenthal's comment, July 14, 2013 1:56 AM
Thanks Sebastien
Scooped by Jacob Blumenthal
Scoop.it!

New Hope for Heart Repair: Direct reprogramming of cardiomyocytes

Scientists in San Francisco have coaxed mouse hearts to repair themselves from within. The breakthrough could lead to treatments for 5 million people in the ...
Jacob Blumenthal's insight:
The Method of the Year 2012 – direct reprogramming (transdifferentiation) in vivo

http://stemcellassays.com/2012/12/method-year-2012-direct-transdifferentiation-reprogramming-vivo/

more...
No comment yet.